Labshake search
Citations for New England Biolabs :
5001 - 5050 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... 5 µl from the PCR product were circularized using 1 µl T4 DNA ligase and 2 µl ligation buffer 10x (NEB) in a final volume of 20 µl ...
-
bioRxiv - Bioengineering 2024Quote: ... All PCR amplifications were performed using the Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA).
-
bioRxiv - Bioengineering 2024Quote: ... The TSA1 promoter was amplified by PCR from genomic DNA obtained from the X-33 strain using Q5 polymerase (New England Biolabs) and primers TSA1_BstBI_Fw and TSA1-GFP_Rv_KpnI ...
-
bioRxiv - Biochemistry 2023Quote: ... the MsbA gene (from Escherichia coli genomic DNA) was amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity DNA Polymerase (New England Biolabs, NEB) and subcloned into a modified pCDF-1b plasmid (Novagen ...
-
bioRxiv - Biochemistry 2024Quote: ... The on- and off-target genomic sites (experimentally determined from a previous study) were PCR amplified with Phusion plus DNA polymerases (New England Biolabs) and locus-specific primers having tails complementary to the Truseq adapters ...
-
bioRxiv - Bioengineering 2024Quote: ... and mCherry inserts were generated via PCR and cloned into the linearized viral backbone as a single fusion protein using HiFi cloning mix (NEB). H2B-iRFP nuclear marker was (Addgene Plasmid #90237) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Adapter sequences were then removed by restriction digest (PCR reaction product, 1X rCutSmart NEB Buffer, 5 U EcoRV-HF (NEB)) at 37°C for 1 hour ...
-
bioRxiv - Cancer Biology 2024Quote: ... Adapter sequences were then removed by restriction digest (PCR reaction product, 1X rCutSmart NEB Buffer, 5 U EcoRV-HF (NEB)) at 37°C for 1 hour ...
-
Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... the sgRNA regions were amplified from the genomic DNA by PCR using the NEBNext Q5 HotStart HiFi polymerase (NEB, M0543) and specific primers ...
-
bioRxiv - Microbiology 2024Quote: ... and adapter sequences with unique indexes were inserted with 15 cycles of PCR using NEB Next single index adapters (New England BioLabs). Purified PCR products were measured and pooled for sequencing on an Illumina NextSeq 550 High Output Flow Cell using the single-end 75 base technique using AMPure XP SPRI beads (Beckman Coulter Life Sciences).
-
bioRxiv - Neuroscience 2024Quote: ... Subcloning PCR product and pEGFP-C1 backbone were cut using Kpn1-HF and Spe1-HF restriction enzymes (New England BioLabs) and ligated using Quick Ligase Kit (New England BioLabs ...
-
bioRxiv - Molecular Biology 2024Quote: ... the double-stranded (ds) cDNA was PCR amplified with primers directed against 5’ and 3’ RNA cassettes and NEB Q5 HotStart polymerase (NEB). To introduce unique barcodes secondary PCR was performed using TrueSeq primers (NEB ...
-
bioRxiv - Microbiology 2024Quote: ... PCRs were done in a 50 µl mix for 30 cycles using Phusion High-Fidelity DNA polymerase (New England Biolabs). Purification of PCR products was routinely performed using the QIAquick PCR Purification Kit (Qiagen ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and Caspase-1 (Provided by Peter Broz) 39 were generated via PCR and inserted into the pHR backbone via HiFi cloning mix (New England Biolabs). All Melt37/40-Effector fusions were generated by amplifying Melt37/40 with primers that amplified the region downstream of a.a.96 such that the final Melt variants contained a a.a.1-96 deletion ...
-
bioRxiv - Immunology 2024Quote: ... The remainder of the adapter sequences were added to the heavy and light chains separately by a two-step PCR reaction with Q5 using the NEBNext index primers (NEB) 98°C for 30s ...
-
bioRxiv - Neuroscience 2024Quote: ... the T2A-mCherry sequence was amplified by PCR and added after the second NLS sequence using the NEBuilder HiFi Assembly Master Mix (New England Biolabs). A previously described sgRNA sequence targeting Shank3 (Supplementary Table 2 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Synthetic genomes were in vitro assembled using PCR-generated fragments and Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, BioLabs) with template DNA consisting of φ2638A WT gDNA that had been circularized through annealing of the terminal cos sites by heating gDNA to 65°C for 10 mins ...
-
bioRxiv - Plant Biology 2024Quote: ... and GFP-HSPTerminator amplicon from pMod_C3003 vector were amplified by PCR and assembled by using NEB HiFi assembly master mix (E2621S, NEB, USA). Later ...
-
bioRxiv - Neuroscience 2024Quote: ... The resulting fragments were amplified using ligation-mediated polymerase chain reaction (LM-PCR) with Q5 Hot Start High-Fidelity 2X Master Mix (NEB) to allow the addition of homology arms necessary for cloning ...
-
bioRxiv - Neuroscience 2024Quote: ... The final cDNA sample was then split into 16 separate reactions for final PCR amplification using 16 unique Illumina indexing primers and Q5 Hot Start High-Fidelity 2X Master Mix (NEB). After amplifications ...
-
bioRxiv - Molecular Biology 2024Quote: ... were generated by extending a common sgRNA(F+E) sequence with gene-specific crRNA sequence downstream of a T7 promoter by PCR with Phusion polymerase (NEB). PCR product was gel extracted and sgRNA was in vitro transcribed using the MegaShortScript™ T7 Transcription Kit (Invitrogen AM1354 ...
-
bioRxiv - Neuroscience 2024Quote: Total RYR1 transcripts were amplified as 7 overlapping PCR products.38 They were sequenced after fragmentation and library preparation using NEBNEXT NGS workflow (New England Biolabs) according to manufacturer recommendation ...
-
bioRxiv - Microbiology 2024Quote: ... Genomic DNA from 12 of the clones was extracted and mutations were verified by PCR using OneTaq polymerase (New England Biolabs) with the appropriate primers (supplementary methods ...
-
bioRxiv - Pathology 2024Quote: ... Prior to transformation the bait plasmids were linearised in the URA3 gene by PCR or restriction digest (BbsI/Esp3I/BstBI (NEB)) to allow integration into the genome ...
-
bioRxiv - Developmental Biology 2024Quote: ... bulk-extracted genomic DNA from these strains was used to set up PCR reactions for each primer set using Quick Load Taq 2X Master Mix (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... 50 ng cDNA was amplified by PCR for 30 cycles using Phusion High Fidelity DNA Polymerase (M0530L; New England Biolabs) using the following primers:
-
bioRxiv - Microbiology 2024Quote: ... Libraries were then size selected for cDNA target fragments of 200–300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... PCR-enrichment was performed in 18 cycles in ten 10μL reactions per sublibrary with Phusion® HF PCR Mastermix (New England BioLabs) by mixing 5µl 2× Phusion Master mix ...
-
bioRxiv - Evolutionary Biology 2024Quote: DNA fragments for wild-type and mutant GART were ordered from IDT (see below) and PCR amplified with Phusion DNA polymerase (New England Biolabs) with FWD_adapter and REV_adapter primers from IDT (see below) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... One microliter was used as DNA matrix to produce PCR amplicons with the Phusion High Fidelity Taq Polymerase (#M0530; NEB) and new primers (Salten-pb1-3183-F and Salten-pb1-4136-R ...
-
bioRxiv - Evolutionary Biology 2024Quote: Indexed libraries were generated using the standard Illumina two-step PCR protocol using Q5 high fidelity DNA polymerase (New England Biolabs). Paired-end sequencing with a 2×250 bp read length was performed at the Bio-Environment platform (University of Perpignan Via Domitia Perpignan ...
-
bioRxiv - Developmental Biology 2024Quote: ... Sox17 and sox32 deletion and domain switch constructs (Table S1) were generated using pCS2+sox17 and pCS2+myc-sox32 by PCR amplification using Q5 polymerase (NEB) using described primers (Table S2 ...
-
bioRxiv - Developmental Biology 2024Quote: ... 8 injected embryos and 8 control uninjected siblings were assayed by PCR amplification and T7 endonuclease (New England Biolabs, M0302S) digest for mutation analysis41 ...
-
bioRxiv - Genetics 2024Quote: ... DNA was isolated from HepG2 cells and the regions were amplified with PCR primers containing restriction enzyme (RE) cutting sites for NsiI (New England Biolabs, NEB) and BamHI/HindIII (NEB ...
-
bioRxiv - Genomics 2024Quote: ... were amplified by PCR and cloned at the ClaI site in the Rosa26-targeting vector pEN111 by Gibson assembly (New England Biolabs). The resulting vectors obtained (pEN111-TRE3G-LKF/AKF-IRES-ZsGreen1-rtta3G_Rosa26-donor ...
-
bioRxiv - Cell Biology 2024Quote: ... 100 fmol of library per 50 µL PCR reaction was amplified using Q5 HotStart DNA Polymerase (New England Biolabs M0493S) in 8 reactions with a 50 °C – 62 °C gradient annealing step using the following program:
-
bioRxiv - Microbiology 2024Quote: ... 2 kb fragments upstream and downstream of SrcF were PCR amplified with Q5 High Fidelity DNA Polymerase (New England Biolabs) and cloned in PCR amplified pEx-deletion-ermG via DNA Gibson assembly (HiFi DNA Assembly Master Mix ...
-
bioRxiv - Immunology 2024Quote: ... Two microliters were subsequently used in real-time quantitative PCR reactions containing SYBR-green based Luna universal dye qPCR mix (New England Biolabs) and gene specific PCR primers ...
-
bioRxiv - Immunology 2024Quote: ... 10 ng of restriction enzyme digested backbone and 20 ng of SPRI purified PCR variable regions were combined with an appropriate volume of 2x HiFi master mix (New England Biolabs) for one hour at 50°C ...
-
bioRxiv - Microbiology 2024Quote: The barcode-containing region was amplified from the bacterial DNA using the boiled bacterial cells in a PCR with OneTaq HS Quick-Load Master Mix (New England Biolabs) and custom primers (Supplemental Data 3) ...
-
bioRxiv - Microbiology 2024Quote: Plasmids were constructed by amplifying inserts by PCR using specific oligonucleotides and the Q5 high fidelity enzyme (New England Biolabs). PCRs were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel) ...
-
bioRxiv - Microbiology 2024Quote: ... Libraries were then size-selected for cDNA target fragments of 200–300 bp on 2% Low Range Ultra Agarose followed by PCR amplification using Phusion DNA polymerase (NEB) for 15 PCR cycles ...
-
bioRxiv - Microbiology 2024Quote: ... Strains were typically constructed by PCR amplifying vector and insert fragments with compatible 25-30 bp overhangs and ligating them by Gibson assembly (NEB). For the alt.-3-targeting pCas9 construct ...
-
bioRxiv - Microbiology 2024Quote: ... Primers with overhanging sequences homologous to either 5’ or 3’ end of target gene fragments were used to linearize pMCSG53 expression vectors at the multiple cloning sites through PCR reactions (Q5 High-Fidelity 2X Master Mix, New England Biolabs). Amplicons were subsequently gel-extracted (Wizard SV Gel and PCR Clean-Up System ...
-
bioRxiv - Microbiology 2024Quote: ... The relevant regions were amplified by PCR using primers designed for this study and shown in Table S1 Q5 High Fidelity DNA polymerase (NEB) was used for sequence analysis with appropriate purity and accuracy.
-
bioRxiv - Microbiology 2024Quote: ... and used as a template for barcode amplification in a 1-step PCR with Q5 polymerase with high-GC buffer (NEB). Indexed samples were sequenced on a NextSeq 550 in high-output mode (Donnelly Centre ...
-
bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated by amplifying sgRNA sequences from genomic DNA using bar-coded Illumina-compatible adapter-containing primers and NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs). PCR products were pooled and purified with a ZymoSpin V column with Reservoir (Zymo Research) ...
-
Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... homology arms flanking the region to be deleted were obtained using polymerase chain reaction (PCR) and cloned into the pExG2-KanR suicide vector using Hi-Fi Assembly (New England Biolabs)70 ...
-
bioRxiv - Genetics 2023Quote: SRR2 and SRR107 were amplified from a Sox2 BAC (RP23-274P9) by PCR using Phusion polymerase (New England Biolabs E0553S) and cloned into the pJET1.2/blunt cloning vector (Thermo Fisher K1232 ...
-
bioRxiv - Genetics 2023Quote: ... The presence of infectious rIBV in the allantoic fluid was confirmed by a two-step reverse transcription polymerase chain reaction (RT-PCR) protocol using Protoscript II reverse transcriptase (NEB) and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen ...