Labshake search
Citations for New England Biolabs :
5151 - 5200 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Point mutations rescuing individual lysine residues were introduced using the Q5 Site-Directed Mutagenesis Kit (NEB) and confirmed by sequencing ...
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bioRxiv - Developmental Biology 2020Quote: ... Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cDNA NGS library preparation (NEBNext® Ultra RNA Library Prep Kit for Illumina, NEB). The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were quantified using a NEBNext® Library Quant Kit for Illumina (New England BioLabs, E7630L) and sequenced on either an Illumina NextSeq 500 instrument using 42bp paired-end reads ...
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bioRxiv - Microbiology 2022Quote: ... before preparation of stranded libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB). cDNA libraries were sequenced on an Illumina NovaSeq 6000 to generate ~55-130 million paired-end reads of 75 bp per sample (Supplemental Table 2) ...
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bioRxiv - Immunology 2022Quote: ... then cDNA libraries were generated using a NEBNext Ultra DNA Library Prep Kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: All fragments were assembled using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, #E5520S) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by Gibson assembly with the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs), was performed as described in Schaub et al ...
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bioRxiv - Biochemistry 2022Quote: In vitro coupled transcription-translation was performed at 37 °C using the PURExpress ΔIleRS Kit (NEB) following manufacturer’s protocol supplemented with [35S]-methionine (EasyTag ...
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bioRxiv - Genomics 2022Quote: ... SNVs were inserted into the regions of interest using the Q5 Site-Directed Mutagenesis Kit (NEB) and mutation-specific primers were designed with the NEBaseChanger tool ...
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bioRxiv - Neuroscience 2022Quote: DNA was purified using the Monarch PCR and DNA cleanup kit (New England Biolabs, 13-0041) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... gDNA was extracted using the NEB Monarch gDNA extraction kit and digested overnight with DpnI (NEB). DamID adaptors were then ligated to DpnI-digested DNA ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression was determined using SYBR green Luna One Step RT-qPCR Kit (New England BioLabs) on a C1000 Touch (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Quenched IVT reactions were prepared using the Monarch® RNA cleanup kit 500μg (New England Biolabs), and the concentration of the mRNA eluted in 50μl was determined using the Quant-iT™ broad range RNA assay.
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (NEB), with the insert size in the range of 150 to 250 bp ...
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bioRxiv - Bioengineering 2022Quote: ... pooled libraries were quantified using the NEBNext Library Quant Kit (New England Biolabs, Ipswich, MA, USA) for Illumina.
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bioRxiv - Microbiology 2022Quote: ... In vitro translation reactions were performed using PURExpress ΔRF123 kit and PURExpress ΔRibosome (New England Biolabs) according to manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: ... The assembled product was purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L) and eluted in 12 μl of H2O.
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bioRxiv - Biochemistry 2022Quote: ... All VPS37A1-148 mutants were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). The corresponding primers are listed in Supplementary Table 2 and all constructs were verified by sequencing ...
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bioRxiv - Bioengineering 2022Quote: ... the reaction was cleaned and concentrated with a Monarch PCR & DNA Cleanup Kit (NEB catalog # T1030L) and eluted in either 10 or 12 µL of nuclease free H2O ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA was extracted using the Monarch® Total RNA Miniprep Kit (T2010S, New England Biolabs), and cDNA was generated using the SuperScript™ IV First-Strand Synthesis System (18091050 ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA synthesis was performed with random primers from the Protoscript cDNA synthesis kit (NEB Cat#E6560S). Transgene expression was then quantified via qPCR using the iTaq Universal SYBR green supermix and primers binding to the mCherry transcript ...
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bioRxiv - Genomics 2022Quote: ... The PCR product was purified using the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L). For the last PCR ...
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bioRxiv - Genomics 2022Quote: ... The product was then purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L), and eluted with 20 μl of ddH2O ...
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bioRxiv - Developmental Biology 2022Quote: CUT&RUN libraries were constructed using the NEB Ultra II DNA library prep kit (NEB #E7645) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... cleaned up A-tailed DNA went through Adaptor ligation reaction using Quick Ligation Kit (NEB, M2200L) following manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Illumina sequencing libraries were made using the NEBNext Ultra II RNA Library Prep Kit (NEB #E7775). The quality of the libraries was assessed using an Agilent Bioanalyzer DNA High Sensitivity chip ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was carried out using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) and were paired-end sequenced (38bp+38bp ...
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bioRxiv - Genetics 2022Quote: ... Illumina libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... RNA was subsequently isolated following the manufacturer’s instructions of the Monarch RNA Isolation Kit (#T2010S; NEB). RIN scores and concentration were performed using High Sensitivity RNA ScreenTape (#5067-5579/#5190-6507 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Gibson assemblies were performed using the NEB HiFi Assembly Kit (New England Biolabs, Cat. No. E2621L) with 100 ng of insert and a 2:1 molar ratio of insert:vector ...
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bioRxiv - Developmental Biology 2022Quote: ... Ac5-DanFL was made by DNA assembly using NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S) from pAc5.1C-FLuc-V5His6 (Addgene plasmid #21183) ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg was used to generate dsRNA using a HiScribe T7 RNA synthesis kit (NEB). Reaction products were purified with the GeneJET RNA Purification Kit (ThermoFisher) ...
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bioRxiv - Plant Biology 2022Quote: mRNA libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina (NEB, USA) according to the instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were generated using the NEBNext® Ultra ™ RNA Library Prep Kit (NEB, USA). After library construction ...
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bioRxiv - Synthetic Biology 2022Quote: ... The sequencing library was prepared using NEBNext Ultra II FS DNA library prep kit (NEB E7805L) with 500 ng genomic DNA as input ...
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bioRxiv - Plant Biology 2022Quote: One μg of RNA was reverse transcribed using the LunaScript RT SuperMix Kit (New England Biolabs) according to the manual ...
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bioRxiv - Physiology 2022Quote: ... Total RNA was purified with the Monarch RNA Cleanup kit (New England Biolabs, Ipswich, MA, US).
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bioRxiv - Molecular Biology 2022Quote: ... and genomic DNA was extracted by using a Monarch Genomic DNA Purification Kit (New England Biolabs). The thyA gene region was amplified from the genomic DNA (5 ng ...
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bioRxiv - Molecular Biology 2022Quote: ... and genomic DNAs were extracted by using a Monarch Genomic DNA Purification Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA libraries were constructed using the NEBNext Ultra II FS DNA Library Prep kit (NEB, USA) and sequenced using the Illumina HiSeq X Ten platform.
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bioRxiv - Microbiology 2022Quote: ... For the MksB hydrolysis mutant (MksBE1042Q) a site-directed mutagenesis (Q5 site-directed mutagenesis Kit, NEB) was performed using primer pair MG060/MG061 and pMG004 as template ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared using a NEBNext Ultra II fragment library preparation kit (New England Biolabs, USA) with Covaris shearing (Covaris ...
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bioRxiv - Microbiology 2022Quote: ... Purified RNA then was pulldown using m6A specific antibody using EpiMark N6-methyladenosine enrichment kit(NEB). RNA was converted into cDNA using random hexamer primers and Revert aid RT (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... The libraries preparation was performed with Ultra II RNA Library Prep Kit for Illumina (NEB, E7770) and Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2022Quote: ... and library construction was executed using NEBNext Ultra RNA Library Prep kit (New England Biolabs, USA), and sequenced on a NovaSeq6000 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by library preparation using NEBNext® Ultra™ II RNA Library Preparation Kit (NEB, E7770S). Libraries were paired-end sequenced with read lengths of 150 bp on Illumina HiSeq X Ten or Nova-seq instruments ...
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bioRxiv - Molecular Biology 2022Quote: ... The final pool was quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, E7630L) and paired-end sequenced on an Illumina MiSeq.
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bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were prepared with a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced on an Illumina HiSeqX10 platform using a 150-bp paired-end sequencing strategy at Novogene Co. ...
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bioRxiv - Neuroscience 2022Quote: ... RNAs were used to synthesize cDNAs using the ProtoScript cDNA synthesis kit (New England Biolabs, USA) and subjected to RT-PCR analyses to determine STAU1-RNA interactions ...