Labshake search
Citations for Illumina :
8651 - 8700 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 100-500 ng of total RNA with RIN values of 9.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with eight cycles of PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole-genome sequences were obtained (MiSeq Desktop Sequencer, 2×250 v2 paired-end chemistry and the Nextera XT DNA Library Preparation Kit, Illumina, San Diego, California, US) per manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... RNA-seq libraries were prepared from 1 μg of total RNA using the Illumina TruSeq RNA Library Prep Kit (Illumina, San Diego, CA, USA) and sequenced on an Illumina NovaSeq 6000 platform (2 × 150 bp ...
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bioRxiv - Microbiology 2024Quote: ... The library was then diluted to a concentration of 15 pM and sequenced with a 15% PhiX spike-in on an Illumina MiSeq sequencing platform using the MiSeq Reagent Kit v3 (600 cycles) (Illumina, Inc., San Diego, CA). The resulting read lengths were 301 bp (forward sequences) ...
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bioRxiv - Molecular Biology 2024Quote: ... 800 ng of RNA was used as input for library preparation using the TruSeq Stranded Total RNA Library Prep Gold kit (Illumina, San Diego, CA, USA) with rRNA Removal Mix ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing was carried out on NextSeq 500 sequencing system with NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles) chemistry (Illumina, Inc. USA, Cat. No. 20024905).
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bioRxiv - Microbiology 2024Quote: ... NGS was carried out on the NextSeq 500 sequencing system with NextSeq 500/550 Mid Output Kit v2.5 (300 Cycles) chemistry (Illumina, Inc. USA, Cat. No. 20024905). Applied read length was in the 150-300 bp range ...
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bioRxiv - Microbiology 2024Quote: Paired-end libraries (2×151 bp) were prepared for a subset of samples using Illumina TruSeq DNA library preparation kit (Illumina, San Diego, CA, USA) and sequenced on the Illumina NextSeq500 platform ...
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bioRxiv - Microbiology 2024Quote: ... Illumina sequencing libraries were generated by PCR amplification using specific adaptors (NovoNGS CUT&Tag 4.0 High-Sensitivity Kit for Illumina B box, Novoprotein, Suzhou, China). The CUT&Tag libraries were sequenced using the Illumina Novaseq 6000 platform (Beijing Novogene Technology Co. ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation for sequencing experiments was performed for Figure 1 using the Illumina TruSeq ChIP-Seq Library Preparation kit (Illumina, San Diego, CA, USA) and for Figure 2 using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... and the pooled libraries were sequenced on an Illumina MiSeq system using the MiSeq Reagent Kit v3 with 600 cycles (Illumina, San Diego, CA, USA). All raw datasets were denoised by correcting the amplicon errors and used to infer the exact amplicon sequence variants (ASVs ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA of all samples was used to prepare paired-end libraries with the Nextera ™ DNA FlexLibrary Preparation kit according to the manufacturer’s instructions (Illumina Inc., San Diego, CA). The Library pool of the samples was sequenced using the S4 flow cell of Illumina NextSeq 550 instrument in a paired-end fashion (read length 151 bp ...
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bioRxiv - Cancer Biology 2024Quote: ... Transposition was performed directly on nuclei using 25 μl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina Tagment DNA kit #20034210). Tagged DNA was subjected to PCR amplification and library indexing ...
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bioRxiv - Microbiology 2024Quote: ... DNA paired-end libraries (2 × 250-bp) were constructed with 1 ng of the viral genome as input using the Nextera XT DNA kit (Illumina, Inc., San Diego, USA) and sequenced on the Illumina MiSeq for 39-hours ...
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bioRxiv - Systems Biology 2024Quote: ... quantification kit and sequenced at the University of Washington Northwest Genomics Center with the Illumina NextSeq 500 High Output v2 kit (Illumina Inc., San Diego, CA). The sequencing run generated an average of 75 million base-pair paired-end raw read counts per library ...
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bioRxiv - Molecular Biology 2024Quote: ... and sequenced on the Illumina NovaSeq 6000 sequencer with the S1-200 reagent kit to generate approximately 60 million 100- base read pairs per sample (Illumina, Inc., San Diego, CA). Read adapters were detected using BBMerge (v37.02 ...
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bioRxiv - Genomics 2024Quote: ... and the libraries were sequenced (2×75nt) on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Genomics 2024Quote: ... Paired-end sequencing (2×150nt) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 300 cycles (Illumina #FC-420-1004, discontinued).
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bioRxiv - Immunology 2024Quote: ... 0.33ng of each sample was used to construct a pool of uniquely identified samples with the Nextera XT kit (Illumina Cat# FC-131-1096) and a second amplification of the pooled samples was conducted with 12 cycles ...
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bioRxiv - Plant Biology 2024Quote: ... and the DNA was purified before conversion into a sequencing library (NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7645)) using Illumina-compatible adaptors (NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... 100-500 ng of total RNA with RIN values of 9.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with 8 cycles of PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA obtained from each sample was subjected to a sequencing library construction using the TruSeq Stranded mRNA Library Prep Kit (Illumina, Inc., San Diego, CA) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2024Quote: ... coli isolates was performed using the Illumina NovaSeq platform following the construction of sequencing libraries using the Illumina DNA Prep kit (Illumina, San Diego, CA, USA). The quality of the resulting paired-reads was assessed using FASTQC [24] ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted with commercial kits after mechanical cell disruption and metagenomic sequencing was performed on a NovaSeq6000 or HiSeq4000 Illumina sequencer (Illumina, San Diego, CA, USA) in order to produce 100 M of ∼150 bp paired-end reads per sample ...
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bioRxiv - Microbiology 2024Quote: ... The libraries were sequenced on an Illumina MiSeq with 2 × 300 paired-end cycle protocol using the MiSeq Reagent Kit v3 (600-cycle) (Illumina, cat# MS-102-3003).
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries with an insert size of approximately 600 bp were prepared using Nextera DNA Flex library preparation kits (Illumina, San Francisco, United States), with the addition of Illumina PhiX DNA ...
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bioRxiv - Genomics 2024Quote: ... RNA samples with a RIN value ≥ 8 were used to prepare sequencing libraries with the TruSeq Stranded mRNA Kit (Illumina, San Diego, CA, USA) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... 200 ng of cDNA was used as input for library preparation using Illumina Nextera XT DNA library preparation kit (Illumina, Inc., San Diego, CA) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: cDNA libraries for RNA-Seq were constructed from the extracted RNA samples using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) following the procedures outlined in the Reference Guide ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were sequenced on an Illumina Miseq or Nextseq using various reagent kits for paired end reads (MS-103-1002 Illumina, MS-102-2003 Illumina). All libraries were prepared in house and sequenced in the Genomics Research Unit at Tel-Aviv University (Israel) ...
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bioRxiv - Genetics 2024Quote: ... A second round of PCR was performed using indexed primer sets to construct sequencing libraries using a Nextra XT Index Kit (Illumina, San Diego, CA, USA). The final libraries were purified using Ampure XP beads and sequenced on an Illumina MiSeq system in 150 × 150 paired-end format ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and a total of 4 μg of total RNA was used to construct paired-end libraries using the TruSeq RNA Sample Preparation Kit v3 (Illumina, Inc., San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... This work benefited from the facilities and expertise of the I2BC High-throughput Sequencing Platform (Gif-sur-Yvette, Université Paris-Saclay, France) for oriented library preparation (Illumina Truseq RNA Sample Preparation Kit) and sequencing (Paired-end 75 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... Library preparation was as previously described and library sequencing performed using the Illumina MiSeq platform with the 2 × 300v3 Reagent Kit (Illumina Inc., San Diego, CA, USA) (Avramenko et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... library preparation and sequencing: Synthesis of cDNA was performed using NEBNext Ultra Directional RNA Library Prep Kit from Illumina (New England Biolabs; cat. no. E7420) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... After A-tailing and adaptor ligation fragments that carry adaptors on both ends were enriched by 8 cycles of PCR (Illumina TruSeq Nano DNA Sample Kit). The final library was quantified using PicoGreen (Quant-iT ...
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bioRxiv - Microbiology 2020Quote: ... A paired-end sequencing library (2×250bp) was created using a VAHTS Universal DNA Library Prep kit for Illumina® (Illumina, San Diego, CA, USA) and sequenced within an Illumina HiSeq system (Illumina Inc.).
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bioRxiv - Immunology 2021Quote: ... Libraries were prepared following 10X Genomics protocols (Chromium Single Cell 3’ Reagent Kits v2 Chemistry) and sequenced on NovaSeq 6000 (Illumina S2 flow cell, paired-end). FASTQ files were processed using cellranger (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Approximately 5 ug of total RNAs were used to construct cDNA libraries (NEBNext Ultra Directional RNA Library Prep Kit for Illumina, Illumina, San Diego, CA, USA). Transcriptome sequencing was performed using the Illumina Hi-Seq 2000 platform ...
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bioRxiv - Genomics 2020Quote: ... transcripts were purified from total RNA using oligo(dT) magnetic beads and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS122-2102). A D1000 Screen Tape assay (Agilent ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We prepared a genomic DNA library of these 343 DNA samples using a modified version of the Nextera DNA library prep kit (∼ 350bp insert size, Illumina Inc., San Diego, CA, USA) meant to conserve reagents ...
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bioRxiv - Cancer Biology 2020Quote: ... A sequencing library was constructed using 100 ng of total RNA and a TruSeq RNA Access Library Prep Kit (Illumina, Inc., San Diego, CA, USA), according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... before fragmenting the samples to 450bp fragments and processing with the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA). Over the three-year duration of the sequencing phase of the project ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was extracted from the discs and libraries for Illumina HiSeq2000 sequencing were generated using the TruSeq RNA Sample Preparation Kit (Illumina, catalog ID RS-122-2002). Reads were mapped using Bowtie2 v ...
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bioRxiv - Cell Biology 2021Quote: ... transcripts were purified from total RNA using oligo(dT) magnetic beads and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS122-2102) (9).
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bioRxiv - Microbiology 2021Quote: ... Total RNA of the paddy soils was subjected to an rRNA removal procedure using the Ribo-Zero rRNA removal bacteria kit (Illumina Inc., San Diego, CA, USA) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: Total RNA purified from hippocampi of WT and Atf6b−/− mice (n=2 per group) was used to prepare RNA libraries using a TruSeq Stranded mRNA Sample Preparation Kit (Illumina, Inc., San Diego, CA, USA), with polyA selection for ribosomal RNA depletion ...
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bioRxiv - Cancer Biology 2020Quote: ... The 3-cell control sample was sequenced on an Illumina MiSeq using a v2 300-cycle kit in 2×150 bp paired-end mode (Illumina, cat. no. MS-102-2002). For the patient samples ...
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bioRxiv - Genetics 2022Quote: ... then pooled them and sequenced the 2×75 bp paired-end libraries on one Flowcell of an Illumina NextSeq 550 with a High Output v2 kit (150 cycles) (Illumina Inc., San Diego, California, USA).
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bioRxiv - Microbiology 2022Quote: ... 5 ng of total RNA was used for intact RNA library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760, New England BioLabs). To obtain sufficient RNA for the analysis of oral samples ...