Labshake search
Citations for New England Biolabs :
5251 - 5300 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2024Quote: ... PCR products were purified with a Monarch PCR & DNA clean-up kit (New England Biolabs, Whitby, ON, Canada) and used as template to reamplify the products to incorporate a Kozak translation initiation sequence prior to the start codon (Kozak ...
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bioRxiv - Molecular Biology 2023Quote: ... Removal of rRNA was carried out by use of the NEBNext rRNA Depletion Kit Human/Mouse/Rat (NEB) followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina ...
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bioRxiv - Physiology 2024Quote: RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to manufacturer’s polyA mRNA workflow at the Advanced Analysis Center at the University of Guelph (Guelph ...
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bioRxiv - Neuroscience 2024Quote: ... CUT&RUN libraries were made using the NEB Ultra II DNA Library Prep Kit for Illumina (NEB E7645L), and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Microbiology 2024Quote: ... non incorporated primers were removed using the Monarch PCR and DNA clean up kit (New England Biolabs, T1030), using a binding buffer ratio to DNA of 3:1 ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product of the selected deletion mutant was purified using Monarch PCR and DNA cleanup kit (NEB) and sent for sequencing at Eurofins Genomics LLC (Louisville ...
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bioRxiv - Molecular Biology 2024Quote: ... and further cleaning (to get rid of DNA contamination) with Monarch® Total RNA Miniprep Kit (NEB, T2010S) by following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Ribosomal RNA was removed with NEB Ribo-Zero Plus rRNA Depletion Kit (New England Biolabs, Cat No. 20037135) prior to RNA-seq library preparation ...
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bioRxiv - Cancer Biology 2024Quote: ... Total cDNA libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB #E7765).
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bioRxiv - Genetics 2024Quote: ... RNA-Seq libraries were prepared with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Capillary electrophoresis (Agilent Bioanalyser and Agilent Tapestation ...
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bioRxiv - Genomics 2024Quote: ... The linearized vector was assembled with the amplified oligo pool using the NEBuilder HiFi DNA assembly kit (NEB) by mixing 50 ng of the linearized pIS-0 vector and 2 µL of the amplified oligonucleotide pool in a 20 µL reaction volume (Figure S2A) ...
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bioRxiv - Molecular Biology 2024Quote: ... the products were used to generate RNA transcripts using the HiScribe T7 In Vitro Transcription Kit (NEB, E2040S) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was then directly used in the Luna® Universal One-Step RT-qPCR Kit (New England BioLabs). Primers listed in supplemental table 10.
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bioRxiv - Microbiology 2024Quote: ... The two PCR products were extracted and assembled using the NEBuilder HiFi DNA Assembly kit (New England Biolabs). The assembly product was transformed into E ...
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bioRxiv - Immunology 2024Quote: ... Ligations into the respective backbone were performed overnight at 16°C using T4 DNA Ligase Kit (NEB, #M0202L). Ligations were transformed into NEB Stable E ...
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bioRxiv - Evolutionary Biology 2024Quote: Libraries were prepared using the NEBNext Ultra II FS Library Prep Kit (New England Biolabs, Ipswich, MA, USA), following the manufacturer’s instructions with inserts of approximately 350 bp ...
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bioRxiv - Genomics 2024Quote: Hadrurus arizonensis DNA was extracted using the MonarchⓇHigh Molecular Weight (HMW) DNA Extraction Kit: Tissue Protocol (NEB, T3060). Changes made to the protocol include ...
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bioRxiv - Microbiology 2024Quote: Cell-free transcription and translation reactions were carried out using PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufactureŕs protocol ...
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bioRxiv - Microbiology 2024Quote: ... Twenty microlitre reaction mixtures were prepared using the Luna® Universal qPCR Master Mix kit (New England Biolabs) as follows ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was either extracted with phenol-chloroform followed by Monarch Total RNA Miniprep kit (New England Biolabs) or with the Quick-RNA Miniprep kit (Zymo) ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed and mRNA was isolated and purified using the Magnetic mRNA Isolation Kit (New England BioLabs). Resulting purified mRNA was quantified using Nanodrop.
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bioRxiv - Immunology 2024Quote: ... Hoil1 and Hoip mutations and deletions were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). FLAG-tagged cDNAs were transferred into pTrip-CMV-Puro-T2A-RFP (72 ...
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bioRxiv - Neuroscience 2024Quote: One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England Biolabs) in a total volume of 10 µl and a template concentration of 50 ng/µl according to manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Sequencing of the library was performed using Illumina HiSeq2500 and NextSeq2000 to produce 65-bp single-end reads and 36-bp paired-end reads ...
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bioRxiv - Cancer Biology 2024Quote: ... and barcoded libraries were made using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries were pooled and single-end sequenced (1×75 bp ...
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bioRxiv - Microbiology 2024Quote: ... mApple was cloned in place of the eYFP gene using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and BstXI and NotI restriction sites as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: T7 constructs were ordered from IDT and synthesized using HiScribe T7 High Yield RNA Synthesis Kit (NEB, #E2040S). RNA was purified via RNA extraction ...
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bioRxiv - Cell Biology 2024Quote: ... and then constructed the library using NEBNext® Ultra™ II for DNA kit (NEB; P/N E7645L). We used the Agilent Bioanalyzer to assess the quality of the library and a qPCR approach with the KAPA Library Quantification Kit (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were prepared using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... assembled with GST tag from pGEX6P plasmid and inserted into pACEBac1 vector via HIFI DNA assembly kit (NEB). The full length KIF5B in pACEBac1 was replaced with a C-terminal TEV-Twin-Strep-tag via HIFI DNA assembly kit (NEB) ...
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bioRxiv - Genomics 2024Quote: ... 3.) Library preparation was performed using the NEBNext Ultra II library preparation kit for Illumina (New England Biolabs) and 4. ...
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bioRxiv - Developmental Biology 2024Quote: ... Template pDNA was linearized and ARCA-capped IVT-mRNA was produced using the T7 HiScribe kit from NEB, pseudo-uridine was incorporated into the reaction ...
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bioRxiv - Physiology 2023Quote: ... The cDNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Extracted RNA was treated with DNaseI and purified using Monarch RNA Cleanup Kit (New England Biolabs, Massachusetts, USA). The cDNA was synthesised from approximately 1000 ng of RNA using ReverTra Ace qPCR RT Master Mix with gDNA remover (TOYOBO ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was prepared from 1µg of RNA using the LunaScript® RT SuperMix Kit (New England Biolabs, E3010L). A no template control (NTC ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were constructed with the NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, #E7645S) and sequenced on an Illumina HiSeq 3000 platform to generate a minimum of 20 million 150-bp reads per sample.
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bioRxiv - Genetics 2024Quote: ... Plasmids were then isolated using the Qiagen Miniprep kit and genotyped by digesting with BsrGI (New England Biolabs) to detect the presence of the TARS1 insert ...
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bioRxiv - Genomics 2024Quote: ... RNA (100 ng) was used for cDNA synthesis using the ProtoScript II First Strand cDNA Synthesis Kit (NEB) with random hexamers ...
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bioRxiv - Immunology 2024Quote: cDNAs were synthesized from isolated RNA samples using ProtoScript II First Strand cDNA Synthesis Kits (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and in vitro transcription and polyadenylation was performed using HiScribe T7 ARCA mRNA Kit (with tailing) from NEB, following recommended procedure ...
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bioRxiv - Immunology 2024Quote: ... IVT DI was in vitro transcribed using a HiScribe T7 High Yield RNA synthesis kit (New England Biolabs) followed by DNAse I clean-up with a TURBO DNA-free kit (Thermo-Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and were sequenced on an Illumina NovaSeq 150bp paired-end platform ...
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bioRxiv - Genetics 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional Library Prep Kit for Illumina (E7760, NEB). The size of the libraries was determined using a Bioanalyzer ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries for RNA-seq were prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and sequenced on the NextSeq 500 platform (Illumina ...
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bioRxiv - Genomics 2023Quote: ... Commercial and breeding program samples were extracted from frozen tissue using the Monarch Genomic DNA Purification Kit (NEB), as per manufacturer’ s instructions using the enzymatic cleanup protocol with both proteinase K and RNase A ...
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bioRxiv - Biochemistry 2024Quote: ... Mutants of PARIS were constructed from pFR85 using the Gibson method38 or Q5 site-directed mutagenesis kit (NEB). The PARIS triggers Ptr1 (T5 ORF094 ...
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bioRxiv - Biochemistry 2023Quote: ... DNA synthesis was visualized by detecting biotin with the Phototope-Star detection kit (New England Biolabs Ref N7020S) and images acquired on a Chemidoc system (Biorad).
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bioRxiv - Synthetic Biology 2023Quote: ... Sequencing libraries were generated using the NEBNext ® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: Ifnb1 mRNA 3’ UTR (NM_002176.4) was synthesized using the HiScribe T7 High Yield RNA synthesis kit (NEB, E2040S) through in vitro transcription ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was extracted from flash-frozen samples with the Monarch® Total RNA Miniprep Kit (New England Biolabs) including the DNase treatment step ...