Labshake search
Citations for New England Biolabs :
8301 - 8350 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 500 ng total RNA was used as template with the Luna Universal One-step RT-qPCR kit (New England Biolabs, E3005L). For each condition 3 biological replicates with two technical replicate RT-qPCR reactions were run on a CFX96 Connect Real-Time System (BioRad) ...
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bioRxiv - Developmental Biology 2024Quote: ... Ribosome-depleted RNA was used to make cDNA libraries using NEBNext Ultra II RNA Library preparation kit for Illumina and NEBNext Multiplex Oligos for Illumina as per manufacturer’s protocol (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA libraries were prepared by reverse transcribed total RNA using a ProtoScript® II First Strand cDNA Synthesis Kit (NEB, #E6560) with d(T)23 VN oligo and sent to Novogene for sequencing using Illumina Novaseq 6000 (PE 150bp with 6Gb read depth coverage).
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bioRxiv - Molecular Biology 2024Quote: ... Riboprobe for the full-length ACT1 gene was made using “HiScribe™ T7 Quick High Yield RNA Synthesis Kit” (NEB, USA) using Fluorescein labelling mix (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNA samples were converted to sequencing libraries using the NEBNext Small RNA Library Prep kit for Illumina (New England Biolabs, E7300) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was incubated for 3 days at 37C in the dark for conjugation and purified for 3 rounds using Monarch PCR & DNA Cleanup Kit (5 µg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Pathogenic ADA2 variants were created by site-directed mutagenesis using the Q5® Site-Directed Mutagenesis Kit (#E0554; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... Strand specific mRNA libraries were generated using the NEBNext Ultra II Directional RNA library prep Kit for Illumina (New England BioLabs #E7760), mRNA was isolated using Poly(A ...
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bioRxiv - Genomics 2024Quote: ... 1 µg of genomic DNA was pooled with 1:20 dilutions of unmethylated lambda (1 µl of 0.1 ng/µl) and methylated pUC19 control DNA (1 ul of 0.005 ng/µl) from the EM-seq kit (E7120L; NEB, Ipswich, MA). Volumes were made up to 50 ul with 0.1x TE buffer (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The RNA-Seq library-preparation protocol was based on the NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, #E7530L). Insert size was assessed using the Agilent Bioanalyzer 2100 system and qualified insert size was accurate quantification using StepOnePlus™ Real-Time PCR System (Library valid concentration>10nM ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR and digest products were purified using Monarch DNA Gel Extraction and PCR and DNA Clean Up Kits (NEB T1020S, T1030S).
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bioRxiv - Molecular Biology 2024Quote: Plasmids (pBluescript KS+, pBS hereon) for HiBit-tagged POI mRNA were generated by Gibson Assembly (Gibson Assembly cloning kit, NEB, E5510S) or restriction digest cloning ...
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bioRxiv - Molecular Biology 2024Quote: Nuclear RNA-seq libraries were prepared from DNA-free nuclear RNA using NEBNext® UltraTM II Directional RNA Library Prep Kit (NEB) after first depleting rRNA using the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) ...
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bioRxiv - Molecular Biology 2024Quote: ... Linearized DNAs were used as templates for in vitro transcription of capped RNAs with HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs) in the presence of CleanCap® Reagent AU (TriLink Biotechnologies) ...
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bioRxiv - Neuroscience 2024Quote: ... was used to construct sequencing libraries using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, NEB). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (dual index primers ...
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bioRxiv - Neuroscience 2024Quote: ... 5 ng of ChIP and Input DNA was used to generate ChIP-seq libraries using the NEBNext Ultra DNA library prep kit for Illumina (New England Biolabs, NEB).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Reverse transcription (1 µg total RNA or 500 ng at lower concentrations) was performed using the ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560L), resulting in cDNA concentrations of 50 and 25 ng/µL ...
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bioRxiv - Microbiology 2020Quote: ... bacilliformis total RNA (for each 25 µl reaction) collected from various conditions using the Luna Universal One-Step RT-qPCR kit (New England BioLabs; Ipswich, MA) according to the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids from non-motile isolates with straightened flagella were purified using the NEB monarch DNA mini prep kit (NEB, Ipswich, MA) and sequenced by Sanger sequencing using the primers (5-GGCACGAATTCCGAGCTGACGACCGTTCAG-3’ ...
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bioRxiv - Plant Biology 2020Quote: ... All mutated YFP-CESA6 constructs used the verified YFP-CESA6 plasmid as the template and were obtained by Q5 Site Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA) with primers listed in Supplement Table1 ...
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bioRxiv - Plant Biology 2020Quote: ... Libraries for next generation sequencing were generated using the NEBNext Ultra II gDNA prep kit with the NEBNext Multiplex Oligos for Illumina (New England Biolabs, Ipswich, U.S.A.). Prior to library generation ...
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bioRxiv - Cancer Biology 2021Quote: ... ChIP-seq libraries were prepared from 3-5ng ChIPed DNA using NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645S), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting polyA-selected RNA was used to prepare RNA-seq libraries using the NEBNext® Ultra(tm) II Directional RNA Library Prep Kit for Illumina® (cat. #E7600, New England Biolabs), NEBNext® Multiplex Oligos for Illumina® Dual Index Primers Set 1 (cat ...
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bioRxiv - Cancer Biology 2021Quote: ... A 3’ 3x FLAG tag was then inserted by site directed mutagenesis using a Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) and the primers PPARGC1A_FLAG_SDM_F and PPARGC1A_FLAG_SDM_R (see Supplementary Table 1) ...
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bioRxiv - Microbiology 2020Quote: Stranded RNA libraries were prepared from poly(A)-selected RNA using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced using a NextSeq 550 ...
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bioRxiv - Microbiology 2020Quote: ... The T7-3M-eGFP-5M minigenome was made by replacing the Renilla luciferase gene in the T7-3M-Luc-5M minigenome with an eGFP gene through assembly of PCR fragments using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs, MA, USA). A modified version of this minigenome ...
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bioRxiv - Cell Biology 2020Quote: ... as per the manufacturer’s protocol and reverse-transcribed to cDNA using a Protoscript first strand synthesis kit (New England Biolabs, cat. no. E6560S). Quantitative PCR was performed using SYBR Green (Biorad ...
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bioRxiv - Cell Biology 2020Quote: ... The targeting sequence was inserted into the sgRNA cassette of pDD162 using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs, Inc.), resulting in identical plasmids (pTS11 and pTS12).
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bioRxiv - Cell Biology 2020Quote: ... 3-4-points were induced in the miR-29a seed binding site using the Q5 Site Directed Mutagenesis Kit (NEB, Cat#E0554S). 20 ng of recombinant dual-luciferase plasmid and 6 pmol of either miR-29a mimic or scrambled control were mixed with 100 μL Opti-MEM containing 1 μL lipofectamine RNAiMax (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... using the LTP library preparation kit and employing NEBNext multiplex oligos for Illumina index primer pairs set 1 (New England Biolabs, Ipswich, Massachusetts). Sequencing was performed using a NextSeq 500/550 mid-output v2.5 300 cycle cartridge (Illumina ...
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bioRxiv - Genetics 2021Quote: ... 1 μg of total RNA per sample was used as input for library preparation using the NEBNext Ultra Directional RNA Library Prep kit for Illumina (NEB, cat. # E7420) with the PolyA selection with the NEBNext Poly(A ...
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bioRxiv - Plant Biology 2021Quote: ... 1.0 μg total RNA (0.1-0.5 μg for ovules) was used to generate mRNA-seq libraries using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, Cat# E7775). Sera-Mag Magnetic SpeedBeads (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA sample libraries were then prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified T7 PCR products were confirmed by sequencing and transcribed into dsRNA using the T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). The dsRNA produced was purified via ethanol precipitation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA-seq libraries were prepared from 150 ng total RNA using NEBNext® Ultra™ II Directional RNA library Prep Kit for Illumina (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA-seq libraries were prepared from 500 ng total RNA using the NEBNext® Ultra™ II directional RNA library Prep Kit for Illumina (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... barcoded libraries with a targeted insert size of 480 bp were prepared using the NEBNext Ultra II DNA Library Prep Kit (E7645L, New England Biolabs, Ipswich, MA) and sequenced on a HiSeq 2500 using a 2×125 bp paired-end protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Barcoded libraries with a mean insert size of 480 bp were prepared using the NEBNext Ultra II DNA Library Prep Kit (E7645L, New England Biolabs, Ipswich, MA) and sequenced on a HiSeq 2500 using a 2 x 125 bp paired-end protocol.
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bioRxiv - Neuroscience 2020Quote: ... was used in the preparation of sequencing libraries using the NEB Ultra II Directional RNA Library Prep Kit (NEB catalogue number E7760), following the polyA mRNA workflow ...
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bioRxiv - Developmental Biology 2020Quote: ... The sgRNAs were synthesised by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). Cas9 mRNA was prepared by in vitro transcription with the mMESSAGE mMACHINE T3 Transcription Kit (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... to a final volume of 25 μl for Illumina library preparation using the NEBNext ultra II DNA library prep kit for Illumina (New England Biolabs, UK; E7645). Following 7 cycles of PCR enrichment ...
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bioRxiv - Microbiology 2022Quote: ... The sequencing library for total RNA-sequencing was prepared using NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end ...
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bioRxiv - Plant Biology 2022Quote: ... Subsequent library preparation was performed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2022Quote: ... RAD-seq library preparation was performed with the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, Massachusetts, USA) with the PstI restriction enzyme ...
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bioRxiv - Genomics 2022Quote: Libraries were prepared using the NEBNext® Ultra DNA Library Preparation Kit for NovaSeq and NEBNext® Ultra II FS DNA Library Preparation Kit for MiSeq sequencing following the manufacturer’s recommendations (New England Biolabs, Ipswich, MA). For the validation experiments ...
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bioRxiv - Systems Biology 2022Quote: ... strand-specific total-transcriptome RNA sequencing libraries were prepared by incorporating dUTPs in the second-strand synthesis step with NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB). Finally ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNAs were subjected to sequencing library construction using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, E7645L), and were deep sequenced on a NextSeq 550 platform using 40nt/40nt pair-ended mode ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA synthesis was performed from 1 µg total RNA using the ProtoScript® First Strand cDNA Synthesis Kit (New England Biolabs, NEB) with the provided random primer mix according to the suppliers’ instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was used to remove rRNA from Escherichia coli total RNA and RNA-seq libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA E7760L). Libraries were recovered with AMPure XP beads A63880 (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was generated from 1 μg of total RNA using ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560).