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Citations for New England Biolabs :
5201 - 5250 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 1× BSA (New England Biolabs), 1 mM dNTPs (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of exonuclease (NEB) was added to each reaction and incubated for 15 minutes at 37 °C followed by 15 minutes at 80 °C and the samples were purified using 1x AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Biophysics 2023Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
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bioRxiv - Systems Biology 2023Quote: 1 µL of DpnI (NEB) was added to the plate with PCR products and incubated at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... ERK (9102, NEB, 1:10000); phospo-ERK (9101 ...
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bioRxiv - Neuroscience 2023Quote: ... AKT (9272, NEB, 1:5000); Phospo-AKT (9271 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM dNTPs (NEB - N0446S), 1.2 units SUPERaseIN Rnase Inhibitor (Thermo Fisher – AM2696) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 U T4 ligase (NEB) and the fragment 1 & 2 for each mutant ...
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bioRxiv - Immunology 2023Quote: ... 1 µL Phusion polymerase (NEB), and remainder water (to 50 µL) ...
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bioRxiv - Neuroscience 2023Quote: SOX10 (89356, NEB, 1:400), sox10 zebrafish (AB229331 ...
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bioRxiv - Neuroscience 2023Quote: ... p21 (2946, NEB, 1:3000), Isl1/2 (67.4E12 ...
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bioRxiv - Cell Biology 2023Quote: ... Cy3-(1:400, Jackson Biolabs), Alexa 546-(1:400 ...
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bioRxiv - Immunology 2023Quote: ... 1 mM ATP (NEB, # P0756S), 1 µl Esp3l (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 uL of DpnI (NEB) was added directly to the PCR reactions for 1 hr at 37°C ...
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bioRxiv - Physiology 2024Quote: ... with 1x GlycoBuffer 1 (NEB).
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bioRxiv - Synthetic Biology 2024Quote: ... 1 mM dNTPs (NEB #N0447L), 250 nM of the first primer (IDT ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 mM dNTPs (NEB #N0447L), 250 nM of the first primer (IDT ...
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bioRxiv - Microbiology 2024Quote: ... 1 x adenylation buffer (NEB), and 1 mM ATP ...
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bioRxiv - Physiology 2024Quote: ... with 1x GlycoBuffer 1 (NEB), 5 μL of PNGaseF (500 U/μL ...
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bioRxiv - Physiology 2024Quote: ... with 1x GlycoBuffer 1 (NEB). Following incubation ...
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bioRxiv - Biophysics 2024Quote: ... A 1 kbp ladder (NEB) was used for the relative comparison of the bands.
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bioRxiv - Evolutionary Biology 2024Quote: ... 1 µL factor mix (NEB), 250 nM MS2-tagged 50S ribosomal subunit ...
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bioRxiv - Plant Biology 2024Quote: ... when OD600 reached 0.5 for 4 h at 37 °C and then they were purified using amylose resin column (New England Biolabs, for MBP related proteins purification) or Ni-NTA agarose column (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... Final concentrations of 5 µM TDP-43-TEV-MBP and the indicated final concentrations of recombinant proSAAS or BSA (NEB BioLabs; B9001S) were achieved by mixing aliquots of a 44 μM stock solution of TDP-43-TEV-MBP with aliquots of stock solutions of either 55 μM proSAAS or 151 μM BSA (both in 5 mM acetic acid) ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acid (5 μL) was used as template for reverse transcription using LunaScript® RT SuperMix Kit (New England Biolabs, Hitchin, UK) in 20 μL reaction volume ...
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bioRxiv - Molecular Biology 2022Quote: ... cell lysates were adjusted to the same protein concentration using furin cleavage buffer (100 mM Tris-HCL and 1mM CaCl2) and incubated with 5 units of Furin (NEB, Ipswich, MA) for 16 h at 30℃ ...
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bioRxiv - Microbiology 2022Quote: ... colony PCR was conducted to amplify the 5’ IGR of the major T6 cluster using OneTaq DNA Polymerase (New England Biolabs – MA, USA). PCR products were confirmed with gel electrophoresis and Sanger sequencing by Eton Bioscience Inc ...
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bioRxiv - Plant Biology 2021Quote: ... 500 ng of the SAP-treated RNA was treated with 12.5 units RNA 5’ Pyrophophohydrolase (RppH; New England BioLabs; Ipswitch, MA, USA) in 1X T4 RNA Ligase Buffer (New England BioLabs ...
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bioRxiv - Cell Biology 2022Quote: ... and transformed into competent DH5a E. coli (5-alpha Competent E. coli) following the protocol provided by the company (New England Biolabs, Ipswich, MA). For colony selection ...
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bioRxiv - Molecular Biology 2020Quote: Thermocycler was used to denature and anneal 5 µl of PCR product as recommended by manufacturer (EnGen™ Mutation Detection Kit, NEB, E3321S). Thermocycler was adjusted for heteroduplex formation as following ...
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bioRxiv - Plant Biology 2020Quote: ... The suitability of the selected restriction enzyme pair was confirmed by digesting 400 ng of genomic DNA using 5 Units of each restriction enzyme and NEB CutSmart™ buffer (10×) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2021Quote: ... 1 uL of the Gibson Assembly reaction mixture was transformed via heat shock into chemically competent NEB 5-alpha cells (NEB, Catalog #C2988J), and cells were incubated on lysogeny broth with 10 μg/mL tetracycline (LB-Tet10 ...
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bioRxiv - Microbiology 2021Quote: ... and total of 5 μg of the five fragments was ligated in an equal molar ratio by T4 DNA ligase (New England Biolabs, Ipswich, MA) at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: Vector pFLD was digested with PmlI at 37°C for 2 h and dephosphorylated with 5 U Antarctic phosphatase at 37 °C for 30 min as recommended by the manufacturer (NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... 39 μl of each lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1-2 μl (400-800 units ...
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bioRxiv - Bioengineering 2022Quote: Reunion of 4-6 nM of dsDNA template (gblocks® Gene Fragment, IDT) to a solution of 5 U/μL of T7 RNA polymerase (NEB, M0251), 200 nM Cas9 (S ...
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bioRxiv - Biochemistry 2022Quote: ... and the impurities that were tagged by Chitin Binding Domain were extracted on a gravity-flow column using 5 mL Chitin resin (New England BioLabs, Evry, France). The target proteins were purified with HisTrap HP columns (GE Life Sciences ...
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bioRxiv - Genomics 2022Quote: ... we amplified a 150 bp sequence using primers pGL3-CDC20_F and pGL3-CDC20_R (Phusion High-Fidelity PCR Master Mix, NEB M0531, Supplemental Table 5) that added restriction sites for SacI and XhoI to the 150 bp sequence ...
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bioRxiv - Microbiology 2024Quote: ... 39 μl of each benzonase-treated lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1 μl (400 units ...
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bioRxiv - Evolutionary Biology 2023Quote: ... before being incubated in 5% normal goat serum dissolved in PBS-TX (NGS-PBS-TX; NGS; New England BioLabs, Hitchin, Hertfordshire, UK) for a minimum of one hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and amplified with the primers (5’-CAA GAC TAG TGG AAG CGG AGC TAC TAA CTT CAG CCT GCT GAA GCA GGC TGG CGA CGT GGA GGA and 5’-NNN NAC GCG TCT AGC CTT CCC AGA CGT ACC C) using high-fidelity Phusion polymerase (NEB, Cat# M0530S). The PCR fragment was digested with BmtI and MluI ...
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bioRxiv - Molecular Biology 2023Quote: In vitro transcribed RNAs were treated with Antarctic phosphatase (Fermentas, Waltham, MA) to remove the 5’ terminal phosphate and then labeled by T4 polynucleotide kinase (New England Biolabs, Ipswitch, MA) in the presence of γ-32P-labeled ATP (PerkinElmer ...
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bioRxiv - Synthetic Biology 2023Quote: ... The resulting fragment was subjected to error-prone PCR under the following conditions: 5 U of Taq DNA polymerase (New England Biolabs, MA, USA), 200 μM of each deoxynucleoside triphosphate ...
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bioRxiv - Synthetic Biology 2024Quote: ... The amplicons were purified and subjected to error-prone PCR under the following conditions: 5 U Taq DNA polymerase (New England Biolabs, MA, USA), 200 μM of each deoxynucleoside triphosphate ...
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bioRxiv - Microbiology 2024Quote: ... primers (forward primer 5’gcacccgggATGAGTGATACTAAAGAGACTGTTCC3’ and reverse primer 5’gacctcgagTTAACAACATCCTCCTTTCTTTT3’) were used in PCR amplification using Phusion® High-Fidelity PCR master mix (M0531S, New England Biolabs, USA). The amplified products were electrophoresed on 1% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... Decapping of the 5’ DTB-GTP cap was performed to leave the 5’ monophosphate for ligation by adding 20 μl of capped RNA to 2.2 μl of 10X ThermoPol buffer (NEB product number B9004) and 2 μl RppH (NEB product number M0356S) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 x concentrated Phusion HF buffer and 1 U Phusion DNA Polymerase (New England Biolabs). The PCR reaction was performed as follows ...
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bioRxiv - Biochemistry 2021Quote: ... 1 ng/μl of template DNA and 1 U of Phusion polymerase (New England Biolabs). The mutant PCR fragments were then cloned into pSpurMS2 (a gift from Dr ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...