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Citations for New England Biolabs :
6401 - 6450 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... an 887bp portion of mtDNA containing nd-1 was amplified by PCR and TA-cloned into pMiniT2.0 using the NEB PCR cloning kit (NEB E1202S). Purified plasmid was linearized with BamHI-HF (NEB 3136) ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was converted to a sequencing ready library using the NEBNext Ultra II Directional RNA library kit (New England Biolabs). The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for Illumina library quantitation (Kapa Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: ... 30 ng of total RNA was used for next-generation library preparation under the guidelines of the NEBNext Ultra DNA Library Prep Kit for Illumina (E7370; NEB). The libraries were loaded for 2 × 150 bp pair-end sequencing using Illumina Hiseq 2500 ...
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bioRxiv - Genetics 2022Quote: ... The resulting gDNA fragments were prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). Bead-based size selection was used to enrich for 200 bp fragments prior to a 21-cycle splinkerette PCR utilizing a custom Tn5-enriching forward primer (oAM055 ...
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bioRxiv - Cell Biology 2022Quote: ... Ravikant Ranjan) was digested using BglII and EcoRV and TgVPS15 cDNA was ligated in it using Gibson assembly kit (NEB) to obtain pCT-TgVPS15-iCWT ...
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bioRxiv - Cell Biology 2022Quote: ... pIREShyg2-HA-hAdipoR2-cMYC(H348A) construct was generated using PCR-based mutagenesis (Q5-site-directed mutagenesis kit, New England Biolabs) with the following primers ...
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bioRxiv - Biochemistry 2022Quote: ... Purified RNAs were used to make the RNA-seq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7760, NEB), followed by RNA-seq ...
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bioRxiv - Genomics 2022Quote: ... we prepared libraries for adaptive nanopore sequencing using the Ligation Sequencing Kit (Oxford Nanopore Technologies, ONT) and the NEBNext Companion Module for ONT Ligation Sequencing (New England Biolabs). 3 μg gDNA was used as input for these libraries ...
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bioRxiv - Genomics 2022Quote: ... After 25 cycles of amplification the product was purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030), and eluted with 20 μl of ddH2O ...
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bioRxiv - Molecular Biology 2022Quote: ... 40 μL of each digest product were then ran on a 3% agarose gel and the appropriate band was gel extracted using a kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 40 μL of digest product was then ran on a 3% agarose gel and the appropriate band was gel extracted using a kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: Paired-end libraries were prepared according to the manufacturer’s recommendations using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). The libraries were indexed with NEBNext Multiplex Oligos kit for Illumina (96 Index Primers ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA libraries were prepared for each individual titration point using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7370, New England Biolabs) and barcoded using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 & 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C for up to one month until used for purification of total RNA with the Monarch Total RNA Miniprep Kit (NEB). Five larvae were pooled for each RNA purification and homogenized in DNA/RNA protection buffer with a glass homogenizer prior to proteinase K digestion ...
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bioRxiv - Developmental Biology 2022Quote: ... and the reaction was purified with the Monarch RNA Cleanup Kit (500 micrograms) with elution in 50 µl nuclease-free water (NEB). The quality of the gRNA prep was confirmed by agarose gel electrophoresis and quantified using a NanoDrop One (Thermo Fisher).
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bioRxiv - Molecular Biology 2019Quote: ... quantified by qPCR using the NEBNext® Library Quant Kit for Illumina® (New England Biolabs® Inc., MA, USA), and fragment size (330 bp ...
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bioRxiv - Cell Biology 2019Quote: ... we introduced flanking FRT sites 104bp upstream of the transcription start site and 99bp downstream of the end of the 3’UTR using a Q5 Site-Directed Mutagenesis kit (New England Biolabs) and the following primers:
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bioRxiv - Molecular Biology 2019Quote: Sequencing libraries were prepared using NEBNext® Fast DNA Library Prep Set for Ion Torrent(tm) Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fragmentation of RNA followed by reverse transcription and second strand cDNA synthesis was performed using NEBNext Ultra RNA Library Prep Kit for Illumina (cat. No. E7530, NEB). The resulting double-stranded cDNA’s were further processed to DNA sequencing libraries using ThruPLEX DNA-seq 12S Kit (Rubicon Genomics ...
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bioRxiv - Physiology 2019Quote: ... RNA library was prepared using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing is performed on Illumina NextSeq 550 System ...
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bioRxiv - Biochemistry 2019Quote: ... TruSeq-barcoded RNAseq libraries were generated from half of the rRNA-subtracted samples with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit 2.0 (dsDNA HS kit ...
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bioRxiv - Biophysics 2019Quote: ... Site directed mutagenesis of the HEV510-696 was performed using primers detailed in Table 1 and the Q5 Site-directed Mutagenesis Kit (NEB).
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bioRxiv - Molecular Biology 2019Quote: ... Half of the eluted RNA was used for strand-specific RIP-Seq library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs).
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bioRxiv - Genetics 2019Quote: Illumina libraries with TruSeq barcode adapters were prepared using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) on the 1/4 scale of the manufacturer-suggested protocol ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
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bioRxiv - Microbiology 2019Quote: ... end prepped with dA tailing Kit (NEBNext® Ultra™ End Repair/dA-Tailing Module, New England Biolabs, MA, USA), bead purified (1:1 ...
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bioRxiv - Molecular Biology 2019Quote: Illumina RNA sequencing libraries from purified mRNA were prepared and sequenced by the Centre for Genomic Research at University of Liverpool using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Paired-end sequencing with a read length of 150 bp was carried out on an Illumina HiSeq 4000 ...
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bioRxiv - Genetics 2019Quote: ... The donor sequence was generated by subcloning 596 bp upstream of the hrpk-1 stop codon and 600 bp downstream of hrpk-1 stop codon into the pDD282 vector [45] using Hi Fi assembly kit (NEB). The hrpk-1 stop codon was eliminated from the donor sequence to allow in frame GFP tag addition ...
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bioRxiv - Plant Biology 2019Quote: Approximately 1 ug of RNA was used as input to the NEB Ultra II RNA sequencing library kit for illumina (New England Biolabs, NEB catalogue number ...
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bioRxiv - Plant Biology 2019Quote: Approximately 1 ug of RNA was used as input to the NEB Ultra II RNA sequencing library kit for illumina (New England Biolabs, NEB catalogue number ...
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bioRxiv - Molecular Biology 2019Quote: ... Size selection was performed by running samples on a 1.8% LMT agarose gel at 80 V for 40 minutes at room temperature and gel extracting the mononucleosome band with a Monarch Gel Extraction Kit (T1020S, New England Biolabs). Note that of the samples digested with 10 ...
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bioRxiv - Genetics 2019Quote: ... The amplicons were gel-purified on a 4% agarose gel and assembled with the cut Lenti-gRNA-Puro plasmid described above using an NEBuilder HiFi DNA Assembly kit (NEB). After incubation at 50 °C for 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... The remaining 50 μL was used as input for one round of end repair and adapter ligation with NEBNext Ultra II DNA Library Preparation kit (NEB), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and wobble mutations to make constructs resistant to shOTUD5#5 were introduced in this vector using the Q5 site-directed mutagenesis kit (E0554, NEB) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Most point mutants and wild-type variants were generated from this vector following the Q5 Site-Directed Mutagenesis Kit protocol (E0554, New England Biolabs) and primers 1-8 (Sup ...
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bioRxiv - Genomics 2019Quote: ... polyA+ RNA and polyA-RNA were then made into a strand specific library using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturers protocol (utilizing a 15-minute fragmentation step ...
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bioRxiv - Molecular Biology 2019Quote: ChIP-seq libraries for both ChIP and input samples were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB), following the manufacturer’s guidelines and using NEBNext Multiplex Oligos ...
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bioRxiv - Physiology 2019Quote: ... The suicide delivery vector pEMG was isolated using the NEB Monarch Plasmid Miniprep Kit (New England Biolabs, Ipswich, MA, USA). The isolated plasmid was digested with restriction enzymes purchased from New England Biolabs (Ipswich ...
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bioRxiv - Synthetic Biology 2019Quote: ... Reagents and competent DH5a cells used for cloning were from the NEBuilder HiFi DNA Cloning Kit (Product E5520S New England Biolabs). Q5® High-Fidelity 2X Master Mix was used for long PCRs (Product M0492SNew England Biolabs 240 County Road Ipswich ...
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bioRxiv - Cell Biology 2019Quote: ... the pGL4.10-P plasmid was mutated by PCR using mutant primers and Q5 site-directed mutagenesis kit (New England Biolabs, E0554) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The assembly reaction of 1:5 vector:insert ratio was carried out for 1 hour at 50C using NEBuilder HIFI DNA assembly kit (New England Biolabs, NEB). Assembly products were electroporated into NEB Turbo Competent E.Coli (NEB ...
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bioRxiv - Genomics 2019Quote: ... The assembly reaction of 1:5 vector:insert ratio was carried out for 1 hour at 50C using NEBuilder HIFI DNA assembly kit (New England Biolabs, NEB). Assembly products were electroporated into NEB Turbo Competent E.Coli (NEB ...
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bioRxiv - Biophysics 2019Quote: ... v/v) and then prepared for Illumina sequencing with the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs). The adaptor-ligated DNA was amplified for 10 cycles following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We treated DNA extractions with 2 μl of RNAase A (ThermoScientific) and purified them with Monarch PCR & DNA Cleanup Kit (New England Biolabs). We assessed DNA quantity and quality with Qubit 3.0 (Thermofisher) ...
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bioRxiv - Developmental Biology 2019Quote: ... Library preparation was carried out using 0.5 μg of RNA with NEBNext Ultra RNA library Prep Kit for Illumina (New England BioLabs, E7530L). For RNA-seq from E7.5 embryos ...
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bioRxiv - Developmental Biology 2019Quote: ... and was used to prepare sequencing libraries with NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolabs). Briefly ...
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bioRxiv - Plant Biology 2020Quote: RNA-seq libraries were generated from 1µg of input RNA using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7490) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2019Quote: RNA was reverse-transcribed with random priming using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) with 5ul sample input ...
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bioRxiv - Synthetic Biology 2019Quote: ... The amplicon corresponding to a genomic fragment of 1240 bp was run on a gel and the DNA was excised and purified with Monarch DNA Gel Extraction Kit (NEB). DNA concentration and absorbance ratio were measured with a NanoDrop Spectrophotometer (ThermoScientific) ...
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Replication Timing and Transcription Identifies a Novel Fragility Signature Under Replication StressbioRxiv - Genetics 2019Quote: ... Sequencing libraries of BrdU-substituted DNA from early and late fractions were prepared by NEBNext Ultra DNA Library Prep Kit for Illumina (E7370; New England Biolabs). Sequencing was performed on an Illumina-HiSeq 2500 sequencing system by 50-bp single-end reads ...