Labshake search
Citations for GE Life Sciences :
3351 - 3400 of 4188 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2020Quote: ... Whole blood was diluted with room temperature RPMI medium in a 1:2 ratio to facilitate cell separation for other analyses using the SepMate PBMC isolation tubes (STEMCELL) containing 16 mL Ficoll (GE Healthcare). Diluted whole blood was centrifuged at 1200 g for 20 minutes at 20 °C ...
-
bioRxiv - Molecular Biology 2020Quote: Whole cell lysates were extracted from cells in 2x sample buffer and separated on 10% SDS– PAGE gel and transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with Odyssey Blocking Buffer in PBS (Licor) ...
-
bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation using Ficoll-Paque™ Plus (GE Healthcare Europe, Brøndby, Denmark). They were either examined directly ex vivo or cryopreserved in 10% DMSO and 90% FCS at -150°C for later in vitro analysis.
-
bioRxiv - Cell Biology 2019Quote: ... as capture molecule to the dextran surface of both flow cells of a CM5 Chip (GE Heathcare) using an amine coupling kit (GE healthcare). GST tagged ligand proteins were captured on the detection flow cell of the chip and free GST was captured on the reference flow cell ...
-
bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
-
bioRxiv - Physiology 2021Quote: ... The hemolysate was then centrifuged at 4°C (12000 g, 20 min) to remove cell debris and then passed (‘stripped’) on a PD-10 desalting column (GE Healthcare) equilibrated with 10 mM HEPES ...
-
bioRxiv - Microbiology 2020Quote: ... Cell debris was pelleted at 15000 × g for 10 min and 10 μg of the cleared cell lysates were resolved on a 10% SDS-PAGE and transferred to 0.45 μm nitrocellulose membrane (GE life Sciences). Membranes were blocked in blocking buffer (5% non-fat milk ...
-
bioRxiv - Microbiology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood within 24 hours of sample collection using Ficoll-Paque (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Zoology 2020Quote: ... Proteins were separated in the second dimension on a 14% SDS polyacrylamide gel (Laemmli, 1970) in a Hoefer600 cell (GE Healthcare) for 15 min at 15 mA (100 V/15 W limits ...
-
bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats by density gradient centrifugation on Ficoll-Paque™ PLUS (1.077 g/L; GE healthcare). Then ...
-
bioRxiv - Biochemistry 2019Quote: ... Cell debris was pelleted by centrifugation and the soluble fraction was loaded onto a HisTrap-HP Ni-Sepharose column (GE Healthcare). Bound protein was eluted with lysis buffer supplemented with 300 mM imidazole ...
-
bioRxiv - Cell Biology 2019Quote: ... human adult peripheral blood was harvested from healthy donors after informed consent and immune cell isolated using Ficoll-Paque gradient (GE Healthcare). Buffy coat was harvested for PBMCs while granulocytes were isolated from the gradient pellet ...
-
bioRxiv - Biophysics 2019Quote: ... The cells were lysed by sonication and the lysate was separated on a Ni sepharose 6 Fast Flow resin (GE Healthcare) equilibrated with 30 mM Tris-HCl pH 8 ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were expressed transiently in HEK-293T cells and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare) or streptactin beads (IBA ...
-
bioRxiv - Genomics 2021Quote: ... followed by IgG-affinity chromatography and finally anion-exchange using a previously described procedure (Moreno-Morcillo et al., 2014) with the following modifications: for cells lysis and equilibration of the heparin column (GE Healthcare), we used instead a buffer containing 250 mM Tris–HCl pH 8 ...
-
bioRxiv - Microbiology 2020Quote: ... Supernatants from the cell lysate were run on 12% resolving Tris-Glycine gels and then transferred onto PVDF membrane (GE Healthcare). Rabbit α-strep antibody (1:1000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Hippocampal neurons cultured for 14 days and immunostained for cofilin were imaged in an automated fluorescence widefield high-content screening microscope (IN Cell Analyzer 2000, GE Healthcare) at 40x magnification ...
-
bioRxiv - Neuroscience 2021Quote: Brain sections stained for cofilin were imaged in an automated fluorescence widefield high-content screening microscope (IN Cell Analyzer 2000, GE Healthcare) at 20x magnification ...
-
bioRxiv - Biophysics 2021Quote: ... The cells were then burst open at 19 psi using French press followed by collection of cell lysate and purification using Ni2+-NTA affinity column of AKTATM Pure (GE Healthcare). The proteins were purified using 20 mM imidazole containing binding buffer and 250 mM imidazole containing elution sodium phosphate buffer (pH 7.4) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The number of nuclei was determined using high-content analysis with the IN Cell Analyzer automated microscope (GE Healthcare, Reading, UK). Data are reported as relative cell numbers in % of the positive control.
-
bioRxiv - Cell Biology 2020Quote: Secreted WNTs were enriched from cell culture supernatants using the affinity chromatography resin Blue Sepharose 6 Fast Flow (17-0948-01, GE Healthcare) and analysed by Western blotting according to previously published protocols (Glaeser et al. ...
-
bioRxiv - Immunology 2020Quote: ... Neutrophils (purity > 97%, viability > 98%) were isolated from healthy heparinised peripheral blood using HetaSep (Stem Cell) and Ficoll-Paque (GE Healthcare), and contaminating erythrocytes were removed by hypotonic lysis as described previously (Wright et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins in cell lysates were separated on SDS-PAGE and then transferred electrophoretically onto PVDF membranes (GE Healthcare, Milwaukee, WI, USA) using a transfer cell (Bio-Rad ...
-
bioRxiv - Immunology 2021Quote: ... Protein was purified from filtered cell culture supernatants by StrepTactin resin (IBA) and by size exclusion chromatography using Superose 6 column (GE Healthcare) in 10 mM Tris pH8,150 mM NaCl or 2 mM Tris pH 8 ...
-
bioRxiv - Molecular Biology 2020Quote: The distance between two GFP foci on chromosome IV on a single focal plane in a intact yeast cell taken by the fluorescence microscope system (Delta vision, Applied Precision) was measured by NIH image J program.
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed and incubated with DAPI and anti-bleach reagents prior to imaging on a DeltaVision Elite Microscope (GE Healthcare). For all experiments ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysates from 8 L of yeast treated cells were then subjected to a 5 ml Nickel affinity purification using an AKTA purifier system (GE Healthcare) equilibrated in the urea buffer ...
-
bioRxiv - Immunology 2022Quote: ... Umbilical cord blood mononuclear cells (UCBMC) and plasma were obtained by standard density gradient centrifugation over Ficoll (GE Healthcare, Chicago, IL). UCBMC were frozen in 10% DMSO/FetalPlex (GeminiBio ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were lysed and cleared lysate prepared as described and loaded onto a 5 mL Strep-Trap HP column (GE Healthcare), equilibrated with buffer D ...
-
bioRxiv - Plant Biology 2023Quote: ... The ASK1-D3/D3D720K (D3 or D3 with D720K mutation) complex was isolated from the soluble cell lysate by Q Sepharose High Performance resin (GE healthcare). 600 mM NaCl eluates were further subjected to Nickel Sepharose Fast Flow (GE healthcare ...
-
bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
-
bioRxiv - Biophysics 2022Quote: ... Broken cells then underwent ultra-centrifugation at 142,000 x g for 60 minutes before collecting the cell free extract and incubating it with pre-equilibrated HisTrap Ni2+ resin (GE Healthcare) in a 3:1 volume ratio for 2 hours under gentle shaking ...
-
bioRxiv - Cancer Biology 2022Quote: ... U87vIII CHCHD2 WT and KO cells were seeded onto collagen-coated dextran beads (∼200 µm diameter, GE Life Sciences, Pittsburgh, PA) at a density of 2 × 106 cells per 5 × 103 beads in 5 mL DMEM ...
-
bioRxiv - Microbiology 2024Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from heparin-treated whole blood by density gradient centrifugation (Ficoll-Paque PREMIUM, GE Healthcare). After separation ...
-
bioRxiv - Biochemistry 2024Quote: ... The pooled fraction was concentrated on an Amicon 10kDa MWCO cell and applied to a sephacryl S300 HR column (GE Healthcare) equilibrated with Buffer F (25mM Tris pH 7.5 ...
-
bioRxiv - Cancer Biology 2024Quote: ... 60 μl of this mixture was added in the HUVEC side channel of the chip and max intensity projections were taken every 5 minutes for 30 minutes using the IN Cell Analyzer 6000 (GE Healthcare) microscope.
-
bioRxiv - Microbiology 2023Quote: ... Gp140 proteins were purified from clarified cell culture supernatants 4 days post-transfection using Galanthus nivalis lectin (GNL) affinity chromatography followed by a Q-sepharose (GE Healthcare) polishing step to remove host cell protein contaminants ...
-
bioRxiv - Immunology 2024Quote: ... LP cells were further purified by gradient centrifugation at 2800 rpm for 20min with 44% and 67% Percoll (GE Healthcare/Cytiva) at room-temperature ...
-
bioRxiv - Immunology 2024Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy blood donors (provided by the Stockholm blood bank) through Ficoll-Hypaque (GE Healthcare) density-gradient centrifugation ...
-
bioRxiv - Biophysics 2022Quote: ... All BtuF constructs were purified directly from whole cell lysate (obtained by sonication) via Ni-NTA chromatography followed by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) in final buffers of 20-50 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2023Quote: ... was immobilised to approximately 2100 response units (RU) on a flow cell of a Streptavidin coated (SA) sensor chip (GE healthcare) according to manufacturer’s directions while another flow cell was left blank for reference subtraction and monitoring any non-specific bindings ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were centrifuged at 4°C to remove cell debris and the supernatant was filtered and loaded onto a Nickel Sepharose Fast Flow column (GE Healthcare) in the case of pSY10 constructs ...
-
bioRxiv - Microbiology 2023Quote: ... Mononuclear cells were collected and washed with saline to remove the platelets and then separated by centrifugation through a Percoll (GE Healthcare) gradient (40.48% and 47.70%) ...
-
bioRxiv - Microbiology 2023Quote: ... unpurified proteins contained in the supernatant of transfected HEK293F cells were immobilized on 2G12 or PGT145 functionalized CNBr-activated Sepharose 4B column (GE Healthcare) by flowing at 1 mL/min or overnight incubation at 4 °C on a roller bank ...
-
bioRxiv - Neuroscience 2023Quote: ... Erythroblasts were isolated when reaching 60-70% of the total cell population by gradient centrifugation at 1000 x g for 20 minutes at room temperature over Percoll (GE Healthcare). Isolated erythroblasts were frozen in FBS containing 10% DMSO at -80°C ...
-
bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
-
bioRxiv - Cell Biology 2023Quote: GST fusion protein was similarly purified using affinity chromatography by incubating bacterial cell lysate with glutathione-Sepharose 4B beads (#17075601; GE Healthcare) according to the manufacturer’s instructions and eluted from beads with elution buffer (50 mM Tris-HCl ...
-
bioRxiv - Biophysics 2022Quote: ... All BtuF constructs were purified directly from whole cell lysate (obtained by sonication) via Ni-NTA chromatography followed by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare) in final buffers of 20-50 mM Tris-HCl ...
-
bioRxiv - Cancer Biology 2022Quote: Primary human AML specimens and normal MPB samples were resuspended at about 100-200 e6 cells per ml in freezing media composed of 50% FBS (GE Healthcare), 10% DMSO (Sigma ...
-
bioRxiv - Bioengineering 2023Quote: ... Biomass concentrations were monitored using the cell growth quantifier (Scientific Bioprocessing) and determined manually by measuring the optical density at 600 nm with an Ultrospec 10 Cell Density Meter (GE Healthcare). The conversion factor of OD600 to cell dry weight (CDW ...