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Citations for GE Life Sciences :
301 - 350 of 1108 citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Cell lysates were mixed with 400 µl IgG SepharoseTM 6 Fast Flow slurry (GE Healthcare) pre-equilibrated with TNM100 buffer (50 mM Tris-HCl pH 7.8 ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... Clarified lysates were subjected to affinity chromatography using a GSTrap HP affinity column (GE Healthcare). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was loaded onto a HisTrap HP 5 ml (GE Healthcare Life Sciences, USA) column equilibrated with buffer A and washed with the same buffer ...
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bioRxiv - Microbiology 2020Quote: ... cells were treated with trypsin to remove any virus that had not entered the cell and then cushioned onto Ficoll-Pacque (GE Healthcare Life Sciences) to remove residual virus and debris ...
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bioRxiv - Biochemistry 2021Quote: ... and the released virus particles were purified by 55% cesium chloride and desalted through a PD10 column (GE Healthcare Life Sciences, Cat#17085101). Adenovirus particles were tail vein injected at doses of 0.1 OD for the lean ...
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bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...
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bioRxiv - Molecular Biology 2023Quote: ... was processed for amplification of complete genome of the virus by rolling circle amplification (RCA) method using TempliPhi illustra amplification kit (GE Healthcare, Piscataway, NJ). The resulting RCA product (2 μL ...
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bioRxiv - Neuroscience 2024Quote: ... The pellet was added to lysed HEK cells prior to virus purification through heparin affinity columns (HiTrap Heparin HP, GE Healthcare, Uppsala, Sweden) and concentrated using Amicon Ultra-4 centrifugal filters (Millipore ...
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bioRxiv - Biophysics 2021Quote: ... The protein was purified from the lysate using Ni2+-NTA column of [KTA Pure (GE healthcare). For in vitro biotinylation of the polyprotein ...
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bioRxiv - Microbiology 2021Quote: ... lysates were incubated 30 min with chelating Sepharose Fast Flow beads charged with Ni2+ (GE Healthcare). Beads were then successively washed in the washing buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The cell lysate was incubated with 10 ml of Glutathione Sepharose 4B resin from GE Healthcare, equilibrated with the same buffer for 2 hours ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Bacterial cell lysates were then loaded onto a HiTrap Q HP anion exchange column (GE Healthcare) and were then equilibrated with 20 mM Tris/0.5 mM EDTA (pH 8.3 ...
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bioRxiv - Molecular Biology 2022Quote: ... H-NS containing cell lysate was directly applied to a HiTrap Heparin HP column (GE Healthcare) equilibrated with Binding Buffer with 130 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was then passed through a 1ml HisTrap column (GE healthcare, Little Chalfont, UK). For every 2 ml of cell lysate passed though the column ...
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bioRxiv - Biochemistry 2019Quote: ... The resultant lysate was loaded onto a 7.5 mL nickel SepharoseTM fast flow column (GE Healthcare), pre-equilibrated with lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were separated on a Superose 6 10/300 column (Ge Healthcare, Waukesha, WI, USA) pre-equilibrated with 20mM Tris–HCl pH7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysate was applied to a 5 ml HiTrap HP Q column (GE Healthcare Biosciences). The flow-through ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cleared by centrifugation and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–250 mM in 30 column volumes ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were incubated with 100 μl of Streptavidin-Sepharose beads (GE Healthcare; 17-5113-01) for 3 h at 40°C ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was cleared by ultracentrifugation and supernatant was incubated with GST-4B beads (GE Life Sciences) for 1H ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 1 mL IgG Sepharose beads suspension (6 Fast Flow, GE Healthcare) pre-rinsed with buffer for 20 ml lysate on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... The clarified cell lysate was first injected onto a 5 ml Ni2+-NTA column (GE Healthcare). The MBP-OsHIPP19-HMA/6xHis-SUMO-AVR-PikF complex was step-eluted with elution buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates and samples were separated by SDS–PAGE and transferred to PVDF membrane (GE Healthcare) using wet transfer and Towbin blotting buffer (25 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... The lysate soluble fraction was loaded onto a 5 mL HiTrap chelating HP column (GE Healthcare) immobilized with 100 mM cobalt chloride and equilibrated with the lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared via centrifugation and loaded to His Spin Trap spin column (GE Healthcare). The column was sequentially washed with PBC containing 20mM ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were electrophoresed in a 10% Acrylamide gel and transferred onto a nitrocellulose membrane (GE Healthcare) using a semi-dry blotter (BioRad) ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysates was subjected to Nickel gravity column using 5 ml His-Trap columns (GE Healthcare), and protein was eluted with 300 mM imidazole in column volumes ...
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bioRxiv - Biophysics 2021Quote: ... The cleared cell lysate was applied to a HisTrap FF Ni-NTA affinity column (GE healthcare) equilibrated with HisA buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysates were loaded onto Ni-Sepharose beads and nutated for ∼1 h (GE Healthcare). Beads were washed twice with denaturing buffer and hexa-histidine-tagged proteins were eluted with the same buffer supplemented with 500 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... The soluble fraction of the lysate was loaded on a nickel column (HisTrap Hp, GE healthcare). The column was extensively washed with wash buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg of cell lysate was incubated with 40 μl of glutathione-Sepharose beads (GE Healthcare) at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... The soluble fraction of the lysate was loaded on a nickel column (HisTrap Hp, GE healthcare). The column was extensively washed with wash buffer containing 20mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the cleared lysate was loaded onto a 5 ml HisTrap FF column (GE Healthcare, Chicago, U.S.A.) connected to an Äkta Purifier FPLC system (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... The lysate soluble fraction was loaded onto a 5 ml HiTrap chelating HP column (GE Healthcare) immobilized with 100 mM cobalt chloride and equilibrated with the lysis buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Lysate was clarified by centrifugation and loaded onto HisTrap™ High-Performance 5 mL (GE Healthcare) columns using a peristaltic pump ...
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bioRxiv - Molecular Biology 2019Quote: ... The soluble fraction of the lysate was loaded on a nickel column (HisTrap Hp, GE healthcare). The column was extensively washed with wash buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... IP is carried out with clarified cell lysate pre-cleaned by protein G sepharose (GE Healthcare), then the cell lysate was incubated with anti-LRRC33 or control mouse IgG (2μg/ml ...
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bioRxiv - Genetics 2021Quote: ... Protein lysates were resolved by 12% SDS-PAGE and transferred to PVDF membrane (GE Healthcare AmershamTM) using a Trans-Blot® SD Semi-Dry Transfer Cell (BioRad) ...
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bioRxiv - Bioengineering 2020Quote: ... 4 °C) and the lysate was loaded onto a StrepTrap HP 5 mL column (GE Healthcare) and purified with the ÄKTA™purifier system (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... lysed and the cell lysate was loaded onto a 1 ml GSTrap HP column (GE Healthcare) before elution using 50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... The cleared lysate was loaded into a pre-equilibrated HisTrap column (GE Healthcare 1mL HisTrap column) using an ÄKTA Pure Protein Purification System (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... The cleared cell lysates were incubated overnight with glutathione-Sepharose 4B beads (GE Healthcare Life Sciences) in the presence of 5 mM dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Affinity purification was carried out by passing the clarified lysate over a GSH column (GE Healthcare) using the ÄKTA Prime Plus system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The filtered cell lysate was loaded onto a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... The proteins were purified from the cell lysate using 300 μl GST resin (GS4B, GE Healthcare), and incubated with excess purified wild-type or mutant rDPP9 proteins on ice for 60 min ...
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bioRxiv - Microbiology 2022Quote: ... Filtered lysates with strep-tagged proteins were applied onto StrepTrap HP 1 ml columns (GE Healthcare). Subsequent protein separation was conducted using the ÄKTA start chromatography system (GE ...
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bioRxiv - Plant Biology 2022Quote: ... the cell lysate was incubated with Ni-NTA beads (Ni Sepharose 6 Fast flow, GE Healthcare) for 45 mins ...
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bioRxiv - Biochemistry 2022Quote: ... lysate was passed through an immobilized metal affinity chromatography (IMAC) column (GE Healthcare HisTrap™ HP) equilibrated with 5 column volumes (CVs ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting clarified lysate was batch-bound to ~1 mL of settled IgG sepharose (GE Healthcare) equilibrated in lysis buffer and allowed to incubate on a roller for 2 hours at 4°C ...