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Citations for New England Biolabs :
5551 - 5600 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Eluted DNA was subjected to library construction using NEBNext Ultra II DNA Library Prep kit (NEB, Cat#E7645S). Paired-end 150bp reads were obtained from a Illumina NovaSeq system ...
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bioRxiv - Biochemistry 2024Quote: ... The NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II RNA Library Prep Kit (NEB BioLabs) were used to prepare RNA libraries ...
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bioRxiv - Biochemistry 2024Quote: ... The NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II RNA Library Prep Kit (NEB BioLabs) were used to prepare RNA libraries ...
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bioRxiv - Genomics 2024Quote: In vitro transcription was performed with the NEB HiScribe® T7 High Yield RNA Synthesis Kit (NEB#E2040) in 20 µl of total volume ...
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bioRxiv - Biochemistry 2024Quote: ... The total RNA were extracted using a Mornarch® RNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacture’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... amplified plasmids were purified using PCR & DNA cleanup kit (Monarch) treated with T4 polynucleotide kinase (New England Biolabs) during 1 h at 37°C and ligated using T4 DNA ligase (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... The qPCR reactions were prepared following the standard protocol for the Luna kit (New England Biolabs, Ipswich, MA). Oligonucleotide sequences are in Table S2.
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bioRxiv - Genomics 2024Quote: ... Components were used from the LSK114 kit along with several buffers and enzymes from New England Biolab (NEB) following the LSK114 protocol from Oxford Nanopore ...
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bioRxiv - Microbiology 2024Quote: ... The reverse transcription was performed with ProtoScript® II First Strand cDNA Synthesis Kit (E6560S, New England Biolabs) using the optimised Random Primer Mix provided by the manufacturer ...
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bioRxiv - Immunology 2024Quote: Cloning of miBds into pMB-LINK was performed using NEBridge® Golden Gate Assembly Kit (BsmBI-v2) (NEB) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... the sequencing library was prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). The genomic sequences were obtained using AVITI sequencer (Element Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... Cut vector DNA was purified with a PureLink PCR kit and treated with T4 DNA polymerase (NEB M0203) in the presence of 0.25 mM dGTP (pNIC-CTHF ...
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bioRxiv - Biochemistry 2024Quote: FnCas9 Y794A mutant was cloned using Q5 Hot Start Hi-Fidelity polymerase and KLD kit (New England Biolabs). The mutated sequences were verified by Plasmidsaurus and purified in the same manner as WT FnCas9.
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bioRxiv - Biophysics 2024Quote: Single point mutants were prepared in house using the Q5® Site-directed mutagenesis kit (New England Biolabs) and primers whose list can be found in Table S2.
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bioRxiv - Biophysics 2024Quote: ... Molecular biology was carried out using Gibson Assembly and Q5 site-directed mutagenesis kit (New England Biolabs, NEB). The protein was expressed in Tuner (DE3 ...
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bioRxiv - Biophysics 2024Quote: ... Molecular biology was carried out using Gibson Assembly and Q5 site-directed mutagenesis kit (New England Biolabs, NEB). The protein was expressed in Tuner (DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... The gRNA was synthesized using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050S) in 20 ul reactions containing 6 μl Milli-Q water ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for PCR used are as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... and library preparation was performed with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770S). Indices used were the NEBNext Multiplex Oligos for Illumina (NEB #E7335 ...
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bioRxiv - Bioengineering 2024Quote: ... DNA fragments were purified by agarose gel electrophoresis and subsequent purification using Monarch DNA Gel Extraction Kit (NEB).
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bioRxiv - Cell Biology 2024Quote: ... RT-qPCR was performed using the Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs), on a BioRad C1000 CFX96 touch screen thermal cycler ...
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bioRxiv - Cell Biology 2024Quote: ... and equal amounts of RNA were reverse transcribed using ProtoScript First Strand cDNA Synthesis Kit (New England Biolabs). Semiquantitative PCR was conducted using a StepOnePlus System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were prepared for sequencing with the RNA-seq NEB Next Ultra RNA Library Prep Kit (7530, NEB). Samples were sequenced on an Illumina Novaseq 6000 platform.
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bioRxiv - Biochemistry 2024Quote: ... Reverse transcription and RT-qPCR analysis was performed by Luna universal one-step RT-qPCR kit (NEB # E3005L) with the primers listed in Table 4.
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bioRxiv - Biochemistry 2024Quote: ... the genes were cloned into the vector backbone using the NEBuilder Hi-Fi Assembly Kit (New England Biolabs). NatBURP1 was cloned using primers (NatBURP1-For ...
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bioRxiv - Bioengineering 2024Quote: ... which was then used for plasmid preparation via the Monarch Plasmid Miniprep Kit (New England Biolabs, Ipswich, USA). Purified plasmids were used for transformation of C ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (NEB, cat. no. E7805). Libraries of around 300 bp size were sequenced using the Illumina HiSeq 2500 system (paired-end ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments were gel purified (Macherey Nagel Gel and PCR Cleanup Kit) and ligated with T4 DNA ligase (NEB). The ligation reaction was then transformed into C3040 cells and selected on LB+Amp agar plates ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were enriched for microbial DNA using the NEBNext Microbiome Enrichment Kit (New England BioLabs, Cat: #E2612L), removing the methylated host DNA ...
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bioRxiv - Cancer Biology 2024Quote: ChIP-seq libraries were prepped using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs E7645S) with NEBNext Multiplex Oligos Index Primers Sets for multiplexing ...
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bioRxiv - Cancer Biology 2024Quote: ... Mutant ADAT2 E73A was produced by directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Mutagenesis primers are detailed in Table S1 ...
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bioRxiv - Developmental Biology 2024Quote: The PCR procedures followed the protocol provided with the Q5 high fidelity DNA polymerase kit (cat# M0491, NEB) with thermal cycling conditions ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by in vitro transcription using using HiScribe® T7 ARCA mRNA Kit (New England Biolabs, Ipswich, MA). To overexpress METTL7A ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we extracted DNA using the Monarch Genomic DNA Purification Kit (New England Biolabs GmbH, Frankfurt am Main, Germany). For sequencing on the PacBio Sequel Ile platform (Pacific Biosciences Inc. ...
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bioRxiv - Cell Biology 2024Quote: ... The libraries were then prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, BE7645L) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Genetics 2024Quote: ... Two micrograms of total RNA were used for first-strand synthesis using the LunaScript RT SuperMix Kit (NEB). Gene expression was analyzed with quantitative real-time PCR (qPCR ...
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bioRxiv - Genetics 2024Quote: MBD-affinity purification was performed using the Epimark Methylated DNA Enrichment kit manufactured by NEB (Cat no. E2600S). The protocol was carried out as per the manufacturer’s guidelines ...
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bioRxiv - Genetics 2024Quote: ... (Sacramento, CA, USA) for library construction (NEBNext Ultra RNA Library Prep Kit, New England Biolabs, Ipswich, MA, USA) from 1 µg total RNA and Illumina sequencing using the NovaSeq 6000 Sequencing System ...
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bioRxiv - Evolutionary Biology 2024Quote: Libraries were generated using the NEBNext Ultra II DNA Library Prep Kit (PCR Free Library) (New England Biolabs). Sequencing was performed in the NovaSeq 6000 System (Illumina).
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bioRxiv - Cell Biology 2024Quote: ChIP-seq DNA libraries were prepared with NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: TT-seq libraries was prepared with NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB, E7760) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Metagenomic libraries of each individual were prepared with NEBNext Ultra II DNA Library Prep Kit (BioLabs, New England) and sequenced on Illumina HiSeq 2500 in a Rapid Run mode (2x250bp) ...
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bioRxiv - Synthetic Biology 2021Quote: ... total DNA from one clone was isolated using the Monarch Kit for HMW DNA Extraction from Bacteria (NEB#T3060) and confirmed by Nanopore sequencing.
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative RT-PCR was performed using Luna Universal One-Step RT-qPCR kit (New England Biolabs, Ipswich, MA, USA) according to manufacturer’s protocol using Agilent Mx3000P instrument ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... Renilla luciferase gene and Firefly luciferase gene were in-vitro transcribed using HiScribe T7 RNA Synthesis Kit (NEB Biolabs). After purified with Monarch RNA Cleanup Kit (NEB Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Adaptors were removed from the 3’ end of reads using cutadapt (Martin ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated using Invitrogen PureLink RNA Mini Kit following manufacturer’s instructions and treated with DNase (New England Biolabs). Biological triplicate samples were submitted to DNA Technologies and Expression Analysis Core at UC Davis Genome Center for the generation of 3’Tag RNA-Seq libraries ...
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bioRxiv - Microbiology 2020Quote: Site-directed mutagenesis was performed on pBPVP1456 with a Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) and primers listed in Table 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-Seq libraries (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, New England BioLabs, Catalog No. E7760) were produced from 16 samples according to the manufacturer’s protocol using 200 ng total RNA ...