Labshake search
Citations for Illumina :
3101 - 3150 of 3793 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... These filters were chosen to keep the types of samples selected from SRA uniform with the samples generated in this study (i.e., paired-end RNA-seq reads generated on an Illumina platform). The 1.5 Gbp threshold was chosen as it is roughly half the minimum number of bases (∼3.2 Gbp ...
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bioRxiv - Biochemistry 2023Quote: ... using 100 bp paired-end reads at a depth of ∼30 million reads/sample and concurrently run with the PhiX quality control library (Illumina).
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bioRxiv - Neuroscience 2022Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cDNA amplification and library preparation (10X v3 chemistry) were performed for samples according to manufacturer protocol and sequenced on a NovaSeq S4 (Illumina) to a depth of >30,000 reads/cell.
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bioRxiv - Immunology 2023Quote: ... Library preparation (10X v2 chemistry) was performed for samples according to manufacturer protocol and sequenced on a NovaSeq S4 (Illumina) to a depth of >25,000 paired reads/cell.
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bioRxiv - Immunology 2023Quote: ... cDNA amplification and library preparation (10X v3 chemistry) were performed for samples according to manufacturer protocol and sequenced on a NovaSeq S4 (Illumina) to a depth of >30,000 reads/cell.
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bioRxiv - Immunology 2023Quote: ... cDNA amplification and library preparation (10X v3 chemistry) were performed for samples according to manufacturer protocol and sequenced on a NovaSeq S4 (Illumina) to a depth of >30,000 reads/cell.
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bioRxiv - Cancer Biology 2023Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Paired-end sequencing libraries were prepared from amplified cDNA with the Nextera XT DNA sample Prep Kit (Illumina, #FC-131) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA library preparation with oligo-dT primers following the Illumina TruSeq stranded mRNA sample preparation guide (Illumina, San Diego, CA), and sequencing procedure with Illumina HiSeq 3000 platform with 30-50x106 bp reading depth were previously described in detail 19 ...
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bioRxiv - Bioengineering 2023Quote: RNA was extracted from the spinal cord and prepared for RNA sequencing with TruSeq Standard mRNA sample Prep Kit (Illumina). Deep sequencing was performed on the Illumina NovaSeq6000 ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA isolated from murine mammary adipose tissue contralateral to the tumor was used to prepare sequencing libraries with Illumina TruSeq Stranded Total RNA Sample Preparation kit and sequenced using an Illumina HiSeq 2000 instrument (Illumina).
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bioRxiv - Genomics 2024Quote: All samples were processed using the 10x Genomics Chromium 3’ Single Cell Protocol and sequenced using NovaSeq 6000 S1 (Illumina). For the first sample containing NSC pools 1.1 and 1.2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were prepared with the TruSeq Stranded Total RNA LT or TruSeq Stranded Total RNA-seq Sample Prep Kit from Illumina and sequenced paired-end for 6-hour and 72-hr ...
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bioRxiv - Physiology 2024Quote: ... and sequenced (75 nucleotides, single end) with a median depth of 25 million reads per sample (NextSeq 550, Illumina, USA).
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bioRxiv - Microbiology 2024Quote: ... isolated RNA samples were deprived of ribosomal RNA with Illumina Ribo-Zero Plus rRNA Depletion Kit (Illumina, San Diego, CA) and processed into a cDNA library for Illumina with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was confirmed to be of high quality using an Agilent Tapestation using an RNA screentape (RIN >9.8 for these samples) and 500ng was prepared for RNA-seq using Truseq RNA library preparation protocol with poly-A capture (Illumina). Final libraries were quantified using an Agilent Tapestation using a D1000 screentape and sequenced using an Illumina NextSeq2000 to generate libraries with 64 – 78 million paired-end 65bp reads.
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bioRxiv - Neuroscience 2024Quote: ... and IDT adapters for Illumina TruSeq DNA UD Indexes (96 Indexes, 96 Samples) for total RNA or using the TruSeq Small RNA Library Prep (Illumina) for miRNAs ...
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bioRxiv - Molecular Biology 2024Quote: ... was performed at 7-14 million reads per sample in single-ended mode with 100 cycles on the NextSeq 2000 platform (Illumina). Demultiplexed FASTQ files were generated with bcl-convert v4.3.6 (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: ... was performed at 13-16 million reads per sample in single-end mode with 100 cycles on the NextSeq 2000 platform (Illumina). Demultiplexed FASTQ files were generated with bcl-convert v4.0.3 (Illumina) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and mate-pair libraries of 3-kb insert size were prepared using the Nextera Mate Pair Sample Preparation Kit (cat. No. FC-132-1001, Illumina). We performed DNA shearing for the fragment and mate-pair library preparations using Covaris S220 ...
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bioRxiv - Microbiology 2024Quote: ... Enriched samples were then normalised and libraries were prepared using the Illumina DNA prep kit (Illumina Inc., San Diego, USA) using 1/4 of the recommended volume ...
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bioRxiv - Microbiology 2024Quote: A total of 11,887 samples were identified from the publicly available CRyPTIC dataset5 that (i) had been whole genome sequenced using short-read (Illumina) approaches and (ii ...
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bioRxiv - Genomics 2024Quote: ... The RNA-seq libraries were created according to the manufacturer’s instructions using the Tru-Seq RNA sample prep kit (Illumina, Inc.). In brief ...
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bioRxiv - Immunology 2024Quote: ... and library preparation was performed according to the TruSeq Stranded mRNA Sample Preparation protocol using the TruSeq Stranded mRNA HT Kit (Illumina). Sequencing was performed using an Illumina NovaSeq 6000 with a 2 50-bp read length at the Finnish Functional Genomics Centre at the University of Turku and Åbo Akademi as well as Biocenter Finland.
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bioRxiv - Genomics 2024Quote: ... Sample replicates were subjected to library preparation with 12 cycles of amplification (NEBNext Ultra II DNA Library Prep Kit, Illumina). Libraries were sequenced on Illumina HiSeq (50 cycles ...
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bioRxiv - Genomics 2024Quote: ... using Illumina’s TruSeq Stranded mRNA HT sample prep kit (Catalog #20020595) utilizing poly-A selection of mRNA following the protocol outlined by Illumina in their user guide ...
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bioRxiv - Cell Biology 2024Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using SR Cluster Kit cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... An Illumina sequencing library was then created from the fragmented and rRNA-depleted RNA using the TruSeq Stranded Total RNA HT sample prep kit (Illumina) following the protocol and with 10 cycles of PCR for library amplification ...
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bioRxiv - Microbiology 2024Quote: ... was performed at a minimum of 7 million reads per sample in single-ended mode with 100 cycles on the NextSeq 2000 platform (Illumina). Demultiplexed FASTQ files were generated with bcl- convert v4.2.4 (Illumina) ...
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bioRxiv - Immunology 2024Quote: ... Targeted sequencing was performed on FFPE tumor samples from all patients using the NovaSeq platform (Illumina, San Diego, CA, USA). The library for sequencing was constructed using the lymphoma-associated gene mutation detection kit provided by Nanjing Shihejiyin Technology (Nanjing ...
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bioRxiv - Immunology 2024Quote: ... Samples were sequenced at 27 – 132 million reads per sample with 50 bp read length using NovaSeq 6000 sequencing system with NovaSeq SP flow cell (Illumina). Raw data analysis and alignment to Zhang et al ...
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bioRxiv - Molecular Biology 2024Quote: ... After library construction samples were quality controlled again and were sequenced paired end (PE) 28/91 on a NovaSeq 6000 system (Illumina). We aimed to reach >30’000 reads per nucleus for all samples ...
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bioRxiv - Neuroscience 2024Quote: ... was performed at 21 to 41 million reads/sample in single-end mode with 100 nt read length on the NextSeq 2000 platform (Illumina). Demultiplexed FASTQ files were generated with bcl2fastq2 v2.20.0.422 or bcl-convert v4.0.3 (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μg of genomic DNA per patient sample was sequenced using the Illumina Whole Genome Sequencing Service with the Illumina HiSeq 2000 (Illumina), generating 200 bp (2 × 100 bp reads ...
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bioRxiv - Microbiology 2024Quote: ... Plate-based RNA sample prep was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Illumina Ribo-Zero rRNA Removal Kit (Bacteria) and the TruSeq Stranded Total RNA HT sample prep kit following the protocol outlined by Illumina with the following conditions ...
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bioRxiv - Neuroscience 2024Quote: ... was performed at ∼25 million reads/sample in single-end mode with 75 nt read length on the NextSeq 500 platform (Illumina). Demultiplexed FASTQ files were generated with bcl2fastq2 v2.20.0.422 (Illumina).
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bioRxiv - Animal Behavior and Cognition 2024Quote: We sent 12 total RNA samples to Novogene for cDNA library prep (polyA enrichment) and paired-end 150 bp sequencing (NovaSeq PE150 on NovaSeq6000, Illumina). The samples consisted of 3 males and females from each season ...
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bioRxiv - Molecular Biology 2024Quote: ... Newborn and maternal samples were sequenced on separate flow cells (NextSeq 500/550 High Output Kit v2.5 75 Cycles, Illumina, 20024906) using 1600 pg/sample in the newborn cohort and ...
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bioRxiv - Immunology 2024Quote: Libraries for each sample were sequenced on a NextSeq550 75 Cycle High Output sequencing kit (Illumina Inc., Sunnyvale, CA, USA). For each library ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: RNA-sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA Sample Preparation Kit (Cat#20020594, Illumina, San Diego, CA). RNA was extracted from isolated cardiomyocytes using the QIAGEN RNeasy Fibrous Tissue Mini Kit (Cat#74704 ...
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bioRxiv - Immunology 2024Quote: ... The multiplexed samples and Phi X 174 library were prepared via standard protocols and sequenced on a MiSeq instrument (Illumina).
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bioRxiv - Microbiology 2024Quote: ... Most bacterial and phage samples were sequenced using single or paired-end sequencing on the Illumina Nextseq platform (Illumina, Inc). ICP3 mutants were sequenced using Plasmidsaurus (Plasmidsauraus ...
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bioRxiv - Pathology 2024Quote: ... Approximately 2 µg of RNA per sample was sent to the Yale Genomics Core for sequencing using the Illumina TruSeq Stranded mRNA kit (Illumina).
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bioRxiv - Plant Biology 2024Quote: ... After clustering of the index-coded samples using the cBot Cluster Generation System (TruSeq PE Cluster Kit v3-cBot-HS, Illumina), the libraries were sequenced on the Illumina Hiseq PE150 platform ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries from each sample were pooled and run using paired-end (300 million read pairs per sample) sequencing on the NovaSeq 6000 platform (Illumina) at Novogene (Cambridge).
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bioRxiv - Microbiology 2024Quote: ... The RNA was dephosphorylated in 3’ and then phosphorylated in 5’ to generate cDNA libraries using the NebNext Small RNA Sample Prep kit with 3’ sRNA Adapter (Illumina) according to the manufacturer’s protocol with 12 cycles of PCR amplification in the last step followed by DNA purification with Monarch PCR DNA cleanup kit (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... The index-coded samples were clustered on a cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer ‘ s guidelines ...
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bioRxiv - Physiology 2024Quote: ... an equal quantity of each sample library) and clustered at a concentration of 20 pmol on paired-end sequencing flow cell CBU2FANXX (Illumina). Sequencing is performed for 2 x 125 cycles on a HiSeq 2500 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... The samples were pooled in equimolar concentrations and sequenced (2 x 150 bp, PE) on a Novaseq platform (Illumina, USA). All kits were used as per the manufacturer’s instructions with minor modifications.