Labshake search
Citations for New England Biolabs :
6051 - 6100 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: Each RNA-sequencing library was prepared from 200 ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra RNA Library Prep Kit (New England Biolabs), following the manufacturer’s instructions and selecting for ~350 bp size fragments ...
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bioRxiv - Developmental Biology 2019Quote: ... the Cas9 site of the repair template was modified by introducing synonymous mutations either directly into the primers used for fragment amplification or separately by Q5 site directed mutagenesis kit (New England Biolabs). All DNA plasmids used for genome editing were transformed into DH5α competent cells and subsequently purified by miniprep (PureLink Quick Plasmid Miniprep Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... and family 2 baby 10 month) we performed enrichment with the NEB Next Microbiome Enrichment Kit (New England Biolabs, www.neb.com) and sequenced both the pre-enrichment and post-enrichment samples ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid #1129 used to obtain transgenic fish line expressing LifeAct-frFAST fusion under the control of the cmlc2 enhancer28 was constructed using the NEBuilder assembly kit (New England BioLabs) in the pT22i vector for transgenesis previously used29.
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bioRxiv - Microbiology 2020Quote: Reverse transcription and amplification for figure 2 was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S). Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat ...
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bioRxiv - Molecular Biology 2019Quote: ... and the pSpCas9(BB)-2A-mCherry (generated in house, by replacing the GFP with a mCherry reporter using the NEBuilding HiFi DNA Assembly Cloning Kit [New England Biolabs]). All sgRNAs sequences can be found in table S1.
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bioRxiv - Plant Biology 2019Quote: ... We constructed individually indexed genomic libraries using the NEBNext Ultra DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA), which were then target enriched8 and sequenced using a 1/3 lane of 150 bp paired ends on a HiSeq 3000 at the Oregon State University Center for Genome Research and Biocomputing Central Services Lab.
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bioRxiv - Evolutionary Biology 2019Quote: ... Libraries were prepared using the NEBnext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and polyA enriched ...
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bioRxiv - Genetics 2019Quote: ... in accordance with the manufacturer’s recommendations and were validated using the Agilent High Sensitivity DNA assay on the Agilent Bioanalyzer 2100 system and quantified by NEBNext Library Quant Kit for Illumina (New England Biolabs). After clustering of the index-coded samples ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were cleaned up using the Monarch® PCR and DNA clean-up kit (New England Biolabs, USA). The PCR samples were mixed with 125 μl of the DNA Clean-Up Binding Buffer ...
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bioRxiv - Microbiology 2019Quote: ... samples were diluted to 5ng/uL and used for qRT-PCR with Luna One-Step Universal qPCR kit (NEB E3005). Gene specific primers for target transcripts were used at a final concentration of 400uM with 15ng of RNA ...
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bioRxiv - Genomics 2019Quote: ... Sequencing adapters and oligonucleotides used for PCR barcoding were from the NEBNext Multiplex Oligos for Illumina Kit (New England Biolabs, NEB). Prior to PCR amplification of the library ...
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bioRxiv - Developmental Biology 2020Quote: ... a total amount of 2µg of RNA per sample was used to prepare poly(A+)-enriched cDNA libraries using the NEBNext Ultra RNA library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Strand-specific RNA-Seq libraries were constructed using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, catalogue number: E7420). The resulting library concentrations were quantified using the Nanodrop ...
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bioRxiv - Genetics 2019Quote: ... with a KAPA Hyper Prep Kit (KAPA, kk8502) and then processed by 3 μl of USER™ Enzyme (NEB, M5505L) at 37°C for 15 min to open up the loop ...
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bioRxiv - Genomics 2019Quote: ... followed by purification with Ampure beads and mRNA synthesis with the HiScribe™ T7 ARCA mRNA Kit (Cat. E2065S, NEB). Then the in vitro transcribed mRNAs were treated with DNase I (NEB ...
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bioRxiv - Genomics 2020Quote: ... was transcribed from a sgRNA-coding PCR product with a 5′ T7 promoter sequence using HiScibe T7 Quick High yield RNA Synthesis kit (NEB). The transcription was performed at 37 °C overnight and then purified by phenol ...
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bioRxiv - Neuroscience 2019Quote: ... RNA-seq libraries were prepared from the mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Cluster generation was performed with the resulting libraries using the Illumina TruSeq PE Cluster Kit v4 reagents and sequenced on the Illumina HiSeq 2500 using TruSeq SBS Kit v4 reagents (Illumina) ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNA-seq libraries were prepared with the NEBNext Ultra RNA library preparation kit with poly(A) mRNA magnetic isolation module (NEB) essentially according to manufacturer protocols ...
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments were ligated to the Barcode Adapter from the barcode Kit 1D (EXP-PBC001, Nanopore) using Blunt/TA Ligase Master Mix (M0367L, NEB). Following purification with AMPure XP beads ...
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bioRxiv - Genetics 2020Quote: ... ChIP enrichment was determined by RT-qPCR (Supplementary Table 5) prior to addition of sequencing adaptors using NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs). ATAC-seq was performed as previously described76 ...
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bioRxiv - Genetics 2020Quote: ... all wells from a single plate were pooled and prepared for sequencing with the NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs). Plates were multiplexed and sequenced on the MiSeq platform (Illumina ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNF114 C8A mutant was generated with Q5 site-directed mutagenesis kit according to manufacturer’s instructions (New England Biolabs, E0554S). Expression and purification conditions were optimized as reported previously79 ...
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bioRxiv - Genomics 2019Quote: ... or 100 ng of gDNA was assembled into paired-end libraries using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs). Approximately 60 libraries were pooled per lane prior to sequencing on a HiSeq 4000 machine to a target depth of 0.5×.
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bioRxiv - Systems Biology 2019Quote: ... RNA-Seq libraries were prepared from 1 μg of purified RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs). Sequencing was performed on an Illumina NextSeq 500 (75 cycles) ...
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bioRxiv - Molecular Biology 2019Quote: ... and set up in an overnight in vitro transcription reaction (HiScribe T7 Quick High Yield RNA Synthesis kit, New England BioLabs). The resulting RNA was purified using Silane beads (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Insert and dual indexed libraries were prepared using a modified NEBNext Ultra DNA II dual indexing kit (New England Biolabs) without size selection ...
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bioRxiv - Microbiology 2019Quote: ... Second-strand cDNA was synthesized in a reaction mixture containing Second-Strand Synthesis Kit (New England Biolabs; Ipswich, MA, USA). cDNA was purified using the Qiagen purification kit ...
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bioRxiv - Microbiology 2019Quote: Site-directed mutagenesis of low copy number vector p3187 harbouring siiA::HA under control of PsiiA was performed by using the Q5 SDM kit according to the manufacturer instructions (NEB) (Table S9) ...
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bioRxiv - Cell Biology 2019Quote: ... The CLN6ΔL2 construct was obtained by removing the codons for amino acids 155-222 using the Q5 Site-Directed Mutagenesis kit (New England Biolabs).
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bioRxiv - Genomics 2019Quote: ... Immunoprecipitated and input material was phenol-chloroform purified and ChIP-seq libraries were prepared using the NEBNext Ultra II DNA library kit (NEB) according to the manufacturer’s instructions with 35ng of DNA of each sample ...
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bioRxiv - Bioengineering 2019Quote: ... The pgRNAMPN142-400 plasmid is constructed by removing the gRNAMPN372 from the pgRNA-triple-target plasmid using the Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Bioengineering 2019Quote: ... and 21 µg of gRNA transcripts produced by in vitro transcription of oligonucleotides (HiScribeTM T7 High Yield RNA Synthesis kit from NEB). Plugs are then treated overnight at 50°C with 10 µL of proteinase K ...
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bioRxiv - Microbiology 2019Quote: ... DNA-depleted RNA was processed for Illumina sequencing using the NEB Next Ultra Directional RNA Library Prep kit for Illumina as described by the manufacturer (NEB). Sequencing was performed using Illumina HiSeq ...
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bioRxiv - Molecular Biology 2019Quote: ... Then these two fragments were assembled into the modified pIRES vector at AsisI and PmeI sites using Gibson Assembly kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... RNA-seq libraries were prepared using NEB’s NEBNext Ultra II Directional RNA Library Prep Kit for Illumina Sequencing (NEB EE7760S) and following the instructions for the poly(A ...
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bioRxiv - Biochemistry 2019Quote: ... were generated as described previously.23 In vitro transcription/translation of (fMVF-Flag was carried out using the combination of PureExpress (ΔtRNA, Δaa (E6840S)) and PureExpress (Δribosome (E3313S)) kits (NEB) with the following modifications ...
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bioRxiv - Microbiology 2019Quote: ... Library quality was assessed using an Agilent Tapestation 4200 instrument and quantity determined by qPCR using an NEBnext library quantification kit (NEB).
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bioRxiv - Biochemistry 2021Quote: cRNA were synthesized in vitro from linearized DNA of EGFP-fused mTTYH constructs using HiScribe T7 ARCA mRNA Kit (New England Biolabs). 1–2 ng of cRNA were injected in Xenopus laevis oocytes and imaged after 18 hours of incubation at 18 °C ...
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bioRxiv - Microbiology 2021Quote: ... primers oJV5-6 were used to remove ackA from pJV204 using the Q5 site-directed mutagenesis kit (NEB CN# E0554S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting cell pellets were washed in 1x PBS and resuspended in 1x DNA/RNA protection reagent (Monarch Total RNA Miniprep Kit, NEB). Subsequently ...
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bioRxiv - Genetics 2020Quote: ... CUT&RUN dsDNA samples were quantified with a Qubit III and 5-10ng used as input for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (#E7645S, NEB). Libraries were prepared using the following PCR program ...
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bioRxiv - Genetics 2021Quote: ... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
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bioRxiv - Genomics 2021Quote: Wild-type HAP1 samples were used for standard ChIP-seq library preparation with NEBNext Ultra II DNA Library Prep kit (New England Biolabs), followed by sequencing on NovaSeq 6000 ...
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bioRxiv - Genomics 2021Quote: ... Shotgun sequencing was performed using a PCR-free DNA library preparation (NEBNext Ultra II DNA Library Prep Kit, New England Biolabs). Libraries were paired end 150 bp sequenced with Illumina HiSeqX ...
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bioRxiv - Genetics 2019Quote: ... and non-directional high-throughput sequencing libraries were prepared using NEBNext Ultra DNA Library Prep Kit (New England Biolabs, E7370). Libraries were processed on the Illumina HiSeq platform for single-end 50-nt sequencing ...
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bioRxiv - Genetics 2019Quote: ... and used to construct DNA sequencing libraries using NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs E7760). DNA libraries were processed on a Illumina NextSeq machine for paired-end 42-nt sequencing ...
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bioRxiv - Genomics 2021Quote: ... Libraries were purified with SPRI beads (0.9X) and quantified on a bioanalyzer and with NEB Illumina quant kit (NEB#E7630). HiC libraries were sequenced on a NextSeq500 either 150bp or 75bp paired-end reads.
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bioRxiv - Cancer Biology 2020Quote: The Flag-HA-USP18WT construct was purchased from Addgene (#22572) and the Flag-HA-USP18C64R/C65R construct was generated using the Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) and the primers OL87 ...
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bioRxiv - Genomics 2021Quote: The RNA-seq libraries were prepared as follows: the rRNA was depleted from 0.5 µg of A549 total RNA using the NEBNext rRNA Depletion Kit (NEB E6310). The resulting rRNA-depleted RNA was used for library construction with the NEB ultra II directional RNA-seq kit (NEB E7760) ...