Labshake search
Citations for Illumina :
5251 - 5300 of 5691 citations for TD 5108 CAS 866933 46 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Library preparation for sequencing was performed using 200 ngs of total RNA input with the TrueSeq RNA Sample Prep Kit v2-Set B (RS-122-2002, Illumina Inc, San Diego, CA) producing a 275bp Fragment including Adapters in average size ...
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bioRxiv - Physiology 2020Quote: ... 200ng of RNA per sample were used as the input of TruSeq Stranded mRNA Library Prep kit to make barcoded libraries according to the manufacturer’s protocol (Illumina Inc., San Diego, CA, USA). The library concentration was determined by qPCR using the Library Quantification Kit Illumina Platforms (Kapa Biosystems ...
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bioRxiv - Genomics 2021Quote: ... and subsequently sequenced in paired-end mode with 150- bp read length on a NextSeq500 to obtain ∼40 mio reads (Illumina, San Diego, CA, USA).
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bioRxiv - Genomics 2021Quote: ... we had genomic DNA from the cell line HCT116 profiled (Princess Margaret Genomics Centre, Toronto, ON, Canada) using the Infinium MethylationEPIC BeadChip array (Illumina, San Diego, CA, USA). We prepared these samples as technical replicates of the HCT116 genomic DNA that we later spiked with 0.01 ng spike-in control ...
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bioRxiv - Genomics 2021Quote: ... Paired-end sequencing libraries were prepared using the TruSeq Nano DNA Library Prep kit following the manufacturer’s specifications (Illumina Inc., San Diego, CA, USA). Out of the 150 libraries ...
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bioRxiv - Genomics 2021Quote: DNA and RNA extracted from filtered water samples were diluted to a common concentration (1ng/ul) and sent for 16S rRNA amplicon sequencing on an Illumina MiSeq (Illumina, San Diego, CA, USA) according to Earth Microbiome protocols ...
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bioRxiv - Genomics 2021Quote: ... Paired-end sequencing (250 bp) was performed for each library on a MiSeq instrument using 500 cycles v2 chemistry cartridges (Illumina, San Diego, CA, USA).
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bioRxiv - Genomics 2021Quote: ... Paired-end libraries were constructed from the double stranded cDNA by using Illumina DNA Prep with Enrichment kit (Illumina Inc., San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Paired-end sequencing libraries were constructed with an insert size of 500 bp using a TruSeq DNA PCR-Free kit (Illumina, San Diego, CA, USA) according to Illumina library preparation protocols ...
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bioRxiv - Genomics 2020Quote: ... and 8 to 11 kb were generated using the gel selection-based protocol of the Nextera mate pair kit (Illumina, San Diego, CA, USA) and a 0.6% agarose gel in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... We spiked in 1% PhiX control (PhiX Control v3) in a 12 pM library and sequenced on an Illumina MiSeq platform (Illumina, San Diego, CA, USA) using a MiSeq Nano Reagent Kit v2 (500 cycles).
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bioRxiv - Genetics 2020Quote: ... The two resulting PCR products were mixed in equimolar amounts and each sample was barcoded according to the Nextera XT library preparation protocol (Illumina, San Diego, CA, USA). Sequencing was performed on the Illumina MiSeq platform (DNA Core Facility ...
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bioRxiv - Genetics 2020Quote: ... were submitted to GeneSeek (Neogene, Lincoln, NE, USA) for SNP genotyping on the Illumina Infinium Feline 63K iSelect DNA Array (Illumina, San Diego, CA, USA) [21] ...
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bioRxiv - Cancer Biology 2021Quote: ... sequencing libraries using total RNA generated from frozen tissues with a TruSeq mRNA Library Preparation Kit using poly-A-enriched RNA (Illumina, San Diego, CA, USA) and enriched with the human kinome DNA capture baits (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Poly-A selected strand specific library construction and paired-end sequencing (2x 125 bp on a HiSeq 2500 using v4 chemistry; Illumina, San Diego, CA, USA) was performed at the VBCF NGS Unit (Vienna ...
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bioRxiv - Immunology 2022Quote: ... variable regions V3 to V4 of the bacterial 16S ribosomal RNA gene were amplified by limited cycle PCR and subjected to the Illumina 16S metagenomic sequencing library preparation workflow according to the manufacturer’s recommendations (Illumina Inc., San Diego, CA, USA). The obtained DNA libraries were quantified ...
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bioRxiv - Cancer Biology 2022Quote: ... and sequenced on the Illumina NextSeq 2000 sequencer with the P3-200 cycle reagent kit in order to generate approximately 50M million 100-base read pairs per sample (Illumina, Inc., San Diego, CA).
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were pooled and sequenced single-end (1×75) on an Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA) at a sequencing depth of 23-31 million reads ...
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bioRxiv - Cell Biology 2022Quote: ... as described in the section “Quantitative real-time PCR.” The library was prepared using the TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions and sequenced on an Illumina NovaSeq 6000 platform in the 101 bp single-end mode ...
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bioRxiv - Genomics 2022Quote: ... Short-read sequencing data for error correction was generated from the same individual using the TruSeq Nano DNA Low Throughput Library Prep Kit and IDT for Illumina – TruSeq DNA UD Indexes (Illumina, San Diego, CA, USA). Standard protocols were followed to yield a paired library with a 350 bp insert size and 50 bp standard deviation ...
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bioRxiv - Genomics 2022Quote: ... A total of 100 ng of RNA was used as input to the mRNA sequencing library prep kit (TruSeq RNA Access Library Prep Kit by Illumina, San Diego, CA, USA) according to the protocols of the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... The concentration of amplified cDNA was measured with Qubit and 1 ng was used as input for Nextera XT (Illumina, San Diego, CA, USA) library preparation using New P5 SMART PCR oligo and Nextera index oligos ...
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bioRxiv - Genetics 2022Quote: ... were captured by xGen Exome Research Panel v2 (Integrated DNA Technologies, Coralville, Iowa, USA) and sequenced with Novaseq 6000 (Illumina, San Diego, CA, USA) as 150 bp paired-end reads ...
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bioRxiv - Genomics 2022Quote: ... the sequencing was performed with paired-end sequencing of 150nt each end on one lane of Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA) per sample ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was used for cDNA synthesis and purification using a low-throughput protocol with a TruSeq Stranded RNA LT Kit (Illumina, San Diego, CA, USA). A half-scale reaction of the standard protocol was used for library preparation ...
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bioRxiv - Plant Biology 2022Quote: Pair-end library preparation for MiSeq sequencing was conducted using the two-step tailed PCR method described on Illumina (Illumina, San Diego, CA, USA). We used the following primer pairs to amplify partial sequences of the 16S rRNA gene:
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bioRxiv - Microbiology 2022Quote: ... with up to 100 ng DNA input following the manufacturers protocol using internal dual-index 8 bp barcodes with Nextera® adapters (Illumina, San Diego, CA). All libraries were quantified with TapeStation®(Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... A total of 21 isolates were selected (one representative from each cluster) for whole-genome sequencing using the Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA) platform ...
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bioRxiv - Microbiology 2022Quote: ... These programs cover “Tagment Genomic DNA” to “Amplify DNA” in the protocol (Mantis-Nextera DNA Flex library prep protocol, Illumina, San Diego, CA, USA) and “Clean Up Libraries” in the protocol (Epmotion - Library Clean Up protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared from purified RNA (∼1 μg) using Illumina TruSeq standard mRNA library preparation kit as per manufacturer protocol (Illumina Inc., San Diego, CA). Briefly ...
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bioRxiv - Physiology 2022Quote: ... and used to construct stranded RNA-seq libraries from polyA-selected total liver RNA using an Illumina stranded TruSeq mRNA Prep Kit (Illumina, San Diego, CA, USA). Three pooled RNA samples were prepared for each of the four treatment groups ...
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were fragmented and tagged with the indexed adaptors using the Nextera DNA XT Sample Preparation Kit (Illumina, San Diego, CA, USA). The sequence library was quantified by using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... All samples showed a high RNA quality (RNQ>8) and were sequenced using an Illumina® HiSeq 3000 sequencer (Illumina, San Diego, CA, USA). Reads were mapped to the Sscrofa11.1 assembly of the Swine Genome Sequencing Consortium (SGSC ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were pooled and sequenced (single-end 75 bp reads) on Illumina NextSeq 500 using High Output V2 Kit (Illumina, San Diego, CA, USA). Sequencing data were analyzed as described previously (70 ...
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bioRxiv - Genomics 2022Quote: ... 500 ng per sample of isolated DNA was bisulfite converted and hybridized with the Infinium Mouse Methylation BeadChip (Illumina Inc., San Diego, CA, USA), from 24 C57BL/6 mice (all female ...
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bioRxiv - Microbiology 2022Quote: ... Amplicons of the V3-V4 region of the 16S rRNA gene and the and the ITS1 region and library prep were generated by Herculase II Fusion DNA Polymerase Nextera XT Index Kit V2 (Illumina, San Diego, CA, USA). The products of the library prep were validated by an Agilent Tapestation D1000 (Santa Clara ...
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bioRxiv - Microbiology 2022Quote: ... Indexed amplicons were pooled in equimolar amounts and sequenced using Illumina’s v3 600-cycle (paired-end) reagent kit on an Illumina MiSeq benchtop sequencer (Illumina Inc., San Diego, CA, USA) after all DNA extraction blanks and PCR reagent blanks were confirmed for negative amplification.
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bioRxiv - Immunology 2022Quote: ... sequencing was performed by the Biomedical Sequencing Core Facility of the Center for Molecular Medicine (Center for Molecular Medicine, Vienna, Austria) on an Illumina HiSeq 3000/4000 (Illumina, San Diego, CA, USA). For donor 1 ...
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bioRxiv - Microbiology 2022Quote: ... Illumina shotgun libraries were prepared using the Nextera XT DNA Sample Preparation Kit and subsequently sequenced on a MiSeq system with the reagent kit v3 with 600 cycles (Illumina, San Diego, CA, USA) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... A second short-run PCR was performed in order to ligate the Illumina i7 and i5 barcodes (Nextera XT, Illumina, San Diego, CA, USA), and amplicons were purified again as above ...
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bioRxiv - Plant Biology 2022Quote: ... The GBS library was diluted to 3.6 pM and sequenced on one lane (single end, 101 base pair read length) of an Illumina HiSeq 2500 (Illumina Inc, San Diego, CA) at the Genomics Resources Core Facility (Weill Cornell Medicine ...
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bioRxiv - Neuroscience 2022Quote: ... Sequencing libraries were prepared by transposase-assisted tagmentation and enrichment of 3’end of genes using Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA, USA). The libraries were sequenced on the HiSeq2500 for 25 and 75 cycles paired-end reads ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing was performed by the NIDDK Genomics Core facility using a MiSeq system with the single-end 50 bp Sequencing Kit (Illumina, San Diego, CA, USA). Sequence reads were mapped to the reference genome (NC_000913.3 ...
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bioRxiv - Plant Biology 2022Quote: ... Illumina sequencing pair-end libraries with an insert size of 300 bp were prepared using the Nextera DNA Flex Library Prep Kit (Illumina, San Diego, CA, USA). After the libraries were constructed ...
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bioRxiv - Microbiology 2023Quote: ... The final pooled library was submitted for high throughput sequencing on the Illumina MiSeq platform using the PE250 V2 chemistry method (Illumina, San Diego, CA, USA). For this work ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg total RNA was used for preparation of cDNA libraries with the TruSeq Small RNA Library Preparation Kit (Illumina, San Diego, CA, USA). Libraries were sequenced as 50 bp paired-end reads on an Illumina Genome Analyzer ...
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bioRxiv - Plant Biology 2022Quote: ... Both ends of the cDNA libraries were sequenced for 60 cycles using a paired-end module with an Illumina NextSeq 500 platform (Illumina, San Diego, CA, USA). Two biological replicates were conducted for each experiment.
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bioRxiv - Microbiology 2022Quote: ... Whole genome metagenomic libraries were prepared at LLNL using the Illumina DNA Flex library kit (now called Illumina DNA Prep, Illumina Inc., Santa Clara, CA) using 1 ng of sample DNA and 12 cycles of amplification ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 1 ug of total RNA was then used to prepare small RNA library according to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: RNAseq was performed as single-end sequencing for a read length of 75 bp with an Illumina NextSeq 500 sequencer (Illumina, San Diego, CA, USA) in high output runs using a NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs ...