Labshake search
Citations for New England Biolabs :
5851 - 5900 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... We then consecutively mutated the Y549A mutation using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with primer set ...
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bioRxiv - Synthetic Biology 2022Quote: ... The S222N mutant of AtADT212 was recreated by site-directed mutagenesis (Q5® Kit, NEB, Ipswich, MA). The empty vector was created by PCR-amplifying the pHyo182 backbone outside the start and stop codons of the AtADT2 ORF and ligating the ends ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA from U2OS cells were extracted by using Monarch Total RNA Miniprep kit (New England Biolabs) and treated with TURBO DNase (Thermo Fisher) ...
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bioRxiv - Plant Biology 2022Quote: ... the sequencing libraries were constructed with a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced on the HiSeq 4000 platform to obtain 150-bp paired-end data ...
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bioRxiv - Microbiology 2022Quote: ... the cDNA libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) following manufacture’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared with the NEBNext® Ultra II DNA Library Prep Kit for Illumina (NEB, E7645L) and single-end (1 x 75 bp ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and performing directed mutagenesis for each TFBS with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Primers used for deleting the TFBSs can be found in Table S6 ...
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bioRxiv - Immunology 2022Quote: ... Fragments were cloned into HpaI-digested pMSCVpuro using the Gibson Assembly Cloning Kit (New England Biolabs, E5510S). Plasmids were transformed into E ...
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bioRxiv - Synthetic Biology 2022Quote: ... whole genome sequencing libraries were generated using the NEBNext Ultra II FS DNA Library Prep Kit (NEB) and sequenced using an Illumina NextSeq 500/550 High Output Kit v2.5 (75 Cycles) ...
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bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were then indexed using the NEBNext Dual Index Kit (E7600S; New England Biolabs, Ipswitch, MA). Final cDNA libraries were analyzed by DNA ScreenTape Analysis on an Agilent 4200 TapeStation per the manufacturer’s protocol to determine library size ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was isolated from the cells using Monarch® Genomic DNA Purification Kit (New England Biolabs). DNA concentration was measured with Nanodrop One/OneC spectrophotometer (Thermo Fisher) ...
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bioRxiv - Plant Biology 2024Quote: ... Library preparations were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs; NEB). Libraries with different indices were multiplexed and sequenced on an Illumina HiSeq platform (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... Library preparations were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs; NEB). Libraries with different indices were multiplexed and sequenced on an Illumina HiSeq platform (Illumina ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were submitted to Novogene for library preparation (NEB Next® Ultra™ RNA Library Prep Kit) and paired-end (150bp ...
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bioRxiv - Genetics 2024Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... 20μL of DNA were used for libraries preperation using the NEBNext Ultra II DNA kit (NEB, #E7645). Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes ...
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bioRxiv - Synthetic Biology 2024Quote: ... The variants were created from the wild type plasmids using the Q5 Site-Directed Mutagenesis Kit (NEB) and transformed into MegaX cells made chemically competent (Mix & Go E ...
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bioRxiv - Physiology 2023Quote: ... and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Inc., Ipswich, MA, USA), with minor modifications to the manufacturers’ protocol (Kelley et al ...
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bioRxiv - Plant Biology 2024Quote: ... RNA sequencing was executed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... Whole cells were processed with the “NEBNext Single Cell/Low Input RNA Library Prep” kit (NEB #E6420) by following manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: Suitable primer pairs (Table S5) were designed utilizing the Q5 site-directed mutagenesis kit (New England Biolabs) to generate mutated amidases ...
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bioRxiv - Cancer Biology 2024Quote: ... and poly(A) mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation kit (NEB E7490L). The RNA library for direct RNA sequencing was prepared from ∼500 ng of poly-A RNA according to the Oxford Nanopore technology (ONT ...
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bioRxiv - Microbiology 2024Quote: ... The SA pathway was then introduced into pGW44 using the Gibson Assembly Cloning Kit (New England BioLabs), creating plasmid pJM455 ...
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bioRxiv - Microbiology 2024Quote: ... Genomic libraries were prepared using the NEBNext Ultra II FS library preparation kit (New England Biolabs, USA) according to the manufacturer’s instructions for MiSeq (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... 20-50 ng of purified RNA was processed by NEBNext rRNA Depletion Kit (New England BioLabs, Inc.) for removing ribosomal RNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified pools were subjected to PCR product purification by Monarch PCR and DNA Cleanup Kit (NEB). The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).
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bioRxiv - Cancer Biology 2024Quote: ... GAPF libraries were constructed with the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs), which employs fragmentase for DNA fragmentation ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were built with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer instruction ...
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bioRxiv - Immunology 2023Quote: ... Immunoprecipiated DNA was used to prepare libraries using NEBNext Ultra II DNA library prep kit (NEB E7645S). Libraries were sequenced with paired-end 50 on an Illumina Novaseq.
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bioRxiv - Molecular Biology 2024Quote: ... 3 uL RNase A and 1 uL ProtK all from the Monarch gDNA extraction kit (NEB, T3010). gDNA was extracted following the extraction kit’s protocol with the exception that 600 uL of gDNA binding buffer were added to 400 uL quenched reaction and added to the extraction column on two spins of 2 minutes at 1000g ...
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bioRxiv - Cell Biology 2024Quote: ... The ChIP libraries were constructed utilizing NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645L) and sequenced on an Illumina NovaSeq 6000 PE150 platform using the 150-bp pair-end sequencing parameters (Novogene ...
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bioRxiv - Cell Biology 2024Quote: ... RNA libraries were constructed utilizing the NEBNext® Ultra RNA Library Prep Kit for Illumina (NEB, E7530L) and sequenced on an Illumina NovaSeq 6000 PE150 platform using the 150-bp pair-end sequencing parameters (Novogene ...
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bioRxiv - Molecular Biology 2024Quote: ... and used for in-vitro transcription using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Finally ...
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bioRxiv - Microbiology 2024Quote: ... libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... A repair template plasmid was constructed using Gibson assemble (Gibson et al., 2008) (NEB Hifi assembly kit). The repair construct was made by joining the PfAMA1 coding region and ∼1.5 kb 5’ to the transcription start site amplified from 3D7 genomic DNA ...
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bioRxiv - Neuroscience 2024Quote: ... and effectors were cloned using the Gibson Cloning Assembly Kit (New England BioLabs, catalog no. NEB-E5510S). After cloning and sequencing ...
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bioRxiv - Immunology 2024Quote: ... cDNA was used to make libraries with the NEBNext Ultra II FS Library Preparation Kit (E6177S, NEB), using a five-fold lower reaction volume than instructed by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was then extracted and purified utilizing the Monarch Total RNA Isolation Kit (New England Biolabs), followed by triple DNase I (Promega ...
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
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bioRxiv - Neuroscience 2024Quote: ... and effectors were cloned using the Gibson Cloning Assembly Kit (New England BioLabs, catalog no. NEB-E5510S). After cloning and sequencing ...
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bioRxiv - Microbiology 2024Quote: ... DNA libraries were constructed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: cDNA libraries for sequencing were constructed using TruSeq RNA Library Prep Kits for Illumina® (NEB, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The oxidised PTC rRNA was subsequently purified from the reaction mixture with Monarch RNA Cleanup Kit (NEB) and immediately mixed with 150 nmol FTSC (300 eq. ...
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bioRxiv - Neuroscience 2024Quote: ... and cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560L). cDNA was amplified using primer pairs designed to either target two wildtype sequences on either side of the cryptic exon or one wildtype sequence and another sequence directly in the cryptic exon ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from samples using the Monarch® Total RNA Miniprep Kit (New England Biolabs, #T2010S) and cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, #E7530L). All libraries were prepared using manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA sequencing library preparation was performed using the NEBNext® DNA Library Prep Kit (New England Biolabs) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription reactions were performed using a LunaScript RT SuperMix Kit (#E3010L, New England Biolabs, Ipswich, MA) with 500 ng of total RNA ...