Labshake search
Citations for Illumina :
5551 - 5600 of 5691 citations for TD 5108 CAS 866933 46 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Selected samples from MPX virus-positive patients were directly sequenced without prior PCR amplification by the Illumina technology with the Nextera XT paired-end strategy on a MiSeq instrument (Illumina Inc., San Diego, CA, USA) or ...
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bioRxiv - Evolutionary Biology 2022Quote: ... USA) for library preparation and 150 base pair (bp) paired-end whole-genome sequencing on an Illumina NovaSeq 6000 (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Cell Biology 2022Quote: The libraries were generated using depleted RNA obtained from 1 μg of total RNA by TruSeq Sample Preparation RNA Kit (Illumina, Inc., San Diego, CA, USA) according to the manufacturer’s protocol without further modifications ...
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bioRxiv - Microbiology 2024Quote: ... and was accomplished by parallel long read sequencing with Oxford Nanopore (Oxford Nanopore technologies, Oxford, UK) and short read sequencing with Illumina (Illumina Inc., San Diego, CA, USA). DNA for Oxford Nanopore was prepared using the Nanopore Ligation Sequencing kit (SQK-LSK109 ...
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bioRxiv - Neuroscience 2024Quote: ... Purified total RNA was used to generate mRNA libraries using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep kit for Illumina (Illumina Inc., San Diego, CA, USA). Quality and quantity were assessed using an Agilent DNA1000 series chip assay and Invitrogen Qubit HS Kit (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... All cDNA libraries were generated from 1 µg total RNA using the TrueSeq Stranded Total RNA Library Preparation Kit with the Ribo-Zero Gold Kit according to the manufacturer’ ss instructions (Illumina Inc., San Diego, CA, USA). Quality and quantity of the cDNA libraries were assessed using a TapeStation 4200 (D1000 screen tape assay ...
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bioRxiv - Genomics 2024Quote: ... were used to prepare Illumina libraries using the Nextera DNA Flex Library Prep Kit according to the manufacturers’ instructions (Illumina Inc. San Diego, CA, USA). Library quality was measured via electrophoresis in D1000 ScreenTape on an Agilent 4200 TapeStation System (Agilent Technologies Netherlands BV ...
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bioRxiv - Microbiology 2024Quote: ... and controls (n=30) were amplified using PCR with the F515 and R806 primer pair and sequenced using Illumina MiSeq platform (Illumina Inc., San Diego, CA, USA) (82) ...
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bioRxiv - Cell Biology 2023Quote: ... Purified total RNA was used to generate mRNA libraries using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep kit for Illumina (Illumina Inc., San Diego, CA, USA). Quality and quantity were assessed using an Agilent DNA1000 series chip assay and Invitrogen Qubit HS Kit (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... Libraries were prepared using the TruSeq® RNA Sample Prep v2 kit with the IDT for Illumina DNA/RNA UD indexes (Illumina, San Diego, CA, USA). Purification and fragmentation of mRNA ...
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bioRxiv - Genomics 2023Quote: ... libraries were prepared from liver tissues using an Illumina TruSeq Nano DNA Library Prep Kit and paired-end (PE150) sequenced on the Illumina Novaseq 6000 sequencing platform (Illumina, Inc., San Diego, CA, USA). We used gonad tissues for ONT PromethION library preparation and sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation for VLP genomic DNA involved using >25ng input DNA with the Nextera DNA Flex Library Preparation Kit (currently named Illumina DNA prep, Illumina, CA, USA). Deviating from the Illumina protocol’s full reaction volume (50 µL) ...
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bioRxiv - Cell Biology 2023Quote: cDNA libraries were prepared from 1 μg total RNA of each sample using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and R2-Uni13 (5’-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GAG TAG AAA CAA GG-3’) (adaptor and barcode oligonucleotide sequences from Illumina, Inc., San Diego, CA, USA). Annealing and extension steps were performed for 30 s at 55oC and 7 m at 72oC ...
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bioRxiv - Genetics 2022Quote: ... markers used for genetic heterozygosity evaluation was carried out according to manufacturer-recommended standard protocols on a custom-designed Illumina Infinium XT microarray (Illumina, Inc., San Diego, CA, USA). Protocols for microarray design ...
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bioRxiv - Microbiology 2023Quote: ... A genomic library with average insert size of 350 bp was prepared for sequencing using a TruSeq Nano DNA library preparation kit (Illumina, Inc., San Diego, CA, USA). Paired-end 2 x 125 nt sequencing was performed by the MGX platform (CNRS ...
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bioRxiv - Microbiology 2023Quote: ... The 16S rRNA gene V4-V5 region was amplified from each water sample and sequenced using the Illumina MiSeq platform (Illumina Inc., San Diego, CA, USA) (Tremblay et al. ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was fragmented and simultaneously tagged so that the peak size of the fragments was approximately 500 bp using the Nextera® transposome (Illumina Inc., San Diego, CA). After purification of the transposome using DNA Clean & ConcentratorTM-5 (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... Parallel aliquots of the DNA samples used for ONT sequencing were used to prepare Illumina libraries using the Nextera DNA Flex Library Prep Kit as per protocol (Illumina Inc., San Diego, CA, USA). Library quality was measured using an Agilent 4200 TapeStation System as described above ...
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bioRxiv - Genomics 2024Quote: ... The libraries were prepared with Ampliseq Library Plus for Illumina kit (Illumina, San Diego, CA, USA and using Illumina’s Ampliseq CD indexes set A(Illumina), and their quality was assessed with High Sensitivity DNA reagents on an Agilent 2100 Bioanalyzer System ...
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bioRxiv - Plant Biology 2024Quote: ... The sequencing of the libraries was conducted on Illumina NovaSeq instrument with 150 bp paired-end reads on NovaSeq S1 flowcell (Illumina Inc., San Diego, CA, USA) at the Centre of Plant Structural and Functional Genomics in Olomouc ...
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bioRxiv - Genomics 2024Quote: ... For each pool 16 GB (1 GB per sample) of 150 bp paired-end sequencing was performed on the Illumina NovaSeq 6000 platform (Illumina Inc., San Diego, CA, USA) by BaseClear B.V ...
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bioRxiv - Pathology 2024Quote: ... A detailed description of the database and how it is maintained has been published elsewhere.47 BioVU participant DNA samples were genotyped on a custom Illumina Multi-Ethnic Genotyping Array (MEGA-ex; Illumina Inc., San Diego, CA, USA). Quality control included excluding samples or variants with missingness rates above 2% ...
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bioRxiv - Microbiology 2024Quote: ... The samples were diluted to obtain a concentration of 100–500 ng and used for library preparation with the Illumina DNA Prep kit (Illumina Inc., San Diego, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and library sequencing was performed using either the HiSeq4000 (single-end, 50bp) or the NovaSeq6000 (single-end, 100 bp) Illumina (Illumina, Inc., San Diego, CA, USA) platform ...
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bioRxiv - Microbiology 2024Quote: ... cDNA libraries were multiplexed and clustered in one lane of a flow cell for sequencing on a MiSeq® System (Illumina, San Diego, CA, U.S.A.) with 2 × 300 bp paired-end (PE ...
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bioRxiv - Microbiology 2024Quote: ... The different libraries were pooled according to the effective concentration and the target amount of data – 6 Gb raw data per sample – and were sequenced by the Illumina NovaSeq 6000 (Illumina Inc., San Diego, CA, USA). Paired-end 150bp reads were generated.
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was fragmented and simultaneously tagged so that the peak size of the fragments was approximately 500 bp using the Nextera® transposome (Illumina Inc., San Diego, CA). After purification of the transposome by DNA Clean & ConcentratorTM-5 (Zymo Research ...
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bioRxiv - Genomics 2024Quote: ... DNA extracted from venous blood underwent whole-genome sequencing using the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA) and Illumina HiSeq 2000 or HiSeq X sequencer ...
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bioRxiv - Cancer Biology 2024Quote: ... The generated barcoded libraries containing fragments of approximately 300 bp (paired-end) were subjected to sequencing on the Illumina® NovaSeq™ 6000 platform (Illumina, San Diego, CA). An average of 52.2 million paired-end reads were generated for each sample (Table S4) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the libraries were pooled equimolarly and 150 bp paired-end sequencing was performed by Novogene (Cambridge, UK) on the Illumina NovaSeq 6000 (Illumina Inc., San Diego, CA, USA) platform.
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bioRxiv - Neuroscience 2022Quote: ... The concentration was adjusted to 2 nM prior to sequencing (50 bp) on a HiSeq 2000 sequencer (Illumina, Inc., San Diego, USA) using TruSeq SR Cluster kit v3-cBot-HS (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... using a Pipin prep (Sage Science, Beverly, MA, USA) and sequenced using S1 chemistry (paired-end, 2×50 bp sequencing) on a Novaseq sequencer (Illumina, Cambridge, UK). 2 biological replicates were analysed per condition.
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bioRxiv - Microbiology 2022Quote: ... 15 Gb output) was used according to the manufacturer’s instructions for library preparation and sequencing on a MiSeq 2 (Illumina, San Diego, USA). The quality of the obtained sequences was checked with FastQ files generated at the end of the run (MiSeq Reporter Software ...
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bioRxiv - Genomics 2021Quote: ... and the other smaller cohort for replicating the effect size of methylation sex differences (AEMS450K2, Athero-Express Methylation Study Illumina 450K 2). RNA-sequencing was performed on plaques for which DNA methylation data was available (420 out of 485 plaques) ...
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bioRxiv - Microbiology 2020Quote: ... and submitted for DNA sequencing using the MiSeq Reagent kit v3 with the 2 × 300 bp paired-end sequencing protocol (Illumina Inc., USA).
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bioRxiv - Cancer Biology 2020Quote: ... Sequencing was performed at The Centre for Applied Genomics (Toronto, Canada) across 2 lanes of an Illumina HiSeq2500 rapid run flowcell (Illumina, San Diego) with SR100 bp.
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bioRxiv - Immunology 2022Quote: ... Paired-end next-generation sequencing was performed on the Illumina MiSeq system 500 (2 x250 bp) (Illumina, San Diego, California, United States). TCR sequencing analysis was performed using RTCR as previously described(46).
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bioRxiv - Immunology 2021Quote: ... commercial reagents were used for unique dual indexed amplicon library generation according to the Artic protocol (NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®)) ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s instructions and sequenced using the Illumina MiSeq platform with a read length of 2 × 150 bp (Illumina, San Diego, USA). In total ...
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bioRxiv - Cancer Biology 2021Quote: Strand-specific libraries were generated using the TruSeq Stranded mRNA samples preparation kit (illumine Inc., San Diego, RS-122-2101/2) according to manufacturer’s instructions (Illumina, part #15031047 Rev.E). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... libraries were prepared with Nextera XT DNA Library Preparation Kit69 and paired-end (2×150 bp) sequencing was performed on a NextSeq 500 machine (Illumina Inc., USA) at the George Washington University Genomics Core facility ...
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bioRxiv - Plant Biology 2020Quote: RNA sequencing libraries of time course JA-treated root samples were made using 2 μg of total RNA following TruSeq® Stranded mRNA Library Preparation protocol (Illumina, USA). Equal amount libraries were pooled and sequenced using Illumina Novoseq platform ...
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bioRxiv - Genomics 2020Quote: ... 2 µl each of P7 and P5 Nextera XT Index Kit v2 index primers (Illumina Catalogue numbers FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Cancer Biology 2021Quote: ... and PCR and sequencing were performed as described previously (2 x 250bp paired-end reads, on an Illumina Miseq (Lemont, IL, USA)) [68–70] ...
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bioRxiv - Cancer Biology 2021Quote: ... Strand-specific libraries were generated using the TruSeq Stranded mRNA samples preparation kit (illumine Inc., San Diego, RS-122-2101/2) according to manufacturer’s instructions (Illumina, part #15031047 Rev.E). Briefly ...
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bioRxiv - Genomics 2020Quote: ... Up to 96 AOIs were pooled and sequenced on a NextSeq High Output v2.5 (75 cycles, 2×38 bp; Illumina, cat. no. 20024906).
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bioRxiv - Microbiology 2023Quote: ... and finally sequenced with phiX as control using paired-end 2×250-bp chemistry on the Illumina MiSeq 2000 platform (Illumina Inc., USA)
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bioRxiv - Microbiology 2023Quote: ... The quantified and denatured libraries were sequenced using paired-end 2×250-bp chemistry with 5% PhiX as an internal control on the Illumina MiSeq 2000 platform (Illumina Inc., USA). The quality-filtered reads were de-novo assembled using SPAdes (version 3.11.1) ...
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bioRxiv - Microbiology 2023Quote: ... The prepared library was sequenced using a MiSeq sequencing system with a V3 reagent kit (300 × 2 bp; Illumina, 4–6 samples). Lake Biwa viral contigs/genomes (LBVs ...