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Citations for New England Biolabs :
5001 - 5050 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of purified DNA of each template including the T7 promoter at the 5’ end was used following the manufacturer instructions (HiScribe T7 High Yield RNA Synthesis kit, NEB). Primers used are listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... All hybrid constructs were tested for expression and cloned into the pcDNA5/FRT/TO-N-FLAG-hBirA* using restriction enzymes: 5’ KpnI (NEB) and 3’ NotI (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ChIP-seq libraries were prepared using 2-5 ng of input and ChIP samples and the kit NEBNext Ultra DNA Library Prep for Illumina (New England Biolabs) following manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Cells were then spun down at 500 g for 5 minutes and washed with 900 μL of NEBuffer 2.1 (New England Biolabs #B7202). The cells were pelleted (500 g for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... The DNA was digested overnight at 37°C with gentle agitation after adding 25 µl of 10x NEBuffer3 and 5 ul of 25 U/ul MboI (NEB). To biotin end-fill the DNA a 50 ul master mix containing the following was added ...
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bioRxiv - Biochemistry 2023Quote: Both native and modified oligonucleotides were 5’-32P labeled by [γ-32P]-ATP using T4 polynucleotide kinase (New England Biolabs) following manufactures recommendations ...
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bioRxiv - Genomics 2023Quote: ... we amplified the library using PCR with a 10 µl reaction mixture containing 5 µl of Phusion High Fidelity MASTER Mix (New England Biolabs), 2 µl of P1 primer (AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC G) ...
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bioRxiv - Cell Biology 2023Quote: ... media in each well was aspirated and cells were incubated in 80 μL of 1x Hanks’ balanced Salt Solution with 20 mM HEPES and 10 μL 100 μM Coelenterazine-400a diluted in PBS for 5 minutes before adding either 10 μL of 5.5 U of Enterokinase (New England Biolabs #P8070S) in PBS or 10 μL of vehicle PBS ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested and lysed as described above and were run over 5 ml of Amylose affinity resin (New England Biolabs), washed with 100 ml of 20 mM sodium phosphate pH 7.2 ...
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bioRxiv - Microbiology 2024Quote: The 5’ end of the sigAb transcript was identified using 5’ RACE following manufacturer’s protocol with template switching RT enzyme mix (NEB; M0466). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was then amplified for 5 cycles with ATAC index primers and NEBNext Ultra II Q5 Master Mix (NEB #M0544). Additional amplification cycles were then determined by qPCR using 5uL of original amplification with PerfeCTa SYBR Green FastMix Reaction Mixes (Quantabio 95072-012) ...
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bioRxiv - Molecular Biology 2024Quote: ... The final HiC library was generated with 5 PCR cycles using the NEBNext Ultra II DNA Library Prep Kit and NEBNext Dual Index primers (NEB) for Illumina sequencing ...
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bioRxiv - Microbiology 2024Quote: ... were cloned in the EcoRI-BamHI digested and gel purified pTCV vector with 5 000 units of T4 DNA ligase (HC, New England Biolabs) incubated at 16°C overnight ...
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bioRxiv - Immunology 2024Quote: ... in the HA head (H3 numbering 52-263) of A/Sydney/5/2021 was assembled using golden gate cloning (BsmBI-v2 Golden Gate Mix, NEB) with a barcoded segment into pDM_RevGen068 ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µM purified SNAP-tagged proteins were reacted with 10 µM biotin-conjugated benzylguanine (BG-bio) (S9110S; New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Genomics 2024Quote: ... CHART-enriched DNA was eluted twice in 200 μL of elution buffer supplemented with 5 U/μL RNase H (New England BioLabs) at room temperature for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... was mixed with an 80-mer spike-in RNA oligonucleotide internal standard (DNA and RNA oligonucleotide sequences in Table S5).5 The mixture was dephosphorylated shrimp alkaline phosphatase (rSAP, NEB) in NEB T4 RNA ligase buffer at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... In vitro RNA transcription was performed in 50 µl reactions: 5 µl RNAPol Reaction Buffer (New England BioLabs, Ipswich, MA), 2 µl T7 RNA Polymerase (New England BioLabs ...
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bioRxiv - Biochemistry 2024Quote: ... 1-5 mL of cell pellet was harvested for plasmid extraction with the Monarch Plasmid DNA Miniprep Kit (New England Biolabs) (T1010) ...
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bioRxiv - Biochemistry 2024Quote: ... When relevant cmpRNA (500 pmol) was 5’-end phosphate-32 “hot” labeled using fresh gamma phosphate-32 labeled ATP (50 nmol) and PNK (NEB) following the producer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... 3’ and 5’ ends were modified accordingly and the respective adapters were ligated with a T4 RNA ligase I (New England Biolabs). The final products were size-selected one last time and rRNA depletion was performed using custom-made biotinylated oligos(38 ...
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bioRxiv - Neuroscience 2024Quote: ... after removing the Gel Coverslip, slides were incubated in 5 ml warmed Clearing Solution (ref. 20300003, Vizgen) with Proteinase K (ref. P8107S, NEB) in a sealed petri dish for 24 hours at 37°C.
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bioRxiv - Microbiology 2024Quote: ... 5 µL of BL21 competent cells (or, in the case of the disulfide stabilized cspg binders, T7 shuffle express) (NEB) were dispensed onto the 1 µL reactions ...
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bioRxiv - Plant Biology 2024Quote: ... the full length coding sequences of cowpea VuLHY1 (Vigun10g1533300) and VuLHY2 (Vigun09g004100) were amplified from a 5’ RACE cDNA library using the Q5 High-Fidelity DNA polymerase (NEB) and specific primers (Table S1) ...
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bioRxiv - Physiology 2024Quote: ... Stranded mRNA-Seq library construction was carried out using 5 μL of purified mRNA (15-75ng) and NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). First and second strand cDNA synthesis ...
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bioRxiv - Biochemistry 2024Quote: ... Purified RNA redissolved in 5 µl of 10% (v/v) DMSO was combined with 1x T4 RNA Ligase Buffer (New England Biolabs), 0.5 U/µl SuperaseIN (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... The purified PCR was digested overnight at 37°C in a 120 μL reaction (5 μL BsaI-HF (NEB R3733L), 5 μL BlpI ...
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bioRxiv - Cancer Biology 2024Quote: ... Site-directed mutagenesis of SOX10 5’UTR was performed using Q5® Site-Directed Mutagenesis Kit (New England Biolabs, #E0554) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Bead-bound microglial nuclei were resuspended in 25 µL Antibody Incubation Buffer (995 µL Wash Buffer with 5 µL 200x BSA [B9000S, New England Biolabs]).
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bioRxiv - Biochemistry 2024Quote: ... Run-off transcription was then performed using 5 ng of PCR amplified template (HiScribe T7 High Yield RNA Synthesis kit; NEB), followed by DNase treatment (TURBO DNase ...
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bioRxiv - Biophysics 2024Quote: ... Positively supercoiled DNA was prepared by incubating with 9°N Reverse Gyrase (5 units/μg DNA, M0200, provided by New England Biolabs) in 35 mM Tris-HCl (pH 7.5 at 25 °C) ...
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bioRxiv - Cell Biology 2024Quote: The full-length open reading frame of SYP-5 was synthesized as a gBlock (IDT) and cloned into pMAL (New England Biolabs) to express Maltose-binding protein (MBP)-tagged SYP-5 with a 6xHis tag at the N-terminus ...
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bioRxiv - Cell Biology 2024Quote: ... the cDNA library was cleaned using the Monarch PCR & DNA Cleanup Kit (5 μg) (T1030L, New England Biolabs, MA, USA) using the 7:1 ratio of binding buffer:sample as per the manufacturer’s instructions and was eluted in 27.5 μL nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 μL of the product was transformed by heat shock into 10-beta competent cells (New England Biolabs, C3019I). Cells were then plated on agarose plates (supplemented with ampicillin 100 μg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 μL of the product was transformed by heat shock into 10-beta competent cells (New England Biolabs, C3019I). Cells were then plated on agarose plates (supplemented with ampicillin 100 μg/mL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... of which 5 μl was processed with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) with published modifications to the manufacturer’s protocol (Batty et al ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer, 1 µL T4 PNK, NEB, 1 µL γ-32P-ATP, Hartmann, 6 µL ddH2O) for 40 min at 37°C ...
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bioRxiv - Physiology 2024Quote: ... 5 flies were sorted into sterile microcentrifuge tubes and homogenised in 200 µl lysis buffer (1 X TE, 1 % Triton X-100, 1/100 proteinase K (NEB, P8107S)) using a mechanical pestle ...
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bioRxiv - Bioengineering 2024Quote: ... and phosphorylated (1 μL 100 μM F oligo, 1 μL 100 μM R oligo, 1 μL T4 ligase buffer (NEB, B0202S), 1 μL T4 PNK (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... with a 1:1 combination of oligodT 18 primers and random hexamers (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl 10× T4 PNK buffer and 1 μl T4 PNK enzyme (NEB) at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μl of BsaI-HFv2 and 1 μl of T4 DNA ligase (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... RNase H diluted (1:100) in 1× RNase H buffer (New England Biolabs) was added to the cells and incubated for 2 h at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... 120 nM fluorescent beacon and 1 U.μL-1 murine RNase Inhibitor (NEB M0314) in the reaction buffer containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: ... 1 μl of DNAse I at 1 mg/ml (NEB, cat no. M0303L), 1 μl RNAse at 10mg/ml (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The reaction was further adjusted with dTTP (1 µl, 1 mM) (NEB #N0443S), TdT reaction buffer (1 µl) ...
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bioRxiv - Biophysics 2024Quote: ... a 1 μL portion of a 1 mg/mL stock of trypsin (NEB Trypsin-ultraTM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the resulting cell pellet was resuspended in 1 mL of RNA Protection Buffer (1× NEB DNA/RNA Protection Reagent, 1% (w/v) polyvinylpyrrolidone-40 ...
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD-SNAP was biotinylated or fluorescently labelled by overnight incubation at 4°C with SNAP-Biotin® or SNAP-Surface® Alex Fluor® 647 (New England BioLabs) with at least a 3:1 molar excess of these labels to PfK5ΔL6-MD-SNAP ...
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bioRxiv - Pathology 2021Quote: RT-qPCR was also performed on RNA extracted from the 4 dpi lung samples with NEB Luna Universal One-Step RT-qPCR Kit with SYBR-Green (NEB, Ipswich, MA, USA) to quantify the cytokines IL-6 and IFN-β ...