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Citations for Bioline :
301 - 350 of 420 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: RNA was isolated from cells on with the Isolate II RNA Mini Kit (Bioline, BIO-52702). 1 μg was converted to cDNA with qScript (Quanta ...
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bioRxiv - Genomics 2022Quote: ... frozen samples were harvested and extracted using the ISOLATE II Plant miRNA kit (Bioline, Sydney, Australia) as per the manufacturer’s instructions ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... RNA was extracted using ISOLATE II RNA Mini kit as per manufacturer instructions (Bioline BIO-52072) and RNA concentration and quality determined by Nanodrop (Thermo Fisher) ...
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bioRxiv - Genomics 2023Quote: Genomic DNA was extracted using either the Bioline Isolate II Genomic DNA Kit (Bioline, Eveleigh, Australia) following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR was performed using C1000 Touch Thermal Cycler (BioRed) and SYBR mix (Bioline, GmbH, Germany), using validated primer sets (Herber et al ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was carried out using 5x PyroTaq qPCR mix Plus EvaGreen (CMB Cultek Molecular Bioline) in a QuantStudio5 machine (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... the 15 μl PCR reaction mixture contained 1x MyTaq™ Red PCR Mix (Bioline, South Africa), 0.2 μM primers ...
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bioRxiv - Plant Biology 2021Quote: ... Each 20 μL reaction consisted of 10 μL of SensiFAST Probe No-ROX Mix (2×) (Bioline), 0.25 μM each of RamF6 and RamR6 primers (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Four oligonucleotides were designed [40] for PCR using MyTaq Red Mix 1X (Bioline, Cat#BIO-25043), according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... For qPCR reaction 1 μg of cDNA was used with SensiFast Sybr Lo-Rox mix (Bioline) and respective primer pair ...
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bioRxiv - Immunology 2022Quote: ... 3 µL of 5x PyroTaq EvaGreen qPCR Mix Plus with ROX (Cultek Molecular Bioline, Madrid, Spain), and transcript-specific forward and reverse primers at a 10 μM final concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... target regions were amplified by PCR using 50 ng genomic DNA in MyTaq Red mix (Bioline) according to manufacture instructions (primers are shown in Table S3) ...
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bioRxiv - Plant Biology 2022Quote: ... qPCR reactions were carried out using PyroTaq EvaGreen mix Plus (ROX; CulteK Molecular Bioline, Madrid, Spain) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and qPCR was performed as described (18) using SensiFast Sybr Lo-Rox Mix (BIO-94020, Bioline). The run was performed by using the Applied Biosystems (Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... A typical qPCR reaction contained 5 μl 2×SensiFAST SYBR No-Rox mix (Bioline #BIO-98080), 2∼10 ng DNA template ...
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bioRxiv - Genomics 2023Quote: ... PCR2 was performed using 15 μL 2 x MyTaq Red Mix (Bioline, cat. no. BIO-25044) and 5 μL of each PCR1 product with well-specific Illumina PCR indexed primers (TAC0159 & TAC0009 ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was carried out using 5x PyroTaq qPCR mix Plus EvaGreen (CMB Cultek Molecular Bioline) in a QuantStudio5 machine (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: PCR products were purified using a commercial kit (Isolate II PCR and Gel Kit, Bioline, London, UK) and sent for sequencing (Macrogen Europe ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was isolated from at least 10 midguts using the Isolate II RNA Mini kit (Bioline, UK). The extracted RNA was quantified using Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was isolated from sorted cells using the Isolate II RNA mini kit (Bioline, London, UK) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted from MSCs and OA-HSFs using the Isolate II RNA Micro Kit (Bioline, Canada) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... meristems were snap-frozen and total RNA was extracted using Isolate II RNA plant kit (Bioline, UK). RNA-sequencing libraries were synthesised for each sample using NEBNext Ultra II stranded RNA library synthesis kits ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from PCa cells using the Isolate II RNA Mini Kit (Bioline, London, UK) and was reverse transcribed to cDNA using the SensiFastTM cDNA synthesis kit (Bioline ...
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bioRxiv - Immunology 2023Quote: ... and on-column DNase treatment and purification were performed using the ISOLATE II RNA mini kit (Bioline) or RNeasy Mini kit (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: ... The 15 μl PCR cocktail consisted of 7.5 μl of PCR MyTaq Red Mix (Bioline, GmbH, Germany), 1.0 μl of genomic DNA template (70 ng/μl) ...
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bioRxiv - Genomics 2019Quote: ... 8 µl of DpnII-digested products was amplified by PCR with MyTaq Red Mix (Bioline #BIO-25044) and 1.25 µM primers Adr-PCR-Rand1 in a total volume of 40 µl ...
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bioRxiv - Microbiology 2021Quote: ... The 15 µl PCR reaction mixture contained 1x of MyTaq ™ Red PCR Mix (Bioline, South Africa), primers at 0.2 µM ...
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bioRxiv - Cancer Biology 2022Quote: PCR reactions were performed in 25 μl reactions using the MyTaq Red Mix (Bioline, Meridian Life Science). The RT-PCR reaction conditions were optimised for each primer pair and are designated as Condition A to D ...
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bioRxiv - Plant Biology 2021Quote: ... Each 20 μL reaction consisted of 10 μL of SensiFAST Probe No-ROX Mix (2×) (Bioline, Australia), 0.25 μM of each forward and reverse primer (Integrated DNA Technologies ...
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bioRxiv - Genetics 2022Quote: Genotyping was performed using standard PCR protocols for MyTaq HotStart Red Mix (Bioline BIO- 25048, Memphis, TN) with primers listed in Supplementary Table 7.
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bioRxiv - Molecular Biology 2020Quote: ... Both PCR reactions were carried out with 25 µl MyTaq Red mix (Bioline cat. no. BIO-25044), 0.5 µM of each primer and 50 µl final volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 8 μl of DpnII-digested products was amplified by PCR with MyTaq Red Mix (Bioline #BIO-25044) and 1.25 μM primers Adr-PCR-Rand1 in a total volume of 40 μl ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplex PCR amplification was performed in a 10 μL reaction containing 1X MyTaq HS Red Mix (Bioline), 10 ng DNA template ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from treated cells via an ISOLATE II RNA mini kit (Bioline, Cat: BIO-52073) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Bioline Isolate II PCR and Gel kit (Bioline, Cat. No. BIO-52060). Samples were sequenced using the Illumina Miseq platform ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesised using SuperScript III reverse transcriptase from RNA extracted using ISOLATE II RNA Mini Kit (Bioline) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Bands of correct size were cut out and purified using the Isolate II PCR and gel kit (Bioline). 50 ng of vector and 150 ng of each fragment were mixed with 3 μl of HiFi DNA Master Mix (NEB ...
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bioRxiv - Molecular Biology 2019Quote: DNA was extracted from surface-sterilized whole insects using the ISOLATE II Genomic DNA Kit (Bioline, London, UK) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... PCR amplicons of each sample were pulled together and purified using Isolate II PCR and gel Kit (BIOLINE) and about 100 ng of DNA was used in library preparation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA from historical museum toepad samples was extracted using the Bioline Isolate II Genomic DNA extraction kit (Bioline), but suspending tissues in 400 μL lysis buffer with 25 μL proteinase k and incubated overnight at 55 °C ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons of appropriate size were excised and purified using an Isolate II PCR and Gel kit (Bioline, UK). The purified amplicons were included in cycle sequence reactions ...
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bioRxiv - Microbiology 2022Quote: Faecal DNA was extracted from FecalSwabTM media (200µL) using the ISOLATE II Fecal DNA Kit (Bioline, Sydney, Australia) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Isolation of total RNA and cDNA synthesis was performed by using the Isolate II RNA Mini Kit (Bioline) and SensiFAST cDNA Synthesis Kit (Bioline) ...
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bioRxiv - Plant Biology 2023Quote: ... The total RNA was extracted from ground root samples using an ISOLATE II Plant Mini Kit (Bioline, Australia), cDNA was synthesised from 1ug of RNA using a SensiFAST cDNA Synthesis Kit (Bioline ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted from parasite lines using an Isolate II Genomic DNA Kit (Bioline, Meridian Bioscience Australia), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Correct sized amplicons were then purified using an Isolate II PCR and Gel Kit (Bioline, Meridian Biosciences, Australia) following the manufacturer’s instructions for PCR clean-up and sent for Sanger sequencing at the Victorian Clinical Genetics Services (VCGS ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed with 50 ng of cDNA in each well and SensiFAST probe no-ROX mix (Bioline) using a CFX Connect system (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... using 3 ng of cDNA per reaction and Sensifast SYBR No-ROX mix (Bioline Corporation, Alvinston, ON, Canada). Complementary DNA of mRNA was amplified using a pair of specific forward and reverse primers (Supplementary Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 μl of 86.6% (5:1) diluted MyTaq HotStart Red Mix in water (Bioline, cat. no. BIO-25048) and 10 μl of 1 μM of each primer (TAC0007 and TAC0012 final concentration of 200 nM ...
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bioRxiv - Microbiology 2021Quote: ... After PCR verification in agarose gel and PCR clean-up using the ISOLATE II PCR and Gel Kit (Bioline), the remaining plasmid template was digested with DpnI at 37°C for 3h ...