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Citations for GE Life Sciences :
451 - 500 of 1108 citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The recombinant protein in the supernatant of the cell lysate was mixed with Glutathione Sepharose resin (GE Healthcare) and the mixture was washed extensively in 10mM HEPES pH 7.3 ...
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bioRxiv - Cell Biology 2022Quote: ... Clear lysate was incubated with 0.5 ml packed beads IgG Sepharose 6 Fast-Flow (GE Healthcare, Chicago, IL) for 2-3 hours at 4°C on a tube roller ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated with mouse monoclonal anti-MYC-antibody (Covance) and protein G-agarose beads (GE Healthcare). The precipitates were then washed with a lysis buffer ...
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bioRxiv - Physiology 2023Quote: ... The vector was purified from the clarified lysate by affinity chromatography using a HiTrap heparin column (GE Healthcare), ultracentrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Bioengineering 2023Quote: ... The ErCas12a protein in clarified lysate was sequentially purified using immobilized metal affinity chromatography (HisTrap HP, GE Healthcare) and heparin chromatography (HiTrap Heparin HP ...
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bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Filtered lysate was applied to a 1-ml His-Trap (all FPLC columns were obtained from GE Healthcare) column using an ÄKTA FPLC ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was cleared by centrifugation and loaded on a Ni-NTA agarose column (HisTrap HP; GE Healthcare). After rigorous washing with 100 mM Tris-HCl pH 8.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Lysate samples were separated by SDS-PAGE or LDS-NuPAGE and transferred to 0.2μm nitrocellulose membrane (GE Healthcare) via tank immersion ...
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bioRxiv - Neuroscience 2020Quote: ... pGEX-4T-2 (GE Healthcare), pGIPZ control shRNA ...
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bioRxiv - Plant Biology 2021Quote: ... 2 M thiourea (GE Healthcare), 1% DTT ...
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bioRxiv - Plant Biology 2020Quote: ... pGEX6p-2 (GE healthcare, IL) and pBE2113-GFP (Yoshida et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare), 0.5% penicillin-streptomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was cleared with centrifugation and bound to Glutathione sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-02). Beads were washed several times with lysis buffer and protein was eluted from beads with 10 mM reduced glutathione in lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was incubated with Glutathione Sepharose beads (20 μL for one group, 17-0756-01, GE healthcare, Sweden) at 4 °C overnight ...
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bioRxiv - Plant Biology 2019Quote: ... the clarified lysate was applied to a Ni2+-NTA column connected to an AKTA Xpress purification system (GE Healthcare). Proteins were step-eluted with elution buffer (50 mM Tris-HCl pH7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... XPG and XPA containing lysate was applied onto GE HisTrap HP 5 mL (GE Healthcare, Little Chalfont, United Kingdom) equilibrated in lysis buffer (in case of XPA all downstream steps were performed in the presence of 5 mM β-mercaptoethanol and 10 μM ZnCl2 instead of 1 mM DTT) ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysate was pre-incubated with 200 μL of rehydrated Protein A Sepharose CL-4B beads (GE Healthcare, Life Sciences) for 15 minutes at 4°C with rocking ...
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bioRxiv - Biophysics 2019Quote: ... Lysates were cleared by centrifugation (10000 rpm) and incubated with Glutathione Sepharose 4B beads (GE Life Sciences, Freiburg, Germany) on a rotating platform at 4°C for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were centrifuged and the supernatants used for the purification on HisTrap HP columns (1 mL; GE Healthcare) connected to an AKTA Explorer system (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... The lysate was centrifuged and the clarified supernatant loaded onto a 5ml HisTrap HP nickel affinity column (GE Healthcare). dCry protein was eluted with 100 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: The filtered lysates were subjected to IMAC using a His-Trap 5 mL Nickel column (GE Healthcare Life Sciences) on an ÄKTA Pure system (GE Healthcare Life Sciences) ...
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bioRxiv - Plant Biology 2020Quote: ... the clarified lysate was applied to a Ni2+-NTA column connected to an AKTA Xpress purification system (GE Healthcare). Proteins were step-eluted with elution buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was clarified by centrifugation at 40,000 rpm for 45 minutes and loaded onto a HisTrap column (GE Healthcare) equilibrated in nickel buffer A (50 mM HEPES (pH 7.6) ...
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bioRxiv - Cancer Biology 2021Quote: ... The soluble fraction of the cell lysate was purified on HiTrap IMAC HP columns (GE Healthcare # 17-0920-05) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... All protein lysates were run on NuPage 4–12% gradient gels (LifeTechnology) and transferred to PVDF membrane (GE Healthcare). Membranes were blocked in 5% milk in TBST and stained with primary antibody overnight at +4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were diluted with PBS to 10 ml and incubated with 200 μl of GST-Sepharose (GE Healthcare) for 1 hour on ice under agitation ...
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bioRxiv - Microbiology 2021Quote: ... and the filtered lysate was passed sequentially through HisTrap Excel nickel and HiTrap CP cation exchange columns (GE Healthcare) to purify the tagged TOP2α protein before incubation overnight with His-tagged TEV protease ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified cell lysate produced as for D2-I was loaded onto a HisTrap HP column (GE Healthcare Life Sciences). Using an imidazole gradient ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysates containing Hb were initially purified using a Zn2+ charged chelating Sepharose Fast Flow resin column (GE healthcare) because Hb surfaces are rich with His residues that can bind Zn2+ (also see (42)) ...
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bioRxiv - Biophysics 2019Quote: ... Clarified cell lysate containing His6-tagged protein was loaded onto a Ni–Sepharose affinity column (HisTrap HP; GE Healthcare). The column was washed with wash buffer (5% glycerol ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate is incubated with 100ul of equilibrated IgG slurry (IgG SepharoseTM 6 Fast Flow from GE Healthcare) overnight in rotator wheel at 4°C with slow rotation ...
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bioRxiv - Biophysics 2020Quote: ... The protein was purified from the supernatant of the lysate using a 5 ml HisTrap HP column (GE Healthcare). The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... In order to purify MIF the lysate was first passed over a 5-mL CM-sepharose column (GE Healthcare), and after washing with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was cleared by centrifugation and SUMO-tagged IMPDH chromatographically purified with HisTrap FF columns (GE Healthcare Life Sciences) and an Äkta Start chromatography system ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was filtered (0.45 µm) and then loaded onto a 5 ml HisTrap FF Crude column (GE Healthcare) equilibrated with wash buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were clarified by centrifugation and incubated with 20 mM imidazole on Ni Sepharose High Performance Resin (GE Healthcare) at 4°C for 4 hours ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was pelleted and cell lysate was filtered with a 0.2μm filter and loaded onto a HisTrap Ni column (GE Healthcare) at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was filtered (0.8 μM) and applied to a 10 or 20 ml HisTrap FF (GE Healthcare) equilibrated in washing buffer (lysis buffer without protease inhibitors) ...
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bioRxiv - Plant Biology 2022Quote: ... the supernatant lysate was applied to a Ni2+-NTA column connected to an AKTA Xpress purification system (GE Healthcare) (Maqbool et al ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lysate was then 0.45 μm filtered and applied to a 5 mL HisTrap HP column (GE Healthcare). The column was washed with five column volumes of wash buffer ...
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bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superose 6 Increase 10/300 GL column (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was incubated for 45 minutes with 20 µl of Ni Sepharose 6 Fast Flow beads (GE Healthcare), washed and resuspended in 100 µl of buffer A (30 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: Protein lysates were separated on 10% SDS-PAGE gels and then transferred to PVDF membranes (GE Healthcare Life Science). The membranes were blocked in phosphate-buffered saline with Tween 20 (PBST ...
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bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 1 h at 95,000g and the supernatant was loaded onto a Glutathione Sepharose HP (GE Healthcare) column ...
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bioRxiv - Biochemistry 2019Quote: ... into the periplasm and purified from the periplasmic lysate via anion exchange chromatography (Q-sepharose, GE Life Sciences Cat: 25236), using 20 mM Tris-HCl pH 8.0 with 20 mM NaCl (Buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... proteins (10 μL each of lysate or wall fractions) were separated in a 12% polyacrylamide gel before transfer to 0.2 μm nitrocellulose (GE Healthcare) for detection by western blotting ...