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Citations for New England Biolabs :
7251 - 7300 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... PCR with Taq polymerase was performed in a 25-μl volume tube following the manufacture’s guidelines (New England Biolabs, Ipswich, MA, www.neb.com). PCR amplicons were visualized by agarose gel electrophoresis ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed in a final volume of 50 μL: 25 μL of Q5 polymerase master mix (New England Biolabs, MA, USA), 10 μL of GC enhancer buffer (New England Biolabs) ...
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bioRxiv - Genetics 2021Quote: ... PCR products with homologous DNA sequences (1 to 1.5 kb) flanking the deleted region were PCR-amplified with Phusion High-Fidelity DNA Polymerase (NEB, Ipswich MA, USA). These flanking PCR products were fused to two sides of either a NEO or NAT dominant selectable marker via overlap PCR ...
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bioRxiv - Genomics 2021Quote: ... thirty-two 50 µl PCR reactions were performed using 500 ng genomic DNA for each reaction and NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, M0541S). The purified libraries were sequenced on the NovaSeq 6000 with 150-bp paired-end sequencing ...
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bioRxiv - Physiology 2021Quote: ... Full-length cDNA sequences were obtained in 35 cycles of PCR reactions with Phusion DNA polymerase (New England Biolabs, Ipswich, USA; MO531L) and specific primers designed against the sequence of the phylogenetically characterized white seabass β-NHE obtained from the combined gill and RBC transcriptome (Integrated DNA Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... benthamiana IMPa-4 fragment were used for genomic PCR and followed by digestion with BamHI and XhoI (New England Biolabs, Ipswich, MA). The pTRV2 vector (ABRC ...
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bioRxiv - Developmental Biology 2020Quote: ... Inverse PCR was performed on the ligated genomic DNA using Q5 Hot Start High-Fidelity DNA Polymerase (M0493L, NEB, Ipswich, MA, USA). PCR products underwent primer walking Sanger sequencing (Sterky and Lundeberg ...
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bioRxiv - Cell Biology 2021Quote: ... YCplac111-Pah1-PrtA was linearized by inverse PCR and re-ligated using the NEBuilder HiFi DNA assembly mix (New England Biolabs, Ipswitch, Massachusetts) so that the Protein A sequence was replaced by a triple HA sequence ...
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bioRxiv - Cell Biology 2020Quote: ... primers 1 - 3) and then inserting the resulting PCR product into BamHI-digested pBMN-mCherry using Gibson assembly (New England Biolabs, Ipswich, MA). The resulting pBMN-ARHGAP36-mCherry vectors with XhoI and SacII restriction sites were subsequently used in all experiments described herein.
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... Gel purified PCR product soo-XbaI-Gateway-XbaI-soo was then cloned into pTGA linearized by XbaI digestion (New England Biolabs, cat#R0145S) using SoSoo mix reagent (TreliefTM SoSoo Cloning Kit ...
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bioRxiv - Immunology 2021Quote: ... was isolated from cDNA of devil peripheral blood mononuclear cells (PBMCs) by PCR using Q5® Hotstart High-Fidelity 2X Master Mix (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Immunology 2020Quote: The viral nucleic acids detected by PCR of the LTR-gag region were digested with the restriction endonuclease ScaI (New England BioLabs, Beverly, MA), which cuts within the amplicon of HIV-2287 but not SIVmne ...
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bioRxiv - Immunology 2020Quote: ... The heavy and light chain PCR products were cloned in frame with seamless cloning using the NEBuilder® HiFi DNA Assembly Master Mix (NEB, UK) after linearizing the vectors with KpnI (5’ ...
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bioRxiv - Immunology 2021Quote: ... 62 was modified to introduce IL6 secretion signal (IL6ss) upstream of Myc-Nluc using PCR-based approach with NEBuilder DNA assembly (New England Biolabs, Ipswich, MA). Lentiviral helper plasmids expressing HIV Gag-Pol ...
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bioRxiv - Immunology 2021Quote: ... The concentration of libraries was determined by quantitative PCR using a NEBNext library Quant kit for Illumina (New England BioLabs, Ipswich MA). The size of libraries and amplicon profiles were determined by the Fragment Analyzer System (Agilent Technologies ...
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bioRxiv - Genomics 2021Quote: ... DNA was diluted ten-fold and used as template for the second round of PCR where Illumina adaptors for sequencing were added using the NEBNext® Multiplex Oligos Illumina dual index kit (NEB, UK) with 10 cycles of 10 seconds at 98°C ...
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bioRxiv - Biochemistry 2022Quote: ... Site-directed mutant libraries were constructed via overlap extension PCR and standard ligation using T4 DNA ligase (New England Biolabs, Beverly, MA). Oligonucleotide primers carrying NNB base triplets were purchased from Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2022Quote: Amplicons of tagged-Cdep were isolated by gel extraction after PCR amplification using Phusion high fidelity polymerase (New England Biolabs, catalog #E0553L). The amplicons were then cloned into the pJFRC7 5x UAS vector (ref ...
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bioRxiv - Genomics 2022Quote: The bead-bound ligation products were amplified 8-13 cycles by PCR (Supplementary Information Table 5 for cycle recommendations according to starting cell number) using Phusion high-fidelity PCR master mix with HF buffer (New England Biolabs cat. #M0531L)
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bioRxiv - Immunology 2022Quote: ... and unc-54 3’utr pieces were Gibson assembled using the NEBuilder assembly kit and the assembled product was then PCR-amplified with Phusion DNA polymerase (New England Biolabs, Ipswich, MA). The PCR-amplified assembly was A-tailed for 30 min at 70 °C ...
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bioRxiv - Genetics 2022Quote: ... The reaction mixture was composed of a PCR-ready mix (using NEBNext® Ultra™ II Q5® Master Mix, NEB, M0544L), a cell lysis product ...
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bioRxiv - Neuroscience 2022Quote: ... the 181-bp and 376-bp amplicon products (Figure 1-figure supplement 1A) were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) and sent for Sanger DNA sequencing at the OHSU Vollum Sequence Core (Portland ...
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bioRxiv - Synthetic Biology 2022Quote: ... plasmid (Monarch® PCR mini-prep kit) and gel extraction kits (Monarch® DNA gel extraction kit) were also obtained from NEB and used according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... DNA was subjected to qRT-PCR analysis using the Luna Universal qPCR Master Mix Protocol (New England Biolabs, Frankfurt am Main, Germany) and a CFX96 Real-Time System ...
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bioRxiv - Neuroscience 2023Quote: ... Digested backbone and PCR amplified fragments were joined via seamless cloning (NEBuilder® HiFi DNA Assembly Master Mix, NEB, Cat. No. E2621S). Assembled plasmid was Sanger-sequenced to ensure correct assembly and reading frame ...
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bioRxiv - Synthetic Biology 2022Quote: ... vectors were from Park and Kim.11 The AtADT2 sequence and pHyo182 backbone were PCR-amplified and Gibson-assembled (NEB, Ipswich, MA). The S222N mutant of AtADT212 was recreated by site-directed mutagenesis (Q5® Kit ...
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bioRxiv - Cell Biology 2022Quote: ... PRR14 1-212 PCR products were sub-cloned into the expression vector pCMV6-AN-mGFP using T7 ligase (New England Biolabs, cat# M0318S) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... Microexon 4 sequence (nucleotides 1258-1281) was deleted by PCR on the pBSK-nCPEB4 plasmid using Gibson Assembly® Master Mix (New England Biolabs, E2611S), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids were constructed by assembling DNA fragments amplified by PCR into restriction endonuclease-digested vectors using NEB Hifi Builder (NewEngland BioLabs; cat.no: E2621L). The plasmids were confirmed with restriction digestion and sequencing.
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bioRxiv - Cell Biology 2024Quote: ... was fused to the cDNA sequence of mScarlet-I by PCR and subcloned into pMNATZA1 using Gibson assembly with NEBuilder HiFi DNA Assembly (New England Biolabs, Ipswich, MA). The spPKA-KTR1.0 is stably expressed under the Padh1 promoter from the Z locus ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A PCR master mix was employed with final concentrations of Standard ThermoPol buffer Mg-free (1x) (New England Biolabs, Ipswich MA, USA), dNTPs (0.2 mM each ...
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bioRxiv - Developmental Biology 2024Quote: ... RUW regions were amplified from genomic DNA of transfected populations via PCR with the high fidelity Q5 polymerase (New England Biolabs, Cat. #M0491S), with an annealing temperature of 62 degrees and performing 35 cycles ...
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bioRxiv - Neuroscience 2023Quote: ... samples were split into two separate reaction tubes containing 8 μL of PCR product + 1 μL 10X NEB® Buffer #2 (New England Biolabs®). These were incubated in a thermocycler with an initial denaturation of 95 °C for 5 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... The resulting fragment was subjected to error-prone PCR under the following conditions: 5 U of Taq DNA polymerase (New England Biolabs, MA, USA), 200 μM of each deoxynucleoside triphosphate ...
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bioRxiv - Microbiology 2023Quote: ... 9 DNA fragments encoding the partial genome of SARS-CoV-2 were prepared by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Cat# M0491S). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Molecular Biology 2023Quote: ... gDNA from cell pellets was isolated using the Quick-DNA Midiprep Plus Kit (ZymoResearch, D4075) and genome-integrated sgRNA sequences were amplified by PCR using the Q5 Mastermix (New England Biolabs Next UltraII). i5 and i7 multiplexing barcodes were added in a second round of PCR ...
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bioRxiv - Immunology 2023Quote: ... The multiplex products were used as templates to the second PCR using Phusion High-Fidelity DNA Polymerase (New England Biolabs Inc., M0530L) that barcodes each well/perturbation separately ...
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bioRxiv - Bioengineering 2023Quote: ... from the AAV genomes following the fourth round of selection by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs, Ipswich, MA) and 21 cycles of PCR ...
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bioRxiv - Immunology 2023Quote: ... using primers that overlapped with pLenti-EF1-Blast at the xho1 site on the 5’ end and overlapped with the 5’-end of the RNase L coding sequence on the 3’-end (Supplementary table 1) and NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs: M0541S). RNase L coding sequence was amplified using a 3’-end primer that overlapped with pLenti-EF1-Blast at the xba1 site ...
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bioRxiv - Immunology 2023Quote: ... Real-time quantitative PCR was run on the Eppendorf Mastercycler (SybrGreen) using the LUNA Universal qPCR Master Mix (New England Biolabs, Catalog # M3003). Primers used for real-time qPCR can be found in Supplementary Data 5.
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bioRxiv - Immunology 2023Quote: ... Read 1 and Read 2 sequencing primers (sequences listed in Table S2) were added PCR fragments by T/A ligation using NEBNext Ultra II Ligation Module (E7595, New England Biolabs, Ipswich, MA). Spacers and minimal promoter were added to sequencing primer-ligated enhancers using the primers 5BC-AG-f02 and 5BC-AG-r02 (Table S2) ...
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bioRxiv - Genomics 2023Quote: ... The PCR2 product pools were subjected to three steps of purification with the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L), Gel DNA Recover Kit (Zymoclean ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We size-selected these libraries using a Pippin Prep (Sage Science, Beverly MA) and then amplified and indexed them via PCR (Phusion, New England Biolabs, Ipswich MA). At each stage ...
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bioRxiv - Molecular Biology 2023Quote: ... and the integrated sgRNA sequences were amplified and barcoded by two-step PCR using NEBNext Ultra II Q5 Master Mix (New England Biolabs, cat # M5044). The barcoded sgRNA samples were sequenced on an Illumina NextSeq500 to quantitate representation of each sgRNA in the Ub/Ubl pathway inhibitor-treated or non-treated control samples ...
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bioRxiv - Microbiology 2023Quote: ... the Strep-tag® II sequence was inserted at the C-terminus via Phusion® High-Fidelity DNA Polymerase mutagenesis PCR (New England Biolabs) using the primers pYS14_GA_F/ 5’ phosphorylated flaakstrp14R to amplify the whole plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: BigBlocks were PCR-amplified with dedicated primer pairs in a 25.0 μL PCR reaction mix composed of 0.25 μL Q5® Hot Start High-Fidelity DNA Polymerase 2 U/μL (NEB® M0493), 5.0 μL Q5® 5X buffer (NEB® B9027) ...
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bioRxiv - Cell Biology 2023Quote: ... Hbx insert was PCR amplified from pcDNA3-HBV(Gao et al., 2004) with Phusion DNA polymerase (New England Biolabs Cat. No. M0530S) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... purified and subjected to ligation with the digested and gel-purified PCR product utilizing T4 DNA ligase (New England Biolabs, Cat. # M0202S).
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bioRxiv - Genomics 2023Quote: ... sgRNA2 and sgRNA3 from the 4sgRNA library were amplified (595 bp) by PCR using NEB Q5 DNA polymerase (NEB, Ipswitch MA, USA) with a universal P7 primer and individual P5 primer ...
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bioRxiv - Biochemistry 2023Quote: ... and then the two PCR products were co-transformed into yeast along with pCB05 digested with BstXI (New England Biolabs [NEB], Catalog # R0113S) and XhoI (NEB ...