Labshake search
Citations for New England Biolabs :
5101 - 5150 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: The pVG344 plasmid was cloned using the NEBuilder HIFI DNA Assembly Cloning Kit (New England Biolabs) to assemble four amplified fragments ...
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bioRxiv - Genetics 2020Quote: ... without modification except for library preparation performed with NEBNext® Ultra kit (New England Biolabs®). For library preparation 600 ng of each genomic DNA were fragmented by sonication and purified to yield fragments of 150-200 bp ...
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bioRxiv - Genetics 2020Quote: ... Both cloning steps were performed with the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The AarI-AarI fragment from pLdhGao23 was cloned into the AarI site of pScarless-dhGaoR ...
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bioRxiv - Cell Biology 2021Quote: ... mRNAs were isolated from total RNAs with Magnetic mRNA Isolation Kit (oligo(dT) beads) (NEB, E7490). First strand cDNAs were synthesized using random primers and second strand cDNAs were synthesized subsequently ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All ligations to generate this construct were done with the Quick Ligation™ Kit (NEB, M2200S). The final construct was sequence validated ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All ligations to generate this construct were done with the Quick Ligation™ Kit (NEB, M2200S) and the final construct was sequence validated ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The OdinTubulin mutation (H393D) was introduced using the Q5 Site Directed Mutagenesis Kit (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was subjected to rRNA depletion using the NEBNext rRNA Depletion Kit (New England Biolabs), according to the manufacturer’s protocol ...
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Plasma derived cell-free mitochondrial DNA originates mainly from circulating cell-free mitochondriabioRxiv - Genomics 2021Quote: ... DSP libraries were prepared with the NEB Next® Ultra™ II kit (New England Biolabs). SSP libraries were prepared with the Swift ACCEL-NGS® 1S PLUS kit (Swift Biosciences) ...
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bioRxiv - Genomics 2021Quote: ... Standard indexed Illumina libraries were prepared using the NEBNext DNA Library Prep kit (New England BioLabs), followed by amplification using KAPA HiFI DNA polymerase (KAPA Biosystems) ...
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bioRxiv - Genomics 2021Quote: ... UMI-4C sequencing libraries were generated using the NEBNext Ultra II library prep kit (NEB, E7645L). For each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... The libraries were amplified and indexed according to the standard protocol (small RNA sequencing kit, NEB), except that PCR conditions were adjusted in order to prevent library over amplification ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) and sequenced on the Illumina HiSeq 2500 platform ...
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bioRxiv - Neuroscience 2020Quote: ... Samples underwent methylated DNA depletion (MDD) using the NEBNext Microbiome DNA Enrichment Kit (New England Biolabs), and the enriched fraction was used for DNA library construction as above (Figure 1) ...
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bioRxiv - Microbiology 2021Quote: ... following the MICROBEnrich + RiboMinus™ Eukaryote Kit treatments were further treated with Poly(A) Polymerase (NEB), which adds a Poly(A ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Isolated RNA was reverse-transcribed into cDNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB) with either the enclosed Random Primer Mix or the M1-specific oligo M1_R3 (Table S3).
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bioRxiv - Cancer Biology 2021Quote: Site-specific mutation was performed using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs; #E0554S). EZH2 H689A mutation was using the primers F5’-CAAATGCTTCGGTAAATCCAAACTGC-3’ and R5’-CCGAAGCATTTGCAAAACGAATTTTG-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... cDNAs were synthesized using the ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs) and analyzed for the expression of the exosome production-related genes listed in Appendix Table 1 by RT-PCR on a CFX Connect Real-Time System (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2020Quote: ... Plasmids with mutated Marcksl1a and Marcksl1b were generated using Q5® Site-Directed mutagenesis kit (NEB). shRNA containing vectors were constructed by ligation of shRNA sequence between the EcoRI and PmeI sites downstream of the U6 promoter of modified pEGFP-U6 vector 36 (a gift from Zuoshang Xu ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequencing libraries were constructed with NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). The libraries were sequenced with Illumina Novaseq by Novogene Co ...
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bioRxiv - Cell Biology 2021Quote: ... followed by library preparation using NEBNext® Ultra™ II RNA Library Preparation Kit (NEB, E7770S). Libraries were paired-end sequenced with read lengths of 150 bp on an Illumina HiSeq X Ten instrument ...
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bioRxiv - Genetics 2020Quote: ... One was to use a kit to generate sequencing libraries in one-tube reactions (NEB, E7103S). Another modification was to spike-in the panel of synthetic nucleosomes carrying major histone methylations (EpiCypher ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was extracted using the “Monarch Total RNA Miniprep” kit (New England Biolabs, Ipswich, MA, USA) and then was reverse transcribed by random priming using the PrimeScript RT Reagent Kit (Takara Bio Europe ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated using the Monarch Total RNA Miniprep Kit (New England Biolabs, Whitby, ON) following manufacturer guidelines ...
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bioRxiv - Cell Biology 2021Quote: mRNAs were synthesized by in vitro transcription (IVT) using HiScribeTM T7 ARCA mRNA Kit (NEB, E2060) according to the manufacturer’s instruction with slight modification ...
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bioRxiv - Genetics 2021Quote: ... PCR products were purified using NEBMonarch PCR and DNA Clean-up Kit (New England Biolabs, USA), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... University of Cambridge) using the NEBuilder® HiFi DNA Assembly kit based on manufacturer’s instructions (NEB). Transgenic animals were validated by RT-PCR for correct insert ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed and RNA was isolated using the Monarch Total RNA Miniprep Kit (NEB T2010S), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... we used the Ampli1 ReAmp/ds kit and removed the adaptors with MseI (New England Biolabs). As per their recommendation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Linear plasmids were subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were purified using the Monarch PCR & DNA Cleanup Kit (New England BioLabs., Ipswich, MA, USA). Linearized plasmids ...
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bioRxiv - Neuroscience 2020Quote: ... Mutations were generated in the rat P2X2 receptor using the Q5 Site-Directed Mutagenesis Kit (NEB). Each mutation was verified by DNA sequencing and subcloned in pWPT-EF1α-P2X2-GCaMP6s-IRES-DsRed2 or pWPT-EF1α-P2X2-GCaMP6s-P2A-mScarlet lentiviral vectors.
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bioRxiv - Molecular Biology 2021Quote: ... The targeting construct was generated by Gibson assembly (Gibson Assembly Master Mix kit, New England Biolabs) of the PCR-amplified tag sequence and roughly 520bp homology arms surrounding the ATG start codon of the Med14 gene ...
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bioRxiv - Microbiology 2019Quote: Conventional RT-PCR was performed using Phusion® High-Fidelity DNA Polymerase kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Mutations into these plasmids were introduced using a Q5 site-directed mutagenesis kit (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... coli total RNA was purified using the Monarch® Total RNA Miniprep Kit (New England Biolabs) according to manufacturer’s instructions including the recommended on-column DNase treatment.
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bioRxiv - Microbiology 2019Quote: ... rpsLK43T and folAL28R mutated alleles by using the Q5 site-directed mutagenesis kit (New England Biolabs) and the primers listed in table S4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we generated Illumina libraries for each individual using the NEBNext Ultra II kit (New England Biolabs). For RADseq data ...
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bioRxiv - Immunology 2021Quote: ... before cDNA was synthesized with the AMV LongAmp® Taq RT-PCR kit (New England Biolabs). A 500bp fragment covering the second and third exons common to all polymorphic MHC class I genes as amplified from each cDNA using NEB Go Taq polymerase and ovine MHC class I generic primers 416 and Cr as described in36 ...
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries were generated by using NEBNext UltraII FS Library Prep Kit (New England BioLabs, USA), and QC of cDNA and final libraries was performed by MultiNA (Shimazu ...
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bioRxiv - Microbiology 2021Quote: ... Constructs with these individual mutations were generated using Q5 Site-Directed Mutagenesis Kit (New England Biolabs). A 2-step PCR protocol using the Q5 mutagenesis kit was used to join nucleotides 1-117 ...
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bioRxiv - Molecular Biology 2021Quote: ... BspEI and BclI restrictions sites were introduced using Q5® Site-Directed Mutagenesis Kit (NEB, E0554). The left homology arm was cloned using Gibson assembly strategy by linearizing the vector with BspEI (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... sgRNAs were generated using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs). Founder mice (F0 ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using Luna® Universal One-Step RT-qPCR Kit (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... DNA isolation from reaction mixtures was performed using Monarch PCR & DNA Clean-up Kit (NEB, USA). Plasmid miniprep DNA isolation was performed using Wizard Plus SV Minipreps DNA Purification System (Promega ...
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bioRxiv - Genomics 2021Quote: ... Libraries were generated using NEBNext®UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s ...
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bioRxiv - Genomics 2021Quote: ... and the library was generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA), following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2019Quote: ... The 19 base-sgRNA was cloned into pDD162 using a Q5 mutagenesis kit (New England Biolabs), as described by 48 ...
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bioRxiv - Genomics 2021Quote: ChIP libraries were prepared while bound to Dynabeads using NEBNext Ultra II Library preparation kit (NEB) using half reactions ...
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bioRxiv - Microbiology 2021Quote: Bacterial RNA was extracted using the Monarch total RNA miniprep kit (New England Biolabs, Ipswich, MA) following manufacture’s guidelines ...