Labshake search
Citations for New England Biolabs :
8701 - 8750 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... we used a similar protocol but library preparation was performed using the NEBNext Ultra DNA Library Prep Kit (New England BioLabs, ref. E7370) for Illumina following manufacturer’s specifications except for end repair ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... RNA sequencing library preparation used the NEBNext Ultra RNA Library Prep Kit for Illumina followed by manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1pmol of each fragment were then assembled for 1h at 50°C using NEBuilder HiFi DNA Assembly master mix from the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, ref E5520S). Reaction products were amplified in provided bacteria and purified using QIAprep Spin Miniprep Kit.
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bioRxiv - Molecular Biology 2024Quote: ... Enriched DNA was subjected to library construction using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (catalog no. E7645L, NEB), with the adapter ligation step performed at 50°C instead of 65°C ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, catalog #E6560S) with oligo-dT priming according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-Seq libraries are prepared by Integragen SA (Evry, France) with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina according to supplier recommendations (NEB). The capture is then performed on cDNA libraries with the Twist Human Core Exome +Custom IntegraGen Enrichment System according to supplier recommendations (Twist Bioscience) ...
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bioRxiv - Microbiology 2023Quote: HTS was performed on the three RNA extracts after a step of rRNA depletion with the rRNA depletion kit (NEB, Evry, France), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... AvcI RNA was synthesized by in vitro transcription using the T7-AvcI reverse complement DNA template and the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB™). Bio-11-UTP was included during the transcription reaction for Northern blot detection purposes ...
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bioRxiv - Molecular Biology 2023Quote: ... and pACG9-[EC1-10]) were generated through site-directed mutagenesis (NEB Q5 site-directed mutagenesis kit; New England Biolabs, Ipswich, MA, USA). Specifically ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA was amplified using an AMV first strand cDNA synthesis kit and was normalized to 50 ng/ul for each sample (New England Biolabs, Catalog # E6550). Sslac2 was amplified using specific detection primers (Sslac2 Det F and R ...
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bioRxiv - Physiology 2022Quote: RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... RNA sequencing library preparation used NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s recommendations (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then subjected to DNase treatment to remove any residual genomic DNA using Monarch RNA purification kit (NEB cat no. 2040S).
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bioRxiv - Microbiology 2022Quote: ... The sequencing library employed for total RNA sequencing was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA Magnetic Isolation Module and used to construct RNA-seq libraries with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to manufacturer’s protocol with slight modifications ...
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bioRxiv - Microbiology 2023Quote: ... One aliquot of RNA from each Leptospira serovar was processed with the addition of poly(A) tail using Escherichia coli poly(A) Polymerase Kit (New England Biolabs, Massachusetts, USA) following the protocols from the manufacturer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Whole-genome libraries were prepared using the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA) with modifications for subsequent target enrichment ...
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bioRxiv - Neuroscience 2023Quote: ... The RNA sequencing library was generated using NEBNext Ultra II Directional RNALibrary Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MS, USA). The sequencing library was validated on the Agilent TapeStation (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: ... The libraries for Illumina sequencing were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... End-repaired DNA was barcoded using the Native Barcoding Expansion kit (ONT, United Kingdom) and Blunt/TA Ligase Master Mix (New England Biolabs, United States). Barcoded samples were multiplexed and ligated to sequencing adaptor molecules in Adapter Mix II using the NEBNext Quick T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... gDNA from cell pellets was isolated using the Quick-DNA Midiprep Plus Kit (ZymoResearch, D4075) and genome-integrated sgRNA sequences were amplified by PCR using the Q5 Mastermix (New England Biolabs Next UltraII). i5 and i7 multiplexing barcodes were added in a second round of PCR ...
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bioRxiv - Microbiology 2022Quote: ... The sequencing library employed for total RNA sequencing was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was checked on a fragment analyzer and 50ng was used for library preparation with the NEBNext® Ultra™ II DNA Library Prep Kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Sonicated DNA was subjected to end-repair and adapter ligation using the NEBNext® Ultra™ II DNA Library Prep Kit (NEB) following the NEB protocol ...
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bioRxiv - Genomics 2023Quote: The RNA-Seq paired end sequencing libraries were prepared from the QC passed RNA samples using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB) as per manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The library was prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA) in combination with the NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Cell Biology 2023Quote: RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Synthetic Biology 2023Quote: ... The assembly reaction is conducted in a 25.0 μL assembly reaction mix composed of 1.0 μL NEBridge® Golden Gate Assembly Kit BsaI-HF® v2 (NEB® E1601), 2.5 μL T4 DNA ligase buffer 10X (NEB® B0202) ...
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bioRxiv - Plant Biology 2023Quote: ... Shotgun libraries for genome assembly were prepared using the NEBNext Ultra II DNA library preparation kit (New England Biolabs, Ipswich, MA, USA) and sequenced on Illumina HiSeq2000 (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μg RNA of each sample was used to generate cDNA libraries by NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England Biolabs). The qRT-PCR was performed using TB Green Premix Ex TaqTM (RR820A ...
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bioRxiv - Genomics 2023Quote: The strand-specific RNA sequencing library was prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... 150 ng of total RNA per sample was used to synthesize cDNA using LunaScript RT SuperMix kit (Cat# E3010L, NewEngland BioLabs, MA, USA) in a 20 µl reaction volume ...
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bioRxiv - Molecular Biology 2023Quote: 150 ng RNA was used to prepare libraries using the NEBNext Ultra II Directional mRNAseq kit with poly(A)+ purification module (NEB E7760, E7490) as described with modifications ...
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bioRxiv - Plant Biology 2023Quote: ... The enriched mRNAs were further taken for the library preparation using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (Catalog: E7775S, New England Biolabs). Sequence data was generated using Illumina HiSeq ...
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bioRxiv - Synthetic Biology 2023Quote: ... in a 20.0 μL reaction mix composed of 2.0 μL NEBridge® Golden Gate Assembly Kit BsaI-HF® v2 (NEB® E1601), 2.0 μL T4 DNA ligase buffer 10X (NEB® B0202 ...
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bioRxiv - Plant Biology 2023Quote: ... Library concentration was further quantified by qPCR using the NEBNext® Library Quant Kit for Illumina® (New England BioLabs® Inc.). Libraries were equimolarly pooled in batches of ∼15 (five libraries per age cohort ...
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bioRxiv - Genetics 2023Quote: ... unique Y-adapters containing UMI sequences and sample-specific P5 indexes were ligated to the DNA using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England BioLabs). Y-adapter-ligated DNA fragments were amplified by PCR1 using a TdTomato-specific forward primer and a reverse primer binding to the P5 sequence on Y-adapter ...
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bioRxiv - Genomics 2023Quote: ... The DNA library quality was assessed by a qPCR based on the NEBNext Library Quant Kit (New England Biolabs Inc., Ipswich, USA). Sequencing with standard Illumina primers was performed on a NextSeq 500/550 instrument (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina according to the manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA library was prepared using the NEBNext® Ultra II Directional mRNA Library Prep Kit (New England Biolabs, Frankfurt/Main, Germany). The quality of RNA and the library was determined by Nanodrop and Qubit (both Thermofisher) ...
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bioRxiv - Genomics 2023Quote: ... Libraries were constructed with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina according to manufacturer’s recommendations (NEB, cat. no. E7420) using 14 cycles of PCR ...
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bioRxiv - Genetics 2023Quote: ... were assembled in the pCFJ150 vector to create the pCT3.37 plasmid (NEBuilder® HiFi DNA Assembly Cloning Kit, New England BioLabs, cat#E5520). The protocol previously described for the generation of mosSCI transgenic lines (Frøkjær-Jensen et al. ...
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bioRxiv - Genomics 2023Quote: ... The PCR2 product pools were subjected to three steps of purification with the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L), Gel DNA Recover Kit (Zymoclean ...
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bioRxiv - Molecular Biology 2023Quote: ... and ChIP-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7546S, NEB) and sequenced on the Illumina HiSeq 2500 platform configured for 50-bp single-end reads.
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bioRxiv - Genomics 2023Quote: ... An additional USER treatment step was performed to the purified DNA by adding 2μl USER (included in all the NEB index primer kits) and incubating for 15 min at 37⍰ ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Library preparation was performed using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc., Ipswich, MA, USA). The quantity of the prepared DNA libraries was determined using a Qubit© 2.0 Fluorometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2023Quote: ... 1.2 μg of RNA was used as the starting material for the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA). Poly-A RNA was purified as per instructions and converted to adapter-ligated ...