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Citations for GE Life Sciences :
3201 - 3250 of 4188 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Immune cells were enriched through incubation with ACK buffer (spleen) or through density gradient centrifugation using Ficoll-Paque (GE Healthcare) following the manufacturer’s protocol (blood and liver) ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood through Ficoll density centrifugation (Ficoll-Plaque PLUS, GE Healthcare, USA), aliquoted and stored in liquid nitrogen ...
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bioRxiv - Immunology 2020Quote: ... mononuclear cells were isolated from buffy coats blood using Ficoll-Paque™ PLUS (γ=1.007, GE Healthcare, Chicago, IL, USA). Next ...
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bioRxiv - Cell Biology 2021Quote: Procyclic (PCF) cell line Tb427 29.13 Trypanosoma brucei brucei (86) was grown in SDM-79 medium (GE Healthcare, G3344-3005) pH7.4 supplemented with 2 mg/mL hemin (Sigma Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... After loading the cell lysate onto a Protein L beads (Capto™ L, GE Healthcare, Cat. no. 17-5478-02) column ...
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bioRxiv - Cancer Biology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were separated by density gradient centrifugation using Ficoll-Paque Premium (#17-5442-02; GE Healthcare). PBMCs were washed 3x with PBS ...
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bioRxiv - Genetics 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated in two hours by using Ficoll Paque Plus (17-1440-02, GE Healthcare), deposited in RNAlater (Am7021#RNAlater Soln ...
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bioRxiv - Microbiology 2022Quote: Cell densities were determined based on the optical density at 600 nm (OD600) using an Ultraspec 2100 spectrophotometer (GE Healthcare) or a Tecan Infinite M1000 microplate reader ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Germany) and exchanged to the desired cell culture medium using Sephadex G-25 PD10 desalting columns (GE Healthcare Bio-Science). The final concentration and integrity of eGFP-WNT-3A in the conditioned medium were determined using both ELISA (GFP ELISA® kit ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with cold phenol red-free DMEM with 10% FBS and 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses and incubated at 37 °C to initiate virus-cell fusion ...
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bioRxiv - Microbiology 2023Quote: ... the cell supernatant was collected and soluble IgG was purified using affinity chromatography (GammaBind Plus Sepharose, GE HealthCare, Chicago, IL). Purified IgG was buffer-exchanged in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Live imaging of GFP or RFP immunofluorescence in COs and NICOs was obtained using IN Cell Analyzer 2200 (GE Healthcare) at 4x or 10x magnification ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation on lymphocyte separation medium (LSM 1077, GE Healthcare) as previously described191 ...
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bioRxiv - Immunology 2024Quote: ... Liver infiltrating cells were obtained after 25 min centrifugation (600 g without brake) in a 35% and 67.5% bilayer Percoll (GE Healthcare) gradient ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation on Ficoll-Paque Plus (GE Healthcare, Boston, MA, USA). CD3+ T cells from PBMCs were separated using CD3 microbeads (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from plated cells or snap-frozen kidneys using the RNAspin Mini kit (GE Healthcare, #25-0500-72) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Images of mouse spermatocyte spreads testicular cell squashes were acquired using a computer-assisted fluorescence microscope system (DeltaVision; Applied Precision) with a 60ξ NA 1.4 oil immersion objective ...
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were isolated from the cell suspension using a 37 % and 70 % percoll gradient (GE Healthcare Bio-Sciences AB, Sweden) diluted with HBSS (with or without phenol red ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant containing the cells was filtered through 70-μm strainers into a tube containing 90% isotonic Percoll (GE Healthcare, #17-0891-01 ...
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bioRxiv - Molecular Biology 2023Quote: The supernatant or cell extract was then filtered through a 0.45 µm pore filter and loaded on a HisTrap HP column (GE Healthcare) with immobilized Ni2+ beads equilibrated with HisBufferA (50 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood samples by separation on a Ficoll-Hypaque gradient (GE Healthcare). For detailed splenocyte isolation protocol ...
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bioRxiv - Biophysics 2023Quote: YidC and YidCΔC were purified from pTrc99a-YidC (Welte et al., 2012) and pTrc99a-YidCΔC expressing BL21 cells using a Ni-NTA FF crude column (GE Healthcare) on an ÄKTA chromatography system ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then imaged at 4X using the InCell 2200 or 6500 automated fluorescence microscope system (GE Healthcare Life Sciences) and quantitative analysis was performed with Cell Profiler Software(182,183) ...
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bioRxiv - Immunology 2023Quote: ... from which the debris and the dead cells were removed by density gradient centrifugation with a 46% Percoll (GE Healthcare) gradient ...
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bioRxiv - Genomics 2023Quote: ... HepG2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Hyclone, GE healthcare) and 1% Penicillin-Streptomycin (PS ...
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bioRxiv - Bioengineering 2024Quote: ... the soluble fraction of the cell lysate was passed through a 0.45-micron filter and then loaded onto a HisTrap HP 5 ml (GE Healthcare) column equilibrated with buffer A (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using an AKTA FPLC with 1 mL nickel-charged HisTrap columns (GE Healthcare Life Sciences) for affinity chromatography of the His-tagged proteins ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies were incubated for 1 hour at RT and signals were detected with ECL Select (GE Healthcare) and Odyssey Fc (Li-Cor) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Neuroscience 2021Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... it was passed through Whatman™ Grade 1 filter paper (diameter 150 mm) (GE Healthcare Life Sciences, Ireland), followed by 0.45-μm membrane filtration and then 0.22-μm membrane filtration (ThermoFisher Scientific Ltd) ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was probed with HRP-conjugated anti-mouse or anti-rabbit (1:5,000, GE Healthcare Bio-Sciences) secondary antibody after washing several times with 1X PBS for 45 min at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Biochemistry 2019Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Biochemistry 2019Quote: ... Concentrated DJ-1/Hsp32 was loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare), pre-equilibrated with 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were solubilized in 1% (w/v) LMNG and proteins were purified using a StrepTrap column (GE Healthcare). The resin was washed in TBS containing 0.01% (w/v ...
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bioRxiv - Genetics 2019Quote: ... Primary antibodies were detected by HRP-conjugated anti-rabbit IgG (GE Healthcare NA934V, Lot 9636020; 1:10000 dilution) or HRP-conjugated anti-mouse IgG (GE Healthcare NA931V ...
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bioRxiv - Microbiology 2021Quote: ... The amplified wild type and mutant PDE12 cDNAs were cloned into plasmid pGEX-6P-1 (GE Healthcare, USA) at the EcoRI and SalI restriction site ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatants were collected by centrifugation and purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... GST-TEV-FICD was then depleted by a 1 h incubation with GSH-Sepharose 4B matrix (GE Healthcare). AMPylation was confirmed as being stoichiometric by intact-protein mass spectrometry (LC-ESI-MS ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μL of plasma was loaded onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, USA) and left to dry out ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was transferred on a column containing 1 ml Ni-Sepharose High Performance resin (GE Healthcare), washed two times with lysis buffer containing 20 mM and 40 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT with USP7 proteins purified as described previously 62 using Glutathione Sepharose 4B beads (GE Healthcare). After elution the GST tag was removed under dialysis using 3C protease and the sample fractionated by PorosXQ anion exchange (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Biophysics 2020Quote: ... excess FluoZin-1 dye was removed by passing the sample through a PD-10 desalting column (GE Healthcare) pre-equilibrated with reconstitution buffer.
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies were diluted in blocking solution at 1:1000 (Anti-rabbit IgG: GE Healthcare Cat#NA934, RRID:AB_772206) and 1:2000 (Anti-mouse IgG ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D534 was also purified using 1 mL of M2 anti-FLAG affinity column (GE healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: The HMWP2-Cy2 supernatant was loaded at 1 ml/ min onto a 5 mL HisTrap column (GE Healthcare) pre-equilibrated with lysis buffer ...