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Citations for New England Biolabs :
5801 - 5850 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were prepared using the NEBNext Ultr II Directional RNA Library Prep Kit for Illumina (New England Biolabs). After indexing ...
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bioRxiv - Biophysics 2021Quote: DNA assembly one-step multiple fragment cloning technique (NEBuilder HiFi DNA assembly cloning kit, E5520, New England Biolabs, MA). We ligated the assembled products into a pXS2 plasmid [84] using the pXS2.Pex13.2 [85] plasmid (gift from Meredith Morris ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were assembled by Gibson assembly using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, USA).
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bioRxiv - Synthetic Biology 2022Quote: ... The RNA library was constructed using the NEBNext® Ultra II RNA Library Prep Kit for Illumina (NEB, USA) and sequenced using the Illumina HiSeq X Ten platform.
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bioRxiv - Neuroscience 2022Quote: ... RNA-seq libraries were generated from total RNA using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). Paired-end sequencing (2 x 75 base-pair reads ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified DNA was analysed using ChIP-qPCR and cChIP-seq libraries for both ChIP and input samples were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina following the manufacturer’s guidelines (NEB).
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bioRxiv - Molecular Biology 2022Quote: ... cChIP-seq libraries for both ChIP and input samples were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina following manufacturer’s guidelines (NEB).
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted and isolated from the retinas using the Monarch Total RNA Miniprep Kit (New England Biolabs) and genomic DNA was removed using RNase-free DNase (Monarch) ...
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bioRxiv - Genetics 2022Quote: ... and the HiFi reaction performed as per the manufacturer’s protocol using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB). Constructs were verified by a Sacll and Clal digest followed by Sanger sequencing.
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bioRxiv - Developmental Biology 2022Quote: ... diluted to 20nM and further quantified using the NEBNext Library Quant Kit for Illumina (New England Biolabs, MA, USA). Samples were pooled and diluted to 4nM and run on an Illumina Miseq by the Bioinformatics ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription of 10 µl RNA was performed using LunaScript RT Supermix Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... using NEBNext® Ultra(tm) II FS DNA Library Prep Kit for Illumina (New England Biolabs, Cat. No. E7805), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA) and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... followed by sequencing library preparation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Single-end 65-bp reads were sequenced by Illumina HiSeq-2500 system ...
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bioRxiv - Evolutionary Biology 2022Quote: ... all PCR products from cDNA and gDNA were cloned into the pMiniT 2.0 Vector using the NEB cloning kit (New Englands BioLabs) and sequenced to ensure sequencing of a single DNA molecule.
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bioRxiv - Microbiology 2022Quote: ... and the fucI PCR product was cloned into the plasmid using the NEBuilder HiFi DNA Assembly Kit (NEB; E5520S), followed by transformation into the E ...
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bioRxiv - Cancer Biology 2022Quote: The same sets of RNA samples were reverse transcribed using Protoscript II First Strand cDNA Synthesis Kit (NEB #E6560S). cDNA was measured using a QuantStudio 7 Flex Real-time PCR machine (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... Strand-specific RNA-seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, Ipswich, MA) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... RNA sequencing libraries were produced by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L). Quantification of the library was performed using a dsDNA HS Assay Kit and Qubit (Molecular Probes ...
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bioRxiv - Genomics 2022Quote: ... Illumina libraries were constructed using a NEBNext Ultra™ II DNA Library Prep Kit (New England Biolabs, Cat#: E7645). Libraries were quantified using a Bioanalyzer DNA 1000 chip (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... strand-specific RNA-seq libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB; E7420S) with library amplification specifically modified to accommodate the high AT content of P ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then transcribed from the NsiI-linearised plasmids with the HiScribe™ T7 ARCA mRNA Kit (NEB E2065S) or HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (NEB E2080S ...
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bioRxiv - Plant Biology 2022Quote: ... The NEBNext Ultra II RNA Library Prep with Sample Purification Beads kit (New England BioLabs, Inc., Ipswich, MA, USA) was used for library preparation and libraries were sequenced in two lanes on the 50 bp single-read Illumina HiSeq 4000 platform at the California Institute for Quantitative Biosciences (QB3 ...
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bioRxiv - Cell Biology 2022Quote: ... and recombined into and SphI digested pRS316-HCM1*SphI using the NEBuilder HiFi DNA assembly kit (New England Biolabs). Plasmids were then transformed into NEB 10-beta Competent E ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 9 cDNA libraries were prepared with an NEBNext Ultra II RNA Library Prep kit (NEB, cat:E7770S) and sequenced with an Illumina HiSeq4000 ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 8 cDNA libraries were prepared with an NEBNext Ultra II RNA Library Prep kit (NEB, cat:E7770S) and sequenced with an Illumina HiSeq4000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Targeting constructs with appropriate homology arms were generated by Gibson assembly using the Gibson Assembly Master Mix kit (NEB). Targeting constructs were designed such that Cas9 recognition sites were disrupted by the presence of the LoxP sites ...
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bioRxiv - Pathology 2020Quote: ... sequencing libraries were constructed with NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and libraries were sequenced using Illumina Hiseq 4000 platform (Illumina) ...
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bioRxiv - Epidemiology 2020Quote: ... cDNA was produced from viral RNA using random hexamers and the Protoscript II First Strand cDNA synthesis kit (NEB). The genome was amplified using a multiplex PCR scheme designed to produce overlapping 500bp amplicons across the whole coding region of the recent South American genotype I outbreak clade (8,18) ...
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bioRxiv - Molecular Biology 2020Quote: ... on an Agilent mx3005p instrument using New England Biolabs NEBNext® Library Quant Kit for Illumina (NEB, cat#E7630S). For each amplicon library ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... Leishmania DNA (0.0017 ng/μL) was then enriched from the human DNA (25ng/μL) using NEBNext Microbiome DNA Enrichment Kit (NEB) according to manufacturer instructions ...
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bioRxiv - Genetics 2021Quote: ... and library preparation was conducted using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10x diluted T7 RNA Polymerase mix (HiScribe™ T7 High Yield RNA Synthesis Kit, New England Biolabs, USA). After linear amplification ...
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bioRxiv - Microbiology 2021Quote: ... and RNA-seq libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) as per manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10x diluted T7 RNA Polymerase mix (HiScribe™ T7 High Yield RNA Synthesis Kit, New England Biolabs, USA). After linear amplification ...
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bioRxiv - Plant Biology 2020Quote: ... 34 µL DNase-free water was added to 16 µL precipitated DNA and adaptors were ligated to DNA fragments according to the manufacturer’s instructions (NEBNext Multiplex Oligos for Illumina kit NEB). After ligation ...
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bioRxiv - Pathology 2021Quote: ... Fragmented PCR products were repaired and dA-tailed using the NEBNext End Repair/dA Tailing kit (New England Biolabs) according to the manufacturer’s instructions and subsequently barcoded with Illumina-compatible ...
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bioRxiv - Molecular Biology 2021Quote: ... Adaptor ligation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645, New England Biolabs), and library pre-amplification was performed by 9 cycles of PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... 2017) using primers designed by NEBaseChanger and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs. Cat. No.: E0554S). The mutations were confirmed by DNA sequencing.
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bioRxiv - Microbiology 2020Quote: ... THUNDERBIRD Probe qPCR Master Mix (Toyobo) for MERS-CoV and Luna Universal Probe One-Step RT-qPCR Kit (NEB) for SARS-CoV-2.
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bioRxiv - Microbiology 2020Quote: ... The Cas9/gRNA expression construct pTREX-n-Cas9 was first modified using the Q5 mutagenesis kit (NEB, Ipswich, Massachusetts), primers 5’-CCCAAAAAGAAAAGGAAGGTTGATTAGAAGCTTATCGATACCGTCGAC-3’ and 5’-GTCCTCGACTTTTCGCTTCTTTTTCGGGTCGCCTCCCAGCTGAGA-3’ ...
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bioRxiv - Genetics 2020Quote: ... DNA that migrated between the 400 and 600 bp was extracted using Monarch DNA Gel Extraction Kit (NEB T1020L). The pooled library DNA concentration was determined using Qubit dsDNA BR Assay Kit (ThermoFisher Scientist Q32853) ...
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bioRxiv - Plant Biology 2020Quote: ... RNA libraries were produced using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Each library was barcoded and sequenced in one lane of an Illumina HiSeq 2500 ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA for RNA and PARE sequencing was obtained by purification with the NEB mRNA isolation kit (New England Biolabs). RNA for sRNA library preparation was enriched with the mirVana miRNA Isolation Kit (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Strand-specific RNA-seq library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, Ipswich, MA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: sgRNAs were generated by in vitro transcription using the Hiscribe T7 high yield RNA synthesis kit (New England Biolabs). Purified sgRNA (0.5 μg ...
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bioRxiv - Evolutionary Biology 2021Quote: ... see Supplementary Data 6) and cloned into pDHFR vector provided in the PURExpress In Vitro Protein Synthesis Kit (NEB). The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA-seq libraries were constructed by NEBNext Ultra II RNA Library Prep kit (NEB, Cat: E7775, E7335, E7500) and sequenced by Illumina NextSeq 500 system (Duan et al. ...
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bioRxiv - Genomics 2021Quote: ... Libraries were constructed using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs, Frankfurt am Main, Germany) following the manufacturer’s description ...
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... ERK7-mAID-HA (TGGT1_233010) was obtained by transfecting a gRNA obtained by Q5 site-directed mutagenesis kit (New England Biolabs) on the pSAG1::Cas9-U6::sgUPRT vector (Shen et al ...