Labshake search
Citations for New England Biolabs :
5451 - 5500 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Further modifications were performed by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB). All modifications to the 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO plasmid (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E7645) following manufacture’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and subsequently replaced with the tailed rpoS DNA fragment using the HiFi DNA assembly kit (NEB). Following the assembly reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR product was cloned into the pCDF4 vector using the Gibson Assembly kit (NEB) and the resulting plasmid was then used to generate ATG4-gRNA transgenic flies via injection into the y1 ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesised according to the protocols of the LunaScript RT Supermix Kit (New England Biolabs) and for qPCR amplification ...
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bioRxiv - Plant Biology 2020Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB), following the manufacturer’s recommendations and sequenced on Illumina Hiseq platform and 150 bp paired-end reads were generated.
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bioRxiv - Microbiology 2020Quote: ... and ftsH target sequences was carried out in vitro using a Q5 mutagenesis kit (New England BioLabs) with specified primers (S1 Table) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was collected at 9 h post-IFN treatment using Monarch Total RNA Miniprep Kits (NEB). RNA was prepped for RNA sequencing submission using the NEBNext Poly(A ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR reactions were purified with Monarch DNA Cleanup Kit columns (T1030; New England Biolabs; Ipswich, MA, USA) per the manufacturer’s protocol and eluted in 20 µL nuclease-free water.
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bioRxiv - Genomics 2021Quote: ... 1μl RT Adapter (110nM; ONT Kit) and 1.5μl T4 DNA Ligase (2M U/ml New England BioLabs). The ligation reaction was carried out for 10min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... ΔN1/2/3 and ΔDAD mutants were generated by Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) with HOXB13 WT or NCOR1 WT as a template ...
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bioRxiv - Biophysics 2020Quote: ... FR-Q and FR-MQ were made using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with pBad-FR and pBad-FR-M ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was transcribed to cDNA using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB E7530). RNA sequencing was performed using single-end 75 bp reads on an Illumina NextSeq500 ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA-seq libraries were constructed using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) with size selection for ~200 bp insert DNA ...
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bioRxiv - Cell Biology 2020Quote: ... Site directed mutagenesis to generate GATA4 V266M was performed with the Q5 Site-Directed Mutagenesis Kit (NEB) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Sequencing library preparation was performed using the NEBNext Ultra DNA library prep kit for Illumina (NEB, E7370S), following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: Sequencing library was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB E7770S) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... and libraries were prepared using NEBNext UltraII Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genetics 2021Quote: ... Libraries were then cloned using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB), following the recommended kit protocol and sequenced on a NovaSeq 6000 – SR100 (Illumina).
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bioRxiv - Genetics 2020Quote: ... We performed RNA purification and genomic DNA removal using the Monarch Total RNA Miniprep Kit (T2010S, NEB) following manufacturer’s instructions for purifying TRIzol-extracted samples ...
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bioRxiv - Microbiology 2021Quote: ... RT was performed using 1 μg of RNA using LunaScript™ RT SuperMix Kit (New England BioLabs) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Total blood RNA from DBS was extracted using Monarch Total RNA Miniprep Kit (New England Biolabs, USA) with a modified protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... pUHES:UmPit2+SP:mCherry with its natural signal peptide was generated via NEBuilder HiFi DNA Assembly Cloning Kit (NEB) by combining PCR-amplified UmPit2+SP:mCherry using primers 2127 + 2128 and PCR-amplified pUHESdest using primers 2125 + 2126 without UhAvr1 signal peptide ...
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bioRxiv - Microbiology 2019Quote: ... The sequencing library was prepared using a NEBNext Ultra II RNA library prep kit (New England BioLabs) and quantified using a NEBNext Library Quant kit for Illumina (New England BioLabs) ...
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bioRxiv - Microbiology 2019Quote: ... Library preparation was carried out using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) following manufacturers guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... This is then followed by second- strand synthesis and NEB ultra-directional RNA sequencing kit (NEB #E7760).
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bioRxiv - Molecular Biology 2021Quote: ... Derivatives of pEAI423 containing mutations in EXO1 were constructed with the Q5 mutagenesis kit (New England Biolabs) using pEAI423 as template and the oligonucleotides shown in Table S7 ...
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bioRxiv - Developmental Biology 2021Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and sequenced using the HiSeq 50 cycle single-end reads protocol on the HiSeq 2500 system.
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample [49] ...
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bioRxiv - Evolutionary Biology 2019Quote: ... RNA sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530). NEBNext Poly(A ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... were introduced in full-length ezh2 cDNA by the Q5® Site-Directed Mutagenesis Kit (NEB; E0544S). Resulting plasmids were validated by Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by library preparation using the NEBNext Ultra II library kit (New England BioLabs Ltd, Hitchin, UK). The final library pools were generated and sequenced on the Illumina X ten platform (Illumina ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Libraries were prepared using the NEBNext® Ultra™ II DNA Kit (New England Biolabs, Massachusetts, USA). Samples with high molecular weight DNA (>1000 bp ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro synthesized cRNA was purified by using Monarch RNA cleanup kit (New England Biolabs, Ipswich, MA) and subjected to first strand ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced on a HiSeq 4000 system (Illumina ...
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bioRxiv - Immunology 2022Quote: ... followed by library preparation using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were generated with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) using 250 ng of input genomic DNA ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) as per the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2022Quote: ... CPB containing a C-terminal His6-tag was generated using Q5® site-directed mutagenesis kit (NEB), pET-19b_His6_CPB as template and primer pair #1 and elongation time of 4 min to delete the N-terminal His6 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760), with cDNA generated from fresh RNA samples using 500ng of RNA and 10 cycles of PCR ...
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bioRxiv - Molecular Biology 2020Quote: Purified DNA or cDNA was subjected to NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). The DNA was resuspended in 25 μl ddH2O ...
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bioRxiv - Molecular Biology 2021Quote: ... This is then followed by second-strand synthesis and NEB ultra-directional RNA sequencing kit (NEB #E7760).
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bioRxiv - Molecular Biology 2021Quote: sgRNAs were produced by in vitro transcription using the HiScribe T7 High Yield RNA synthesis kit (NEB) with PCR amplified gBlocks (IDT ...
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bioRxiv - Molecular Biology 2020Quote: Libraries were prepared for sequencing using the NEBNext Ultra II library preparation kit (New England Biolabs, USA) according to manufacturer’s protocol and barcoded using NEXTflex barcodes (BIOO Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... pGEMHE plasmids were linearized and 5’-capped mRNA was synthesised with T7 polymerase (NEB HiScribeT7 ARCA kit) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ChIP-Seq libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645) and barcoded multiplex libraries were sequenced using Illumina MiSeq platform ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutations were introduced into UNC-45B wild-type using the Q5® Site-Directed Mutagenesis Kit (NEB). Recombinant protein expression was induced in BL21 DE3 when optical density (OD600 ...