Labshake search
Citations for New England Biolabs :
5701 - 5750 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... RNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). These samples were sequenced on the high-throughput sequencing platform (HiSeq2500 ...
-
bioRxiv - Immunology 2021Quote: ... sequencing libraries were generated using the NEBNext UltraTM RNA Library Prep Kit for Illumina (New England Biolabs), and the library quality was assessed on the Agilent Bioanalyzer 2100 system ...
-
bioRxiv - Molecular Biology 2021Quote: ... DNA sequencing libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB; E7645) according to the manufacturer’s instructions and quantified with KAPA Library Quantification Kit for Illumina (Kapa Biosystems) ...
-
Evaluation of the OsTIR1 and AtAFB2 AID systems for chromatin protein degradation in mammalian cellsbioRxiv - Molecular Biology 2021Quote: ... and selection cassette (either NeoR or HygroR) were assembled in one reaction using NEBuilder HiFi kit (NEB) in pMK289 backbone [3] ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from 1 million cells using the Monarch Total RNA Miniprep Kit (NEB, #T2010S). We prepared cDNA from 1μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA was reversely transcribed using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs #E7765S) as suggested by the manufacturer ...
-
bioRxiv - Molecular Biology 2021Quote: ... H3 and input) was processed with NEBNext Ultra II DNA Library Prep Kit (New England Biolabs #E7103S) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Libraries were generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7103, New England Biolabs). The IDT Unique Dual Index adapters and universal primers used were [AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC][AGATCGGAAGAGCGTCGTGTAGGGAAAGA GTGT] ...
-
bioRxiv - Microbiology 2021Quote: ... Site directed mutagenesis of plasmids were performed using Q5® Site-Directed Mutagenesis Kit (New England BioLabs) following manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... and site-specific mutations were introduced into DNA using the Q5 Site-Directed Mutagenesis Kit (NEB, USA) and custom-made primers ...
-
bioRxiv - Molecular Biology 2020Quote: Purified DNA or cDNA was subjected to NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). The DNA was resuspended in 25 μl ddH2O ...
-
bioRxiv - Plant Biology 2020Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
-
bioRxiv - Genomics 2020Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Prep Kit (New England Biolabs, Ipswitch, MA, USA), and quantified using an Agilent Bioanalyzer 2100 DNA 1000 kit (Agilent Technologies ...
-
bioRxiv - Neuroscience 2020Quote: ... The RNA sequencing library was generated using NEBNext® Ultra RNA Library Prep Kit (New England Biolabs) according to manufacturer’s protocols ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Specific DIG-labelled RNA probes were produced using HiScribe Sp6 and T7 in vitro transcription kits (NEB). The sequences of the probes are in the Supplementary Table 5 ...
-
bioRxiv - Immunology 2021Quote: ... The reaction was purified using a PCR clean-up kit according to manufacturer’s directions (NEB, Cat#: T1030S) or using ExoSAP-IT ...
-
bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription of RNA using ProtoScript II First Strand cDNA synthesis kit (New England BioLabs, NE, E6560L) following the manufacturer protocol ...
-
bioRxiv - Genetics 2020Quote: ... DNA libraries were then prepared by PCR amplification using NEBNext High-Fidelity PCR kit (New England Biolabs) in the presence of barcoded PCR primers (sequences provided in Ref ...
-
bioRxiv - Bioengineering 2019Quote: ... and libraries were constructed using an NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770). The libraries were quantified using a Qubit dsDNA HS Kit (ThermoFisher Scientific #Q32854 ...
-
The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Illumina paired-end libraries were constructed using the Ultra II RNA Directional kit (New England Biolabs, USA) and sequenced with an Illumina HiSeq v4 ...
-
bioRxiv - Pathology 2019Quote: ... Libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s instructions and index codes were added to attribute sequences to each sample ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... the libraries for Illumina whole-genome sequencing were prepared with NEBNext master mix kits (New England Biolabs), and the sequencing was done with Illumina HiSeq2000 (Mattila et al ...
-
bioRxiv - Developmental Biology 2019Quote: ... Guide RNAs were generated by PCR and transcribed using HiScribe T7 High-Yield RNA Synthesis Kit (NEB). 110-140 pg of guide RNA and 170 pg of cas9 mRNA per embryo was injected at one-cell stage into the cell ...
-
bioRxiv - Developmental Biology 2019Quote: ... F387F*fs mutation was generated in the vector pCDNA3 hGli3 using Q5 site directed mutagenesis kit (NEB) using primers Gli3 F387Ffs* Fwd CCAACACAGAGGCCTATTCC and Gli3 F387Ffs* Rev AAAGTTGGGGCAGGGTGG following the manufacturer’s instruction ...
-
bioRxiv - Synthetic Biology 2019Quote: ... PCR products with the expected size were column-purified with the Monarch PCR & DNA Cleanup Kit (NEB) and Sanger sequenced by GATC-Eurofins or Microsynth ...
-
bioRxiv - Genomics 2019Quote: DNA libraries were constructed using the NEBNext® Ultra™ II DNA Library Prep kit (NEB, USA) following manufacturer’s recommendations ...
-
bioRxiv - Biophysics 2019Quote: ... The RNA was synthesized in vitro using HiScribe T7 ARCA mRNA Kit (New England Biolabs, Ipswich, MA) or mMESSAGE mMACHINE T7 ULTRA Transcription Kit (ThermoFisher ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was performed using the Phusion High-Fidelity PCR Kit (New England Biolabs Inc, Ipswich, UK) and the final reaction contained 250 ng of template DNA ...
-
bioRxiv - Genomics 2019Quote: ... Sequencing libraries were prepared using a NEBNext Ultra II DNA Library Prep Kit (New England Biolabs; E7103). ATAC-seq was conducted as described in (Buenrostro et al. ...
-
bioRxiv - Genetics 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
-
bioRxiv - Genetics 2020Quote: ... corresponding to the point mutation in the SARS-CoV-2 genome (Q5 Site-directed mutagenesis kit, NEB). Site directed mutagenesis primers (Table S1 ...
-
bioRxiv - Genetics 2020Quote: The cDNA was generated by ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs, #E6560L). qPCR was performed toward eol-1 ...
-
bioRxiv - Genomics 2021Quote: ... and the manufacturer’s instructions were followed as outlined in the kit manual (New England BioLabs, Inc., USA). The library was sequenced across two Illumina HiSeq 2500 lanes with 2×150 bp paired-end sequencing at the Brigham Young University DNA Sequencing Center (Provo ...
-
bioRxiv - Genomics 2021Quote: The EM-seq libraries were prepared using the NEBNext Ultra II DNA library prep kit (NEB E7645) (using the NEBNext EM-seq adapter) ...
-
bioRxiv - Physiology 2021Quote: ... Libraries were prepared using NEBNext Ultra II Directional Library Prep Kit (New England Biolabs, Ipswich, MA, USA). Sequencing was performed on the NextSeq500 platform (Illumina ...
-
bioRxiv - Microbiology 2021Quote: ... Plasmid assembly was performed using Gibson assembly and the NEB HiFi assembly kit (NEB, New England, MA). The assembly mixture was transformed into E ...
-
bioRxiv - Microbiology 2020Quote: ... Specific gRNA and KOD PCR were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) on the pSAG1::Cas9-U6::sgUPRT vector (42 ...
-
bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with 9 cycles PCR amplification ...
-
bioRxiv - Molecular Biology 2021Quote: Samples with CT <25 were submitted to cDNA synthesis protocol using LunaScript™ RT SuperMix Kit (NEB) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: RNA-sequencing libraries were produced with NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) from hiPSC-derived astrocytes of ST ...
-
bioRxiv - Genomics 2021Quote: ... In Vitro Transcription (IVT) was performed with T7 high yield RNA synthesis kit (New England Biolabs, E2040S). The RNA from the IVT was purified using TRIzol first (Ambion ...
-
bioRxiv - Developmental Biology 2021Quote: ... Comparable total RNA quantities were used for reverse transcription with LunaScript RT SuperMix Kit (New England Biolabs). cDNA was amplified with Phusion polymerase for 30-36 cycles and resulting PCR products were separated on a standard 1% agarose gel next to a 100 bp ladder (NEB) ...
-
bioRxiv - Cell Biology 2020Quote: DNA libraries were generated using the NEBNext DNA Library Preparation Kit Ultra II (New England Biolabs, USA). Libraries were generated using 15ul of the ccfNA according to the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2020Quote: ... Site-directed mutagenesis was performed using Q5 Hot Start High Fidelity Kit from New England Biolabs (NEB). Vectors were transformed into E ...
-
bioRxiv - Biochemistry 2021Quote: ... All Pf-SUMO and Pf-Ubc9 mutants were generated using Q5 Site-Directed Mutagenesis kit (NEB #E0554S) in Pf-SUMO wild type backbone ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... cDNA libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and cDNA fragments (150 ∼ 200 bp in length ...
-
bioRxiv - Biochemistry 2021Quote: ... Mutations were introduced into this construct using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs), and proteins were expressed in Escherichia coli BL21 (DE3 ...
-
bioRxiv - Biochemistry 2021Quote: ... The PCR fragment and the plasmid (pOPINS3C) were assembled using the NEBuilder HiFi DNA Assembly kit (NEB), following the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2021Quote: ... the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) was used to generate the RNA libraries ...
-
bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA was depleted from 300 ng total RNA using the NEBNext rRNA Depletion Kit (NEB, #E6310L), and libraries were generated for next-generation sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...