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Citations for New England Biolabs :
5151 - 5200 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Pre-adenylated DNA linker(DNA oligo purchase from IDT was adenylated using 5′ DNA Adenylation Kit (NEB,E2610S)) was ligated to the 3’-end of RNA in a 20 µl reaction (25% PEG8000 ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA libraries were made using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs # E7645S) according to the manufacturer’s recommendations.
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bioRxiv - Bioengineering 2020Quote: ... The RT-PCR detection was conducted by using the Luna Universal One-Step RT-qPCR kit from NEB and following its protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... This fragment was inserted in front of a codon optimized GFP via the HiFi DNA Assembly kit (NEB) with the addition of a membrane-tethering CAAX domain at the C-terminus to visualize the morphology of the cells expressing the reporter protein ...
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bioRxiv - Microbiology 2021Quote: ... Point mutations were introduced by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England BioLabs, Inc., USA) using primer pairs P11/P12 (G63S) ...
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bioRxiv - Biophysics 2021Quote: ... The Vimentin-HALO construct was obtained after cloning using the HiFi DNA Assembly Cloning Kit (Biolabs cat # E5520S) starting from the tubulin-HALO plasmid (addgene #64691 ...
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bioRxiv - Biochemistry 2021Quote: ... also C-terminal) FT MYOC variants were generated by DNA assembly (NEBuilder HiFi DNA Assembly Cloning Kit, NEB) by combining a PCR-amplified eGLuc2 product from a pcDNA3 MYOC eGLuc2 plasmid (originally described previously8) ...
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bioRxiv - Biochemistry 2021Quote: ... metoecus Cmr interference complex (VmeCmr) was assembled using the NEBuilder® HiFi DNA Assembly kit (New England Biolabs) following the Gibson assembly strategy (43) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 μg of DNA library was used as a template for mRNA synthesis by HiScribe T7 kit (NEB). The product was purified by NH4Ac precipitation and dissolved in RNAse-free water to a final concentration of 3 μg/ul.
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bioRxiv - Genomics 2020Quote: ... Protocols and reagents from NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs) were used for end repair ...
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bioRxiv - Bioengineering 2020Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Neuroscience 2020Quote: ... Truncated Hsp70 constructs were made using a Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was depleted of ribosomal RNAs using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) ...
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bioRxiv - Genomics 2021Quote: ... Libraries were then constructed with the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB) following the manufacturer’s instructions and sequenced on an Illumina paired-end platform with 150 bp per read length by Annoroad Gene Technology Co. ...
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bioRxiv - Developmental Biology 2021Quote: ... ChIP-seq libraries were prepared using the NEB Next Ultra II DNA Library Prep kit (New England Biolabs) following the manufacturer’s instruction and subjected to high-throughput sequencing on an Illumina HiSeq 2500 system (150 cycles ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently in-vitro transcribed using HiScribe™ T7 Quick High Yield RNA Synthesis Kit from NEB (#E2050S). The IRE-1 RNase-specific inhibitor 4μ8c was used at 5 μM.
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bioRxiv - Bioengineering 2021Quote: ... sgRNA was transcribed in vitro using the HiScribe Quick T7 High Yield RNA Synthesis Kit (New England Biolabs). The transcribed sgRNA was treated with DNaseI (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Libraries were prepared for RNA-sequencing using NEBNextUltra Directional RNA Library Prep Kit for Illumina (New England Biolabs), according to manufacturer’s protocol and single end sequencing for 50 cycles were done using Illumina HiSeq 2500 platform.
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bioRxiv - Cell Biology 2021Quote: ... RNA sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB England BioLabs). Fragmented and randomly primed 2 × 150□bp paired-end libraries were sequenced using Illumina HiSeq X10.
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bioRxiv - Biophysics 2020Quote: ... Y737W and R805W substitution in were introduced into UCS-wt using Q5® Site-Directed Mutagenesis Kit (NEB). Recombinant protein expression was induced in BL21 DE3 when optical density (OD600 ...
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bioRxiv - Genomics 2020Quote: ... A construct containing the alternate allele was then created using the NEB Q5 SDM kit (New England Biolabs). The primer sequences used were as follows:
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bioRxiv - Microbiology 2020Quote: ... Libraries were prepared with NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs). Paired-end (150-bp ...
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bioRxiv - Immunology 2021Quote: ... Capped SAM was synthesized in vitro using HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs) and purified using a RNeasy Maxi Kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragment was cloned into AscI-digested pBYL2 vector via HiFi DNA Assembly Cloning kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries were constructed with the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB, E7760), amplified with custom oligonucleotides ...
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bioRxiv - Molecular Biology 2020Quote: ... Ribosomal RNA was depleted from 200 μg of the RNA using the NEBNext rRNA Depletion Kit (NEB E6310X) and then a cDNA library was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB E7530L) ...
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). For strand-specific RNA-sequencing ...
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bioRxiv - Molecular Biology 2021Quote: sRNA-seq libraries were prepared using the NEB Next Small RNA-sequencing kit (NEB #E7300, New England BioLabs), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: sRNA-seq libraries were prepared using the NEB Next Small RNA-sequencing kit (NEB #E7300, New England BioLabs), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... libraries for sequencing were prepared with the NEB Next Ultra II RNA Prep Kit (New England BioLabs, Inc.) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... the myc tag was removed from the Ccz1-myc plasmid using NEB site-directed mutagenesis (SDM) kit (NEB) following manufacturer’s instructions and primers selected using NEBaseChanger tool (table 1) ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were generated using a NEBNext® Ultra RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... The RNA-Seq libraries were prepared with NEBNext® UltraTM RNA Library Prep Kit for Illumina (E7530L, NEB) by following manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... Sample and input libraries were prepared using NEBNext Ultra II DNA Library Prep kit (New England BioLabs E7645) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: RNA-Seq libraries were generated using the NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB #E7530). Libraries were quantified using the NEBNext Quant Kit (NEB #E7630) ...
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bioRxiv - Molecular Biology 2021Quote: ... Illumina libraries were prepared using NEBNext DNA Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturers protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Six variants were mutated on the basis of proPIF4-L:LUC using Q5 site-directed mutagenesis kit (NEB, E0552S) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... amino acid change-conferring substitutions were placed into the SHIV.C.CH505.3C backbone using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs #E0554S). Primers were designed according to the NEBaseChanger tool (https://nebasechanger.neb.com) ...
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bioRxiv - Neuroscience 2023Quote: RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770S) in conjunction with the NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... m6A-seq libraries were prepared using the NEBNext Ultra Directional RNA library preparation kit for Illumina (NEB #E7760). Library quality was checked by Agilent BioAnalyzer 2100 using the High Sensitivity DNA kit (Part number 5067-4626 ...
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bioRxiv - Molecular Biology 2023Quote: The SRRM1 entry clone from DNASU was modified using the Q5 site directed mutagenesis kit (New England Biolabs) to delete the predicted disordered region as annotated by Uniprot (https://www.uniprot.org/uniprotkb/Q8IYB3/entry) ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA of wild-type and stat3 mutants were generated by the ProtoScript First Strand cDNA Synthesis Kit (NEB) using random primers ...
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bioRxiv - Cancer Biology 2023Quote: ... ChIP-seq libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) and quality and concentration were assessed with the Bioanalyzer (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... for mRNA purification followed by NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: CDKN1A intron 1 APA 3’ splice site was deleted using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) as per the manufacturer’s instructions using forward primer (5’-TCCCCACCCCAAAATGACGCGCAGCC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... ONT Kit) was ligated to the RNA sample using 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA (∼10 ng) was used to make libraries using the NEBNext Ultra II kit (New England Biolabs) and sequenced on the NextSeq 2000 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: 9N_VRA3 adapter oligonucleotide (Supplementary Table 1) was pre-adenylated with the 5’ DNA Adenylation Kit (New England Biolabs) according to the manufacturer’s protocol at a 5X scale ...
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bioRxiv - Molecular Biology 2023Quote: ... was done using the Q5® Site-Directed Mutagenesis Kit from New England Biolabs (NEB; Ipswich, MA, USA), following their protocol with recommended annealing temperature and pETσ70 CFI ...