Labshake search
Citations for New England Biolabs :
5201 - 5250 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were generated using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, #E7645L) according to manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Genetic substitutions were introduced via site-directed mutagenesis using the Q5 site-directed mutagenesis kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: ... O-GlcNAc site were mutated into alanine with Q5® Site-Directed Mutagenesis Kit (NEB#E0554). Lentivirus were packed as previously described (Huang et al. ...
-
bioRxiv - Plant Biology 2023Quote: ... Sequencing libraries were generated using NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, USA) as reported previously (Jiang et al. ...
-
bioRxiv - Systems Biology 2023Quote: ... we pelleted each liquid culture by centrifugation and used the Monarch Plasmid Miniprep Kit (NEB #T1010L) to isolate plasmid according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... The libraries were indexed for multiplexing using NEBNext multiplex oligos kit for Illumina (New England Biolabs). A total of 54 libraries were generated ...
-
bioRxiv - Biochemistry 2022Quote: ... cDNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB) in accordance with the manufacturers’ instructions.
-
bioRxiv - Neuroscience 2023Quote: ... An HpaI site within W3SL was destroyed using the Q5® Site-Directed Mutagenesis Kit (NEB): pAAV-U6-TATAlox-CMV-EGFP-TATAlox-W3SL ...
-
bioRxiv - Neuroscience 2022Quote: ... Libraries were quantified with the NEBNext Library Quant Kit for Illumina according to manufacturer instructions (NEB). Pooled libraries were combined and sequenced with a 75 cycles NextSeq 500/550 High Output Kit on a NextSeq 500/550 machine (Illumina).
-
bioRxiv - Neuroscience 2022Quote: ... Sequencing libraries were generated using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2022Quote: ... We quantified the libraries using the NEBNext Library Quant Kit for Illumina (New England Biolabs, #E7630) based on the mean insert size provided by the Bioanalyzer ...
-
bioRxiv - Physiology 2022Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (New England BioLabs) following manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription was performed using a First-Strand cDNA Synthesis kit (New England Biolabs, Cat. #E6560) and random primers ...
-
bioRxiv - Molecular Biology 2022Quote: ... The synthesized cDNA was purified using Monarch® PCR and DNA Cleanup Kit (New England Biolabs). Poly-guanine was added at the cDNAs 3’ end using Terminal Transferase (Roche ...
-
bioRxiv - Molecular Biology 2022Quote: ... subsequently used to generate cDNA with LunaScript RT SuperMix Kit (New England Biolabs, catalog no. E3010S). Real-time PCR was performed using SYBR green gene expression assays (New England Biolabs ...
-
bioRxiv - Molecular Biology 2022Quote: ... and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs: E7760) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... libraries were constructed using NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB, E7770) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit (E7760, NEB). Quality control was checked through a Bioanalyzer ...
-
bioRxiv - Bioengineering 2022Quote: ... and library concentrations were measured with the NEBNext Library Quant Kit (New England Biolabs, Ipswich, MA). The libraries were pooled to a final concentration of 2 nM and run on a NextSeq sequencer (Illumina ...
-
bioRxiv - Molecular Biology 2022Quote: ... NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) was used for the construction of sequencing libraries ...
-
bioRxiv - Bioengineering 2022Quote: ... RNA was reverse-transcribed and PCR amplified using Luna Universal One-Step RT-PCR Kit (NEB). SARS-CoV-2 replication was assessed by using primers specific to the N mRNA (Forward 5’-CTCTTGTAGATCTGTTCTCTAAACGAAC-3’ ...
-
bioRxiv - Cell Biology 2022Quote: ... C55S and C62S mutants were generated using the Q5 site directed mutagenesis kit (New England Biolabs). The Cx36 pcDNA plasmid was PCR amplified with modified primers to introduce the corresponding deletions or substitutions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... IVT-produced RNA samples were purified using a Monarch® RNA Cleanup Kit (New England Biolabs). ssRNA during RNA extractions was degraded with RNase T1 (Thermo Scientific) ...
-
bioRxiv - Systems Biology 2023Quote: ... IVT was conducted using the HiScribe® T7 Quick High Yield RNA Synthesis Kit (NEB E2050S) - 1x T7 RNA Polymerase Mix ...
-
bioRxiv - Developmental Biology 2024Quote: ... and ligation to adapters with “NEBNext Ultra II DNA Library Prep Kit for Illumina” (NEB #E7645). Adapter-ligated libraries were completed by a PCR of 21 cycles of amplification ...
-
bioRxiv - Microbiology 2024Quote: ... and cDNA library preparation (NEBNext Ultra II Directional RNA Library Prep Kit - Bacteria (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... a sequencing library was prepared using NEBNext® Enzymatic Methyl-seq Kit (NEB; catalog number E7120S). Library sequencing was performed using NextSeq 2000 (Illumina ...
-
bioRxiv - Developmental Biology 2024Quote: ... RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, #E7420L), followed by sequencing on an Illumina Hiseq 4000 system ...
-
bioRxiv - Plant Biology 2024Quote: ... The sgRNA was first synthesised using the EnGen sgRNA synthesis kit New England Biolabs (NEB #E3322) before mixing with the Cas9-NLS to form the RNP complex ...
-
bioRxiv - Genetics 2024Quote: ... The resulting product was inserted into linearized pKR482 using NEBuilder HiFi DNA assembly kit (NEB E5520S) and transformed into 10-beta cells ...
-
bioRxiv - Synthetic Biology 2024Quote: ... library concentrations were quantified with qPCR using the NEBNext Library Quant Kit for Illumina (NEB, E7630S), and pre- and post-selection libraries were combined as two pools ...
-
bioRxiv - Molecular Biology 2023Quote: 7.5ug of total RNA was immunoprecipitated for m6A with EpiMark® N6-Methyladenosine Enrichment Kit (NEB) following the manufacture protocol with 10% of the sample RNA taken as input prior to the pulldown ...
-
bioRxiv - Microbiology 2023Quote: ... RT-qPCR was then performed using Luna Universal One-step RT-qPCR kit (New England Biolabs) following the provided protocol on a BioRad CFX Opus 96 quantitative Real-Time PCR machine ...
-
bioRxiv - Microbiology 2023Quote: ... Prepared libraries were quantified using a NEBNext Library Quant kit for Illumina E7630L (New England BioLabs) and pooled with volumes adjusted to normalize concentrations aiming for ∼700-fold genomic coverage for population samples or ∼200-fold genomic coverage for clones ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... cDNA was synthesized using the ProtoScript II Reverse Transcriptase kit (New England BioLabs, Évry-Courcouronnes, France). qRT-PCR was performed in quadruplets on a CFX384 Touch Real-Time PCR Detection System (Biorad ...
-
bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2024Quote: ... The SUGCT insert and pcDNA5/FRT vector were ligated by using the Quick Ligation Kit (NEB) and transformed to TOP10 chemically competent cells (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and then used as input for the NEBNext Ultra II RNA Library Prep Kit (NEB #E7770S). Sample preparation was performed as per manufacturer’s specifications ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... A genomic library was prepared using the NEBNext Ultra DNA Library Prep Kit (New England BioLabs) and sequenced on an Illumina NovaSeq (S4 Flow Cell ...
-
bioRxiv - Microbiology 2024Quote: ... and libraries for Illumina sequencing were performed using the NEBNext Ultra II RNA kit (NEB, #E7770L). Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes ...
-
bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA depletion was performed using the NEBNext rRNA Depletion Kit for Bacteria (New England Biolabs), spiked with a custom oligo pool ...
-
bioRxiv - Molecular Biology 2024Quote: ... while library concentration was determined using the NEBNext Library Quant Kit for Illumina (NEB, Cat# E7630). All input RNA had RNA integrity number (RIN ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
-
bioRxiv - Microbiology 2024Quote: ... 1 μg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse-transcriptase ...
-
bioRxiv - Neuroscience 2024Quote: Libraries for sequencing were prepared using the NEBNext Ultra II library prep kit (New England Biolabs) following the protocol and thermocycling temperatures therein ...
-
bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from RNA using ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs) using random primers (included with kit) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by processing with the NEBNext Ultra Directional RNA Sequencing for Illumina kit (New England Biolabs). Illumina sequencing ...
-
bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB, #E6560S) and 18-mer oligo dT primers ...
-
bioRxiv - Molecular Biology 2024Quote: ... The dsDNA was then linearly amplified using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) for 15Lh at 37L°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... were used to generate gRNA using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) and purified using the RNeasy Mini Kit (Qiagen).