Labshake search
Citations for Illumina :
5351 - 5400 of 5691 citations for TD 5108 CAS 866933 46 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Between 3-5 µg of total RNA from each sample was used for mRNA enrichment with the RiboZero Bacterial rRNA Removal Kit (Illumina, San Diego, CA, USA), and the quality and quantity of enriched mRNA was assessed as described above for total RNA ...
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bioRxiv - Microbiology 2023Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... The DNA libraries were then prepared at the Institut Pasteur Mutualized Platform for Microbiology (P2M) with the Nextera XT kit (Illumina, San Diego, CA, USA) and sequencing was performed with the NextSeq 500 system (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... Poly-A selected strand specific library construction and paired-end sequencing (2x 125 bp on a HiSeq 2500 using v4 chemistry; Illumina, San Diego, CA, USA) was performed at the VBCF NGS Unit (Vienna ...
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bioRxiv - Immunology 2022Quote: ... variable regions V3 to V4 of the bacterial 16S ribosomal RNA gene were amplified by limited cycle PCR and subjected to the Illumina 16S metagenomic sequencing library preparation workflow according to the manufacturer’s recommendations (Illumina Inc., San Diego, CA, USA). The obtained DNA libraries were quantified ...
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bioRxiv - Cancer Biology 2022Quote: ... and sequenced on the Illumina NextSeq 2000 sequencer with the P3-200 cycle reagent kit in order to generate approximately 50M million 100-base read pairs per sample (Illumina, Inc., San Diego, CA).
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were pooled and sequenced single-end (1×75) on an Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA) at a sequencing depth of 23-31 million reads ...
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bioRxiv - Cell Biology 2022Quote: ... as described in the section “Quantitative real-time PCR.” The library was prepared using the TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions and sequenced on an Illumina NovaSeq 6000 platform in the 101 bp single-end mode ...
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bioRxiv - Genomics 2022Quote: ... Short-read sequencing data for error correction was generated from the same individual using the TruSeq Nano DNA Low Throughput Library Prep Kit and IDT for Illumina – TruSeq DNA UD Indexes (Illumina, San Diego, CA, USA). Standard protocols were followed to yield a paired library with a 350 bp insert size and 50 bp standard deviation ...
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bioRxiv - Genomics 2022Quote: ... A total of 100 ng of RNA was used as input to the mRNA sequencing library prep kit (TruSeq RNA Access Library Prep Kit by Illumina, San Diego, CA, USA) according to the protocols of the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... The concentration of amplified cDNA was measured with Qubit and 1 ng was used as input for Nextera XT (Illumina, San Diego, CA, USA) library preparation using New P5 SMART PCR oligo and Nextera index oligos ...
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bioRxiv - Genetics 2022Quote: ... were captured by xGen Exome Research Panel v2 (Integrated DNA Technologies, Coralville, Iowa, USA) and sequenced with Novaseq 6000 (Illumina, San Diego, CA, USA) as 150 bp paired-end reads ...
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bioRxiv - Genomics 2022Quote: ... the sequencing was performed with paired-end sequencing of 150nt each end on one lane of Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA) per sample ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was used for cDNA synthesis and purification using a low-throughput protocol with a TruSeq Stranded RNA LT Kit (Illumina, San Diego, CA, USA). A half-scale reaction of the standard protocol was used for library preparation ...
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bioRxiv - Plant Biology 2022Quote: Pair-end library preparation for MiSeq sequencing was conducted using the two-step tailed PCR method described on Illumina (Illumina, San Diego, CA, USA). We used the following primer pairs to amplify partial sequences of the 16S rRNA gene:
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bioRxiv - Microbiology 2022Quote: ... with up to 100 ng DNA input following the manufacturers protocol using internal dual-index 8 bp barcodes with Nextera® adapters (Illumina, San Diego, CA). All libraries were quantified with TapeStation®(Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... A total of 21 isolates were selected (one representative from each cluster) for whole-genome sequencing using the Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA) platform ...
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bioRxiv - Microbiology 2022Quote: ... These programs cover “Tagment Genomic DNA” to “Amplify DNA” in the protocol (Mantis-Nextera DNA Flex library prep protocol, Illumina, San Diego, CA, USA) and “Clean Up Libraries” in the protocol (Epmotion - Library Clean Up protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared from purified RNA (∼1 μg) using Illumina TruSeq standard mRNA library preparation kit as per manufacturer protocol (Illumina Inc., San Diego, CA). Briefly ...
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bioRxiv - Physiology 2022Quote: ... and used to construct stranded RNA-seq libraries from polyA-selected total liver RNA using an Illumina stranded TruSeq mRNA Prep Kit (Illumina, San Diego, CA, USA). Three pooled RNA samples were prepared for each of the four treatment groups ...
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were fragmented and tagged with the indexed adaptors using the Nextera DNA XT Sample Preparation Kit (Illumina, San Diego, CA, USA). The sequence library was quantified by using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... All samples showed a high RNA quality (RNQ>8) and were sequenced using an Illumina® HiSeq 3000 sequencer (Illumina, San Diego, CA, USA). Reads were mapped to the Sscrofa11.1 assembly of the Swine Genome Sequencing Consortium (SGSC ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were pooled and sequenced (single-end 75 bp reads) on Illumina NextSeq 500 using High Output V2 Kit (Illumina, San Diego, CA, USA). Sequencing data were analyzed as described previously (70 ...
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bioRxiv - Genomics 2022Quote: ... 500 ng per sample of isolated DNA was bisulfite converted and hybridized with the Infinium Mouse Methylation BeadChip (Illumina Inc., San Diego, CA, USA), from 24 C57BL/6 mice (all female ...
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bioRxiv - Microbiology 2022Quote: ... Amplicons of the V3-V4 region of the 16S rRNA gene and the and the ITS1 region and library prep were generated by Herculase II Fusion DNA Polymerase Nextera XT Index Kit V2 (Illumina, San Diego, CA, USA). The products of the library prep were validated by an Agilent Tapestation D1000 (Santa Clara ...
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bioRxiv - Microbiology 2022Quote: ... Indexed amplicons were pooled in equimolar amounts and sequenced using Illumina’s v3 600-cycle (paired-end) reagent kit on an Illumina MiSeq benchtop sequencer (Illumina Inc., San Diego, CA, USA) after all DNA extraction blanks and PCR reagent blanks were confirmed for negative amplification.
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bioRxiv - Immunology 2022Quote: ... sequencing was performed by the Biomedical Sequencing Core Facility of the Center for Molecular Medicine (Center for Molecular Medicine, Vienna, Austria) on an Illumina HiSeq 3000/4000 (Illumina, San Diego, CA, USA). For donor 1 ...
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bioRxiv - Microbiology 2022Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... A second short-run PCR was performed in order to ligate the Illumina i7 and i5 barcodes (Nextera XT, Illumina, San Diego, CA, USA), and amplicons were purified again as above ...
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bioRxiv - Plant Biology 2022Quote: ... The GBS library was diluted to 3.6 pM and sequenced on one lane (single end, 101 base pair read length) of an Illumina HiSeq 2500 (Illumina Inc, San Diego, CA) at the Genomics Resources Core Facility (Weill Cornell Medicine ...
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bioRxiv - Neuroscience 2022Quote: ... Sequencing libraries were prepared by transposase-assisted tagmentation and enrichment of 3’end of genes using Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA, USA). The libraries were sequenced on the HiSeq2500 for 25 and 75 cycles paired-end reads ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing was performed by the NIDDK Genomics Core facility using a MiSeq system with the single-end 50 bp Sequencing Kit (Illumina, San Diego, CA, USA). Sequence reads were mapped to the reference genome (NC_000913.3 ...
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bioRxiv - Plant Biology 2022Quote: ... Illumina sequencing pair-end libraries with an insert size of 300 bp were prepared using the Nextera DNA Flex Library Prep Kit (Illumina, San Diego, CA, USA). After the libraries were constructed ...
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bioRxiv - Microbiology 2023Quote: ... The final pooled library was submitted for high throughput sequencing on the Illumina MiSeq platform using the PE250 V2 chemistry method (Illumina, San Diego, CA, USA). For this work ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg total RNA was used for preparation of cDNA libraries with the TruSeq Small RNA Library Preparation Kit (Illumina, San Diego, CA, USA). Libraries were sequenced as 50 bp paired-end reads on an Illumina Genome Analyzer ...
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bioRxiv - Immunology 2023Quote: ... Libraries were pooled and single end sequenced (1X75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA).
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bioRxiv - Cell Biology 2022Quote: ... 75bp) on a NextSeq500 using the NextSeq 500/550 High Output Kit v2 according to the manufacturer’s instructions (Illumina Inc., San Diego, CA, USA). Analysis of RNA-sequence reads ...
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bioRxiv - Evolutionary Biology 2022Quote: ... sequencing library with a peak insert size of 535 bp was constructed using 200 ng of genomic DNA with a TruSeq Nano LT library preparation kit (Cat # FC-121-4002, Illumina, San Diego, CA, USA) according to the kit supplier’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library with a peak span size of 1450 bp and a mean span size of 1027 bp was prepared from 570 ng of genomic DNA by tagmentation using an MP library preparation kit (Cat # FC-132-1001, Illumina, San Diego, CA, USA), without size selection ...
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bioRxiv - Systems Biology 2023Quote: ... The libraries were quantitated by qPCR and sequenced on one lane for 101 cycles from one end of the fragments on a HiSeq 4000 (Illumina, San Diego, CA, USA). Fastq files with 100 bp reads were generated and demultiplexed with the bcl2fastq v2.17.1.14 Conversion Software (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A MIG-seq library was prepared following the protocol suggested by the development team [42] and sequenced utilizing the MiSeq system (Illumina, San Diego, CA, USA) and MiSeq Reagent Kit v3 (150 cycle) ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... purified input RNAs and immunoprecipitated RNA fragments were sent for library construction with the VAHTS Total RNA-Seq (H/M/R) Library Prep Kit for Illumina (Vazyme, Nanjing, China) and sequencing using the Illumina HiSeq 2500 (Illumina, San Diego, CA, USA). Quality control and raw sequencing data filtration were achieved with the FastQC and fastp software ...
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bioRxiv - Genomics 2023Quote: ... by the University of Wisconsin-Madison Biotechnology Center (Madison, WI, USA) as specified in the TruSeq DNA PCR-Free Reference Guide (Illumina, San Diego, CA, USA) [17] ...
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bioRxiv - Microbiology 2023Quote: ... The microbial community of each sample was analyzed by 16S rRNA gene amplicon sequencing of the V4-V5 hypervariable region (Illumina MiSeq platform) (Illumina Inc., San Diego, CA) using 515F and 806R universal primers at the MrDNA Laboratory (Molecular Research LP ...
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bioRxiv - Physiology 2024Quote: ... The 16S rRNA V4 gene amplicon sequencing was performed with a V2-500 cycle cartridge on the MiSeq platiorm (Illumina, San Diego, CA, USA). The raw fastq files were processed with the DADA2 pipeline 1.16 (R package ...
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bioRxiv - Physiology 2024Quote: ... were used to construct individual Poly-A enriched cDNA libraries by Novogene (Beijing, China) using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the manufacture’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... 500 ng of the extracted total RNA was used for library construction using the TruSeq RNA Sample Preparation Kit V2 (Illumina, San Diego, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The library cDNA was subjected to paired-end sequencing with a pair end 125-base pair reading length on an Illumina HiSeq 2500 sequencer (Illumina, San Diego, CA, USA). Gene expression data are included in Supplemental Data 1.
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bioRxiv - Genomics 2024Quote: ... It consisted of 13 trios from the endangered Mexican Wolf (MW, Canis lupus baileyi) population genotyped using the Illumina CanineHD BeadChip array (Illumina, Inc., San Diego, CA). The MW population was reintroduced into the wild in the 1990s ...
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bioRxiv - Microbiology 2024Quote: ... Pair-end libraries were generated using a Nextera XT DNA Kit and sequenced using a NovaSeq 6000 system (both from Illumina, San Diego, CA, USA). Sequenced data were preprocessed using Trimmomatic v.0.33 and FastQC v.0.12.1 ...