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Citations for GE Life Sciences :
601 - 650 of 1108 citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The supernatant of the cell lysate was incubated overnight at 4°C on a wheel with Glutathione Sepharose 4B resin (GSTrap 4B, GE Healthcare). After several washes ...
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... rPF4 was purified from soluble fractions of cell lysates by affinity chromatography using a 5-ml HiTrap heparin-agarose column (GE Healthcare). Endotoxin-free rPF4 was prepared as previously described 3 ...
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bioRxiv - Genomics 2023Quote: ... and the lysate were passed through a size exclusion column (Illustra MicroSpin S-400 HR Columns; GE Healthcare, 27-5140-01). RNA > 17 nt was isolated with the RNA Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: ... DSS-treated cell lysates with ultracentrifugation and DSS-treated AcGFP were injected onto a Superdex 200 Increase (10/300GL) column (GE Healthcare), using the UltiMate 3000 UHPLC system (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a pre-washed/pre-equilibrated CM Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL) on an Äkta explorer FPLC (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Microbiology 2024Quote: ... The lysate underwent a subsequent centrifugation at 20,000 □g for 45 □min and the cleared soluble protein lysate was loaded on a prepacked Ni-NTA column (GE Healthcare). Proteins binding to the Ni-NTA coloumns were washed with 20 column volumes of binding buffer supplemented with 20 □mM imidazole to eliminate impurities ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 mM 2-mercaptoethanol for 12 h and purified by anion exchange chromatography (HiTrap Q, GE Healthcare) with a linear gradient of 0 to 500 mM NaCl over 40 min and size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
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bioRxiv - Immunology 2021Quote: ... The lysate was centrifuged to remove cell debris and the supernatant was subjected to affinity chromatography using Ni-NTA beads (GE Life Sciences). The recombinant fusion proteins were eluted in 300 mM Imidazole in PBS pH 7.4while the protein was bound to the column by incubating at 4 °C for 12 to 16 h ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were first pre-cleared for 24 hours at 4°C with G-sepharose beads (GE Healthcare, Chicago, IL, USA) coupled to rabbit IgG (Bethyl Laboratories Inc ...
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bioRxiv - Microbiology 2019Quote: ... FtsZ or ΔCTL were then purified from the lysate using anion exchange chromatography column (HiTrap Q HP 5 ml, GE Life Sciences), followed by ammonium sulfate precipitation (at 20% - 30% ammonium sulfate saturation) ...
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bioRxiv - Biochemistry 2020Quote: ... MALX-E-NirF was purified by loading the cleared cell lysate onto a 5ml MBPtrap HP column (GE Healthcare, Chicago, United States) which was eluted with a gradient to 20 mM maltose ...
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bioRxiv - Immunology 2020Quote: ... Lysates were centrifuged at 14,000 rpm and proteins purified by affinity chromatography using a Ni2+ column (1 mL GE Healthcare HisTrap HP) and imidazole gradient elution in an AKTA Start protein purification system ...
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bioRxiv - Neuroscience 2023Quote: Tissue lysates were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
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bioRxiv - Cell Biology 2024Quote: ... the lysate was clarified by centrifugation and the supernatant was loaded onto either Talon® resin (Clonetech) or glutathione sepharose (GE healthcare) for initial purification ...
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bioRxiv - Cell Biology 2019Quote: ... 2) A Deltavision Elite System (GE Healthcare) equipped with an IX-71 inverted microscope (Olympus) ...
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bioRxiv - Immunology 2019Quote: ... 20U/ml Xuri IL-2 (GE Healthcare) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... with 2% cosmic calf serum (GE Healthcare), 25 ng/mL EGF (R&D ...
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bioRxiv - Molecular Biology 2019Quote: ... cell lysates corresponding to 2×105 cells in the case of in vivo experiments or 4×105 per sample in the case of nuclease-treated lysates were analyzed by electrophoresis on 10 or 12% SDS-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science). The proteins of interest were probed with antibodies against the following proteins ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was adjusted to 35mM imidazole and loaded onto a 5ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) equilibrated in 20mM HEPES pH 7.4 ...
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bioRxiv - Plant Biology 2019Quote: ... centrifuged at 17,500 RPM for 60 minutes and the clarified lysate loaded onto a 5 mL HisTrap HP (GE Healthcare, Chicago, IL, USA). The HisTrap HP column was washed with wash buffer (25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2019Quote: ... centrifuged at 17,500 RPM for 60 minutes and the clarified lysate loaded onto a 5 mL GSTrap HP (GE Healthcare, Chicago, IL, USA). The GSTrap HP column was washed with wash buffer (25 mM Tris (pH 8.0) ...
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bioRxiv - Developmental Biology 2019Quote: ... 30 μg of proteins from lysates were subjected to migration on 8–12 % acrylamide gels and transferred on to polyvinylidene difluoride membranes (GE Healthcare Europe GmbH) in borate buffer (50 mM Tris-HCl and 50 mM borate ...
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bioRxiv - Biophysics 2019Quote: ... for 20 min at 4 0C and histidine tagged protein was recovered from the lysate using a HiTrap TALON Crude Co2+ column (GE Healthcare, Piscataway, NJ) as described by the manufacturer ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... and purified the MTBD constructs from the lysates using a HisTrap High Performance nickel Sepharose column (17-5247-01, Cytiva (formerly GE Healthcare Life Sciences), Marlborough ...
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bioRxiv - Neuroscience 2020Quote: ... and 25μg of whole protein lysate were resolved on SDS-PAGE gels prior to transfer to PVDF membranes (GE Healthcare Life Sciences, #10600023) using a tank blotting apparatus (BioRad).
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were immunoprecipitated with the indicated antibodies for 3 h at 4 °C and then incubated with protein G (GE Healthcare Life Sciences) for 2 hrs at 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... The cells were lysed using an MSE Soniprep 150 (Wolf Laboratories, York, UK) and the cell-free lysate was applied to a 5 mL HisTrap HP column (GE Healthcare, Amersham, UK) and PL1 was eluted using a 5–500 mM imidazole gradient ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified cell lysate produced as for D2-I and FANCI was incubated with Strep-Tactin Sepharose High Performance resin (GE Healthcare Life Sciences) for 60 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... The lysate was then subjected to nickel-immobilized metal affinity chromatography (IMAC) using a HisPrep™ Fast Flow 16/10 column (GE Healthcare, USA) equilibrated with equilibration buffer (20 mM NaH2PO4 ...
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bioRxiv - Microbiology 2021Quote: Whole cells lysate (50 µg) was separated on 12% SDS-PAGE and then transferred onto a PVDF membrane (GE Healthcare, Piscataway, NJ, USA). Membrane were blocked in 5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... and 25µg of whole protein lysate were resolved on SDS-PAGE gels prior to transfer to PVDF membranes (GE Healthcare Life Sciences, #10600023) using a tank blotting apparatus (BioRad).
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bioRxiv - Immunology 2022Quote: ... and the resultant lysate was incubated overnight at 4 °C with 1.5 ml of glutathione-Sepharose 4B bead slurry (GE Healthcare, 17-0756-01). Beads were washed three times in wash buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... were purified from bacterial lysate by affinity chromatography on a GSTrap FF column (Cat. No. 71-5016-96 AM, GE Healthcare, Chicago, IL) followed by dialysis against multiple changes of phosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM L-Glutamine (GE Life Sciences #SH30852.01), 100 units/mL Penicillin and 100 μg/mL Streptomycin (Corning #30-001-CI ...
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bioRxiv - Cell Biology 2021Quote: ... GST-hEBP50 in pGEX-6P-2 (GE Healthcare): GST-hEBP50/WT (AA95-168) ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM βME using Glutathione Sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM βME using glutathione sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
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bioRxiv - Cancer Biology 2022Quote: ... siRNA non-targeting pool #2 (GE Healthcare Dharmacon) was used as a control for all the knockdown experiments ...
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bioRxiv - Neuroscience 2020Quote: ... the 2-D Clean-Up Kit (GE Healthcare) was used following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... (2) Ni Sepharose fast flow resin (GE Healthcare) chromatography using a step elution from 50 mM to 200 mM Imidazol in Tris buffer B (20 mM Tris/HCl ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM βME using glutathione sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
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bioRxiv - Microbiology 2023Quote: ... 2 µl of either anti-GST (GE Healthcare), anti-HA (Sigma H6908) ...
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bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare, Vélizy-Villacoublay, France), 0.5% penicillin-streptomycin (Invitrogen)) ...
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bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... Whole cell lysates were subjected to SDS–PAGE and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before[30] ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...