Labshake search
Citations for New England Biolabs :
5401 - 5450 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... and RNA-seq libraries were constructed using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: DNA oligos of sgRNAs and x-gRNAs were designed according to the EnGen sgRNA Synthesis Kit (NEB #E3322) to add 5’-T7 RNA polymerase promoter sequence and 3’-Cas9 crRNA sequence and were purchased from Integrated DNA Technologies IDT then resuspended to a stock concentration of 100 μM ...
-
bioRxiv - Biochemistry 2022Quote: ... -R98E/E127A) were generated by introducing point mutations using the Q5 site-directed mutagenesis kit (New England BioLabs), following manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... All DNA fragments were fused in pCR2.1-TOPO (genome integration cassette) or pGRB2.0 (rescue plasmids) vectors using a Gibson assembly kit (New England Biolabs) and validated by sequencing ...
-
bioRxiv - Microbiology 2023Quote: ... Subsequently the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) was used to generate the RNA libraries ...
-
bioRxiv - Cell Biology 2023Quote: ... ChIP libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina per manufacturer’s instructions (NEB). Sequencing was carried out on Illumina’s NextSeq 500 platform to produce 75 bp single-end reads at a minimum of 50 million reads per sample ...
-
bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were generated using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s recommendations ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA-seq library was constructed with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB; E7765) through the polyA mRNA workflow ...
-
bioRxiv - Cell Biology 2023Quote: ... cDNA or genomic sequence of each gene was amplified by Phusion High-Fidelity PCR kit (New England BioLabs), cloned to pDonor 221 ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK293 libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat. #E7770) or NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (#E7760) ...
-
bioRxiv - Genomics 2022Quote: ... Three library construction were performed with the NEBNext DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA). The sequencing was carried out by Illumina HiSeq 2000 platform (Illumina ...
-
bioRxiv - Genomics 2022Quote: ... Metagenomic libraries of three species were prepared with NEBNext Ultra II DNA Library Prep Kit (BioLabs, New England) with a target insert length of 350 bp and sequenced on Illumina NovaSeq 6000 S4 (2×150bp reads) ...
-
bioRxiv - Genomics 2022Quote: ... The BxbI attP site was then added using the Q5 Site-Directed Mutagenesis Kit (Q5 SDM, NEB #E0554). To add a library of diverse random barcodes ...
-
bioRxiv - Genomics 2022Quote: ... DNA libraries were prepared from validated ChIP material using NEBNext Ultra II DNA library kit (New England Biolabs) protocol without size selection ...
-
bioRxiv - Genomics 2023Quote: Genomic DNA from a RCC bull was extracted using the Monarch Genomic DNA Purification Kit (New England Biolabs) according to the manufacturer’s guidelines ...
-
bioRxiv - Cell Biology 2023Quote: ... RNAseq libraries were prepared for Illumina using the NEBNext Ultra-Directional RNA library preparation kit (New England Biolabs). Library quality was confirmed using an Agilent BioAnalyzer 2100 and quantified using the NEBNext Library Quant Kit for Illumina (New England Biolabs) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... DNA libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit by New England Biolabs (NEB) and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
-
bioRxiv - Genetics 2022Quote: ... The sequencing library was prepared according to the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Specifically ...
-
bioRxiv - Genetics 2023Quote: ... RNA was converted to cDNA using a ProtoScript® II First Strand cDNA Synthesis Kit (NEB Cat # E6560L). RT-qPCR was performed using a QuantStudio 3 96-well 0.2 ml block instrument and PowerUp SYBR Green Master Mix (ThermoFisher Cat # A25741) ...
-
bioRxiv - Immunology 2023Quote: ... The pEGIP-derived packaging vector pEGIP-hACE2 was generated by homologous recombination using the NEBuilder kit (NEB, E5520). The vector backbone was amplified using oligonucleotides with appropriate hACE2 sequence overhangs ...
-
bioRxiv - Molecular Biology 2023Quote: ... CUT&RUN libraries were made using the NEB Ultra II DNA Library Prep Kit for Illumina (NEB #E7645L), and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
-
bioRxiv - Genomics 2023Quote: Libraries were built on beads using the NEBNext Ul-tra II DNA Library Prep kit (NEB, Cat # E7645L). Biotin pull down was initiated py pipetting 20 µL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Concentrations of libraries were measured with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2023Quote: ... Library preparation was completed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced using MiSeq v3 Paired End 150 bp (Illumina) ...
-
bioRxiv - Immunology 2023Quote: Site-directed mutagenesis of Unc93b1 was performed with the Q5 Site Directed Mutagenesis Kit (E0552S, New England Biolabs) according to the manufacturer’s instructions.
-
bioRxiv - Genomics 2023Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Preparation Kit for Illumina (New England BioLabs), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... cahirinus animal using a Monarch HMW DNA Extraction Kit for Cells & Blood (T3050, New England Biolabs, Ipswich MA) following the manufacturer’s recommended protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... IP protocol described in (11) was followed thereafter and Epimark® N6-Methyladenosine Enrichment Kit (New England Biolabs) was used ...
-
bioRxiv - Bioengineering 2023Quote: ... GLuc was inserted into the digested backbone through Gibson Assembly Hi-Fi kit (New England Biolabs, Ipswich, MA). AAV-hSyn-hM3Dq-RAM-d2tTA was constructed as previously described19 ...
-
bioRxiv - Cell Biology 2023Quote: ... 1ng pcDNA3.3-OCT4 was added for the amplification using the Q5 High-Fidelity DNA Polymerase kit (NEB, M0491). pcDNA3-EGFP (addgene ...
-
bioRxiv - Genomics 2023Quote: Purified cDNA was used as an input for the Nextera XT DNA library preparation kit (New England Biolabs). Library quality and size was determined using a TapeStation (Agilent Technologies ...
-
bioRxiv - Developmental Biology 2023Quote: ... A library was prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... 50 µg of lysostaphin and 100 µl of the tissue lysis buffer (Monarch Genomic DNA purification kit, NEB). Cells were lysed at 37°C for 30 min and then processed following the manufactures instructions.
-
bioRxiv - Plant Biology 2023Quote: ... Amplified PCR products with appropriate expected sizes were purified with the Monarch® DNA Gel Extraction Kit (NEB). Purified PCR products were cloned into the pMiniT 2.0 vector (NEB ...
-
bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were constructed using the NEBNext Ultra RNA Library Preparation Kit for Illumina (NEB, USA, Catalog #: E7530L) in accordance with the manufacturer’s guidelines ...
-
bioRxiv - Plant Biology 2024Quote: ... before library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequencing on the NovaSeq6000 (Illumina).
-
bioRxiv - Cell Biology 2024Quote: ... In vitro transcription was carried out with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), followed by the template DNA digestion with DNase I (Takara Bio) ...
-
bioRxiv - Cell Biology 2024Quote: ... Library fragment size and concentrations were quality verified by qPCR using the NEBNext Library Quant Kit (NEB, E7630) and loading 1:1 ...
-
bioRxiv - Immunology 2024Quote: ... cDNA libraries were prepared using NEBNext® Ultra DNA Library Prep for Illumina® kit (NEB, catalog: E7370) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... The hsp-4 dsRNA was synthesized in vitro with a HiScribe™ T7 RNA Synthesis Kit (NEB, E2050). Subsequently ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and amplified PCR fragments were purified by column purification using the Monarch PCR Cleanup Kit (New England Biolabs) following the manufacturer’s protocols.
-
bioRxiv - Cell Biology 2024Quote: ... Reverse transcription (RT) of RNA was performed using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs), in accordance with the manufacturer’s manual ...
-
bioRxiv - Microbiology 2024Quote: ... The resulting RNA was purified and concentrated with a Monarch RNA Clean-up Kit (NEB, Ipswich, MA, USA), quantified ...
-
bioRxiv - Immunology 2024Quote: ... and was subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). After ExoSAP-IT (Affymetrix ...
-
bioRxiv - Microbiology 2024Quote: ... rRNA depletion was carried out from 100ng of total RNA with NEBNext bacterial rRNA depletion kit (NEB#e7850). NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
-
bioRxiv - Genomics 2024Quote: ... 200 ng of sheared DNA was processed using the NEBNext Enzymatic Methyl-seq Kit (E7120; NEB, Ipswich, MA) following the manufacturer’s instructions for large insert libraries ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Duplexed oligonucleotides were ligated into dephosphorylated and BsmBI-digested pLB-Cas9 using the Quick Ligase kit (NEB M2200S) to generate transfer plasmid with RIPK1 guide sequence ...
-
bioRxiv - Immunology 2024Quote: ... and poly-A enriched before library preparation using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, USA). For each library ...
-
bioRxiv - Microbiology 2024Quote: ... RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) and sequenced using a NextSeq 550 75 Cycle High Output kit for single-end sequencing.
-
bioRxiv - Genetics 2024Quote: ... RNA was converted to cDNA using a ProtoScript® II First Strand cDNA Synthesis Kit (NEB Cat # E6560L). RT-qPCR was performed using a QuantStudio 3 96-well 0.2 ml block instrument and PowerUp SYBR Green Master Mix (ThermoFisher Cat # A25741) ...