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Citations for New England Biolabs :
6251 - 6300 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... mRNA enrichment and library preparation was performed using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II RNA Library Prep kit (NEB). Sequencing was done using the Illumina NextSeq500 to obtain >20 million 75bp single-end or 37bp paired-end reads per sample or at Genewiz (HiSeq ...
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bioRxiv - Microbiology 2022Quote: ... 1μg of input RNA was used for RNA library preparation using the NEB Next Ultra II RNA Library Prep kit for Illumina (E7775L; NEB). Prepared libraries were quantified using real time PCR and bioanalyzer ...
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bioRxiv - Genetics 2022Quote: ... We prepared the libraries with the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Small RNA-seq libraries were generated from the small RNA fraction by TCAG using NEBNext Small RNA Library Kit (New England BioLabs) according to the manufacturer’s protocol in two batches ...
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bioRxiv - Cancer Biology 2022Quote: ... The L325R mutation was introduced to the EGFP-talin1 head construct using the Q5-site directed mutagenesis kit (NEB, E0554).
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bioRxiv - Biochemistry 2022Quote: The mutant constructs used in this paper were prepared by point directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB). Primers were designed and annealing temperatures determined using the NEBase Changer tool ...
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bioRxiv - Developmental Biology 2022Quote: ... PE3-EGFP-2-Fw and PE3-EGFP-2-Rv) were annealed and inserted into the BsmBI sites of BPK1520 using a Quick Ligation Kit (New England BioLabs). PuroR cDNA was amplified from PX459 with primers (PuroR-Fw ...
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bioRxiv - Developmental Biology 2022Quote: ... and integrated into a MluI-NheI site of pT2A-CAGGS (Urasaki et al., 2006) using a Gibson Assembly Kit (New England BioLabs). To obtain pT2A-CAGGS-PEmax-ires-ZsGreen1 ...
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bioRxiv - Biochemistry 2022Quote: ... the serine residues S240 and S250 in Dsn1 were mutated to aspartic acid using the Q5 site-directed mutagenesis kit (New England Biolabs) as described previously 19,20 ...
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bioRxiv - Biochemistry 2022Quote: We assembled mutant libraries by combining the linearized sensor backbone with each oligo subpool at a molar ratio of 1:5 using Golden Gate Assembly Kit (New England Biolabs; 37 °C for 5 min and 60 °C for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... Site-directed mutagenesis to create the EcGhrAW45F variant was performed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) using the mutagenic primers 5’-TGCTTTAGTCTTTCATCCTCCTG-3’ (forward ...
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bioRxiv - Genetics 2022Quote: ... This pooled mixture was run on a 2% agarose gel and we extracted and purified DNA in the 400 bp to 600 bp region using the Monarch Gel Extraction Kit (NEB) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... We used this synthetic HIS3 fragment and BsmBI-v2/HpaI-digested PFA0055 to create plasmid PFA0227 by isothermal assembly cloning using the HiFi Assembly Cloning Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... All samples were processed in a single batch and the isolated mRNA from each sample was used to prepare RNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... We then combined SwaI digested BFA0190 and the components of each TFT reporter and used the NEB HiFi Assembly Kit (NEB) to produce each TFT plasmid ...
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bioRxiv - Genomics 2022Quote: Illumina libraries were constructed from 100 ng of amplified cDNA libraries using the NEBNext Ultra II FS library prep kit for Illumina (New England Biolabs) with some modifications ...
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bioRxiv - Biochemistry 2022Quote: ... CXCR7 phosphorylation site mutants were generated by site-directed mutagenesis using Q5 Site-Directed Mutagenesis Kit (NEB, Cat. no. E0554S). For the NanoBiT assay ...
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bioRxiv - Biochemistry 2022Quote: ... The library was cloned into a pENTR1A plasmid backbone with a C-terminal YFP HA reporter tag using Gibson Assembly (HiFi DNA Assembly Cloning Kit, New England Biolabs, (NEB), Ipswich ...
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bioRxiv - Biochemistry 2022Quote: ... Single and multiple point mutation modifications to the pcDNA C12 construct were generated through the Q5 Site-directed Mutagenesis kit (NEB) or purchased as a minigene from Integrated DNA Technologies (IDT ...
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bioRxiv - Genetics 2022Quote: ... and extracted and purified DNA in the 400 bp to 600 bp region using the Monarch Gel Extraction Kit (NEB) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Mutation of the verified 7-mer binding site of the 3’-UTR of Zfp36l1 was performed using the Q5-site directed mutagenesis kit (New England BioLabs) using the following primers ...
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bioRxiv - Immunology 2022Quote: ... In vitro transcription (IVT) was performed at 37°C for 13-16h with the HiScribe T7 RNA Polymerase kit (NEB). The remaining DNA was digested by Turbo DNase I (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... and subsequently modifying the plasmid to add the HA-tag coding sequence using the Q5 Site-Directed Mutagenesis kit (NEB) or the mEos3.2 coding sequence using InFusion (Clontech) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Extracted gDNA was prepared for sequencing using a modified protocol63 for the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) at ½ of the recommended reaction volume ...
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bioRxiv - Physiology 2022Quote: ... Stranded cDNA libraries were created with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). The 30 fish were sequenced for 100 base pair reads on one lane of a NovaSeq 6000 (Illumina) ...
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bioRxiv - Molecular Biology 2022Quote: ... Concentrations were measured by Qubit RNA HS assay kit and 750 ng of Dnase-treated RNA was used for rRNA depletion by NEB Next rRNA depletion kit (Human/Mouse/Rat) ...
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bioRxiv - Microbiology 2022Quote: ... for removal of contaminating genomic DNA before it was purified with Monarch® RNA cleanup kit (New England Biolabs, USA). The concentration of RNA and potential DNA contamination were quantified using Qubit™ RNA BR and Qubit™ dsDNA HS assay kits ...
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bioRxiv - Molecular Biology 2022Quote: ... fluorophores were prepared by PCR amplification from the pTriEx-MLucV plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). The proteins were expressed and purified in a similar way to MLucV ...
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bioRxiv - Molecular Biology 2022Quote: ... The cDNA library was constructed following the manufacturer’s instructions of NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2022Quote: ... David Sibley) into the sgRNA targeting the first exon of TgPRL gene by using Q5 Site-Directed Mutagenesis Kit (NEB). A DNA fragment containing the DHFR selection cassette flanked by short homology to the upstream and downstream of the guide RNA target site was amplified using pJET-DHFR (a gift from Dr ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription of 50 ng total RNA was performed using Protoscript II First Strand cDNA Synthesis Kit with oligo(dT)23 primers (NEB). cDNA was undiluted ...
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bioRxiv - Developmental Biology 2022Quote: ... IGM generated sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, New England Biolabs). A polyA enrichment step (E7490 ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared for metatranscriptomic sequencing using the NEB-Next Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) with the addition of an rRNA depletion step (NEBNext rRNA Depletion Kit (Human/Mouse/Rat) ...
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bioRxiv - Genomics 2022Quote: ... The remaining steps of library preparation were done using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers from New England BioLabs were employed ...
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bioRxiv - Microbiology 2022Quote: ... 5 ng of total RNA was used for intact RNA library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760, New England BioLabs). To obtain sufficient RNA for the analysis of oral samples ...
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bioRxiv - Microbiology 2022Quote: RNA library was assembled using NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) and NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770, New England Biolabs) as per manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: ... Then we performed the bead-on T7 transcription reaction by HiScribe™ T7 High Yield RNA Synthesis Kit (NEB E2040S). The amplified RNAs were processed to the PCR-cDNA sequencing kit (nanopore) ...
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bioRxiv - Microbiology 2022Quote: ... Individual fragments were assembled by Gibson assembly at 50°C for 1 h in a total reaction volume of 20 µl (NEBuilder HiFi DNA Assembly Cloning Kit, New England Biolabs), thereby adding 6x His-tag coding sequences to the 3’ end of ply007 ...
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bioRxiv - Microbiology 2022Quote: ... RNA was then diluted to 20 ng/uL and 20-60 ng of RNA was used for qPCR using Luna universal one-step RT-qPCR kit (NEB) as per manufacture’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Sperm small RNA was converted to cDNA using the NEBNext Small RNA Library Prep Kit (New England Biolabs; Ipswich, MA) and sequenced as 75nt single-end reads on the Illumina NextSeq 550 platform (San Diego ...
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bioRxiv - Genetics 2022Quote: ... PCR products were run on an Agarose gel and desired bands were isolated by gel extraction (QIAquick Gel Extraction Kit, New England Biolabs) and were eluted in ddH2O ...
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bioRxiv - Biochemistry 2022Quote: ... Six histidine residues were added to the C-terminus of the sequence via the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). The resulting vector was transformed into OverExpress C41(DE3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were prepared from the cDNA using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Libraries were sequenced with the Illumina HiSeq 2500 or 4000 systems to produce 100bp paired-end reads ...
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bioRxiv - Biochemistry 2022Quote: In vitro protein translation was carried out (in absence of the release factors) using the PURExpress ΔRF123 kit (New England Biolabs). This guaranteed the stabilization of the ternary complexes mRNA-affitin-Ribosome ...
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bioRxiv - Neuroscience 2022Quote: ... DNA fragments were generated by PCR amplification and then fused with the backbones using NEBuilder HiFi DNA assembly kit (New England Biolabs). All plasmids were verified by sequencing (GeneWiz ...
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bioRxiv - Microbiology 2022Quote: ... The extracted RNA was then reverse transcribed to cDNA using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... digestion at 37°C for 30 minutes and then purified with the Monarch RNA Cleanup Kit (T2050, New England Biolabs) for long RNA (1020 nucleotides to 4187 nucleotides ...
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bioRxiv - Microbiology 2022Quote: ... The amplified fragment was cloned into BamHI site of pQF using an NEBuilder HiFi DNA assembly cloning kit (New England Biolabs) to generate pQFacvCDE ...
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bioRxiv - Microbiology 2022Quote: ... Subgenomic replicons of GLT1-20M and GLT1cc were constructed with the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs) according to the manufacturer’s instructions using oligonucleotides as specified in Table S1.
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bioRxiv - Immunology 2022Quote: ... Subsequently the libraries were prepared with the NEBNext® Ultra II DNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s protocol ...