Labshake search
Citations for New England Biolabs :
5501 - 5550 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... and a library was prepared using the NEBNext Ultra II DNA library prep kit for Illumina (NEB) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Library preparation was conducted using an NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, Ipswich, MA), with a 12-cycle PCR enrichment ...
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bioRxiv - Genomics 2020Quote: ... Quantification of libraries was done by qPCR using the NEBNext library quant kit for Illumina (NEB, E7630) and library sizes were analyzed using the Agilent bioanalyzer 2100 HS DNA kit ...
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bioRxiv - Genomics 2020Quote: ... DNA was end-repaired using the NEBNext Ultra II End Repair/dA Tailing kit (New England Biolabs) and purified using AMPure XP beads ...
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bioRxiv - Genomics 2019Quote: ... Sequencing library preparation was performed using the NEBNext Ultra DNA library prep kit for Illumina (NEB, E7370S), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Libraries were generated using the NEBNext Ultra II Directional Library Prep Kit (New England Biolabs, Ipswich, MA) and subjected to sequencing (single-end 92x ...
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bioRxiv - Genomics 2020Quote: ... libraries were constructed with the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Illumina DNA sequencing libraries were prepared using the NEBNext Ultra II library preparation kit (New England Biolabs). 2 × 250 bp paired end sequencing reads were obtained using an Illumina HiSeq 2500 ...
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bioRxiv - Genomics 2020Quote: ... Libraries for Illumina sequencing were prepared following the NEBNext DNA Library Prep Master Mix kit (NEB E6040). Amplified libraries were size selected using a 2% agarose gel to extract fragments between 200 and 600 bp.
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bioRxiv - Genomics 2020Quote: ... RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep kit for Illumina (NEB). Agilent DNA High Sensitivity Chips (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs). Samples were indexed using NEBNext Multiplex Oligos (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... and then further purified using the Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA). A total of 250 ng RNA was used to synthesize cDNA with Reverse Tra Ace qPCR RT Master Mix (Toyobo Co. ...
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bioRxiv - Microbiology 2019Quote: ... we performed q-RT PCR using the Luna Universal One-Step RT-qPCR Kit from NEB (E3005), using the Applied BiosystemsΤΜ 7500 Real-Time PCR system ...
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bioRxiv - Developmental Biology 2020Quote: sgRNAs were then transcribed from templates using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) and purified using Megaclear Kit (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... The gltA complement plasmid was constructed using a Gibson Assembly Cloning Kit (New England Biolabs, Ipswich, MA). Briefly ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and whole genome sequencing libraries were prepared using the NEBNext Ultra II Library kit (New England Biolabs) according the recommended protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Transcriptomic sequencing of RNA was performed using NEBNext® rRNA Depletion Kit (New England Biolabs, Inc.; E6310S) and SMARTer Stranded Total RNA-Seq Kit - Pico Input (Clontech ...
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bioRxiv - Bioengineering 2019Quote: ... The NsiI site was then removed using the Q5 Site-directed mutagenesis Kit (New England Biolabs, E0554S). All sgRNA sequences for activation studies (Supplementary Table 1a ...
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bioRxiv - Neuroscience 2019Quote: ... and 24 hours of incubation GLuc activity was quantified using the BioLux Gaussia Luciferase Assay Kit (NEB) according to the manufacturer’s instructions with luminescent detection on a SpectraMax M5 Microplate Reader (Molecular Devices ...
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bioRxiv - Genetics 2020Quote: ... and the library generated with a NEB Next Ultra II DNA Library Prep Kit for Illumina (NEB). The quality of the library was checked using an Agilent 2100 Bioanalyzer (Agilent High Sensitivity DNA kit) ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted DNA fragments were purified on a Monarch PCR & DNA Cleanup Kit (#T1030S, New England BioLabs and eluted with 26 μL DNAase and RNAase free water ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: WGS was performed using a modified NEBNext UltraII for DNA Library Prep kit (E7645L, New England BioLabs). Briefly ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... We cloned the gBlock insert into our vector library using the Quick Ligation Kit (New England BioLabs) and transformed and prepared DNA from this plasmid library as detailed above ...
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bioRxiv - Genetics 2019Quote: ... and used as input into the NEB Ultra II Directional RNA library kit for Illumina (NEB E7760) in two batches ...
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bioRxiv - Genetics 2019Quote: ... Sequencing libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). The mRNA was selected by purifying polyA+ transcripts (NEBNext Poly(A ...
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bioRxiv - Biochemistry 2019Quote: ... Double stranded RNA reactions were performed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, #E2040S) and purified with the GeneJET RNA Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and a library was prepared using the NEBNext Ultra DNA library preparation kit (catalog number E7370L; NEB). Whole-genome sequencing of 150bp paired-end reads was generated on Illumina HiSeq2500 machine to a target coverage of 20X per sample ...
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bioRxiv - Microbiology 2019Quote: KLRC2 genotypes were determined by touchdown PCR using the Phusion High Fidelity PCR kit (New England Biolabs) using previously described methods40 ...
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bioRxiv - Genomics 2019Quote: ... Purified DNA sample was end-repaired (NEBnext ultra II end repair kit, New England Biolabs, MA, USA), cleaned up with 1x AMPure XP beads (Beckmann Coulter ...
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bioRxiv - Microbiology 2019Quote: Variants of UbiV and UbiU were constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturer’s specifications ...
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bioRxiv - Genomics 2019Quote: ... The second experimental replicate was prepared using the NEBNext Ultra II kit (New England Biolabs, Hitchin, UK) and sequenced using an Illumina NextSeq 500 platform ...
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bioRxiv - Plant Biology 2019Quote: ... ZymoResearch) and reverse-transcribed in the presence of random hexamers (LunaScript®RT SuperMix Kit, NEB Inc.) in 20 μl of total reaction volume containing 1 μg of total RNA and incubated at 55°C for 15 minutes following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 2013) with probes synthesized using components based on the NEBlot Phototope Kit (New England BioLabs, Cat# N7550) and detected using components based on the Phototope-Star detection kit (New England BioLabs ...
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bioRxiv - Evolutionary Biology 2019Quote: ... RNA sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530). NEBNext Poly(A ...
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bioRxiv - Microbiology 2019Quote: ... The in vitro transcripts were produced using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were then cloned using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB), following the recommended kit protocol and sequenced on a HiSeq2500 (Illumina).
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bioRxiv - Biochemistry 2020Quote: DNA was extracted from HLCs using the Monarch® Genomic DNA Purification Kit (New England BioLabs, T3010). A master mix was prepared using the Luna® Universal qPCR Master Mix (New England BioLabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and final RNA-seq libraries generated using the NEBNext Ultra II Directional RNA Library Prep kit (NEB, E7760 and NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Genomics 2019Quote: ... Fragmented DNA was used for library preparation using a NEBNext Ultra II Kit (NEB, Ipswich, MA USA) according to the manufacturer’s instructions for DNA end repair ...
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bioRxiv - Genetics 2021Quote: ... cDNA library was prepared with NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs), and the sequencing carried out using Illumina’s HiSeq-2500 sequencer with single-end mode and read length of 50 bp ...
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bioRxiv - Genetics 2020Quote: ... RNA was then reverse transcribed to cDNA using LunaScript RT SuperMix kit (Catalog # E3010L, New England BioLabs). w1118 flies might have partial or truncated transcripts of w+ ...
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bioRxiv - Genetics 2020Quote: ... Variants were generated using three-way Gibson cloning using NEB HiFi DNA Assembly Cloning Kit (NEB, E5520), using a NotI-AscI-digested and purified pENTR backbone (Thermo Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... Libraries were prepared from the resulting mRNA with the NEBNext® Ultra™ II kit (NEB #E7770S). sRNA seq libraries were generated with the NEBNext® Multiplex Small RNA kit (NEB #E7300S) ...
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bioRxiv - Cancer Biology 2021Quote: ... following the manufacturer’s protocol A160141 v01 for library amplification using the NEBNext Ultra II kit (NEB E7645S).
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bioRxiv - Cell Biology 2020Quote: ... each sample received a different barcoded adapter included in the NEBNext Multiplex Oligos kit (New England BioLabs) to allow for multiplexed sequencing ...
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bioRxiv - Immunology 2021Quote: ... The adaptor-ligated DNA fragments were indexed with the NEBNext Multiplex Oligos for Illumina kit (NEB, E7335). Adapted and indexed libraries were quantified using a KAPA Library Quantification Kit (Roche ...
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genomics 2020Quote: ... T7 transcription templates were transcribed using HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs). Reactions were cleaned up using the MEGAClear Transcription Clean-up Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Products of PCR amplification were subjected to KLD reaction using Q5 Site-Directed Mutagenesis Kit (NEB, USA) and transformed into NEB 5-alpha Competent E ...
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bioRxiv - Microbiology 2021Quote: ... Truncated versions of this minigenome were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) using the 3L5LEfull-Luc as a template.