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Citations for New England Biolabs :
6851 - 6900 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The partial AtVPS13M1 gene was amplified by PCR in 45 cycles using LongAmp Hot Start Taq 2X Master Mix (New England Biolabs), from 30/40ng of cDNA per sample in reaction volume of 100µl ...
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bioRxiv - Cell Biology 2024Quote: EGFP was PCR-amplified from pCIneo-FlucDM-EGFP119 and subcloned in pcDNA4/TO using the restriction enzymes XhoI and XbaI (NEB). Nudfa1 and Ndufa2 was amplified from HEK293T cDNA and cloned into pcDNA4/TO-EGFP using KpnI and XhoI (NEB) ...
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bioRxiv - Genomics 2024Quote: ... All rmp loci were amplified from genomic DNA by PCR and then cloned into pMWO-07829 by Gibson assembly (NEB). The rmp expression vectors were then transformed into KPPR1S Δrmp ...
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bioRxiv - Microbiology 2024Quote: ... One micro litter of circularized cDNA was subjected to PCR in a 20 μL reaction mixture composed of 20 U/mL Phusion polymerase (NEB), 1x Phusion GC buffer (NEB) ...
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bioRxiv - Genomics 2024Quote: ... and a total of 50 ng was used as a template for PCR amplifications encompassing ebony exons e1 and e2 with OneTaq HS Quick-Load Master Mix with Standard Buffer (NEB) as described before.
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bioRxiv - Microbiology 2024Quote: ... The RT reaction was diluted 2-fold with nuclease free water and 1 μl of the diluted mix was subjected to 5’ RACE PCR using Q5 polymerase (NEB) with a touchdown PCR protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Both libraries were assembled through inverse PCR (iPCR) on the pET23-KOD-Exo-(Supplementary Information S1 Table S2) plasmid in Q5 High-Fidelity DNA Polymerase (NEB) reactions of 28 cycles following the manufacturer’s standard protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and pCAH-mScarlet-C3 using KOD One PCR Master Mix (#KMM-101X5, TOYOBO) and NEBuilder HiFi DNA Assembly Master Mix (#E2621L, New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: Each of the selected eight promoters were amplified by PCR and individually inserted by Gibson assembly (#E2611S; New England Biolabs) into the reporter vector ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples with expected deletion (∼400bp) in the PCR screen were then treated with Shrimp Alkaline Phosphatase and Exonuclease I (New England Biolabs) and sent out for Sanger sequencing (Eurofins Genomics).
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bioRxiv - Cell Biology 2024Quote: ... The vector backbone was also PCR-amplified and the three fragments were assembled with NEBuilder HiFi DNA assembly kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... 1998) was amplified by PCR and then inserted into the pBS vector using NEBuilder HiFi DNA Assembly Master Mix (NEB) to generate pBS-UASp ...
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bioRxiv - Genomics 2024Quote: ... PCR amplified and then pooled into equimolar amounts and further quantified using the NEBNext Library Quant Kit (New England Biolabs). The pooled library was subjected to 50 bp paired-end sequencing on the Illumina NextSeq 2000 platform.
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... of the Oprl1 gene were amplified from a C57BL/6 BAC clone by PCR using Q5 Polymerase (New England Biolabs) and cloned into polylinkers of a targeting construct that contained IRES-mnCre:GFP ...
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bioRxiv - Biochemistry 2024Quote: ... Site-directed mutagenesis was performed using standard PCR-based mutagenic procedures with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR products were assembled using 75 ng of the backbone PCR DNA and 12.5 ng of the PCR amplified library in a 15 μL Gibson assembly reaction using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) at 50 °C for 1 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... Shipston and was N-terminally attached to an RFP (BKCa-DECRFP) using KpnI and BamHI restriction sites after PCR amplification (NEB Q5 High-Fidelity DNA-Polymerase ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The 1392-bp region comprising the E9 region was PCR amplified from genomic DNA and cloned upstream of the DPYD promoter using KpnI and SacI sites (New England Biolabs). A vector containing the A allele of rs4294451 within E9 was confirmed by Sanger sequencing ...
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bioRxiv - Biophysics 2024Quote: ... A 30 nt 3′ overhang was created by treating the purified PCR product with USER enzyme mix (NEB, Cat# M5505S) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Shipston and was N-terminally attached to an RFP (BKCa-DECRFP) using KpnI and BamHI restriction sites after PCR amplification (NEB Q5 High-Fidelity DNA-Polymerase, New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2024Quote: ... the entire dotB sequence with 500 bp flanked regions was PCR amplified from a SP-1303 genomic DNA extract using a high-fidelity polymerase (Phusion, NEB). Purified amplicons were cloned at SalI and SphI sites (NEB enzymes ...
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bioRxiv - Microbiology 2024Quote: ... Ligation with gentamycin cassette PCR amplified from the plasmid pACEBac1 (GenevaBiotech) was performed O/N at 16 °C using T4 DNA Ligase (NEB). Competent E ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR products were cloned via Gibson assembly60 in pBEL1784 previously digested with EcoRI-HF and NotI-HF (New England Biolabs). In addition ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µl of complementary DNA (cDNA) from the MaP reverse transcription reaction was used as template for PCR with Q5 hot-start polymerase (NEB) according to manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... DNA libraries were prepared from cDNA using a two-step PCR process (Q5 Hot Start High-Fidelity DNA Polymerase, New England Biolabs). PCR 1 used Step-1 Forward primer and Step-1 Reverse primer ...
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bioRxiv - Microbiology 2024Quote: ... a commercially available PCR Master mix was used (LongAmp® Hot Start Taq 2X Master Mix, New England BioLabs, USA). For each run ...
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bioRxiv - Microbiology 2024Quote: ... The recombinant plasmids were created by PCR amplifying two (ops) or three (HP-ops) DNA segments using Phusion polymerase (NEB) with ΔmpiM44 as template with the primers listed in Table S2 ...
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bioRxiv - Plant Biology 2024Quote: ... Primers for PCR fragments bearing 20 bp overlapping to the flanking region were produced with Phusion High-Fidelity DNA Polymerase (NEB) and 40-mer primers ...
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bioRxiv - Synthetic Biology 2024Quote: ... Mutants were generated through inverse PCR (iPCR) on the pET23-KOD-Exo-(Table S2) plasmid in Q5 High-Fidelity DNA Polymerase (NEB) reactions of 30 cycles following the manufacturer’s standard protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... 30 uL of the purified PCR amplicons were then digested and ligated in a 50 uL one-pot reaction containing 1x T4 ligase buffer (NEB), 20U DpnI (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: 50-100 ng of purified PCR product was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: 175-225 ng of purified PCR product was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... The purified PCR product was assembled with an NdeI-HindIII-cut pIJ10257 backbone using a 2x Gibson master mix (NEB). The resulting plasmids were verified by Sanger sequencing (Genewiz ...
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bioRxiv - Microbiology 2024Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs) and the primers listed in Suppl ...
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bioRxiv - Microbiology 2024Quote: ... and held at 72°C for 15 minutes before purification by PCR and DNA cleanup kit (New England Biolabs, T1030S) according to manufacturer instructions with the following modifications ...
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bioRxiv - Microbiology 2024Quote: The sgRNA-containing region of the lentivirus in the sample DNA was PCR amplified using NEBNext Ultra II Q5 Master Mix as per manufacturers’ instructions (New England Biolabs), and with primers (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Purified PCR products were cloned into the PmeI site of the pMSR0 vector using NEBuilder HiFi DNA Assembly (New England Biolabs). The resulting plasmid was transformed into E ...
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bioRxiv - Molecular Biology 2024Quote: ... Deletion constructs were made by PCR amplification of the appropriate regions and cloned into the Cilantro 2 vector using Gibson cloning (New England Biolabs). Lentiviral particles carrying the respective constructs in the Cilantro 2 vector were produced and used to transduce MOLM-13 cells as described above ...
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bioRxiv - Molecular Biology 2024Quote: ... Ligation products were purified by 1.1×DNA FragSelect XP Magnetic Beads and amplified by 12-15 cycles of PCR with NEBNext Ultra II Q5 Master Mix and NEBNext Multiplex Oligos for Illumina (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: PCR products amplifying the C-terminal region of CBP in both edited and unedited cells were cleaned up using the Monarch PCR cleanup kit (NEB). DNA concentrations were measured using the Qubit dsDNA BR kit (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates from HEK293T transfections performed on two separate days were used as template for genomic PCR using the Q5 polymerase (NEB) and visualized on a 2% agarose gel.
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was cloned into the pBS backbone by restriction digest and ligation using T4 DNA ligase (NEB, M0202S). For the construct with a structured 5’UTR (5’UTR-Hairpin) ...
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bioRxiv - Microbiology 2024Quote: ... was amplified using primers OVL7966 and OVL6481 and the PCR product was excised from a 2% agarose gel using the Monarch DNA cleanup and gel extraction kit (NEB). One-step Golden Gate Assembly (GGA ...
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bioRxiv - Molecular Biology 2024Quote: ... We subjected 100 ng of DNA to a first round of PCR (25-28 cycles, Q5 hot start high-fidelity DNA polymerase (New England Biolabs)) to amplify the locus of interest and attach common overhangs ...
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bioRxiv - Synthetic Biology 2024Quote: ... The addition of an NLS or DST to level 1 and level 2 integrase constructs was performed using PCR-mediated site-directed mutagenesis (NEB Q5 Site-Directed Mutagenesis ...
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bioRxiv - Biophysics 2020Quote: ... a point mutation was introduced in pET15b-TP 53 by PCR and Gibson Assembly with two restriction enzymes (NdeI (New England Biolabs, R0111) and BamHI (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... reaction with 2 to 4 µg of genomic DNA in a 50 µl reaction using the Next High-Fidelity 2x PCR Master Mix (NEB, M0541) (according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The presence of the PCR products was confirmed by gel electrophoresis and the products were then purified by Monarch® PCR & DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Immunology 2021Quote: ... was isolated from cDNA of devil peripheral blood mononuclear cells (PBMCs) by PCR using Q5® Hotstart High-Fidelity 2X Master Mix (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Genetics 2021Quote: ... We then amplified 10 uL of cDNA in 100 uL PCR reactions using NEBNext Ultra II Q5 Master Mix (NEB, M0544S) and Lib_Seq_eGFP_F2 and Lib_Hand primers for either 8 or 3 cycles (MPRA 1 and 2 respectively) ...