Labshake search
Citations for Illumina :
8751 - 8781 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... All of the subsequent steps were performed by the CRG Genomics Unit (Barcelona): sequencing libraries were prepared from the pure high molecular weight DNA using TruSeq DNA HT Library Preparation kit (Illumina ® HiSeq ® Sequencing v4 Chemistry). A paired-end library with a target insert size of ∼500 bp was sequenced on an Illumina® HiSeq2500 platform in paired-end mode ...
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bioRxiv - Developmental Biology 2021Quote: ... We assessed the quality of RNA using fragment analysis to obtain RNA integrity number (RIN) before proceeding with Library preparation (NEBNext® Ultra™ RNA Library Prep Kit, Illumina, San Diego, CA, USA). Each sample was sequenced in Illumina HiSeq 2000 (Single read ...
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bioRxiv - Microbiology 2022Quote: The 16S and ITS libraries were prepared by the sequencing facility using the Illumina TruSeq≡Nano DNA Library Prep Kit (Illumina, Inc., San Diego, CA, United States), llumina MiSeq was run using a v2 Standard sequencing format with paired end reads (2 x 250 bp) ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA quality control was performed in a fragment analyzer and sequencing libraries were constructed using a Nextera DNA Flex kit (now renamed to Illumina DNA Prep, Illumina, San Diego, CA) and 2×150 BP paired-end sequencing was performed in an Illumina NextSeq500 ...
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bioRxiv - Genomics 2020Quote: A total of 720 metagenomic libraries were prepared using Illumina-specific Nextera DNA transposase adapters and a Tagment DNA Enzyme and Buffer Kit (Illumina, San Diego, CA, cat. no. 20034197) (modified from Baym et al ...
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bioRxiv - Systems Biology 2020Quote: ... Supernatants were discarded and nuclei were re-suspended in 50 μl reaction buffer (2.5 μl of Tn5 transposase and 25 μl of TD buffer from a Nextera DNA Library Prep Kit from Illumina, and 22.5 μl nuclease-free H2O). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... A second PCR was performed to attach dual indices and Illumina® sequencing adapters using the Nextera XT Index kit (Illumina®; San Diego, CA, USA). The conditions of this PCR included ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1 μl of each Nextera XT Index Kit v2 set B or set C barcoding primer (Illumina, Cat# FC-131-2002 or FC-131-2003). These reactions were then incubated at 95°C for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... WGS libraries were sequenced with paired-end 150 base pair reads on an Illumina NovaSeq 6000 by the UW Biotechnology Center (Illumina NovaSeq 6000 S1 Reagent Kit v1.5).
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for each sample were prepared from ≥200 ng of sheared DNA with TruSeq® Nano DNA High Throughput Library Prep Kit (20015965, Illumina Inc., San Diego, CA, USA) and IDT for Illumina – TruSeq® DNA UD Indexes v1 (Illumina Inc. ...
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bioRxiv - Genomics 2023Quote: ... we processed 500 ng of total RNA using the TruSeq Stranded mRNA Library Prep kit (Illumina, Cat # 20020595; Indexes: IDT for Illumina TruSeq RNA UD Indexes, cat # 20020591). The resulting libraries were then analyzed on a TapeStation (Agilent ...
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bioRxiv - Plant Biology 2023Quote: ... RNA libraries were prepared from samples that met the Illumina TruSeq® Stranded Total RNA Sample Preparation Guide (15031048 E) input guidelines using the Illumina TruSeq® Stranded Total (Plant) RNA Sample Preparation kit (Illumina Inc., San Diego, California, USA). For each library preparation ...
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bioRxiv - Molecular Biology 2024Quote: Multiplexed ChIP-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Preparation kit (E7103S) with 25-50 ng of input or IP DNA and barcode adaptors (NEBNext Multiplex Oligos for Illumina [Set 1, E7335] & [Set 2, E7500]). 150-nucelotides (150- nt ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing libraries were generated with Illumina Stranded mRNA Prep kit and sequencing of 2×50 bp paired-end reads was performed on an Illumina NovaSeq 6000 (Illumina Inc., San Diego, CA, United States). The sequences were aligned to the human GRCh38 reference genome using STAR (58) ...
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bioRxiv - Systems Biology 2024Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit and then sequenced on the Illumina MiSeq platform (Illumina, San Diego, CA, USA) at the Kunming Institute of Botany ...
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bioRxiv - Evolutionary Biology 2024Quote: ... to ensure sufficient RNA of high quality in each sample and prepared libraries using the Illumina TruSeq® mRNA Stranded Kit (Product#: RS-122-2101, Illumina, Inc., 5200 Illumina Way, San Diego, CA 92122). We then used the Qubit Fluorometer to quantify the amount of cDNA in each sample prior to sequencing.
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bioRxiv - Genomics 2022Quote: ... Libraries for Illumina sequencing were generated with NEBNext® Ultra™ RNA Library Prep Kit for Illumina and sequenced on the Illumina NovaSeq6000 (Illumina, Inc., San Diego, CA, USA). Libraries for long-read sequencing were generated with the cDNA PCR sequencing kit (SQK-PCS109 ...
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bioRxiv - Molecular Biology 2023Quote: ... and sequenced on an Illumina NextSeq 500 system using the NextSeq 500/550 Mid Output Kit v2.5 (#20024904, Illumina; 150 cycles, paired-end run 2x 75 bp) with an average of 1×107 reads per RNA sample.
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bioRxiv - Cancer Biology 2023Quote: ... Triplicates were pooled for a target total cell number of 60,000 in 50uL to load less than 20uL into the 10X controller or perform TDE1-based tagmentation reactions on small volumes (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit). The 10X Genomics Chromium Platform was used to generate a targeted 5,000 single cell Gel Bead-In-Emulsions (GEMs ...
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bioRxiv - Cancer Biology 2023Quote: ... The library preparation for bulk tumors and cell lines was generated using the TruSeq Stranded mRNA sample preparation kit (Illumina Inc., San Diego, RS-122-2101/2) according to the manufacturer’s instructions (Illumina ...
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bioRxiv - Genomics 2024Quote: ... bidentatus DNA extraction and library preparation was performed by Dovetail Genomics (Scotts Valley, CA, USA) with the TruSeq DNA PCR-Free kit (Trueq Index 5, Illumina, San Diego, CA, USA, mean size 475bp). 150 bp paired-end sequencing was performed on one lane of HiSeqX by Novogene (Cambridge ...
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bioRxiv - Immunology 2024Quote: ... 500 ng of total RNA was used for polyA selection and Truseq library preparation according to the instructions provided by Illumina (TruSeq RNA Sample Prep Kit v.2), with 8 cycles of PCR ...
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bioRxiv - Developmental Biology 2024Quote: ... we prepared n=40 sequencing libraries from 1µg total RNA per sample using Illumina® Stranded mRNA Prep kits with unique 5′ and 3′ index adapter pairs (IDT for Illumina® RNA UD Indexes, Set A). Libraries were PCR amplified for 10 cycles ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 600 ng of the amplified DNA was pooled and employed to build a single paired end (2 × 150 bp) genomic library using the NEBNext® UltraTM II DNA Library Prep Kit by Illumina (New England Biolabs, Ipswich, MA, USA) (Asem et al. ...
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bioRxiv - Microbiology 2020Quote: ... Samples that met the Illumina sample input guidelines were prepared according the TruSeq® Stranded mRNA Sample Preparation Guide (Rev. E) using the Illumina® TruSeq® Stranded mRNA Sample Preparation kit (Illumina Inc., San Diego, California, USA). For each library preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR-amplified cDNA was purified using AMPure XP beads and processed with the Nextera XT DNA Library Preparation Kits (Illumina, Cat. # FC-131-1024 and FC-131-1096) as per the protocol provided by the manufacturer.
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bioRxiv - Genetics 2020Quote: ... Libraries were made according to the manufacturer’s directions for the TruSeq Stranded mRNA LT Sample Prep Kit – sets A and B (Illumina, Cat. No. RS-122-2101 and RS-122-2102). Resulting short fragment libraries were checked for quality and quantity using the Bioanalyzer (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... and sequenced on an Illumina NovaSeq 6000 system using the NovaSeq 6000 S4 Individual Lane Loading Reagent kit (#20028313, Illumina; 150 cycles, paired end run 1x 111 bp) with an average of 2×107 reads per sample ...
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bioRxiv - Cancer Biology 2023Quote: ... ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (Illumina, cat# RS-122-2301 and #RS-122-2302), and transcriptome capture based libraries with the TruSeq RNA Access Library Prep Kit (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... ribosomal RNA depleted strand-specific RNA libraries with the TruSeq Stranded Total RNA LT sample preparation kit with Ribo-Zero Gold (Illumina, cat# RS-122-2301 and #RS-122-2302), and transcriptome capture based libraries with the TruSeq RNA Access Library Prep Kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... was performed on an Illumina NextSeq machine using either a 300 cycle v2 mid output or high output kit (Illumina, Code FC-404-2003 or Code FC-404-2004) under standard Illumina® procedure as previously described[9].