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Citations for New England Biolabs :
5501 - 5550 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Extracted RNA was converted to cDNA with a ProtoScript II First strand cDNA synthesis kit (New England Biolabs) before use as a template for PCR ...
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bioRxiv - Systems Biology 2024Quote: The GFP-NPM1 cDNA was obtained from Addgene (#17578) and the NPM1c mutation was introduced and edited using Q5 Site-Directed Mutagenesis Kit (New England Biolabs). U2OS cells were transiently transfected with GPF-NPM1 or GFP-NPM1c overnight as described above ...
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bioRxiv - Immunology 2024Quote: ... Extracted RNA was amplified using Luna® Universal Probe One-Step RT-qPCR Kit (NEB, Ipswich, MA, E3006S) according to the manufacturer’s directions on a QuantStudio3 (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: The puromycin-conjugated mRNA templates were translated using a PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) with the addition of PURExpress Disulfide Bond Enhancer (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... A-tailed and ligated with barcoded adapters compatible with Illumina sequencing using a Quick Ligase Kit (NEB, M2200L). Each of the eight barcoded DNA libraries was pooled ...
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bioRxiv - Synthetic Biology 2024Quote: ... while amplicons from plasmids were purified using the Monarch Gel DNA Extraction Kit (New England Biolabs, Cat# T1020L) to eliminate any remaining background ...
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bioRxiv - Microbiology 2024Quote: ... 200 µL of RNA was then used for cDNA synthesis using LunaScript RT SuperMix Kit (New England Biolabs). Resulting cDNA was next used as input for a quantitative (q)PCR reaction using Luna Universal qPCR Master Mix (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... The transcribed reporter mRNA was isolated using the Monarch RNA Cleanup Kit (New England Biolabs, Cat. No. T2040L), eluted with nuclease-free water and quantified as mentioned above.
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bioRxiv - Immunology 2024Quote: ... CUT&RUN libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocols with modifications as described below ...
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bioRxiv - Molecular Biology 2024Quote: ... End-Repair and A-tailing was performed with the NEBNext Ultra II DNA library Prep Kit (NEB, E7645) for 30min at 20°C with constant mixing ...
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bioRxiv - Microbiology 2024Quote: ... Polymorphism substitutions were generated by site-directed mutagenesis using the Q5-site directed mutagenesis kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted from each samples using the Monarch total RNA miniprep kit (NEB product number T2010S) TRIzol extraction protocol with on-column DNase treatment ...
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bioRxiv - Microbiology 2024Quote: ... and assembled with pET-22b(+) DNA using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520S) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). These libraries were subsequently sequenced for paired-end reads of 2×100 bp on an MGI system at a sequencing depth of 50X.
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bioRxiv - Molecular Biology 2024Quote: ... followed by cDNA library generation using NEBNext Ultr II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to the manufacturer’s protocol and deep sequencing on Illumina HiSeq 2500 platform.
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bioRxiv - Genomics 2024Quote: ... Psoralen pull-down DNA was further purified using a Monarch PCR & DNA clean-up kit (New England Biolabs), eluted in 15 μl of EB buffer (10 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... Loss-of-function mutations were generated using site directed mutagenesis using Q5 Site-directed mutagenesis kit (E0552S, NEB) with the HA::SQSTM1/p62 plasmid as template (Addgene#280227) ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was extracted from each pool using the Monarch® Total RNA Miniprep Kit (New England Biolabs) including on-column DNase treatment ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized on 2 µg of total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... cDNAs were transcribed in vitro to cRNAs using the HiScribe® T7 Arca mRNA Kit (New England BioLabs). The Cx43 antisense oligonucleotide (Sequence ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were prepared from 100 ng input gDNA as described in (10.1371/journal.pgen.1010452) using the NEBNext Ultra II DNA library preparation kit (New England Biolabs), in combination with the EZ-96 DNA Methylation-Gold MagPrep kit (Zymo Research ...
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bioRxiv - Plant Biology 2024Quote: MeRIP-seq was conducted using a combination of methods from previously study36 and a commercial kit (NEB, E1610S). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... followed by first-strand cDNA synthesis with the Next Ultra II RNA Library Prep Kit for Illumina (NEB) and Next Multiplex Oligo for Illumina (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... Utilizing the NEBNext® UltraTM II RNA Library Prep Kit for Illumina® (New England Biolabs, Massachusetts, USA), RNA-Seq libraries were prepared and sequenced on Illumina’s advanced NovaSeq 6000 V1.5 platform (Illumina Inc. ...
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bioRxiv - Plant Biology 2024Quote: ... ChIP sequencing libraries were constructed using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs Inc, E7103) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... sgRNA was either in vitro transcribed (IVT-sgRNA) using EnGen® sgRNA Synthesis Kit (New England Biolabs, USA) or chemical synthesized and modified (CSM-sgRNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and subsequently converted into sequencing libraries using the NEBNext® Ultra™ II DNA Library Prep Kit (NEB).
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bioRxiv - Molecular Biology 2024Quote: ... 8 ng of eluted DNA was used to build the library with the NEBNext Ultra II kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA-seq library was prepared using NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB), and deep sequencing was conducted using the PE150 strategy on NovaSeq 6000 platform.
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using The NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, E7420L). Library quality was assessed using Agilent 2100 bioanalyzer (High Sensitivity DNA Kit) ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted after 48 hours of siRNA knockdown using the Monarch Total RNA Miniprep Kit (T2010S, NEB). RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (4368814 ...
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bioRxiv - Genomics 2024Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs E7645) and sequenced paired end on a NovaSeq with an S1 single lane 100 cycle kit.
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bioRxiv - Molecular Biology 2024Quote: ... site- directed mutagenesis was performed using the Q5 Site-Directed Mutagenesis Kit (NEB, Ipswich, MA, USA, Cat. #E0554S). The plasmids pLenti-CRISPRa and pLenti-CRISPRv2-puro were obtained from Addgene (Watertown ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were generated from ChIP-DNA using the NEBNext Ultra Library Prep Kit for Illumina (NEB #E7645) and NEBNext Multiplex Oligos for Illumina dual-index primers (one of #E6440 ...
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bioRxiv - Microbiology 2024Quote: ... DNASeq library compatible with short read Illumina sequencing was generated using the NEB Ultra DNA library Kit (NEB) starting with 500 ng of DNA ...
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bioRxiv - Microbiology 2024Quote: ... and CarD-gFLAG (LK2899) were designed using NEBuilder Assembly Tool (https://nebuilder.neb.com/) and constructed using Gibson assembly kit (NEB) as following ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial rRNA depletion was performed using the NEBNext rRNA Depletion Kit (Bacteria) (New England Biolabs, Ipswich, MA,USA). Plant rRNA depletion was performed using TruSeq Stranded Total RNA with Ribo-Zero Plant (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (#7530 L, NEB, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using the NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) (New England Biolabs) following the manufacturer’s protocol (including RNase H and DNase I digestions) ...
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bioRxiv - Neuroscience 2024Quote: ... Missense variants were generated in MECP2-pENTRY either using the Q5 mutagenesis kit (New England Biolabs, USA, # E0554) in house ...
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bioRxiv - Genomics 2024Quote: ... we used the NEBNext® Ultra™ II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA) to prepare sequencing libraries following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... the vector was then modified using rounds of site-directed mutagenesis (Q5 Site-directed mutagenesis kit #E0554S, NEB) to first introduce an M56L mutation in Tn5 (Primers:Tn5M56L_F ...
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bioRxiv - Synthetic Biology 2024Quote: ... Non-fluorescent RNA was transcribed using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, cat. E2040S). Following in vitro transcription by either kit ...
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bioRxiv - Synthetic Biology 2024Quote: DNA libraries targeting specific residue positions were constructed using the Q5 Site-Directed Mutagenesis Kit (Cat#E0552S, NEB) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and transformed cells with the resulting library after column-based purification with a commercial electroporation kit (NEB #T1020L). We grew populations of E ...
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bioRxiv - Bioengineering 2024Quote: Purified RNA samples were prepared with an NEB Ultra II RNA kit (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The in vitro transcribed RNA was purified using Monarch RNA Clean-up Kits (NEB T2030; Ipswich, MA, USA). Subsequently ...
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bioRxiv - Genetics 2024Quote: ... pET28b-hPNPM745T were constructed by site directed mutagenesis using Q5® Site-Directed Mutagenesis Kit (New England Biolabs) with ad hoc primers ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and then libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA).
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bioRxiv - Genomics 2024Quote: ... ChIP-seq sequencing libraries were generated using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, #E7645L) according to manufacturer’s protocol ...