Labshake search
Citations for New England Biolabs :
5451 - 5500 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... a pair of single guide RNAs (sgRNAs) were synthesized using the EnGen sgRNA Synthesis Kit (E3322V) from NEB; a total of 1μg of the sgRNA pair along with 20 pmol recombinant Cas9 (EnGen S ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). These libraries were subsequently sequenced for paired-end reads of 2×100 bp on an MGI system at a sequencing depth of 50X.
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bioRxiv - Molecular Biology 2024Quote: ... followed by cDNA library generation using NEBNext Ultr II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to the manufacturer’s protocol and deep sequencing on Illumina HiSeq 2500 platform.
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was extracted from each pool using the Monarch® Total RNA Miniprep Kit (New England Biolabs) including on-column DNase treatment ...
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bioRxiv - Microbiology 2024Quote: ... 500 ng of total RNA underwent ribosomal depletion with the NEBNext rRNA Depletion Kit (Bacteria) (NEB catalog # E7850) and library preparation with the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202 ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized on 2 µg of total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... cDNAs were transcribed in vitro to cRNAs using the HiScribe® T7 Arca mRNA Kit (New England BioLabs). The Cx43 antisense oligonucleotide (Sequence ...
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bioRxiv - Cell Biology 2024Quote: ... sgRNA was either in vitro transcribed (IVT-sgRNA) using EnGen® sgRNA Synthesis Kit (New England Biolabs, USA) or chemical synthesized and modified (CSM-sgRNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and subsequently converted into sequencing libraries using the NEBNext® Ultra™ II DNA Library Prep Kit (NEB).
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bioRxiv - Molecular Biology 2024Quote: ... 8 ng of eluted DNA was used to build the library with the NEBNext Ultra II kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA-seq library was prepared using NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB), and deep sequencing was conducted using the PE150 strategy on NovaSeq 6000 platform.
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using The NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, E7420L). Library quality was assessed using Agilent 2100 bioanalyzer (High Sensitivity DNA Kit) ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted after 48 hours of siRNA knockdown using the Monarch Total RNA Miniprep Kit (T2010S, NEB). RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (4368814 ...
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bioRxiv - Microbiology 2024Quote: ... aeruginosa PAO1 genomic DNA (gDNA) and cleaned up using the Monarch PCR & DNA Cleanup kit (New England Biolabs) were used for Gibson assembly together with pEX18Gm cut with EcoRI-HF and Xbal ...
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bioRxiv - Neuroscience 2024Quote: ... The cleaved chromatins were collected from the supernatant and purified using Monarch PCR&DNA Cleanup Kit (T1030L, BioLabs) according to the manufacture’s instruction.
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bioRxiv - Developmental Biology 2024Quote: ... Sequencing libraries were prepared with NEBNext Ultra II DNA library preparation kit for Illumina (New England Biolabs, E7645S).
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bioRxiv - Microbiology 2024Quote: ... and CarD-gFLAG (LK2899) were designed using NEBuilder Assembly Tool (https://nebuilder.neb.com/) and constructed using Gibson assembly kit (NEB) as following ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial rRNA depletion was performed using the NEBNext rRNA Depletion Kit (Bacteria) (New England Biolabs, Ipswich, MA,USA). Plant rRNA depletion was performed using TruSeq Stranded Total RNA with Ribo-Zero Plant (Illumina ...
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bioRxiv - Genetics 2024Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB) following manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... Media was directly used to detect viral RNA using Luna® Universal One-Step RT-qPCR Kit (NEB) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were generated from ChIP-DNA using the NEBNext Ultra Library Prep Kit for Illumina (NEB #E7645) and NEBNext Multiplex Oligos for Illumina dual-index primers (one of #E6440 ...
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bioRxiv - Microbiology 2024Quote: ... DNASeq library compatible with short read Illumina sequencing was generated using the NEB Ultra DNA library Kit (NEB) starting with 500 ng of DNA ...
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bioRxiv - Immunology 2024Quote: ... sequencing libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) and sequenced on an Illumina platform ...
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bioRxiv - Microbiology 2024Quote: Total RNA was reverse transcribed into cDNA using the LunaScript® RT SuperMix Kit (New England Biolabs, E3010). The reaction mixture was prepared in a total volume of 20 µl ...
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bioRxiv - Microbiology 2024Quote: Enriched RNA underwent library preparation using the NEBNext multiplex small RNA library prep kit (NEB product number E7300S) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... NEBNext Ultra II Directional Library Prep Kit and NEBNext® Multiplex Oligos for Illumina® (NEB, Lynn, MA). Each library was subject to sequencing (Illumina NovoSeq X ...
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bioRxiv - Neuroscience 2024Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Immunology 2024Quote: The puromycin-conjugated mRNA templates were translated using a PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) with the addition of PURExpress Disulfide Bond Enhancer (New England Biolabs ...
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bioRxiv - Immunology 2024Quote: ... Extracted RNA was amplified using Luna® Universal Probe One-Step RT-qPCR Kit (NEB, Ipswich, MA, E3006S) according to the manufacturer’s directions on a QuantStudio3 (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... CUT&RUN libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocols with modifications as described below ...
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bioRxiv - Molecular Biology 2024Quote: ... site- directed mutagenesis was performed using the Q5 Site-Directed Mutagenesis Kit (NEB, Ipswich, MA, USA, Cat. #E0554S). The plasmids pLenti-CRISPRa and pLenti-CRISPRv2-puro were obtained from Addgene (Watertown ...
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bioRxiv - Microbiology 2024Quote: ... Assembly of the corresponding plasmids was conducted using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) according to the instruction manual ...
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bioRxiv - Synthetic Biology 2024Quote: ... while amplicons from plasmids were purified using the Monarch Gel DNA Extraction Kit (New England Biolabs, Cat# T1020L) to eliminate any remaining background ...
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bioRxiv - Microbiology 2024Quote: ... 200 µL of RNA was then used for cDNA synthesis using LunaScript RT SuperMix Kit (New England Biolabs). Resulting cDNA was next used as input for a quantitative (q)PCR reaction using Luna Universal qPCR Master Mix (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... Extracted RNA was converted to cDNA with a ProtoScript II First strand cDNA synthesis kit (New England Biolabs) before use as a template for PCR ...
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bioRxiv - Systems Biology 2024Quote: The GFP-NPM1 cDNA was obtained from Addgene (#17578) and the NPM1c mutation was introduced and edited using Q5 Site-Directed Mutagenesis Kit (New England Biolabs). U2OS cells were transiently transfected with GPF-NPM1 or GFP-NPM1c overnight as described above ...
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bioRxiv - Genomics 2024Quote: ... we used the NEBNext® Ultra™ II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA) to prepare sequencing libraries following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Polymorphism substitutions were generated by site-directed mutagenesis using the Q5-site directed mutagenesis kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted from each samples using the Monarch total RNA miniprep kit (NEB product number T2010S) TRIzol extraction protocol with on-column DNase treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... The transcribed reporter mRNA was isolated using the Monarch RNA Cleanup Kit (New England Biolabs, Cat. No. T2040L), eluted with nuclease-free water and quantified as mentioned above.
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bioRxiv - Molecular Biology 2024Quote: ... End-Repair and A-tailing was performed with the NEBNext Ultra II DNA library Prep Kit (NEB, E7645) for 30min at 20°C with constant mixing ...
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bioRxiv - Microbiology 2024Quote: ... and assembled with pET-22b(+) DNA using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520S) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB; E7770L). Pooled libraries were sequenced on the NovaSeq 6000 instrument using paired-end 51 cycle reads.
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bioRxiv - Cancer Biology 2024Quote: ... The library was constructed using the NEB Next®Ultra™RNA Library Preparation Kit (New England Biolabs). The library quality was assessed using the Agilent Bioanalyzer 2100 system and then sequenced using the Illumina Novaseq platform ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for the PCR were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for the PCR were as follows ...
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bioRxiv - Plant Biology 2024Quote: ... sequencing libraries were fabricated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA). The resulting libraries underwent sequencing on an Illumina Hiseq 4000 platform ...
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bioRxiv - Microbiology 2024Quote: ... Barcoded libraries were made with NEBNext Ultra II directional RNA library prep kit for Illumina (New England Biolabs). Libraries were pooled and sequenced (single-end 75-bp reads ...
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bioRxiv - Plant Biology 2024Quote: ... we used NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, catalog no. E7760L) protocol ...