Labshake search
Citations for Illumina :
8501 - 8550 of 8781 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: A cDNA library was prepared from RNA extracted from bat sarbecoviral isolates using the TruSeq Stranded Total RNA LT Sample Prep Kit Gold (Illumina, San Diego, CA, USA) for Rc-mk2 and Rc-kw8 strains or the MGIEasy RNA Directional Library Prep kit (MGI ...
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bioRxiv - Genomics 2022Quote: ... Barcoded libraries were pooled in equimolar amounts and sequenced as 2×300mer reads on an Illumina MiSeq platform using a MiSeq Reagent Kit v3 (Illumina, Cat#: MS-102-3003). 50% PhiX Control v3 (Illumina ...
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bioRxiv - Immunology 2022Quote: ... For cohort I, RNA-seq libraries were generated by GenomeScan (Leiden, the Netherlands) with the TruSeq RNAseq RNA Library Prep Kit (Illumina Inc., Ipswich, MA, USA), and were sequenced using Illumina HiSeq 4000 generating ~20 million 150 bp paired end reads for each sample ...
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bioRxiv - Microbiology 2022Quote: ... Genome sequencing was performed by the Microbial Systems Molecular Biology Laboratory at the University of Michigan using an Illumina Nextera™ sequencing kit and a MiSeq (Illumina, San Diego, CA).
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bioRxiv - Genetics 2020Quote: ... and ligated to index-containing sequence adaptors using the Illumina TruSeq Stranded mRNA Sample Preparation Kit (Cat. #RS-122-2101, Illumina, Inc., San Diego, CA). The molar concentrations of the indexed libraries were measured using the 2100 Agilent Bioanalyzer (Agilent Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... was used to perform automated preparation of strand-specific RNA-seq libraries using the TruSeq Stranded Total RNA with Ribo-Zero Gold kit (cat# RS-122-2393, Illumina, San Diego, CA, USA). According to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: ... 1µg total RNA was used for library preparation with the TruSeq Stranded mRNA Library Prep Kit High Throughput (Cat# RS-122-2103, Illumina, San Diego, CA, USA). Libraries were quality-checked on the Fragment Analyzer (Advanced Analytical ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg of total uterine RNA was sent to the NIH Intramural Sequencing Center to create a libarary using the TruSeq RNA Kit (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: The V3-V4 hypervariable regions of the 16S_rDNA were amplified by two steps PCR using v1 primers (Vaiomer) and sequenced using MiSeq Reagent Kit v3 (2×300 bp Paired-End Reads, Illumina, San Diego, CA, USA) as previously described [24] ...
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bioRxiv - Microbiology 2021Quote: Paired-end libraries were constructed with 0.2 ng/μl of purified DNA using the Nextera XT DNA Library Prep Kit as recommended by the manufacturer (Illumina, San Diego, CA, USA) and were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Shotgun metagenomic libraries were then constructed from a subset of these animals (LDC N = 15, DDC N = 7, CZMD N = 7) using the Nextera XT kit (Illumina, San Diego, CA USA) and sequenced on and Illumina HiSeq 3000 using a 150bp PE sequencing kit (Illumina).
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bioRxiv - Microbiology 2020Quote: ... the PCR amplicons were pooled and subjected to library preparation for Next Generation Sequencing using the Nextera XT library prep kit (Illumina, San Diego, CA, USA). The library was normalized and sequenced using a MiSeq nano v2 2×250 sequencing kit ...
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bioRxiv - Microbiology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina, San Diego, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Shotgun metagenomics and isolates genome sequencing was performed on the NextSeq 500 using NextSeq High Output v2 Kit (300 Cycles) (Illumina, San Diego, CA, USA) by the Sequencing Core Facility at The Novo Nordisk Foundation Center for Biosustainability (Technical University of Denmark ...
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bioRxiv - Microbiology 2021Quote: ... The trimmed DNA fragment sequences were then used to prepare a shotgun paired-end library with an average insert size of 350 bp using a TruSeq DNA Sample Prep kit (Illumina, San Diego, CA, USA). The library was sequenced on an Illumina MiSeq platform (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we used 2 μg total RNA for library preparation following the protocol in the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). The samples were sequenced by single-end reads (75 bp ...
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bioRxiv - Genetics 2021Quote: ... The thirty-seven sequencing libraries were created by reverse-transcription from 2 µg of RNA from each sample using the TruSeq Stranded mRNA Library Prep kit (Illumina, San Diego, CA, USA). Index adapters were added to identify sequences for each sample in the final data ...
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bioRxiv - Genomics 2021Quote: ... 2 × 150 bp reads for each parent were generated on an Illumina NovaSeq 6000 instrument using the NovaSeq S2 reagent kit (Illumina, San Diego, California, USA). A total of 539,570,835 reads for the N ...
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bioRxiv - Genomics 2021Quote: ... and 2 × 150 bp reads were produced on an Illumina NovaSeq 6000 instrument using the NovaSeq S2 reagent kit (Illumina, San Diego, California, USA). An average of 57,521,215 reads were generated for each of the 4 samples ...
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bioRxiv - Genomics 2021Quote: ... libraries with an average insert size of 450 bp were prepared by the University of Utah’s Huntsman Cancer Institute High-Throughput Genomics Core Facility using the Illumina TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, California, USA). 2 × 150 bp reads for each parent were generated on an Illumina NovaSeq 6000 instrument using the NovaSeq S2 reagent kit (Illumina ...
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bioRxiv - Genomics 2021Quote: ... We then added adapters and barcodes onto the ends of the fragmented DNA to generate indexed sequencing libraries using TruSeq Library Preparation Kit (Illumina, San Diego, CA, U.S.). We performed capture-based target enrichment using the SeqCap EZ Hybridization and Wash Kit (Roche NimbleGen ...
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bioRxiv - Microbiology 2021Quote: ... and the pooled DNA was used for the preparation of sequencing libraries using the Nextera XT DNA library prep kit (Illumina, San Diego, CA, USA) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RNA libraries from all samples were then prepared for shotgun transcriptomic sequencing using the TruSeq Stranded Total RNA Library kit from Illumina (San Diego, CA, USA), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... 100-500 ng of total RNA with RIN values of 9.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with 8 cycles of PCR ...
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bioRxiv - Genomics 2021Quote: ... For long read sequencing (Oxford Nanopore Technologies) total RNA was extracted as described above and depleted using the Ribo-Zero rRNA Removal Kit Plant Leaves (Illumina, San Diego, CA, USA). RNA was then purified and concentrated on a RNA Clean Concentrator™-5 column (Zymo Research ...
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bioRxiv - Genomics 2021Quote: Six Illumina RNA-Seq libraries (one for each organ) were constructed from 500ng total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows for mRNA strand orientation (the orientation of sequences relative to the antisense strand is recorded) ...
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bioRxiv - Cancer Biology 2020Quote: ... Sequencing was performed using the NextSeq 500/550 High-Output v2 (150 cycle) Kit (# FC-404-2002) on the NextSeq 550 platform (Illumina Inc, #SY-415-1002). Libraries were combined in an equimolar pool based on the library quantification results and run across 5 High-Output Flow Cell v2.5.
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bioRxiv - Developmental Biology 2021Quote: ... and sequencing libraries of poly(A)+ RNAs were then generated using the VAHTSTM mRNA-seq v2 Library Prep Kit from Illumina (Vazyme Biotech, NR601-01) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Dual barcode indices and sequencing adaptors were attached to each amplicon using the Illumina Nextera XT Index kit (Illumina, Inc., San Diego, CA, USA) following manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Illumina sequencing was carried out via amplification of the V4 hypervariable region of the 16S rRNA gene with the Earth Microbiome modified F515/R806 primers (22) on a MiSeq platform using a MiSeq 600 cycle kit (Illumina, San Diego, CA, USA). Analysis of the V4 regions was carried out using QIIME2 (21) ...
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bioRxiv - Genomics 2020Quote: ... BS-converted DNA (100 ng) of each sample was used for preparing the BS-seq library via the TruSeq DNA Methylation Kit (Illumina, EGMK91324, San Diego, CA). The quality of the libraries was checked using Qubit assays (Q32850 ...
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bioRxiv - Immunology 2020Quote: ... was adjusted to 1 μg and used for poly (A) mRNA enrichment and stranded specific RNA-Seq library preparation with TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA). Quality control of prepared libraries were performed using Agilent Bioanalyzer DNA 1000 kit and Qubit DNA HS fluorescence kit ...
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bioRxiv - Microbiology 2020Quote: ... Five DNA extractions were performed per soil sample and pooled for metagenomic library preparation with the TrueSeq DNA kit (Illumina Inc., San Diego, CA). Sequencing was performed on the Illumina HiSeq platform to generate 6.4 billion of paired-end reads of 150bp.
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bioRxiv - Immunology 2021Quote: ... The V(D)J enriched library was then constructed via Chromium Single Cell 5’ Library Construction Kit (10x Genomics Cat#1000020) and libraries were sequenced in a NovaSeq™ 6000 Sequencing System (Illumina, San Diego, CA). V(D)J sequences were collapsed using Cell Ranger ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries for WGS were prepared using Nextera XT DNA Library Prep Kit and sequencing was performed on the Illumina NextSeq500 machine as per manufacturer’s protocol (Illumina Inc., San Diego, California, USA) producing 2 x 151 base pair reads.
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bioRxiv - Cancer Biology 2020Quote: ... Strand specific RNA-seq libraries were constructed using the Illumina TruSeq Stranded Total RNA Library preparation Gold kit (20020598, Illumina Inc., San Diego, CA) as per the instructions ...
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bioRxiv - Immunology 2021Quote: ... Library preparation was done using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with poly-A selection (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Library preparation for sequencing was performed using 200 ngs of total RNA input with the TrueSeq RNA Sample Prep Kit v2-Set B (RS-122-2002, Illumina Inc, San Diego, CA) producing a 275bp Fragment including Adapters in average size ...
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bioRxiv - Physiology 2020Quote: ... 200ng of RNA per sample were used as the input of TruSeq Stranded mRNA Library Prep kit to make barcoded libraries according to the manufacturer’s protocol (Illumina Inc., San Diego, CA, USA). The library concentration was determined by qPCR using the Library Quantification Kit Illumina Platforms (Kapa Biosystems ...
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bioRxiv - Genomics 2021Quote: ... Paired-end sequencing libraries were prepared using the TruSeq Nano DNA Library Prep kit following the manufacturer’s specifications (Illumina Inc., San Diego, CA, USA). Out of the 150 libraries ...
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bioRxiv - Genomics 2021Quote: ... Paired-end libraries were constructed from the double stranded cDNA by using Illumina DNA Prep with Enrichment kit (Illumina Inc., San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Paired-end sequencing libraries were constructed with an insert size of 500 bp using a TruSeq DNA PCR-Free kit (Illumina, San Diego, CA, USA) according to Illumina library preparation protocols ...
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bioRxiv - Genomics 2020Quote: ... and 8 to 11 kb were generated using the gel selection-based protocol of the Nextera mate pair kit (Illumina, San Diego, CA, USA) and a 0.6% agarose gel in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the amplicons were purified and sequenced on a MiSeq flow cell using Reagent Kit v3 and 2×300 pair-end reads (Illumina Inc., San Diego, USA) according to the manufacturer’s instructions at the Göttingen Genomics Laboratory (G2L ...
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bioRxiv - Cancer Biology 2021Quote: ... sequencing libraries using total RNA generated from frozen tissues with a TruSeq mRNA Library Preparation Kit using poly-A-enriched RNA (Illumina, San Diego, CA, USA) and enriched with the human kinome DNA capture baits (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Illumina MiSeq sequencing was performed at the Genome Institute of Singapore according to the MiSeq reagent kit v2 (2 × 250 bp) preparation guide (Illumina, San Diego, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... and sequenced on the Illumina NextSeq 2000 sequencer with the P3-200 cycle reagent kit in order to generate approximately 50M million 100-base read pairs per sample (Illumina, Inc., San Diego, CA).
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were pooled and sequenced single-end (1×75) on an Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA) at a sequencing depth of 23-31 million reads ...
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bioRxiv - Cell Biology 2022Quote: ... an average of 500 pg of amplified cDNA was used to prepare the libraries following Nextera XT DNA kit (Illumina Ref. FC-131-1096). Libraries were multiplexed by 6 on a high-output flow cells ...
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bioRxiv - Cell Biology 2022Quote: ... as described in the section “Quantitative real-time PCR.” The library was prepared using the TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions and sequenced on an Illumina NovaSeq 6000 platform in the 101 bp single-end mode ...