Labshake search
Citations for Bioline :
401 - 420 of 420 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... Converted DNA was subjected to 12 cycles of PCR amplification to enrich for adapter-ligated fragments with MyTaq Mix (Bioline Inc.), during which each sample was also barcoded with unique sequence tags ...
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bioRxiv - Cell Biology 2023Quote: ... the embryos were individually scraped off the microscope slide using drawn-out glass pipettes (new pipette for each embryo) filled with 1X MyTaq Red mix (Bioline, #25044), aspirated and transferred into 10 µL of DNA lysis buffer and processed for genotyping as described above.
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bioRxiv - Genomics 2020Quote: ... DNA was extracted using the NucliSENS® EasyMag® (BioMérieux, Marcy l’Etoile, France) or the Isolate II Genomic DNA kit (Bioline, Tennessee, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... SK-MEL-147 and SK-MEL-23 melanoma cells expressing either sgCtrl or sgSTUB1 was isolated using the Isolate II RNA Mini Kit (Bioline, BIO-52072) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: DNA was extracted from whole female BF post adult and pupal injection using an Isolate II Genomic DNA extraction kit (Bioline, NSW, Australia). Six flies were assayed at each point of time for determination of the relative Wolbachia density ...
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bioRxiv - Synthetic Biology 2022Quote: ... Amplicons of the appropriate size range for the transgene of interest were then gel extracted for nanopore sequencing using the ISOLATE II PCR and Gel Kit (Bioline, #BIO-52060) (Figure S5).
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bioRxiv - Immunology 2023Quote: ... the aqueous phase was mixed 1:1 v/v with 70% ethanol and loaded into a column of the Isolate II RNA Mini kit (Bioline, Cincinnati, OH). Total RNA was purified following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2023Quote: ... The samples were collected 72 hours after the addition of the drugs and genomic DNA (gDNA) was extracted using the ISOLATE II genomic DNA kit (Bioline, BIO-52067). The samples were then quantified using Nanodrop and 200 ng of gDNA was used in the library PCRs that were performed as for the screening above ...
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bioRxiv - Cancer Biology 2023Quote: The isolation of total RNA and its conversion to cDNA were performed using the ISOLATE II RNA Mini Kit (Bioline, BIO-52073) and the qScript cDNA synthesis kit (Quanta Bioscience ...
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bioRxiv - Immunology 2024Quote: Parasite genomic DNA was isolated from mouse peritoneal exudate cells and whole brain using the Isolate II Genomic DNA Kit (Bioline, BIO-52067). Prior to isolation ...
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bioRxiv - Cell Biology 2024Quote: RNA from cell pellets treated with dTAG-13 for four hours was isolated using the Isolate II RNA Mini Kit (Bioline BIO-52072), and 1 μg was converted to cDNA using the qScript cDNA Synthesis Kit (VWR 101414) ...
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bioRxiv - Microbiology 2019Quote: ... A second round of amplification was performed by transferring 5μl of 1:100 diluted Round 1 product into 15μl Bioline MyFi™ Mix (BIOLINE GmbH, Luckenwalde, Germany) reactions.
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bioRxiv - Cell Biology 2020Quote: ... 25 ng cDNA were used in a total reaction mix of 20 μl containing 10 ul Sensi Fast SYBR Green Hi-Rox (Bioline, London, UK) as well as 400 nM forward and reverse primer (Supplementary Material and Methods ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR amplicon band was cut from the gel and isolated by PCR Isolate II PCR and Gel Kit (Bioline, cat. no. BIO-52060) and lastly bead-purified ...
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bioRxiv - Genomics 2023Quote: ... The PCR product was cut from gel to remove any other undesired products and cleaned with the PCR Isolate II PCR and Gel Kit (Bioline, cat. no. BIO-52060). The isolated samples were sequenced by Illumina MiSeq for screen 1 (14.3 million reads ...
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bioRxiv - Physiology 2021Quote: ... cDNA products were used as templates for qRT-PCR assessment of gene expression using the SYBR green system (SensiFast™ Sybr No-Rox Mix; Bioline, London, UK) on a Bio-Rad CFX384 Real-Time system instrument (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The final product was diluted to 1:20 and 2 µL of the dilution were used in each 10-µl RT-qPCR reaction along with 400 nM of primers gene- or isoform-specific primers (Table S4B) and 1X SensiFAST SYBR No-ROX Mix (Bioline, cat#BIO-98005). Quantitative PCR was performed in CFX384 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... Single organoids were hand-picked with capillary pipet tips and examined for locus deletion by PCR using MyTaq™ Red Mix (Bioline, #BIO-25043) and the following cycling parameters ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9 target sites within the AAVS1 locus were PCR amplified in a 25 or 50 μL reaction volume per sample using high-performance RANGER PCR Mix (Bioline USA, Inc., Memphis, TN, USA) or PlatinumTM SuperFiTM PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: The full exon 3 of PRNP gene (771 bp) of all animals was amplified by PCR using MyTaq™ Red Mix (Bioline Meridian Life Sciences-BIO-25043). Briefly ...