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Citations for GE Life Sciences :
3401 - 3450 of 4188 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... insoluble material was removed by centrifugation at 10,000 × g and the cell lysate was pre- cleared by addition of heat inactivated goat serum and protein G sepharose beads (GE Healthcare). Proteins were then either immunoprecipitated by adding 2 μg mouse anti-GFP (Clones 7.1 and 13.1 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was extracted and from cell monolayers using a magnetic bead-based protocol using Sera-Mag SpeedBeads™ (GE Healthcare). Isolated RNA was DNase-treated with RQ1 DNase (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant of the cell lysate was incubated overnight at 4°C on a wheel with Glutathione Sepharose 4B resin (GSTrap 4B, GE Healthcare). After several washes ...
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bioRxiv - Molecular Biology 2023Quote: ... and imaged on a Nikon Eclipse Ti2 at 60X or 100X objective with oil immersion or at 20X using In Cell Analyzer 6000 microscope (GE Healthcare). Images were captured and analyzed using Elements software (v 5.41.02) ...
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bioRxiv - Biochemistry 2024Quote: ... The pooled fraction was concentrated on an Amicon 10kDa MWCO cell and applied to a sephacryl S300 HR column (GE Healthcare) equilibrated with Buffer F (25mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... DSS-treated cell lysates with ultracentrifugation and DSS-treated AcGFP were injected onto a Superdex 200 Increase (10/300GL) column (GE Healthcare), using the UltiMate 3000 UHPLC system (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) from fresh peripheral blood of healthy donors were isolated by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare). Cytotoxic T cells and T helper cells were isolated from the PBMCs by positive selection with CD8- and CD4-specific MicroBeads (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2024Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from heparin-treated whole blood by density gradient centrifugation (Ficoll-Paque PREMIUM, GE Healthcare). After separation ...
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bioRxiv - Microbiology 2023Quote: ... from 3 different healthy donors to avoid unique donor bias on type cells effects and extracted using Ficoll-Plaque Plus solution (GE Healthcare). PBMCs were re-suspended in RPMI-1640 medium (Lonza Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... Gp140 proteins were purified from clarified cell culture supernatants 4 days post-transfection using Galanthus nivalis lectin (GNL) affinity chromatography followed by a Q-sepharose (GE Healthcare) polishing step to remove host cell protein contaminants ...
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bioRxiv - Microbiology 2024Quote: Peripheral blood mononuclear cells (PBMCs) were recovered from heparinized blood via density gradient centrifugation using Ficoll-Plaque separation media (GE Healthcare) and Leucosep tubes (Greiner Bio-One) ...
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bioRxiv - Neuroscience 2024Quote: Quantitative analysis of the sagittal sections was performed using the automated microscope IN Cell Analyzer 6000 (GE Healthcare, Chicago, IL, USA). Four microscope slides were loaded into the system ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by visualization of the Ypet signal intensity and nuclear localization by live imaging using the YFP channel on a IN Cell Analyzer 2200 apparatus (GE healthcare). One homozygous clone of SYA cells was then transduced with a lentiviral vector containing EF1a-OsTir1_SNAP-P2A-puro and selected with 2 μg/ml of Puromycin (Gibco A11138-03 ...
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bioRxiv - Molecular Biology 2024Quote: ... Fly-FUCCI wing discs and transfected Kc cells were acquired using a 60x oil immersion objective (NA 1.42) on a DeltaVision microscope (DeltaVision Spectris; Applied Precision, LLC). For all DSB tracking experiments in wing discs ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was collected and incubated for 5 min on 1 mL Ni Sepharose® 6Fast Flow (GE Healthcare) equilibrated with 10 mL binding buffer ...
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bioRxiv - Biophysics 2021Quote: ... 10 μM ZnCl2 and 1 mM DTT) and loaded into a HiTrap Heparin HP 5 ml column (GE Healthcare) equilibrated with 6.25% of buffer HepB (buffer HepA supplemented with 2 M NaCl) ...
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bioRxiv - Biophysics 2019Quote: ... 50 mM KAc,1 mM DTT) Further purification was performed using size exclusion chromatography (Superose-6 increase, GE Healthcare) using Shimadzu High Performance Liquid Chromatograph ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM TCEP] by size-exclusion chromatography using a HiLoad 16/60 Superdex 200 gel-filtration column (GE Healthcare). PelXC232S eluted as a single Gaussian shaped peak ...
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bioRxiv - Cell Biology 2020Quote: ... In a 48 well plate, 25-32.5μL siRNA (for sequences, see table 1) was mixed with 25uL 1x siRNA buffer (GE Healthcare) containing 0.5uL Dharmafect 1 transfection reagent ...
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bioRxiv - Developmental Biology 2021Quote: ... For monoclonal antibody production, the DNA encoding mouse KCTD19 (residue 654-793 aa, NP_808459.1) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and fractions contacting samples were loaded onto size-exclusion column (Superdex 75 16/60, GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... Three washes were performed with TBST followed by 1 h incubation with secondary antibody (ECL, HRP linked, GE Healthcare) and another series of washes ...
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bioRxiv - Microbiology 2019Quote: ... membranes were incubated for 1 hour with secondary α-rabbit antibodies conjugated with horseradish peroxidase (HRP) (GE healthcare, NA934) diluted by 1:10,000 in 5% skim milk in TBST ...
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bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were washed with TBS-T and incubated with HRP secondary antibodies (GE Healthcare Life Sciences, 1:2000), and immunobands were detected with a Pierce ECL Western Blotting Substrate (Thermo Fischer Scientific ...
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bioRxiv - Physiology 2019Quote: ... diluted 1:5,000 and developed using Amersham ECL Western Blotting Detection Kit system (GE Healthcare Life Sciences, PA, USA).
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies used were horseradish peroxidase (HRP)-conjugated anti-mouse antibody (sheep polyclonal, used at 1:3000, GE Healthcare), HRP-conjugated anti-rabbit antibody (sheep polyclonal ...
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bioRxiv - Genetics 2020Quote: ... Eluted fractions were diluted 6.5-fold with R buffer and loaded onto a 1 mL ResourceQ column (GE Healthcare). Rrp1 eluted at around 300 mM NaCl with a linear gradient of 0.1-1.0 M NaCl in R buffer ...
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bioRxiv - Genetics 2020Quote: ... then washed for 3 × 20 min in 1× SSC and 0.1% SDS and imaged using a phosphorimager (GE Healthcare) and scanned using an Amersham Typhoon 5 scanner ...
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bioRxiv - Neuroscience 2020Quote: ... before they were incubated with HRP-conjugated secondary antibodies (1:10000, NXA931 and NA934, GE Healthcare Europe, Freiburg, DE) also diluted in blocking solution for 1 h at RT ...
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bioRxiv - Synthetic Biology 2021Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with two different 32P-radiolabeled probes (Church and Gilbert ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactivity was detected using species-appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (1:5000; GE Healthcare, Cleveland, OH, USA). Western blots were imaged via HRP chemiluminescent detection (Azure Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were centrifuged and the supernatants used for the purification on HisTrap HP columns (1 mL; GE Healthcare) connected to an AKTA Explorer system (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 mM DTT and loaded directly onto a 5 ml HiTrap SP Sepharose fast flow column (GE Healthcare). Bound Nup84-Nup133 was eluted from the column using a 15 column volume (CV ...
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bioRxiv - Biochemistry 2020Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Biochemistry 2020Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM Tris pH 7.2 ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... An enhanced chemiluminescence (ECL)-horse radish peroxidase (HRP) conjugated secondary antibody (NA934V, 1:1000 dilution, GE Healthcare, Uppsala, Sweden) and SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL, GE Healthcare, Chicago, IL). Polyclonal affinity purified NS3 and NS5 proteins were produced as previously described (20) ...
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bioRxiv - Immunology 2021Quote: ... IgGs were purified from ~1 ml of plasma with equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted from the resin with 0.1 M glycine at pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... MERS-CoV NTD and SARS-CoV-1 NTD were then further purified using a Superdex 75 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Chromosomal DNA fragments were separated on a 1% agarose gel and blotted onto Hybond N+ nylon membranes (GE Healthcare), following standard techniques (Sambrook and Russell ...
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bioRxiv - Biophysics 2021Quote: ... and MtCNNMCorC (residues 333-417) were subcloned into BamHI and XhoI sites of pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST-tag.
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bioRxiv - Biophysics 2020Quote: ... 50 mM KAc,1 mM DTT) Further purification was performed using size exclusion chromatography (Superose-6 increase, GE Healthcare) using Shimadzu High Performance Liquid Chromatograph ...
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bioRxiv - Biophysics 2020Quote: ... Protein fractions (eluted at ~ 1 M NaCl) were then purified using a HiLoad 16/60 Superdex 75 (GE Healthcare) column (SEC ...
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bioRxiv - Biochemistry 2022Quote: ... 100 µL TIR-SAVED (10.9 mg.mL-1) were injected into the Superose 12 column 10/300 GL (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 mM DTT] and filtered on a Superdex 200 10/300 GL column (GE Healthcare, Little Chalfont, UK). Aliquots of the protein were flash-frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity was detected using a horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (IgG, 1:10,000, NA934 GE Healthcare). Tau13 and Tau5 immunoreactivity were detected using an anti-mouse IgG (1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... Ten μl of each culture were spotted onto 15 mm grade 1 Whatman filter discs (GE Healthcare Life Sciences). After drying for 1 minute at room temperature ...
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bioRxiv - Biochemistry 2021Quote: A sample of nanodiscs reconstituted LicB:sybody-C complex was concentrated between 1 to 1.5 mg/ml using a Vivaspin concentrator with a 30,000 Da cutoff (GE Healthcare). Cryogenic samples were prepared using a Mark IV Vitrobot (Thermo Fisher ...