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Citations for New England Biolabs :
7201 - 7250 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... RFP-histone H2B fusion protein (pRN3-C-term-RFP-Hist1h2bb) derived by in frame cloning of a high-fidelity PCR amplified cDNA (Phusion® DNA polymerase, New England BioLabs, M05305) encoding histone H2B (Hist1h2bb ...
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bioRxiv - Developmental Biology 2020Quote: The lft2 exon regions were amplified by PCR from genomic DNA isolated from tail fin clips of a surface fish and individual F61 PA-CF male and females using the Phusion High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA) and the primers listed in Table S3 ...
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bioRxiv - Molecular Biology 2020Quote: ... The mlrA gene was amplified from the MlrA-expression plasmid pASKA-MlrA (Table EV2) by PCR using Phusion High-Fidelity DNA polymerase (New England Biolabs, Tokyo, Japan) and the primer set Pure-Niwa-F and Pure-Niwa-R (Table EV3) ...
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bioRxiv - Genetics 2019Quote: ... Amplification of the coding sequence and 7 kb of surrounding non-repetitive sequence was performed with a nested PCR design to obtain specificity using NEB Longamp polymerase (New England Biolabs, Ipswich, MA). The region was divided into 2.5 kb and 4.5 kb regions ...
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bioRxiv - Molecular Biology 2019Quote: ... and then enriched via PCR amplification in a total volume of 50 μL containing 3 μL of NEB Next USER Enzyme (NEB, Ipswich, USA), 25 μL of NEB Next High-Fidelity PCR Master Mix (2× ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and quantified by qPCR on a Bio-Rad CFX Connect Real-Time PCR detection system with the NEBNext Library Quant Kit for Illumina (New England Biolabs, catalog # E7630L). The libraries were normalized to 10 nM ...
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bioRxiv - Microbiology 2019Quote: ... and 7 cycles of PCR to enrich for ligated product was done with NEBNext® Ultra™ DNA Library Prep Kit for Illumina (New England Biolabs). Libraries were quantitated with KAPA Library Quantification Kits for Next-Generation Sequencing (Roche Sequencing Solutions ...
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Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: ... Full length Rheb was PCR amplified from this vector and cloned into Smal-digested pEGFPC1 by Gibson Assembly (New England Biolabs, Ipswich, MA). The Q5 Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Biophysics 2019Quote: ... NG-Stop was created using the same inverse PCR product as NG-Scarlet and NG-Cherry via blunt end ligation using NEBs KLD Mix (New England Biolabs; Ipswich, MA). The mNeonGreen gene was obtained from the pmNeonGreen-NT plasmid (Allele Biotehcnology ...
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bioRxiv - Microbiology 2019Quote: Desired sequences were amplified from genomic DNA of Escherichia coli O157:H7 EDL933 in a PCR (Q5 polymerase, NEB, Ipswich, Massachusetts, USA) using different primer pairs ...
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bioRxiv - Genomics 2019Quote: ... PCR reactions for library preparation were performed by adding the following to the 10 μL DNA sample: 25 μL of Phusion High-fidelity PCR Master Mix (NEB catalog # M0531S), 1 μL of 5’ library index barcode ...
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bioRxiv - Microbiology 2019Quote: ... 10 (GSF1697) or 12 (GSF2248) PCR cycles were run using 8-nt barcoded oligos from NEBNext Multiplex Oligos barcode kit (NEB cat# 6609S). The libraries were purified with Agencourt AMPure XP beads ...
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bioRxiv - Genetics 2021Quote: ... Supernatant containing genomic DNA was used as a template for the PCR amplification of the 16S rRNA gene using Taq polymerase (New England Biolabs, Ipswich, MA) and the primers oMC98f (5’-GAAGAGTTTGATCATGGCTC-3’ ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Unique barcoded adaptor sequences were ligated to each sample of tagmented gDNA with 14 cycles of PCR using OneTaq 2x Master Mix (NEB, cat# M0482S), and all samples were pooled into a single multiplexed sequencing library ...
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bioRxiv - Cell Biology 2021Quote: ... Genomic DNA from cells collected at T0 and T18 was isolated as previously described [54] and sgRNA sequences amplified by PCR using Q5 Mastermix Next Ultra II (New England Biolabs, Cat# M5044L) with the following primers ...
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bioRxiv - Bioengineering 2021Quote: ... Cloning of PCR products and gBlocks in the pKSB- intermediate vector 43 was performed using NEBuilder® HiFi DNA Assembly (New England Biolabs, France) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the Irk1WT and Irk1V306A open reading frames were amplified from pENTR-Irk1WT or pENTR-Irk1V306A by PCR (Phusion high-fidelity DNA polymerase, New England Biolabs Cat #M0530). Primers pAc5-Irk1-F and pAc5-Irk1-R included additional sequence for subsequent Gibson assembly cloning into the multi-cistronic vector ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Twenty-four PCR reactions (24 x 50 μl) were performed with 1 U of Q5 high-fidelity polymerase (New England Biolabs®, M0491), 200 μM dNTPs ...
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bioRxiv - Plant Biology 2021Quote: ... The F1 fragment of eYGFPuv was assembled by cloning the gblocks and a PCR-amplified relevant fragment into an eYGFPuv vector 10 through NEBuilder HiFi DNA Assembly (New England BioLabs, Catalog #E5520S). Similarly ...
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bioRxiv - Microbiology 2020Quote: ... we separated the PCR products from the parental genomic DNA by gel electrophoresis and purified the PCR fragments using the Monarch DNA Gel Extraction Kit (New England Biolabs, Evry, Fr). The purified fragments were then mixed and fused together using assembly PCR carried out with the Q5 high-fidelity DNA polymerase ...
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bioRxiv - Neuroscience 2020Quote: ... Fragmentation and library construction of the resulting PCR products were performed using an NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the purified cDNA was PCR amplified and barcoded using ONT’s PCR Barcoding Expansion 1-12 (EXP-PBC001) or PCR Barcoding Expansion 1-96 (EXP-PBC096) kits with LongAmp Taq 2x Mastermix (NEB, Ipswich, MA).
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bioRxiv - Biophysics 2021Quote: ... a variable 89-bp hairpin stem capped by a (dT)4 tetraloop was ligated to two 1.5-kb double-stranded “handles” made by PCR amplification of sections of the pBR322 plasmid (New England Biolabs, Ipswitch, MA, USA). The left and right handles were respectively modified with 5’ biotin and digoxigenin to facilitate attachment to streptavidin- and anti-digoxigenin antibody-coated beads ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was carried out on 100 ng of gDNA per sample using Phusion High-Fidelity DNA Polymerase (New England Biolabs, Hitchin, UK) with an initial denaturation phase (98°C for 30 seconds) ...
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bioRxiv - Microbiology 2022Quote: ... colony PCR was conducted to amplify the 5’ IGR of the major T6 cluster using OneTaq DNA Polymerase (New England Biolabs – MA, USA). PCR products were confirmed with gel electrophoresis and Sanger sequencing by Eton Bioscience Inc ...
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bioRxiv - Genomics 2022Quote: ... and a PCR-free library was obtained with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs). Approximately 200 million of 150 bases pair-end reads were generated using the HiSeq X System (Illumina) ...
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bioRxiv - Immunology 2022Quote: ... The PCR enrichment of adaptor-ligated DNA was conducted with 7 cycles and NEBNext® Multiplex Oligos for Illumnina® (NEB, USA) for single end barcoding ...
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bioRxiv - Immunology 2022Quote: ... the tdTomato gene (codons optimized for expression in C. burnetii) was amplified by PCR with Q5 polymerase (New England Biolabs, Frankfurt, Germany) using primers a533 (5’-GATTTAAGAAGGAGATCTGCAGATGGTGTCAAAAGGAG-3’ ...
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bioRxiv - Plant Biology 2021Quote: The purified PCR reaction was then A-tailed with 15 units Klenow Fragment (3’ – 5’ exo-) (New England BioLabs; Ipswitch, MA, USA) in 1X NEB Buffer 2 (New England BioLabs ...
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bioRxiv - Developmental Biology 2021Quote: ... The template for probe synthesis was generated through two rounds of standard PCR method using OneTaq® 2x Master Mix (New England Biolabs, NEB): the first PCRs from cDNA used the gene-specific primers including the T7 linker sequence ...
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bioRxiv - Microbiology 2021Quote: ... The construct was amplified by 2-step PCR according to manufacturer recommendations using Q5 high fidelity polymerase (New England Biolabs, cat#M0494S) with primers 6469TSC and 6470TSC (Supplemental table 1) ...
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bioRxiv - Microbiology 2020Quote: ... and one fragment of about 320 bp of the 3’-terminal region by 3’ RACE were amplified using Phusion High-Fidelity PCR Kit (New England Biolabs, MA, USA) under the following conditions [98°C ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed in a two-step manner using a ProtoScript First Strand cDNA synthesis kit (New England Biolabs, Cat#E6300S) and iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and PCR products were digested with DpnI to eliminate plasmid template before setting up the assembly reaction (New England BioLabs, MA, USA). Finally ...
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bioRxiv - Immunology 2019Quote: ... Then sgRNA template for in vitro transcription (IVT) was prepared by PCR amplification of Phusion high fidelity DNA polymerase (NEB Biolabs, Ipwich, MA), the PCR mixture was cleaned up by PCR cleanup reaction (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... 3 μl from 100 μl of enzymatically converted and 1 μl from 15 μl of bisulfite-converted DNA were PCR amplified with Q5U polymerase (NEB, Ipswich, MA) and primers (Supplemental Table 3 ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR reactions were pooled and treated for 1 hr at 37°C with 25 U of Exonuclease I (NEB, Cat# M0293S) per 100 μL of pooled PCR reactions ...
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bioRxiv - Genetics 2022Quote: The full-length ccf-1 cDNA clone was generated through PCR using the Q5® High-Fidelity DNA polymerase (New England BioLabs, M0491L) and cloned into the pDEST32 vector containing the GAL4 DNA binding domain (DBD ...
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bioRxiv - Immunology 2022Quote: ... together with protease cleavage sites and restriction sites between the matrix and capsid domains of Gag of the full-length SIVMAC239 proviral DNA using PCR and the NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA) and used the same strategy as Hubner et al to generate the HIV Gag-iGFP 18 ...
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bioRxiv - Genomics 2022Quote: ... The mCherry cassette was cloned using G-block double-stranded DNA fragments synthesized by Integrated DNA Technologies (IDT) and PCR products generated using Q5 DNA polymerase (New England Biolabs, Cat#: M0492). The mCherry coding sequence was synthesized with silent mutations ablating potential splice donor and splice acceptor sites that could interfere with intended splicing of the intron ...
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bioRxiv - Cell Biology 2022Quote: ... mutated FN constructs were PCR amplified and ligated into BamH1+Xho1 cut NSPI vector using NEBuilder® HiFi DNA Assembly kit (BioLabs, #E5520).
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bioRxiv - Plant Biology 2019Quote: ... the sequence encoding PL1 (with no stop codon) was amplified using standard PCR reactions with a high fidelity Phusion Taq polymerase enzyme (New England Biolabs, Hitchin, UK) and appropriate templates ...
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bioRxiv - Molecular Biology 2020Quote: Thermocycler was used to denature and anneal 5 µl of PCR product as recommended by manufacturer (EnGen™ Mutation Detection Kit, NEB, E3321S). Thermocycler was adjusted for heteroduplex formation as following ...
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bioRxiv - Genetics 2020Quote: ... V416I and S438N were modified by site-directed mutagenesis PCR using Phusion®High-Fidelity DNA Polymerase (New England Biolabs, Massachusetts, USA). pGEMHE constructs were linearized using sbfI (New England Biolabs ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA substrates used for digestion reactions were either generated by PCR using Q5® High-Fidelity 2X Hot Start Master Mix (NEB #M0494), oligonucleotides produced by IDT ...
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bioRxiv - Genetics 2020Quote: ... This exonuclease-treated Klenow amplification was then used as a template in a standard 15-cycle PCR (Taq 2X Master Mix; New England Biolabs, MA. USA) using Illumina IDT-NXT primers carrying dual indexes (Supplemental Data 4).
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bioRxiv - Synthetic Biology 2020Quote: ... were PCR amplified from genomic DNA of yeast strain BY474129 and cloned together with the bidirectional inducible Gal1-10 promoter into the PCR amplified pRSII42B backbone with OZL14 and OZL15 via Gibson assembly30 using NEBuilder Kit (NEB, catalog E2621). MMR-DN mutant genes were subsequently generated via site-directed mutagenesis using either Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Synthetic Biology 2020Quote: PCR amplicons were randomly fragmented to an average size of 150-200 bp using NEBNext dsDNA Fragmentase (New England Biolabs, cat. M0348S), following manufacturer instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Homology arms were assembled into digested (SacI, XbaI) or PCR-amplified pLO3 backbone via Gibson Assembly (HiFi, NEB or In-fusion, Takara). C ...
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bioRxiv - Molecular Biology 2019Quote: ... IVT reactions were performed using the HiScribe T7 High Yield RNA Synthesis Kit using 300 ng of PCR product as template (NEB Cat. #E2040S). After an incubation for 16 hours at 37°C ...