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Citations for GE Life Sciences :
551 - 600 of 1108 citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... After cell lysis by sonication cell debris was removed by centrifugation and the lysate was passed through a His-Trap column (GE Healthcare). Proteins were eluted with a gradient of buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysates were quantified and 50 μg were resolved in 4-20% SDS-PAGE and transferred onto nitrocellulose membranes (GE healthcare). The membranes were then incubated with the primary antibodies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Reduced proteins were now purified from the lysate samples using SP3 magnetic beads (1:1 mixture of SpeedBead Magnetic Carboxylate Modified Particles 65152105050250 and 45152105050250, GE Healthcare). To enable protein binding to the beads ...
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bioRxiv - Neuroscience 2020Quote: ... Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences, Marlborough, MA). After blocking in 5% non-fat dried milk powder (Biorad ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared via ultracentrifugation and his-tagged protein purified on the AKTÄ Pure on the HisTrap HP column (GE Healthcare). Fractions of interest were second round purified via size exclusion chromatography on the Superdex 200 column (GE Healthcare) ...
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bioRxiv - Plant Biology 2021Quote: ... His- or GST-tagged proteins were captured from the lysate by the addition of 100 μL Ni Sepharose High Performance beads (GE Healthcare) or Glutathione Sepharose High Performance beads (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... The bacteria lysate containing GST-Avr1d was incubated with 10 μl glutathione sepharose 4B beads (Cat: 45-000-139, GE Healthcare) for 3 hours and purified by washing with 1×PBS for 5 times ...
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bioRxiv - Systems Biology 2020Quote: ... the target-specific antibody was added to the cellular lysates together with 25 μl of Protein A or G sepharose (GE Healthcare) depending on the species of target antibody and the mixture was incubated overnight rotating at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Fusion proteins were recovered from the cell lysates by conventional affinity chromatography on His-trapTMHP columns (GE Healthcare, 17-5247-01) using an Akta Explorer system (Amersham Pharm BiotechGE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 10 µg of mESC lysate were separated on 4–12% or 10% SDS-PAGE gels (pre-cast NuPage® GE healthcare or Mini-Protean TGX Stain free precast gels ...
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bioRxiv - Cancer Biology 2022Quote: ... cell supernatants/lysates were precleared using 30 μl of a 50% slurry of Protein G Sepharose (GE Healthcare, #17-0618-01), while for IP of pyo-KSR1 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants of the cell lysates were run on 12% resolving Tris-glycine gels and then transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). To detect PstP-strep-tag II ...
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bioRxiv - Biophysics 2022Quote: ... Clarified lysate was passed through a 10 ml Ni2+-Sepharose 6 Fast flow resin (GE Healthcare Catalogue number 17-5318-02), washed with 50 ml of binding buffer (50mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... the cleared lysate was applied to a 10 ml Strep-Tactin column (Iba) and a gel filtration column (Superdex200 16 600, GE Healthcare). The purified protein was stored in PBS containing 1 mM TCEP at -80°C ...
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bioRxiv - Microbiology 2022Quote: ... An equal protein concentration or equal volume of cell and EV lysate was run in an SDS 10% polyacrylamide gel and subsequently transferred onto a nitrocellulose membrane (GE Healthcare). Total protein was measured by Ponceau S stain ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants of cell lysate were incubated with either pre-equilibrated GFP nanobody-conjugated NHS-Activated Sepharose 4 Fast Flow Agarose (GE Healthcare) or Pierce Protein A Agarose (Invitrogen/Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... The remainder of the lysates were incubated with polyclonal rabbit anti-GFP (EU2; Eusera) for 1 hour followed by Protein A-Sepharose beads (GE Healthcare) for another hour at 4° C ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysate was centrifuged at 35,000 g for 60 minutes to remove insoluble cell debris and the soluble lysate was loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
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bioRxiv - Neuroscience 2019Quote: ... After another centrifugation and filtration step the lysate was then loaded onto a combination of a HiTrap Q and a HiTrap SP column (GE Healthcare) pre-equilibrated with running buffer ...
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bioRxiv - Immunology 2020Quote: ... Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare). After purification ...
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bioRxiv - Pathology 2020Quote: ... the vector was purified from the cell lysate using a iodixanol density gradient followed by anion exchange chromatography (HiTrap Q-FF column, GE Healthcare). The scAAV9-CB-SMN vector was finally resuspended and concentrated in DPBS on a centrifugal filter unit (Amicon® Ultra-15 ...
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bioRxiv - Synthetic Biology 2019Quote: ... cells were lysed by three passes through an emulsiflex C5 (Avestin, Mannheim, Germany) and cleared cell lysate was loaded onto a HisTrap FF column (GE Healthcare). After washing with 10 column volumes of buffer A (30 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates with equal total protein amount were incubated with 50 μl pre-washed Glutathione Sepharose 4B (GE Healthcare Life Sciences) in 100 μl GST pull-down buffer (20 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... Cell debris was pelleted at 15000 × g for 10 min and 10 μg of the cleared cell lysates were resolved on a 10% SDS-PAGE and transferred to 0.45 μm nitrocellulose membrane (GE life Sciences). Membranes were blocked in blocking buffer (5% non-fat milk ...
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bioRxiv - Microbiology 2019Quote: ... lysed with 100 μg lysozyme and sonication and the filtered cell lysate loaded onto a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... The cells were lysed by sonication and the lysate was separated on a Ni sepharose 6 Fast Flow resin (GE Healthcare) equilibrated with 30 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants from the cell lysate were run on 12% resolving Tris-Glycine gels and then transferred onto PVDF membrane (GE Healthcare). Rabbit α-strep antibody (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell debris was removed by centrifugation and the clear lysate was loaded onto StrepTactin Sepharose High Performance Column (StrepTrap HP, GE Healthcare). The non-specific binding protein and contaminants were flowed through ...
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bioRxiv - Biophysics 2021Quote: ... The cells were then burst open at 19 psi using French press followed by collection of cell lysate and purification using Ni2+-NTA affinity column of AKTATM Pure (GE Healthcare). The proteins were purified using 20 mM imidazole containing binding buffer and 250 mM imidazole containing elution sodium phosphate buffer (pH 7.4) ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins in cell lysates were separated on SDS-PAGE and then transferred electrophoretically onto PVDF membranes (GE Healthcare, Milwaukee, WI, USA) using a transfer cell (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at 16,000xg for 10 min and the clarified lysates were pre-adsorbed to Protein A or Protein G beads (GE Healthcare Bioscience) for 2 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was first purified by Immobilized Metal Affinity Chromatography (IMAC) by loading the clarified lysate onto a 5 mL Nickel column (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed and cleared lysate prepared as described and loaded onto a 5 mL Strep-Trap HP column (GE Healthcare), equilibrated with buffer D ...
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bioRxiv - Plant Biology 2023Quote: ... The ASK1-D3/D3D720K (D3 or D3 with D720K mutation) complex was isolated from the soluble cell lysate by Q Sepharose High Performance resin (GE healthcare). 600 mM NaCl eluates were further subjected to Nickel Sepharose Fast Flow (GE healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on two 5 mL-HiTrap heparin columns (GE Healthcare) at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on a 5 mL-HiTrap heparin column (GE Healthcare) at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... All BtuF constructs were purified directly from whole cell lysate (obtained by sonication) via Ni-NTA chromatography followed by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) in final buffers of 20-50 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Lysate was cleared by centrifugation and the supernatant loaded into an anion exchange column Hi-Trap Sepharose Q-HP (GE Healthcare). KtrA and KtrC were eluted with a 50-1000 mM KCl gradient ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were cleared by centrifugation and the soluble protein fraction was affinity-purified on a HisTrap Excel column (GE Healthcare). After elution in a 0–300 mM imidazole gradient ...
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bioRxiv - Biochemistry 2023Quote: ... Strep-tag purification (used for Rubisco-variants, see SI Table 5): Clarified lysate was applied to a 5 mL StrepTrap column (GE Healthcare) equilibrated with Strep-buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... rPF4 was purified from soluble fractions of cell lysates by affinity chromatography using a 5-ml HiTrap heparin-agarose column (GE Healthcare). Endotoxin-free rPF4 was prepared as previously described 3 ...
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bioRxiv - Cell Biology 2023Quote: ... and pure fractions were pooled and concentrated before being concentrated and snap-frozen in liquid nitrogen GST-tagged RAB7A was similarly isolated via passing cleared lysate over a gravity column packed with 5 mL of Glutathione Sepharose 4B (GE Healthcare), followed by elution using 10 mM reduced glutathione dissolved in 50 mM Tris-HCl pH 8.0. ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Cell Biology 2023Quote: GST fusion protein was similarly purified using affinity chromatography by incubating bacterial cell lysate with glutathione-Sepharose 4B beads (#17075601; GE Healthcare) according to the manufacturer’s instructions and eluted from beads with elution buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... All BtuF constructs were purified directly from whole cell lysate (obtained by sonication) via Ni-NTA chromatography followed by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) in final buffers of 20-50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Lysate was cleared by centrifugation at 20000g for 45 min and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–300 mM in 30 column volumes ...