Labshake search
Citations for New England Biolabs :
5051 - 5100 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... An Illumina-compatible sequencing library was generated (NEBNext Ultra II DNA Library Kit, New England Biolabs) for each biological replicate and sequenced using the NextSeq 500 at the University at Albany Center for Functional Genomics.
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bioRxiv - Genomics 2019Quote: ... and libraries were constructed with NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). The fragment size distribution of each library was characterized with a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Genomics 2019Quote: ... The four-way assembly reaction was performed using NEBuilder HiFi DNA Assembly Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... and sequencing libraries were prepared using the NEBnext Ultra RNA library prep kit for Illumina (NEB). Completed libraries were sequenced on a NextSeq 500 sequencer (Illumina ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were generated from genomic DNA with a Phusion® High-Fidelity PCR kit (NEB) and primers that introduced KpnI and XhoI restriction sites ...
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bioRxiv - Immunology 2020Quote: ... and sequencing libraries prepared using the NEB Next Ultra II Directional RNA kit (NEB, Ipswich, MA) then sequenced to a depth of at least 20 M single-end 75 bp reads using NextSeq 500 (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... the sequencing library was prepared using the NEBNext® Multiplex Small RNA Library Prep Kit (NEB) and the resulting library was loaded onto an Illumina HiSeq X machine for PE150 sequencing.
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bioRxiv - Genomics 2019Quote: ... processed with the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, E7770), followed by deep sequencing analysis using Illumina HiSeq X Ten platform (2× 150-bp paired end ...
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bioRxiv - Immunology 2020Quote: ... PCR was performed with a Phusion High-Fidelity PCR Kit (New England Biolabs, Ipswich, MA, USA) in 20µL reactions mixed with 2µL cDNA and 10µM primers ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA was reverse transcribed using First Strand cDNA Synthesis Kit and amplified using Phusion polymerase (NEB) with primers for the relevant genes (see Table S2) ...
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bioRxiv - Genetics 2019Quote: ... for each sample RNA was first depleted of rRNA using the NEBNext rRNA Depletion kit (NEB). RNA-seq libraries were then prepared from 200 ng of RNA using the NEBNext Ultra II Directional RNA-seq kit (NEB) ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR product was purified using Monarch® Nucleic Acid Purification Kit (New England Biolabs Inc.).
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Combinations of maternal-specific repressive epigenetic marks in the endosperm control seed dormancybioRxiv - Molecular Biology 2020Quote: mRNA was isolated from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB) and cDNA libraries were constructed using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, #E7420S). Sequencing was performed by LAFUGA on an Illumina Hiseq 1500 using 50 bp paired end run.
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bioRxiv - Molecular Biology 2020Quote: Library preparation was performed using the Ultra II DNA Library prep kit for Illumina (NEB, #E7645S) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2020Quote: ... and DNA assembly with NEBuilder kit were done according to the manufacturer recommendations (New England Biolabs). DNA sequences of constructs were confirmed by sequencing with the appropriate primers ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplified products were cloned using the NEB PCR cloning kit (New England Biolabs, Ipswich, MA). Clones from each founder were Sanger sequenced using the NEB analysis primers to define indels.
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bioRxiv - Cell Biology 2020Quote: ... gel purified and ligated with pENTR (w158-1)-backbone by Quick Ligation kit (New England Biolabs) to create pENTR-GRET ...
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bioRxiv - Synthetic Biology 2019Quote: ... transcription templates were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of the plasmids to include a 5’ region upstream of the T7 promoter and a 3’ region ending with either 3WJdB or the T7 terminator with the primers listed in Supporting Data File 1 ...
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bioRxiv - Genomics 2020Quote: ... followed by DNA clean-up using the Monarch PCR & DNA Cleanup Kit (New England BioLabs T1030S). Library preparations were quantified using the using KAPA library quantification kit (Kapa Biosystems KK4824 ...
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bioRxiv - Microbiology 2020Quote: ... and PCR mixes using the Q5® High-Fidelity PCR Kit (New England Biolabs, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... The mutant was generated using the Q5 ® Site-Directed Mutagenesis kit (NEB, catalog number #E0554S) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2020Quote: ... Library construction was done using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and subject to library preparation using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB). Libraries with barcodes were pooled and sequenced on the Illumina HiSeq 1500 platform ...
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bioRxiv - Molecular Biology 2020Quote: DNA libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) with index primers (NEB ...
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bioRxiv - Synthetic Biology 2020Quote: ... cDNA was synthesized from 500ng RNA using the Protoscript II First Strand cDNA synthesis Kit (NEB). 1ul of cDNA was amplified by PCR with primers that amplify about 200 bp surrounding the sites of interest using OneTaq PCR Mix (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA libraries were generated from RNA using the NEBNext Ultra Directional RNA Library Preparation kit (NEB). Samples were sequenced by 75 bp paired-end sequencing using a NextSeq 500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina® (NEB, USA). The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina) ...
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bioRxiv - Synthetic Biology 2020Quote: ... cDNA was synthesized from 500ng RNA using the Protoscript II First Strand cDNA synthesis Kit (NEB). 1ul of cDNA was amplified by PCR with primers that amplify about 200 bp surrounding the sites of interest using OneTaq PCR Mix (NEB) ...
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bioRxiv - Genetics 2021Quote: ... DNA libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB# E7645), using Ampure XP beads for cleanup ...
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bioRxiv - Molecular Biology 2021Quote: IVT was carried out using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB E2050S) with the addition of RNAsin (Promega N2111) ...
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bioRxiv - Molecular Biology 2021Quote: ... We prepared cDNA from 1μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB, #E3010). No Template and No Reverse Transcriptase controls (NTC and NRT ...
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bioRxiv - Microbiology 2020Quote: ... processed the extracted DNA with the NEBNext Microbiome DNA Enrichment Kit (New England Biolabs, Ipswich, MA), and quantified the resulting DNA using Picogreen (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: β1-tubulin wild type construct was generated by the gibson assembly (HiFi Kit, New England Biolabs) of a TubB1 sequence fragment synthesised as a gBlock by Integrated DNA Technologies (IDT ...
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bioRxiv - Synthetic Biology 2020Quote: ... GLuc activity in samples was measured using the Biolux Gaussia Luciferase Assay kit (NEB, Ipswich, MA). GLuc assay solution (50 μL ...
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bioRxiv - Genetics 2020Quote: ... We prepared the library using NEB Ultra II Directional RNA library kit for Illumina (NEB E7760) used dual index primers (from NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... mRNAs for PARE libraries were isolated using the NEB Magnetic mRNA Isolation Kit (New England Biolabs), custom adapters for selecting 5’-P mRNAs and primers from the TruSeq Small RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... mRNAs for RNA libraries were isolated using the NEB Magnetic mRNA Isolation Kit (New England Biolabs). RNA libraries were produced using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... thlaspeos was extracted using the NEB Monarch Genomic DNA Purification Kit (New England Biolabs, Frankfurt, Germany).
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bioRxiv - Genomics 2019Quote: ... We inserted the minimal promoter and GFP using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) as described above.
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bioRxiv - Physiology 2020Quote: ... Prior to stranded mRNA isolation library construction using NEBnext library kits (New England Biolabs, Evry, France), the quality of the RNA pool was assessed on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Plant Biology 2019Quote: ... and libraries were prepared using NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs) followed by size selection (~320 bp ...
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bioRxiv - Biophysics 2019Quote: ... Mutants of skRLC and smRLC were obtained by Q5 site-directed mutagenesis kit (New England BioLabs) in pET3a vector ...
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bioRxiv - Genetics 2019Quote: ... were generated with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Illumina stranded and paired-end (PE ...
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bioRxiv - Biochemistry 2019Quote: ... HSPA1 R469A and HSP8 R469A mutants was performed using Q5® Site-Directed Mutagenesis Kit (NEB), following manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Reverse transcriptase was performed with the ProtoScript II First Strand cDNA synthesis kit (New England BioLabs) using 1µg of total RNA with random hexamer primers ...
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bioRxiv - Evolutionary Biology 2019Quote: Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... One-step qRT-PCR was performed using the Luna Universal One-Step RT-qPCR Kit (NEB) and 5 ng RNA per reaction in technical duplicate with a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... ribosomal RNA depletion was performed using 1 μg RNA using NEBNext rRNA Depletion kit (NEB#6310). Concentration of final libraries was measured using Qubit 2.0 Fluorometer in combination with Qubit dsDNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Libraries were generated with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). Briefly ...