Labshake search
Citations for New England Biolabs :
6001 - 6050 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... The amplicons were purified and subjected to error-prone PCR under the following conditions: 5 U Taq DNA polymerase (New England Biolabs, MA, USA), 200 μM of each deoxynucleoside triphosphate ...
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bioRxiv - Neuroscience 2024Quote: ... Sequence encoding the modified capsid insertion was amplified using serotype-specific primers with PCR cycles less than 30 by using Q5 2x Master Mix (NEB, Ipswich, MA). Two microliters of 1st round of PCR products were input to total 50 µl reaction of second round PCR to introduce Illumina i5 and i7 sequencing indices ...
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bioRxiv - Microbiology 2024Quote: ... The variable region V3+V4 of the 16S rRNA gene was amplified using a broad-range primer pair (338F: 5’-ACTCCTACGGGAGGCAGCA-3′, 806R:5′-GGACTACHVGGGTWTCTAAT-3′) using the Phusionâ High-Fidelity PCR Master Mix (New England Biolabs, Beverley, MA). The PCR amplification program was as follows ...
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bioRxiv - Microbiology 2024Quote: ... single colonies were picked and used as a template for amplification by PCR using Q5 DNA polymerase (New England Biolabs, Whitby, Canada) with primers CR1_F and R as well as CR3_F and R (Supplementary Table 2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... were prepared using the NEBNext Ultra II DNA Prep Kit following the published protocol for this kit (New England Biolabs, Ipswitch, MA, USA), and quantified using an Agilent Bioanalyzer 2100 DNA 1000 kit (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we isolated DNA from tissue using the Qiagen DNeasy Blood and Tissue kit (Valencia, CA, USA) and created genomic libraries using the NEBNext Ultra II kit (New England BioLabs; Ipswich, MA, USA), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... to evaluate the performance of our library preparation protocol – TM3’seq – and compare it to the performance of a commercial kit commonly used to generate RNA-seq libraries – NEBNext® Ultra™ Directional RNA kit (NEB, #E7420S). Three replicates of 200ng total RNA per tissue (blood and adipocyte ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cloning was carried out using the Gibson Assembly® Master Mix kit and NEBuilder® HiFi DNA Assembly kit (New England Biolabs, US). Genomic DNA was prepared by the Blood & Cell Culture DNA Kit (QIAGEN ...
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bioRxiv - Genomics 2020Quote: ... The first step involved incubation of total DNA with proteinA-MBD2-Fc mixture (NEBNext Microbiome DNA Enrichment Kit, New England BioLabs, Microbial enrichment kit, New England Biolabs, Cat No. E2612L), which binds and depletes methylated nuclear DNA fragments ...
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bioRxiv - Microbiology 2021Quote: ... gDNA harvested with the Masterpure Complete DNA/RNA Purification Kit was bisulfite treated using the EpiMark Bisulfite Conversion kit (NEB; Ipswich, MA, USA); both per manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... rRNA was depleted from total RNA using Ribo-Zero™ rRNA removal Kit and library was made using Illumina NEBNext® Ultra™ Directional RNA Library Prep Kit (E7420L, NEB). The libraries were loaded on an Illumina HiSeq X ten instrument (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... gDNA was extracted from 1 ml of overnight culture with the kit Monarch HMW DNA Extraction kit (T#3060 New England Biolabs; Ipswich, MA, USA) following the manufacturer instructions for High Molecular Weight DNA extraction from gram-negative bacteria ...
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bioRxiv - Genetics 2024Quote: ... The fragmented samples were purified with Expin™ PCR SV kit (GeneAll) and prepared as an NGS library with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB). The prepared samples were sequenced with MiniSeq High Output Reagent Kit (300-cycles ...
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bioRxiv - Genomics 2023Quote: ... Two libraries were prepared for Nanopore MinION sequencing using the Ligation Sequencing kit (SQK-LSK108, ONT, Oxford, UK) and NEBnext DNA Repair kit reagents (NEB, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: Libraries for each sample were prepared using the NEBNext Ultra II DNA Library Prep Kit and NEBNext Muliplex Oligos for Illumina Kits (New England Biolabs, Ipswich, Massachusetts, USA) following the NEBNext Ultra II Version 5 protocol with size selection on DNA fragments at 300-400bp range ...
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bioRxiv - Evolutionary Biology 2020Quote: Sequencing libraries were prepared using either the “NEBNext Ultra II DNA Library Prep Kit for Illumina®” (E7645) or the “NEBNext Ultra II FS DNA Library Prep Kit for Illumina®” (E7805) (New England BioLabs, Ipswich, USA). Sequencing was conducted on an Illumina MiSeq System (Illumina Inc. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The described method follows the NEBNext® Ultra™ II DNA Library Prep Kit (v4.0) with Sample Purification Beads Kit (New England Biolabs, Ipswich, Massachusetts, USA). But other kits can be used too ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or the NEBNext Ultra DNA Library Prep Kit for Illumina (formerly NEBNext DNA Library Prep Kit for Illumina; New England Biolabs Inc., Ipswich, Massachusetts, U.S.) and the NEBNext Multiplex Oligos for Illumina were used for library preparations ...
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bioRxiv - Developmental Biology 2019Quote: ... An amount of 500 ng total RNA was used for RNA-seq library preparation with NEB NEBNext rRNA Depletion Kit and ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs Japan Inc., Tokyo, Japan); 2 × 36 base paired-end sequencing was performed with NextSeq500 (Illumina K.K. ...
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bioRxiv - Neuroscience 2024Quote: ... Samples with a RIN greater than 7 were processed for library preparation with NEBnext rRNA depletion kit and ULTRAII FS RNA-seq Library Preparation Kit for Illumina (New England Biolabs, Ipswich, MA, United States). This procedure involved steps for mRNA enrichment ...
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bioRxiv - Immunology 2021Quote: ... and ROI 2 were amplified from C57BL/6 genomic DNA by PCR using the Q5® High-Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA). PCR products were ligated into pSCB-Amp/Kan using the StrataClone Blunt PCR Cloning Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: ... the first PCR was performed in triplicate with tailed forward and reverse primers using NEBNext Q5® Hot Start HiFi PCR Master Mix (New England BioLabs, Hitchin, UK) and 17 and 25 cycles for the bacterial and functional marker ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The double mutants TOPO-blaOXA-48-F72L/G131S and TOPO-blaOXA-48-N146S/L158P were created by inverse PCR using Phusion polymerase (New England Biolabs, Ipswich, MA, USA) and TOPO-blaOXA-48-F72L or TOPO-blaOXA-48-L158P as a template ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR products for 53bp1- and Usp28-mutant mice were digested with KpnI and ApoI restriction enzymes (New England BioLabs; Ipswich, MA, USA), respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... the EcoRI inverse PCR yielded a desired length of 2 kb of promoter sequence but for duck and quail less than 2 kb of the promoter was initially sequenced so inverse PCR was repeated using XbaI (R0145S, NEB, Ipswich, MA, USA).
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bioRxiv - Genomics 2020Quote: ... The A-tailed cDNA was amplified using two rounds of PCR amplification using Phusion High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA). The first round of amplification was done over 15 cycles ...
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bioRxiv - Neuroscience 2019Quote: ... Semi-quantitative analysis was conducted using standard PCR amplification (Q5 Hot-Start High-Fidelity DNA polymerase; NEB, Ipswich, MA, USA, and agarose (2%) gel electrophoresis ...
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bioRxiv - Cancer Biology 2021Quote: ... Purification of PCR products was performed with an in-house ExoSAP solution [50 μl of Exonuclease I (20,000 units/ml) (NEB® M0568, Ipswich, MA); 40 μl of Antarctic Phosphatase (5,000 units/ml) ...
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bioRxiv - Neuroscience 2019Quote: ... The cassette was amplified by PCR then ligated to the donor template using Gibson Assembly Master Mix (NEB, primers in Supplementary Table 3). The reaction was incubated at 50 °C for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and PCR-amplified XTEN80-mEGFP-3xHA immunoprecipitation tag with a linearized backbone generated from ClaI and BspDI (New England BioLabs cat. no. R0557) digestion of pKL017 (pHIV EF1a:Clover:WPRE) ...
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bioRxiv - Plant Biology 2021Quote: ... the variable regions V5-V7 of the bacterial 16S rRNA gene were amplified by PCR (NEBnext High Fidelity 2x Master Mix, New England Biolabs, Ipswich, MA, USA) using 10 ng of DNA per reaction and the primers 799F and 1193R from (Bulgarelli et al. ...
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bioRxiv - Neuroscience 2020Quote: ... with TdTomato was amplified by PCR with primers (5’- ggcgcgCCCCCCTCTCCCTCCCCCCC -3’ and 5’- ggcgcgccTTACTTGTACAGCTCGTCCATGCCGTACAG -3’) using Hot start Q5® polymerase (NEB, Frankfurt, Germany) from LeGO-iT (a gift from Boris Fehse ...
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bioRxiv - Plant Biology 2021Quote: ... The purified PCR products and the pLSU-1.1 plasmid were digested with KpnI-HF® and BamHI-HF® (New England Biolabs® Inc.). With the same restriction enzymes ...
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were carried out in a 20 µl volume containing Luna Universal qPCR Master Mix (New England Biolabs, Inc., Cat. no. NC1276266), F515 (GTGCCAGCMGCCGCGGTAA ...
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bioRxiv - Immunology 2022Quote: ... Libraries were size selected for cDNA target fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplification using Phusion DNA polymerase (New England Biolabs, Ipswich, MA, USA). The expression of each transcript was calculated according to the transcripts per million reads method ...
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bioRxiv - Microbiology 2021Quote: ... cDNA (2.5 μL) was amplified in two multiplexed PCR reactions using Q5 Hot-Start DNA High-fidelity Polymerase (New England Biolabs, Ipswich, MA, USA) using the following cycling conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... A 1524 bp cDNA fragment (encoding 508aa) was PCR amplified from bovine superficial zone articular chondrocyte cDNA using the Q5 High-Fidelity 2X Master Mix (NEB, cat. no. M0492S) and then cloned into the pCR®-Blunt vector (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... These constructs were synthesized as gBlocks (Integrated DNA Technologies) and PCR-amplified before transfection using Q5 polymerase (New England Biolabs, Cat No. M0491S). To generate the repair template ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... The TD-PCR protocol began with an annealing temperature of Tm + 10 °C for the first cycling phase (Tm calculated by NEB Tm Calculator tool). The annealing temperature was then decreased by 1 °C per cycle until the primers’ Tm was reached ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were PCR-amplified for 14 cycles and samples-specific dual indices (“NEBNext Multiplex Oligos for Illumina®”, E7600; New England BioLabs, Ipswich, USA) were attached to the fragments.
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bioRxiv - Microbiology 2021Quote: ... A 40 bp sequence homology was introduced in the two above mentioned fragments during the PCR and they were ligated with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs; catalog number E5520S). The whole cassette was then amplified with the end primer set ...
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bioRxiv - Immunology 2019Quote: ... Then sgRNA template for in vitro transcription (IVT) was prepared by PCR amplification of Phusion high fidelity DNA polymerase (NEB Biolabs, Ipwich, MA), the PCR mixture was cleaned up by PCR cleanup reaction (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... 500 ng total of the purified PCR products were mixed with 1 μl 10× NEBuffer 2 (New England Biolabs Inc., Beverly, MA, USA) and ultrapure water to a final volume of 9.75 μl ...
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bioRxiv - Biochemistry 2021Quote: ... or pET28a-Hisgp68-(T7pr_gp71-73) plasmids were assembled from two PCR-fragments using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2022Quote: ... 2004) PCR reactions were performed in MiniAmp Plus Thermal Cycler with Q5® High-Fidelity DNA polymerase (2000 U/mL) (NEB Ipswich, Massachusetts) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Primers specific for the region were designed using primer-BLAST and PCR amplification of the region was performed with Q5 DNA polymerase (New England Biolabs, Ipswich, Massachusetts, USA). PCR product was then sequenced by reverse primer ...
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bioRxiv - Synthetic Biology 2019Quote: ... Q5® High-Fidelity 2X Master Mix was used for long PCRs (Product M0492SNew England Biolabs 240 County Road Ipswich, MA 01938-2732). The correct gene products were confirmed by DNA sequencing (BaseClear BV ...
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bioRxiv - Pathology 2020Quote: ... DNA was visualized on 2% agarose gel and genotypes were determined based on PCR product length quantified using a DNA ladder (NEB N0556S, New England Biolabs Inc., MA, USA). Samples with a band at 743 bp were considered mutant (NntC57BL/6J) ...
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bioRxiv - Cancer Biology 2020Quote: ... Full-length TP53 and RB1 cDNA sequences from each of the samples were amplified by PCR using Phusion High-Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA). PCR products were electrophoresed on agarose gels ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR amplification was performed with 10 µl of the reverse transcriptase product in a 50 µl PCR mix containing 1 unit of Phusion High-Fidelity DNA polymerase (New England Biolabs, Évry-Courcouronnes, France), 1X HF Phusion buffer ...