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Citations for New England Biolabs :
5601 - 5650 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: pACYC184Δtet5’ DNA extraction for supercoiling analysis was performed by using the Monarch®Plasmid Miniprep Kit (NEB). Cells were grown at the indicated temperature to an OD600 of 0.4 ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid pSF741 was used in a HiScribe T7 in vitro transcription kit (New England Biolabs, Ipswich, MA) to generate Venus mRNA ...
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bioRxiv - Microbiology 2019Quote: ... The DNA was isolated from the supernatant using the PCR clean-up kit (New England Biolabs®) and quantified using a nanodrop ...
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bioRxiv - Microbiology 2020Quote: cDNA was synthesized from 2 μg RNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB) per manufacture’s protocol ...
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bioRxiv - Cell Biology 2021Quote: The NEBNext® Ultra™ II DNA Library Prep Kit from NEB (New England Biolabs, Ipswich, MA) in the succeeding steps ...
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bioRxiv - Cell Biology 2021Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Library preparation was performed using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) or a TruSeq stranded mRNA sample prep kit (Illumina ...
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bioRxiv - Biophysics 2021Quote: ... The N501Y mutation was introduced by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs). Successful subcloning and mutation were confirmed by Sanger sequencing (Genewiz ...
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bioRxiv - Cell Biology 2020Quote: Point mutants were constructed as described in the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) and were confirmed via Sanger sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... We then carried out rRNA depletion with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat NEB # E6310S) using the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: A sequencing library was prepared using a NEBNext Ultra II Directional Kit (New England Biolabs, MA, USA). For the RIP-sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... YedK point mutants (H160E, H160Q, C2A/H160Q) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB). Mutant plasmids were sequence verified (GenHunter).
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bioRxiv - Biochemistry 2022Quote: ... The three fragments were assembled using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs, E2621) to generate pENTR1A-mitoLbNOX ...
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bioRxiv - Genomics 2022Quote: ... Barcoded libraries were made with NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) and sequenced on an Illumina NextSeq 2000 producing 100 bp paired-end reads.
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bioRxiv - Genomics 2022Quote: ... was added / activated and CUT&RUN enriched DNA purified using the Monarch DNA Cleanup kit (NEB #T1030S). 10 ng DNA was used to prepare sequencing libraries with the Ultra II DNA Library Prep kit (NEB #E7645S).
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bioRxiv - Molecular Biology 2022Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Biochemistry 2022Quote: ... The V40I mutation was created using a Q5 Site Directed Mutagenesis kit (New England Biolabs, MA, USA). The resulting plasmids ...
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bioRxiv - Genomics 2022Quote: ... and three hybrid males were constructed using the NEBNext Ultra II DNA kit (New England Biolabs, E7645S). RNAseq libraries were constructed from all reference tissues at the University of Montana using an Illumina TruSeq Stranded mRNA library kit (Illumina ...
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bioRxiv - Genetics 2022Quote: ... The codon for His4rK20 was changed to Ala using the Q5 Site-directed Mutagenesis kit (NEB E0554S). The same gRNA constructs in pCFD3 from Armstrong et al ...
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bioRxiv - Microbiology 2022Quote: ... Libraries for genome sequencing were prepared using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs) with random fragmentation to 350 bp and sequencing of 2×150 bp on a NovaSeq 6000 S4 flow cell (Illumina).
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bioRxiv - Molecular Biology 2022Quote: Site-directed mutagenesis of pSpike was performed using Q5® Site-Directed Mutagenesis Kit (New England Biolabs) and the forward and reverse primers given in Supplementary Table 5 ...
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bioRxiv - Microbiology 2022Quote: ... and mucI PCR products were ligated into pBGE using the Gibson Assembly Cloning Kit (New England Biolabs) to generate pmucG-C ...
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bioRxiv - Microbiology 2022Quote: ... which was then gel-purified using the Monarch DNA Gel extraction kit (New England Biolabs, MA, USA). S ...
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bioRxiv - Molecular Biology 2022Quote: The library was prepared according to NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB#E7645L) protocol ...
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bioRxiv - Microbiology 2022Quote: ... Freshly prepared supercoiled plasmid DNA was then isolated from overnight cultures using a Monarch miniprep kit (NEB) and then immediately subjected to restriction digestion with T7 endonuclease (NEB ...
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bioRxiv - Biophysics 2022Quote: ... The labeled primer was purified by G-25 microspin column (Cytvia) and Monarch DNA purification kit (NEB). The successful conjugation of three digoxigenin moieties was confirmed by the shift of the molecular weight using denaturing urea polyacrylamide gel electrophoresis ...
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bioRxiv - Systems Biology 2022Quote: ... cDNA was finally synthesized using the NEB Protoscript II First Strand cDNA synthesis kit (New England Biolabs) following the manufacturer’s instructions in parallel to control samples without the Reverse Transcriptase enzyme (-RT).
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bioRxiv - Biochemistry 2022Quote: ... sequencing libraries were prepared with a NEBNext Ultra II RNA Library Prep Kit (E7770, New England Biolabs) and samples were sequenced on an Illumina NovaSeq 6000 platform in 150bp paired-end reads ...
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Mechanism of the ANT-mediated transport of fatty acid anions across the inner mitochondrial membranebioRxiv - Biophysics 2022Quote: ... and aspartic acid 134 to serine using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Austria). The sequences were verified by both DNA and amino acid sequencing ...
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bioRxiv - Immunology 2022Quote: ... Monomeric versions of each CAR were created by using the Q5 Site Directed Mutagenesis Kit (NEB #E0554S) yielding two cysteine to serine point mutations in the CD8α hinge (Hennecke and Cosson ...
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bioRxiv - Immunology 2022Quote: ... Single nucleotide mutations were introduced using site-directed mutagenesis (SDM) (New England BioLabs, NEB Base Changer kit). Once confirmed ...
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bioRxiv - Developmental Biology 2022Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and sequenced using the HiSeq 50 cycle single-end reads protocol on the HiSeq 2500 system.
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bioRxiv - Neuroscience 2021Quote: ... for samples from library #1 and the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) for samples from library #3 ...
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bioRxiv - Neuroscience 2020Quote: Small RNA libraries were constructed from the RNA samples using NEBNext® kit (New England Biolabs, USA). Individually-barcoded libraries were mixed ...
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bioRxiv - Microbiology 2020Quote: ... and were later subcloned into pBJ5 vector using the NEBuilder HiFi DNA assembly kit (New England Biolabs). To amplify mM1 ...
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bioRxiv - Genomics 2020Quote: ... Second strand synthesis was performed using the NEBNext Ultra II second strand synthesis kit (NEB, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using Luna® Universal One-Step RT-qPCR kit (New England Biolabs; #E3005L). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample52 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’-phosphorylated and dA-tailed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Samples were ligated to adaptor oligos for multiplex sequencing (NEB ...
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bioRxiv - Genetics 2020Quote: ... Sequencing libraries were generated using NEBNext® small RNA Sample Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Bioengineering 2021Quote: ... RNA sequencing library preparation was done using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and then processed using NEBNext Ultra II Directional Library Prep Kit for Illumina (#NEBE7760L; New England Biolabs) for the creation of cDNA libraries ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared following NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs; #E7420) and Agencourt AMPure XP Beads (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from extracellular tachyzoites using Monarch® Genomic DNA Purification Kit (New England BioLabs). Presence of GRX1-based sensors was confirmed using primer pair 7/8.
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bioRxiv - Neuroscience 2020Quote: ... DNA from 2 biological replicates was purified with Monarch PCR & DNA Cleanup Kit (Cat. no. T1030, NEB). Library preparation ...
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bioRxiv - Microbiology 2020Quote: ... Polyadenylated transcript enrichment and strand specific library preparation was completed using NEBNext Ultra II mRNA kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Libraries were prepared using the Next Single Cell / Low Input RNA Library Prep Kit (New England Biolabs). The quality and quantity of the final libraries were assessed with the TapeStation D5000 Assay (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: Libraries were prepared using the Next Single Cell / Low Input RNA Library Prep Kit (New England Biolabs). The quality and quantity of the final libraries was assessed with the TapeStation D1000 Assay (Agilent Technologies ...
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bioRxiv - Immunology 2020Quote: ... white colonies were picked and the plasmids were isolated using the Monarch® Plasmid Miniprep Kit (NEB). To verify that the picked colonies contain an insert of the correct size ...
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bioRxiv - Microbiology 2021Quote: Prepared libraries were quantified using NEBNext® Library Quant Kit for Illumina® E7630L (New England BioLabs) and pooled with volumes adjusted to normalize concentrations and provide for ∼1,000-fold genomic coverage for population samples (20–30 samples per HiSeq lane depending on the genome size ...