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Citations for New England Biolabs :
7401 - 7434 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Mammalian cell lines were handled under sterile conditions using a laminar flow hood and the cells were regularly tested for mycoplasma contamination with a PCR-based mycoplasma detection kit (Minerva-Biolabs, cat.no. 11-1250). Subcultivation was performed every 2-4 days at a confluence of maximum 80 % using trypsin (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: Target amplicons were amplified by polymerase chain reaction (PCR) from the cDNA of slow swimmer and thecate cultures (Fig. S2A) with the Luna Universal qPCR Master Mix (NEB, Cat, No. M3003). The amplicons were run on a 1% (w/v ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA (transfected plasmid DNA) and plasmid DNA samples (that was used for transfection) with Phusion High-Fidelity PCR Master Mix (NEB Catalog no. M0531S) using primer set KC_BC (Table S4 ...
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bioRxiv - Genomics 2024Quote: The 493 gRNAs with associated 10N random barcodes were ordered as an IDT oPool and PCR amplified with Q5 High-Fidelity polymerase (NEB, Cat. No. M0491S) to make double stranded DNA ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was used as the template for IVT with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA, USA). Samples were then treated with DNase I and cleaned with the Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Escherichia coli and Myxcococcus xanthus were amplified from genomic DNA by PCR (Q5® High-Fidelity 2X Master Mix, New England Biolabs, USA-MA) and introduced into the pLIC expression vector55 by Gibson cloning (Gibson Assembly Master Mix ...
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bioRxiv - Genetics 2024Quote: ... An amplicon DNA library was made by designing amplicons to the coding regions of NR1H3 and PCR was undertaken using Q5® Hot Start High-Fidelity 2X Master Mix (Catalogue number M0494S, New England Biolabs® Inc) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... dilution of the universal adaptor and 13 cycles (or 16 cycles) of PCR per the respective masses with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Cat. No. E7760L), the NEBNext Poly(A ...
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bioRxiv - Microbiology 2020Quote: ... PCR protocols entailed an initial phase of 95° C for 3 min using Hot Start Taq polymerase (New England BioLabs Inc., Ipswich, MA, USA), followed by 35 cycles at 50°C and 52°C annealing temperatures for 16S and ITS amplicons respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were constructed from 2.5 ng of DNA and prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina with 9 PCR cycles (NEB #E7645S, New England Biolabs). Library quality was assessed by High Sensitivity DNA Assay on an Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Genetics 2021Quote: ... The PCR product was inserted to the PstI and BglII sites on the pWALIUM20 vector (Perkins 2015) by Gibson-assembly (NEB HiFi DNA Assembly Kit) to generate pWALEXA20 (White-AttB-LexAop for shRNA expression) ...
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bioRxiv - Plant Biology 2021Quote: ... the PCR reaction profile and program were setup as above with the following modifications: Q5 high-fidelity DNA polymerase (NEB, Frankfurt am Main, Germany) and 5x Q5 reaction buffer were used for PCR amplification ...
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bioRxiv - Genetics 2022Quote: ... and lysates were used as gDNA templates for PCR amplification with OneTaq® Quick-Load® 2X Master Mix with Standard Buffer (all materials from New England BioLabs, Ipswich, MA). Genotyping was performed with primers listed in Supplemental Table 6 with the following thermocycling conditions ...
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bioRxiv - Microbiology 2022Quote: ... The extracted DNA from water and soil underwent a further purification step using Monarch®PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA). DNA Library preparation was conducted using Native barcoding genomic DNA Kit SQK-LSK 109 combined with EXP-NBD104 (Oxford Nanopore Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Aliquots from these lysates were used for a PCR (with a Taq DNA polymerase with standard Taq buffer NEB or EconoTaq DNA polymerase Lucigen) with respective gene primers and an 18mer M13F-FAM fluorescent tagged primer (5’-TGTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Microbiology 2021Quote: ... The PCR amplification was carried out with Phusion High-Fidelity PCR Master Mix with HF Buffer in 50uL reactions with 20ng of genomic DNA (New England Biolabs Inc., Ipswich, MA, USA). Reactions were run in an Agilent SureCycler 8800 (Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were used as templates for in vitro transcription using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, New England Biolabs). Cas9 mRNA and gRNAs were then purified using RNAClean XP(Beckman Coulter ...
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bioRxiv - Bioengineering 2020Quote: ... 2–3 μl of genomic DNA was used as a template in a 50-μL PCR reaction with Q5® High-Fidelity 2X Master Mix (NEB, Ipswich, MA, USA). The following primers (Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... sequence of iRFP670 amplified from piRFP670-N1 vector by polymerase chain reaction (PCR) using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA) and primers encoding NcoI and XmaI digestion sites (Supplementary Table S1) ...
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bioRxiv - Bioengineering 2022Quote: ... 1ul of genomic DNA was used as a template in a 20ul PCR reaction using Q5 HotStart DNA polymerase (NEB, Cat. No./ID: M0493L). Each individual was genotyped for the presence of the y-chromosome (42) ...
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bioRxiv - Bioengineering 2022Quote: ... 1 µl of genomic DNA was used as a template in a 20 µl PCR reaction using Q5 HotStart DNA polymerase (NEB, Cat. No./ID: M0493L) and primers 1154A.S5 and 1154A.S7 amplifying genomic Fle sequences ...
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bioRxiv - Synthetic Biology 2023Quote: ... All PCR steps where the product was used in a cloning reaction were performed with Q5 High-Fidelity polymerase (NEB, Ipswich, MA, Cat#M0492S). After confirming the PCR product size with gel electrophoresis the PCR product was incubated with DpnI (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... Site directed mutagenesis of MLO2CT was performed by Gibson assembly (Gibson et al. 2009) based on suitable PCR fragments generated with Phusion® high-fidelity DNA polymerase (NEB GmbH, Frankfurt, Germany). The CAM2 coding sequence was inserted as an NcoI/XhoI DNA fragment into modified pET28a vector that lacks the N-terminal His6 tag (previously designated pETλHIS ...
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bioRxiv - Immunology 2023Quote: ... was generated by reverse transcriptase polymerase chain reaction (PCR) using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA). Transcript levels were measured by real time PCR using SYBR green on an ABI 7500 system ...
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bioRxiv - Bioengineering 2023Quote: ... All 5μl of the cell lysis were used for each Genomic PCR amplification using NEBNext® Ultra™ II Q5® Master Mix (NEB, M0544S). PCR program is 1 cycle ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µL of the PCR product was incubated with 2 U of EcoRI-HF® in 1X CutSmart® Buffer (New England BioLabs, Ipswich, MA, USA) at 37°C for 60 minutes ...
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bioRxiv - Genomics 2020Quote: ... The two groups of multiplex products from the gene-specific PCR were combined and carried over as templates to the second PCR which is performed using Phusion® High-Fidelity DNA Polymerase (New England Biolabs Inc., M0530L, Ipswich, MA) that barcodes each well/perturbation separately ...
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bioRxiv - Biophysics 2022Quote: Site-specific mutagenesis was performed with the Q5® Site-Directed Mutagenesis Kit (based on PCR-amplification) according to the manufacturer’s protocol (New England Biolabs GmbH, Frankfurt am Main, Germany). Oligonucleotides were ordered from metabion GmbH (Planegg/Steinkirchen ...
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bioRxiv - Systems Biology 2022Quote: ... custom-designed PCR primers that contain dual-indexed adapters were used to amplify the V3-V4 (806R/319F) region using NEBNext® Q5® Hot Start HiFi PCR Master Mix (New England Biolabs, Beverly, MA, USA). Samples were purified by two repeated 0.8X-ratio magnetic bead clean ups to remove all traces of primer ...
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bioRxiv - Bioengineering 2023Quote: ... plasmid pJC1-trpE (7749 bp) was amplified by PCR using oligonucleotides with degenerated (NNK) codons (NEB Q5 Site-Directed-Mutagenesis Kit, New England Biolabs, GmbH, Frankfurt am Main, Germany). PCRs were performed using the Q5 polymerase (Q5 Hot Start High-Fidelity ...
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bioRxiv - Zoology 2023Quote: Polymerase chain reaction (PCR) products were amplified in a total volume of 25µL with QuickLoad® Taq 2X Master Mix (New England Biolabs Inc., USA, catalogue number M0271L) and 0.2 µM primer for each pair ...
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bioRxiv - Plant Biology 2023Quote: ... The constructs M1 and M2 were used as templates to generate higher-order mutants by site-directed mutagenesis PCR (NEB, Q5 Site-Directed Mutagenesis Kit) using the primers listed in Table S2 ...
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bioRxiv - Molecular Biology 2022Quote: ... then quantified with the NEBNext® Library Quant Kit for Illumina® using qPCR on a StepOne Plus real-time PCR platform (New England Biolabs® Inc., MA, USA). Following qPCR ...
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bioRxiv - Plant Biology 2021Quote: ... These PCR products were then cloned into the pCAMBIA1301 vector (linearized by PstI and NcoI restriction enzymes, New England BioLabs, cat. no. R3140 and cat. no. R3193) using the In-fusion (Takara ...