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Citations for New England Biolabs :
7501 - 7550 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... donor plasmids were constructed by generating fragments using PCR from the indicated template with indicated primers in Data File 1 and assembled using NEBuilder HiFi DNA Assembly (NEB E5520S):
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bioRxiv - Neuroscience 2021Quote: ... total RNA (1 µg) was transcribed into cDNA using ProtoScript® II First Strand cDNA Synthesis Kit (Cat. No. E6560; New England BioLabs). Quantitative real-time PCR was performed with 20 ng of cDNA in a total reaction volume of 10 μL using Luna® Universal qPCR Master Mix (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA synthesis was performed from 1 µg total RNA using the ProtoScript® First Strand cDNA Synthesis Kit (New England Biolabs, NEB) with the provided random primer mix according to the suppliers’ instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was resuspended in 10x NEB CutSmart Buffer (8:1) and dephosphorylated by incubation with Quick calf intestinal phosphatase (CIP) (NEB, # M0525S) at 37 °C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: TCF20(1-1268)-mEGFP construct was generated from the TCF20-mEGFP plasmid using the Q5 Side-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The CIP-treated RNA was then PNK-treated with 1 μL of 10× T4 polynucleotide kinase buffer (New England Biolabs, B0201S), 2 μL of [γ-P32]ATP (PerkinElmer ...
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bioRxiv - Genetics 2021Quote: ... barcodes and barcoding adapters (PCR Barcoding Expansion 1-96, EXP-PBC096, Oxford Nanopore Technologies, UK) by PCR using Q5 polymerase mastermix (NEB, USA) for individual fish identification according to manufacturer’s protocol in a 25μl reaction (98°C for 3 min ...
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bioRxiv - Genomics 2021Quote: ... and a portion corresponding to 1 µg input RNA was converted to cDNA using the LunaScript RT SuperMix cDNA synthesis kit (NEB E3010). 1% of the cDNA was used for each qPCR reaction performed with the Luna Universal qPCR Mastermix (NEB M3003 ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were analyzed by SDS-PAGE and western blotting by using antibodies against MBP (1:1000, New England Biolabs, E8038S) or GST (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... fragments were generated by PCR from the indicated template with the indicated primers (Data File 1) and assembled using NEBuilder HiFi DNA Assembly (NEB E5520S). Plasmids were transformed into NEB competent cells (NEB C2987I) ...
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bioRxiv - Genomics 2021Quote: ... 2 μg genomic DNA from mESCs (both, with DSB induction and control without the induction) were digested using 1 μl 100 U/μL XbaI (NEB, #R0145T) and 4 μl 5 U/μL I-SceI (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting fragment was then checked by 1% agarose gel and purified with the Monarch® DNA Gel Extraction Kit (New England Biolabs). The purified fragment was precipitated by ethanol precipitation and resuspended in buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR was performed with primers specific for target genes (see Supplementary Table 1 for the list of primers) using the Luna universal One-Step RT-qPCR kit (NEB; E3005). Experiment was performed using the QuantStudio3 (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... a colony of each isolate was resuspended in 50 µL of sterile water then 1 µL of resuspended bacteria was used in a PCR reaction using Phusion® High-Fidelity DNA Polymerase (New England Biolabs) with forward (16s-8F ...
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bioRxiv - Systems Biology 2020Quote: ... the samples were then incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were then incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with an additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Microbiology 2021Quote: ... and then subcloned into the directly downstream of PreScission protease site pGEX-6P-1 (Cytiva) by Gibson Assembly system (New England Biolabs Inc.) to create pRN108 ...
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bioRxiv - Microbiology 2020Quote: ... A band at approximately 1 kb was cut out and DNA extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs, US) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the sgRNA cassette from lentiCRISPRv2 was removed by digestion with KpnI and EcoRI and the plasmid re-ligated using annealed repair oligo pair 1 (see Table S3) and Quick Ligation™ Kit (M2200L, NEB). Next ...
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bioRxiv - Plant Biology 2021Quote: ... deltoides xylem cDNA library (primers listed in Supplemental file 1) using Q5 High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) and cloned in a pENTR vector (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently purified by extraction from a 1% agarose gel using the Monarch DNA Gel Extraction Kit following manufacturer instructions (NEB # T1020S). The partially degenerate DNA oligonucleotide ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µg of GST-beads were incubated for 15 min on ice in GRB buffer in a 25 μl reaction volume with 1 µg of 100 bp DNA ladder (New England Biolabs # N3231S) or mononucleosome (Active motif # 81070) ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified inserts and expression vectors pGEX-4T 1 and pMALTMc5X were digested with BamHI/SalI (for pGEX-4T 1 constructs) and NotI/PstI (for pMALTMc5X constructs) restriction enzymes (New England Biolabs, UK) and were ligated over night at 16 °C using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... A reaction was carried out of the enzyme and its corresponding trisaccharide dissolved in 1X GlycoBuffer 1 (New England Biolabs, n.d.), deionized water ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μl of cells were used in a PCR reaction to obtain the gene for Sanger sequencing using Q5 (NEB, M0492). The PCR reaction was sent to Macrogen Europe B.V ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned into pGex6P-1 in-frame with an N-terminal 3C-cleavable GST tag using HiFi assembly (New England Biolabs, USA).
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bioRxiv - Cancer Biology 2020Quote: ... 25uM RibOxi RT primer was then annealed to the reaction before ligation of 5’ RNA linker using T4 RNA ligase I at room temperature for 1 hour and subsequent reverse-transcription using Protoscript II (NE Biolabs, M0368S). cDNA was then purified with AmpureXP beads with 1:1.8 ratio ...
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bioRxiv - Microbiology 2020Quote: We tested four high-fidelity and/or long-range DNA polymerases in duplicate to evaluate improvements of read length during the amplification step: 1) NEBNext (NEB M0541), 2 ...
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bioRxiv - Immunology 2022Quote: ... then the single-stranded oligos (1 ng) were PCR amplified for 10 cycles with Q5 High-Fidelity DNA Polymerase (NEB, M0491L) using Oligo_Amp_F and Oligo_Amp_R primers to generate double-stranded DNAs ...
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bioRxiv - Genetics 2022Quote: ... run at 50 V for 18 hours at 4°C in Tris-acetate buffer at pH 8.0 with comparison to Quick-Load 1 kb Extend DNA ladder (NEB, Cat# N3239S). Unless otherwise stated ...
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bioRxiv - Molecular Biology 2020Quote: ... 46 separate 60 μl PCR reactions were performed with 1 μg gDNA in each reaction using Q5® High-Fidelity DNA Polymerase (New England Biolabs). For the zeocin treated sample the total amount of genomic DNA harvested was used ...
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bioRxiv - Microbiology 2020Quote: ... We prepared a library with a fragment size of 300 nucleotides and used the 10 μM NEBNext Multiplex Oligos for Illumina (Set 1, NEB #E7335) lot 0091412 and the NEBNext multiplex Oligos for Illumina (Set 2 ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μL of 10x SSS buffer and 1 μL of SSS Enzyme (NEBNext mRNA Second Strand Synthesis Module, New England Biolabs, USA) were added to the 17 μL of the purified sample ...
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bioRxiv - Microbiology 2020Quote: ... and run on an agarose gel in 0.5X Tris-borate-EDTA buffer at 70V alongside 1 Kb ladder (New England BioLabs, Ipswich, MA) or GeneRule Express DNA Ladder (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... The PCR products were purified from a 1% agarose gel using the Monarch DNA Gel Extraction kit (New England Biolabs, USA) and send for Sanger sequencing provided by Edinburgh Genomics ...
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bioRxiv - Genetics 2020Quote: ... was annealed with 1ul of the reverse sgRNA (100 uM) using 1 ul 10X T4 ligation buffer (New England Biolabs, NEB), 1 ul T4 PNK (NEB) ...
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bioRxiv - Genomics 2020Quote: ... 1 μM of NEBNext Unique Dual Index Primers and 25 μl NEBNext Q5U Master Mix (M0597, New England Biolabs, Ipswich, MA) were added to the DNA and amplified as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 pg of lhx1a:DsRed/pI-SceI plasmid DNA was injected into 1-cell stage embryos along with the I-SceI restriction enzyme (NEB, R0694S). These injected embryos were raised to adulthood and screened for DsRed expression in known lhx1a expression domains and isolated a stable Tg(lhx1a:DsRed ...
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bioRxiv - Biochemistry 2021Quote: ... per 1g of wet cell weight and 1 Unit of 30 Units/μL RNase Inhibitor (from human placenta; New England Biolabs (NEB), No ...
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bioRxiv - Biochemistry 2021Quote: Nucleosome remodeling reactions (25 μL) were carried out in REA Buffer with 1 μL of PstI (NEB, at 100,000 U/mL), 2 mM ATP ...
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bioRxiv - Synthetic Biology 2021Quote: Expression units were assembled from cloned level 0 parts into level 1 destination vectors provided by the MoClo kit using 40fmol of each vector and 20 U of BsaI-HFv2 (NEB, R3733L) with remaining conditions as described above ...
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bioRxiv - Synthetic Biology 2021Quote: ... Vector and miRNA target inserts were ligated at 1:10 molar ratio using 400 U of T4 DNA Ligase (NEB, M0202) using provided Ligase buffer in a reaction volume of 20 µL over night at 16 °C ...
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bioRxiv - Cell Biology 2020Quote: Crushed lungs were homogenized using a mechanical homogenizer (Omni International, Waterbuy CT) or cultured cells were lysed in 1× lysis buffer (NEB, #9803S) supplemented with complete protease inhibitors (Roche ...
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bioRxiv - Genomics 2020Quote: ... The PCR was carried out with 1 μl of Lambda DNA (20 ng/μl; Catalog number: N3011S. Vendor: New England Biolabs Inc), 12.5 μl of KAPA 2X Master Mix ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA of 1×108 blood stage was extracted for conventional PCR amplification with a Q5® High-Fidelity DNA polymerase (New England Biolabs) from the interference inserts corresponding to the sense and anti-sense fragments of a portion of 429 bp AKT-like genomic sequence of T ...
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bioRxiv - Immunology 2021Quote: ... in YPD supplemented with 100 µg/ml ampicillin and 1% glucose (YPD-Amp-Glu) were infected with M13KO7 helper phage (New England Biolabs, USA) at 7×109 plaque forming unit (PFU ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5 kb and 500 bp DNA fragments were placed in two independent lines along with another line for 1 kb Ladder (NEB, N232). Once bands were clearly identifiable ...
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bioRxiv - Molecular Biology 2022Quote: ... Then the oligos went through PCR with KAPA HiFi HotStart Uracil+ Ready Mix and NEBNext Multiplex Oligos for Illumina Set 1 (NEB #E7600) as primers ...
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bioRxiv - Microbiology 2023Quote: ... RNA was diluted 1:50 for RT-qPCR which was conducted using the Luna Universal One-Step RT-qPCR kit (New England Biolabs E3005E) on a C1000 touch thermal Cycler / CFX384 Real-Time System (Biorad) ...
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bioRxiv - Microbiology 2022Quote: ... the CPER product was subject to post-PCR nick sealing for 30 min at 50°C and 30 min at 60°C in a 25 μL reaction containing 1 mM β-nicotinamide adenine dinucleotide (NAD+) (NEB) and 0.5 μL HiFi Taq DNA ligase (NEB) ...