Labshake search
Citations for GE Life Sciences :
351 - 400 of 1108 citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and applied onto GSTrap FF 5 mL (GE Healthcare, USA) using a buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Plant Biology 2022Quote: ... The clarified cell lysate was first injected onto a 5 ml Ni2+-NTA column (GE Healthcare). The complexes were step-eluted with elution buffer (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell lysates prepared by sonication and centrifugation were purified on a HisTrap HP column (GE Healthcare). The N-terminal polyhistidine-GST tag was cleaved by incubation with TEV protease at 4 °C overnight ...
-
bioRxiv - Molecular Biology 2023Quote: Lysates were purified using a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) connected to an ÄKTA pure protein purification system (Cytiva) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare) equilibrated in 200 mM potassium phosphate ...
-
bioRxiv - Biophysics 2023Quote: ... clarified lysate obtained from the cells was bound to a HiTrap Chelating HP column (GE Healthcare) in low imidazole buffer (50 mM PIPES ...
-
bioRxiv - Microbiology 2023Quote: ... lysates were incubated 30 min with chelating Sepharose Fast Flow beads charged with Ni2+ (GE Healthcare). Beads were then successively washed in the washing buffer (20 mM Tris-HCl ...
-
bioRxiv - Genomics 2023Quote: ... Lysates were pre-cleared with 30µl of either protein-A-sepharose (GE Healthcare, 17-5280-01) or protein-G-sepharose beads (GE Healthcare ...
-
bioRxiv - Molecular Biology 2023Quote: ... the clarified lysate was added to a 5 ml HisTrap High Performance column (GE Healthcare/Cytiva), washed with 5 column volumes of lysis buffer supplanted with 6 M urea ...
-
bioRxiv - Cell Biology 2023Quote: ... Dynein complexes were captured from Sf9 cell lysates using IgG Sepharose 6 FastFlow beads (GE Healthcare), labeled with SNAP-Cell-TMR-Star ...
-
bioRxiv - Biochemistry 2023Quote: ... the cleared lysate was incubated with 500 µL glutathione agarose slurry (GE Healthcare, #17-0756-01) (resuspended 1:1 in lysis buffer ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1000 μg of cell lysates were incubated overnight with Glutathione Sepharose™ beads (GE Healthcare, UK) at 4 °C on a rotator ...
-
bioRxiv - Biophysics 2024Quote: ... The supernatant of the cell lysate was loaded onto a HisTrap column (5 mL-GE Healthcare) and the bound protein was eluted with an imidazole gradient (0-1 M) ...
-
bioRxiv - Biochemistry 2023Quote: ... The soluble cell lysates were applied on a Ni-sepharose excel (GE Healthcare, #17-3712-01), packed in a column of 4 ml bed volume with Sl1 buffer buffer (20mM Tris ...
-
bioRxiv - Plant Biology 2022Quote: ... diluted 1:10,000 in 2% TBST with 2% Blocking (GE Healthcare), was applied for 1 h.
-
bioRxiv - Microbiology 2024Quote: ... 2 µMnt ssDNA (Ovio 45) and 2 mM ATP (GE Healthcare) in a 40 µL reaction solution containing 10 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lysates 30-50 ug were run on SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare). Western blots were conducted using anti-CTLA-4/CD152 (LS C193047 ...
-
bioRxiv - Microbiology 2019Quote: ... Clarified lysate was loaded onto a 5 mL His-Trap Ni-NTA column (GE Healthcare Life Sciences) using an Akta Pure (GE Healthcare Life Sciences) ...
-
bioRxiv - Cell Biology 2019Quote: ... The lysate was cleared by centrifugation and applied to a 5ml GSTrap FF column (GE Healthcare, 17513101) to initially purify GST-proteins ...
-
bioRxiv - Biochemistry 2021Quote: ... The target was captured from the cleared lysate on a 5 ml HisTrap FF column (GE Healthcare) and eluted with a linear gradient from 10 to 250 mM imidazole ...
-
bioRxiv - Biochemistry 2020Quote: ... Cell lysates were supplemented with 10 mM imidazole and incubated with Ni-Sepharose excel resin (GE Healthcare) for 2 that 4 °C ...
-
bioRxiv - Biochemistry 2020Quote: ... The cell lysate was centrifuged and the supernatant was incubated with Ni-NTA affinity resin (GE Healthcare). The target protein was eluted with lysis buffer supplemented with 300 mM imidazole ...
-
bioRxiv - Biophysics 2021Quote: Purified protein or cell lysates were injected onto a Superose 6 increase 5/150 column (GE Healthcare), running in a buffer containing 20 mM Tris (pH 7.5) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cell-free lysate was applied into a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated in start buffer ...
-
bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD was purified from the clarified lysate at 4°C using an ÄKTA Pure (GE Healthcare) in one day ...
-
bioRxiv - Biochemistry 2020Quote: ... After cell disruption the cleared lysate was loaded on a 5 ml HisTrap FF column (GE Healthcare) equilibrated in lysis buffer A and the protein was eluted with a gradient of 25 – 250 mM imidazole ...
-
bioRxiv - Microbiology 2021Quote: ... The lysate was filtered (1.2 μm pore size, GF/C, Whatman, GE Healthcare, Little Chalfont, United Kingdom), acidified to pH ≤ 1 for sterilization using 30% hydrochloric acid ...
-
bioRxiv - Molecular Biology 2021Quote: ... Resulting lysate was clarified by centrifugation and loaded onto HisTrap crude column charged with NiSO4 (GE Healthcare). Unbound proteins were washed out with buffer A supplemented with 10 mM imidazole ...
-
bioRxiv - Cell Biology 2022Quote: ... The clarified lysates were incubated with glutathione sepharose (1 ml bed volume; GE Healthcare #17-0756-01), washed and eluted by the addition of 2 x 1.5 ml of 10 mM reduced L-glutathione (Sigma #T6066 ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant of cell lysate was purified over Glutathione-Sepharose 4B (GS4B) (GE Healthcare, Boston, MA, USA) or Ni-NTA resins (Qiagen) ...
-
bioRxiv - Biochemistry 2019Quote: ... Cleared lysates were supplemented with 1 mM DTT and incubated with GSH-Sepharose 4B matrix (GE Healthcare) for 1 h at 4 °C ...
-
bioRxiv - Molecular Biology 2019Quote: ... The lysate was then subjected to first round of affinity purification with IgG-SepharoseTM (GE healthcare, Germany) for 1 hour at 4°C on the rotating wheel ...
-
bioRxiv - Microbiology 2019Quote: ... The cleared lysate was loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (50 mM Hepes pH8 ...
-
bioRxiv - Biochemistry 2019Quote: ... Clarified lysate was filtered through three sets of filters with decreasing pore size: 1.2 µm (GE Healthcare), 0.8 µm (GE-Healthcare ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The lysate was loaded on a Ni affinity step on HisTrap FF crude column (GE Healthcare, Inc.) and the protein was eluted at a concentration of 150 mM imidazole ...
-
bioRxiv - Microbiology 2021Quote: Cell lysates were separated using 8-12% polyacrylamide gels and transferred to Amersham Nitrocellulose Membranes (GE healthcare) via Trans-blot Turbo Transfer system (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The lysates were cleared by centrifugation and loaded onto a 5 ml HisTrap HP column (GE Healthcare). After washing with buffer A (50 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2021Quote: ... The cleared lysate was subjected to a histidine affinity chromatography in a HisTrap HP column (GE Healthcare) equilibrated in Buffer A ...
-
bioRxiv - Biophysics 2021Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system as follows (GE Healthcare). First ...
-
bioRxiv - Microbiology 2020Quote: ... The PhuS or PhuS H212R mutant lysate was applied to a Sepharose-G column (GE Life Sciences) equilibrated with 20 mM Tris-HCl (pH 8.0 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was filtered (0.8 µM) and applied to a 10 ml HisTrap FF (GE Healthcare) equilibrated in washing buffer (lysis buffer without protease inhibitors) ...
-
bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was filtered (0.8 µM) and applied to a 5 ml GSH column (GE Healthcare) equilibrated in washing buffer (lysis buffer without DNAse and PMSF) ...
-
bioRxiv - Cell Biology 2019Quote: ... 250 μL of cleared lysate was fractionated on Superose6 Increase 10/30 gel filtration column (GE Healthcare) equilibrated in running buffer (25 mM Tris pH 8.0 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The lysate was passed over a self-packed Ni2+-charged IMAC Sepharose 6 Fast Flow (GE Healthcare) column ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lysates were separated by 4-20% SDS-PAGE and transferred to PVDF membranes (GE Healthcare, Piscataway, NJ). Membranes were blocked with 5% BSA in 1x TBS with 0.1% Tween 20 for 1 hour at room temperature with shaking ...
-
bioRxiv - Biophysics 2020Quote: ... the cleared lysate was loaded at 4 °C on an HisTrap HP Ni2+–Sepharose column (GE Healthcare) following the manufacturer’s instructions and further purified by size exclusion chromatography (SEC ...
-
bioRxiv - Immunology 2020Quote: ... The soluble fraction was purified from the cell lysate using Glutathione Sepharose 4B beads (GS4B, GE Healthcare). The proteins were then digested with PreScission protease (GE Healthcare ...
-
bioRxiv - Cell Biology 2021Quote: ... The protein was purified from cleared cell lysate using Ni-sepharose 6 Fast flow resin (GE Healthcare) in a poly-prep column (Bio-Rad) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell lysates were injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences). To slow Fe2+-oxidation and precipitation in equilibration buffer ...
-
bioRxiv - Microbiology 2022Quote: ... The cleared lysate was loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare) equilibrated in Buffer A (50 mM Hepes pH8 ...