Labshake search
Citations for New England Biolabs :
6151 - 6200 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... The resulting fragments were gel-purified with the QIAquick Gel Extraction Kit and ligated using T4 DNA Ligase (New England Biolabs) into dephosphorylated pBlueScript KS+ ...
-
bioRxiv - Plant Biology 2021Quote: ... IPT3 promoter DNA sequence was extracted and purified from the gel using Monarch DNA Gel Extraction Kit (New England Biolabs) and Sanger sequenced at Genewiz (South Plainfield ...
-
bioRxiv - Plant Biology 2021Quote: ... Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses were performed using Luna Universal Probe One-Step qRT-PCR Kit (New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... About 10ng of the purified CUT&RUN DNA was used for preparation of multiplexed libraries with the NEB Ultra II DNA Library Prep Kit per manufacturer’s instruction (NEB #E7103). Sequencing was conducted using an Illumina NextSeq 500 Sequencing System (available from the core facility of UNC Pharmacology Department).
-
bioRxiv - Biochemistry 2021Quote: ... 5’-TAATCAGACAAGGAACTGATTA-3’ (Forward) and 5’-CGAAGGTGTGACTTCCATG-3’ (Reverse) were used with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in an Applied Biosystems QuantStudio 6 thermocycler ...
-
bioRxiv - Biochemistry 2021Quote: ... The LAMP reaction in the 2-step LAMP-CRISPR detection was performed using the WarmStart® LAMP Kit (NEB #E1700), using primer concentration described in literature (“Rapid Detection of SARS-CoV-2 Using Reverse transcription RT-LAMP method,” 2020) ...
-
Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: Frameshifting reporter as well as positive and negative control mRNAs were in vitro transcribed and polyadenylated using HiScribe T7 mRNA kit (New England Biolabs) according to the manufacturers’ instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... Genomic RNA fragments (Table S1) were synthesized following the protocol of HiScribe T7 high yield RNA synthesis kit (NEB, E2040S) with 1 μg purified PCR products ...
-
bioRxiv - Bioengineering 2021Quote: ... RNA was purified using the Macherey Nagel RNA extraction kit following manufacturer’s instruction and viral RNA uptake was quantified using the Luna universal One-Step RT-qPCR kit (NEB; E3005).
-
bioRxiv - Cell Biology 2021Quote: ... Single guide RNAs (sgRNAs) were synthesized using the EnGen sgRNA Synthesis Kit (for the Streptococcus pyogenes-originated Cas9, New England Biolabs). The SpCas9-sgRNA ribonucleoprotein (RNP ...
-
bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were constructed with the NEBNext® Ultra™ II DNA Library Prep kit (New England Biolabs, MA, USA), and sequenced by Illumina-MiSeq at the Pittsburgh Bacteriophage Institute to an approximate shotgun coverage of 3538x ...
-
bioRxiv - Plant Biology 2020Quote: ... Size-selected DNA fragments were used to prepare eight MNase sequencing libraries with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB), following manufacturer’s instructions (2 biological replicates x 2 MNase concentrations x 2 tissues) ...
-
bioRxiv - Plant Biology 2020Quote: Sequencing libraries were generated from poly(A)-enriched RNA using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Equal amounts of RNA (~50 ng) were used for preparation of cDNA libraries with the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB) with some minor changes to the manufacturers’ instructions ...
-
bioRxiv - Systems Biology 2021Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
-
bioRxiv - Microbiology 2020Quote: ... these long gene sequences containing enzyme sites firstly was amplified by common PCR by Q5 high-fidelity PCR kit (Cat: #M0491, NEB), using primer F and R pairs of S or N ...
-
bioRxiv - Systems Biology 2020Quote: ... Library preparation was performed using the NEBNext®Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs), and library quantification was performed using the KAPA library quantification kit (Kapa Biosystems) ...
-
bioRxiv - Systems Biology 2020Quote: The extracted plasmids were amplified using “lib-seq” primers in the Supplementary Table 6 for 10 cycles using Phusion PCR kit (NEB). Each 50 μL PCR reaction consisted of 6 μL of plasmid library ...
-
bioRxiv - Systems Biology 2020Quote: ... We neutralized the tagmentation activity of Enzyme 1 by immediately cleaning the reaction with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Systems Biology 2020Quote: RNA-seq libraries were prepared using 200 ng of total RNA and the NEBNext rRNA Depletion Kit (NEB, Cat: E6310X), per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: Viral RNA genome for both WT ZIKV and the X1 mutant was in vitro transcribed from ligated plasmid DNA using a HiScribe T7 ARCA mRNA kit (NEB). Approximately 40 μg of the resulting mRNAs were transfected into Vero cells using MessengerMAX lipofectamine transfection reagent (Invitrogen) ...
-
bioRxiv - Plant Biology 2021Quote: ... and the purified mRNA was used to build sequencing libraries using the Ultra II Directional RNA library Prep Kit for Illumina (NEB). Single end sequencing of 76 bp were performed on NextSeq instrument (Illumina) ...
-
bioRxiv - Microbiology 2020Quote: RNA samples were confirmed to be DNA-free by conducting test PCRs on the 16S rRNA gene and converted to cDNA using the LunaScript RT SuperMix Kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... pLenti-puro construct expressing N-terminal HA-tagged human SAMHD1 was described previously8 and was used as template to generate mutants using the Q5® Site-Directed Mutagenesis Kit according to the manufacturer’s instructions (NEB). To obtain the SAMHD1ΔC-SUMO fusion proteins ...
-
bioRxiv - Microbiology 2020Quote: ... 1 μl of the diluted cDNA was used in 10 μl reaction mixture of Q5 Hot START DNA Polymerase kit (NEB) (2 μl of 5X buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
-
bioRxiv - Microbiology 2020Quote: ... column purified (QIAquick PCR Purification kit) and ligated with restriction enzyme cut-pHH21 using T4-ligase (New England Biolabs, NEB). Ligation products were transformed into DH5a (NEB ...
-
bioRxiv - Microbiology 2020Quote: ... column purified (QIAquick PCR Purification kit) and ligated with restriction enzyme cut-pHH21 using T4-ligase (New England Biolabs, NEB). Ligation products were transformed into DH5a (NEB ...
-
bioRxiv - Microbiology 2021Quote: ... the codon optimized RBD was PCR amplified and cloned into the bicistronic pDZ rescue plasmid system for A/Puerto Rico/8/1934 using the NEBuider HiFi DNA assembly kit (NEB). Specifically ...
-
bioRxiv - Genomics 2020Quote: ... The first step involved incubation of total DNA with proteinA-MBD2-Fc mixture (NEBNext Microbiome DNA Enrichment Kit, New England BioLabs, Microbial enrichment kit ...
-
bioRxiv - Genomics 2020Quote: ... Samples were prepared for Illumina sequencing with NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (NEB #E7775) accompanied by NEBNext Poly(A ...
-
bioRxiv - Genomics 2021Quote: ... C and 5mC deamination reaction was performed using the APOBEC3A enzyme (NEBNext® Enzymatic Methyl-seq Kit, New England Biolabs) with the following ramping conditions ...
-
bioRxiv - Genetics 2021Quote: ... Repair templates were generated by assembling homology arms (450-bp gBlocks synthesized by IDT) and the selection cassette using the NEBuilder Hifi DNA Assembly Kit (New England Biolabs).
-
bioRxiv - Microbiology 2021Quote: ... cDNA was amplified and indexed using the PCR Barcoding Kit (Oxford Nanopore Technologies) and LongAmp Taq 2X master mix (New England Biolabs). The resulting cDNA libraries were sequenced on a MinION (MIN101B ...
-
bioRxiv - Microbiology 2021Quote: ... and sequencing libraries were generated using the rRNA-depleted RNA samples using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs). Briefly ...
-
bioRxiv - Microbiology 2021Quote: ... pcineo NS1 single site mutants in the full-length construct were generated by using the Q5 site-directed mutagenesis kit (New England BioLabs), following the manufacturer recommendations ...
-
Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltiibioRxiv - Genetics 2021Quote: ... DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library kit for Illumina (New England Biolabs). All volumes specified in the manufacturer’s protocol were divided by four ...
-
bioRxiv - Microbiology 2021Quote: ... was digested with NotI and NheI and the mgrB PCR product was cloned using the NEBuilder HiFi DNA assembly kit (NEB), followed by transformation into the E ...
-
bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RNA levels were quantified with 250 ng of RNA inputs using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), using real-time RT-PCR primer/probe sets 2019-nCoV_N1 (CDCN1 ...
-
bioRxiv - Immunology 2021Quote: ... pSFG-SARS-CoV-2 S D614G was cloned from pSFG-SARS-CoV-2 S plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). 293T cells were transfected with 3.75 μg pSFG-SARS-CoV-2 S or pSFG-SARS-CoV-2 S D614G ...
-
bioRxiv - Immunology 2021Quote: ... Point mutations and deletions of cGAS were constructed using a Q5® Site-Directed Mutagenesis Kit (New England Biolabs, # E0554S).
-
bioRxiv - Immunology 2021Quote: ... The resultant DNA was cloned into a pUC19 plasmid and in vitro transcribed using a HiScribe T7 High Yield RNA synthesis kit (New England Biolabs). After DNAseI digestion and clean-up with a TURBO DNA-free kit (Thermo-Fisher) ...
-
bioRxiv - Immunology 2021Quote: ... the pCMV3-SΔ19 insert was initially digested with KpnI and blunt polished using Phusion Taq polymerase followed by a DNA cleanup using the Monarch PCR cleanup kit (NEB) and a second digest was done using NotI ...
-
bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were created using the NEBNext Ultra II DNA Library Prep kit for Illumina sequencing (New England Biolabs E7645L). All cDNA and library clean-up was done using SPRIselect beads (Beckman Coulter B23317).
-
bioRxiv - Genomics 2021Quote: ... A reporter containing the alternate allele was generated through SDM using the Q5 Site-Directed Mutagenesis kit (New England Biolabs). The primer sequences used were as follows:
-
bioRxiv - Genomics 2021Quote: ... Libraries were prepared using NEBNext Poly(A) mRNA magnetic isolation module and NEB Ultra Directional RNA Library kit for Illumina (New England Biolabs).
-
bioRxiv - Microbiology 2020Quote: ... A bacteria-codon optimized gBlock for the truncated protein was produced by IDT DNA Technologies and cloned into a pET28a bacterial expression with a C-terminal 6xHis tag using the NEBuilder Assembly Kit (New England Biolabs). Recombinant protein was expressed in BL21(DE3 ...
-
bioRxiv - Microbiology 2020Quote: ... The LPMO and CBH1 amplicons for detection were radio-labelled with [α-32P] dCTP using the NEBlot Kit (NEB, USA) according to the manufacturer’s instructions and used as a probe to determine the copy number of T-DNA integrations in all the transformants.
-
bioRxiv - Microbiology 2020Quote: DNA was extracted from high-titer phage lysates and sequencing libraries were prepared using the Ultra II FS Kit (New England Biolabs) or for ARB14 and ARB25 ...
-
bioRxiv - Molecular Biology 2020Quote: Many of the reagents used in 5XP-seq are included in the NEBNext Multiplex Small RNA Library prep kit for Illumina (New England Biolabs), and are referred to as ‘NEB-kit’ below ...