Labshake search
Citations for New England Biolabs :
5951 - 6000 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L). In vitro transcription of pegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB) following instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (New England Biolabs® Inc). Cell free DNA (cfDNA ...
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bioRxiv - Cancer Biology 2023Quote: The WGS library was generated using NEB Next® Ultra™ DNA Library Prep Kit (NEB, USA), following the manufacturer’s recommended protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The tinR321N mutant expression plasmid was generated using the Q5 site-directed mutagenesis kit (New England Biolabs). The reporter plasmid was the Mp3D cardiac enhancer from the Multiplexin (Mp ...
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bioRxiv - Molecular Biology 2023Quote: ... The sgRNAs were transcribed in vitro by using a HiScribe T7 High Yield RNA Synthesis Kit (NEB), and purified by using TRIzol Reagent (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Cellular RNA was extracted from cell lysates using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed following the manufacturer’s instructions for Monarch Total RNA Miniprep Kit (New England BioLabs). Two step qPCR was carried out firstly by converting RNA into cDNA following the manufacturer’s instruction for LunaScript RT SuperMix Kit (New England BioLabs) ...
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bioRxiv - Microbiology 2023Quote: ... was constructed by assembling 3 DNA fragments using the NEBuilder HiFi DNA assembly kit (New England Biolabs). All 3 fragments were amplified from pTRL2-His-GrgA (28 ...
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bioRxiv - Genomics 2023Quote: ... The 125 sgRNAs were synthesized as a pool using the EnGen sgRNA Synthesis kit (New England Biolabs), and purified with the Monarch RNA Cleanup kit (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2023Quote: RNA samples were converted to cDNA using the Protoscript II First Strand cDNA Synthetsis Kit (NEB, E6560S) following the supplier’s standard protocol instructions ...
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bioRxiv - Microbiology 2023Quote: ... followed by library preparation with NEBNext Ultra DNA v2 Library Prep Kit for Illumina (New England Biolabs). Library preparation included 7 cycles of PCR amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng DNA was used to prepare libraries using the NEBNext Ultra II DNA kit (NEB E7645S) as described with modifications ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using NEBNext® μLtra™ DNA Library Prep Kit for Illumina (NEB, USA) using 1.0 μg DNA per sample ...
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bioRxiv - Microbiology 2023Quote: ... DNA samples were then used for library preparation using NEBNext UltraII DNA library prep kit (NEB #E7645L). Paired end sequencing of the libraries was performed using Illumina NextSeq 550 platform and atleast 3 million paired end reads were obtained for each sample ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was enriched from 400ng of total RNA using the NEBNext rRNA Depletion Kit v2 (NEB, # E7400X) to remove ribosomal RNA ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... Sequencing libraries were generated with NEBNext® UltraTM II DNA Library Prep Kit for Illumina (NEB, E7645S), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR-free libraries were prepared with the NEBNext UltraTM II DNA Library Prep Kits (New England BioLabs) according to supplier recommendations ...
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bioRxiv - Genomics 2023Quote: ... we did not measure the DNA concentrations) with a NEBNext Ultra II DNA kit (New England Biolabs) according to the manufacturer’s protocol and sequenced as 100-bp single-end reads on an Illumina NovaSeq 6000 instrument.
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bioRxiv - Genomics 2023Quote: ... Transcriptome libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, UK) according to the manufacturer’s instructions and sequenced with 150-bp paired-end sequencing using the Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... and adaptor ligation was performed on beads with the NEBnext Ultra II kit (New England Biolabs E7645). An optimal PCR cycle for final library amplification using NEBnext Ultra II Q5 was determined ...
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bioRxiv - Molecular Biology 2023Quote: ... All qRT-PCR reactions were performed with the Luna Universal One-Step RT-qPCR kit (E3005 NEB) according to manufacturer protocol with a few modifications ...
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bioRxiv - Synthetic Biology 2023Quote: ... suspended with nuclease-free water and directly used with HiScribe T7 RNA Polymerase kit (purchased from NEB). Full-length of transcribed product was purified via TBE:Urea PAGE gel ...
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bioRxiv - Plant Biology 2023Quote: ... and DNA libraries were prepared using NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs) and size selected (∼320 bp ...
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bioRxiv - Genomics 2023Quote: ... The UMI-4C sequencing library preparation was obtained using the NEBNext Ultra II Library Prep Kit (NEB). Library amplification was performed by nested PCR ...
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bioRxiv - Microbiology 2023Quote: ... Changes were introduced into the env genes using a Q5 site-directed mutagenesis kit (New England BioLabs). The presence of the desired mutations was confirmed by DNA sequencing.
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bioRxiv - Developmental Biology 2023Quote: ... Sequencing libraries were constructed with NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolabs). The cDNA was preamplified with KAPA HiFi HotStart ReadyMix ...
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bioRxiv - Microbiology 2023Quote: ... Two or three fragment assemblies were performed using TEDA or a HiFi DNA assembly kit (NEB #E2621) (29).
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bioRxiv - Microbiology 2023Quote: ... A RNAseq library was prepared by using a NEBNext® Ultra directional RNA library prep kit (NEB) and then sequenced on an Illumina NovaSeq 6000 sequencer ...
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bioRxiv - Microbiology 2023Quote: ... rLCMV-GP1-FLAG was generated by insertion PCR utilising Q5 site-directed mutagenesis kit (New England BioLabs) and FLAG primers ...
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bioRxiv - Plant Biology 2023Quote: ... RNA-Seq libraries were constructed using the NEB Next UltraTM RNA Library Prep Kit (NEB, Beverly, MA) and sequenced on a HiSeq 1500 platform.
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA was quantified using a Qubit 4 fluorometer (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... expression levels were determined using the Luna Universal One-Step RTqPCR kit (New England Biolabs catalog #E3005X) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and RpoD control were assessed using the Luna Universal One-Step qRT-PCR kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 20 µL H2O.Preparation of sequencing libraries was performed using NEBNext Ultra II kit (NEB) with 7 PCR cycles for library amplification ...
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bioRxiv - Neuroscience 2023Quote: ... . Libraries for Illumina short-read sequencing were prepared using the NEBNext Ultra II RNA library kit (NEB) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2023Quote: ... M Y229A and MT205D substitutions were generated using the Quick change directed mutagenesis kit (New England Biolabs), as recommended by the manufacturer.
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bioRxiv - Molecular Biology 2023Quote: ... and all constructions and intermediates were generated using a NEBuilder HiFi DNA assembly cloning kit (E5520S, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... A C-terminal HA tag was added using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). For microinjection ...
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bioRxiv - Genetics 2023Quote: ... The linearized Luc-shRunx2 vector and 1cisCXp promoter were further assembled together using Gibson Assembly Kit (NEB). A conceptual schematic of the gene circuits function is shown in Fig ...
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bioRxiv - Cell Biology 2022Quote: ... Libraries were generated using the NEBNext UltraTM RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) for the Illumina system following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Amplification of the library was performed with components of the NEBNext Ultra II DNA kit (NEB E7645S) and a NEBNext Multiplex Oligos set (e.g. ...
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bioRxiv - Biophysics 2023Quote: ... and/or insertions were achieved via site-directed mutagenesis (New England Biolabs Q5 site-directed mutagenesis kit) of the HH-pre-miR-31-HDV plasmid with primers listed in Table S10 ...
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bioRxiv - Cell Biology 2023Quote: ... SHORT and LONG reporters were generated by assembly (NEBuilder® HiFi DNA Assembly Cloning Kit, E5520S, NEB) of AURKA 5’UTR ...
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bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were generated for sequencing using the NEBNext Ultra II DNA Library Prep kit for Illumina (NEB) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were generated for sequencing using the NEBNext Ultra II DNA Library Prep kit for Illumina (NEB) as per the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Kits LSK109 and LSK110 were obtained from ONT and Companion Module NebNext from New England Biolabs (NEB). As a minor modification ...
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bioRxiv - Genetics 2022Quote: The construction of cavefish-tested pDEST_exorh:mGreenLantern and pDEST_exorh:mCherry was performed using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB E5520S). The original vector pDestTol2A2 (Tol2kit #394 ...
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bioRxiv - Developmental Biology 2022Quote: Complementary DNA (cDNA) encoding for Human CD36 (RC221976) was cloned using Hifi DNA assembly kit (NEB E5520S) into the pJFRC-MUH vector (Addgene ...