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Citations for GE Life Sciences :
3501 - 3550 of 4188 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... was used in combination with a secondary anti-mouse antibody conjugated to horseradish peroxidase (GE Healthcare NXA931, 1:5000). Primary rabbit anti-mCherry antibody (BioVision 5993 1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... a cell suspension containing 20,000 cells per 50uL was prepared in Biolog IF-M1 containing 0.3mM glutamine and 5% dialyzed FBS (dFBS) (Hyclone GE Life Sciences, SH30079.01) and plated into PM-M1 and PM-M2 96-well plates at 50 μL and 20,000 cells per well ...
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bioRxiv - Immunology 2021Quote: ... The lysate was centrifuged to remove cell debris and the supernatant was subjected to affinity chromatography using Ni-NTA beads (GE Life Sciences). The recombinant fusion proteins were eluted in 300 mM Imidazole in PBS pH 7.4while the protein was bound to the column by incubating at 4 °C for 12 to 16 h ...
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bioRxiv - Cell Biology 2020Quote: ... Imaging of MCF-10A cells was completed using the Applied Precision DeltaVision OMX fluorescent microscope (Applied Precision, GE Healthcare, Issaquah, WA, USA). Images shown are representative images at 60X magnification ...
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bioRxiv - Cell Biology 2020Quote: ... Imaging of MCF-10A cells was completed using the Applied Precision DeltaVision OMX fluorescent microscope (Applied Precision, GE Healthcare, Issaquah, WA, USA). Images shown are representative images at 60X magnification ...
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bioRxiv - Cell Biology 2020Quote: Total RNA from HCT116 cells were isolated using a lysis step based on guanidine thiocyanate (adapted from (53) and using magnetic beads (GE Healthcare, 65152105050450)) ...
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bioRxiv - Molecular Biology 2020Quote: ... Live cell imaging was performed using a DeltaVision microscope equipped with an incubation chamber and a CO2 supply (GE healthcare Life Sciences). Image analysis and quantification were performed using the Volocity software (PerkinElmer) ...
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bioRxiv - Neuroscience 2021Quote: ... Lipofectamine 2000 and plasmidic DNA were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Immunology 2022Quote: IgG was purified from cell culture media or plasma samples by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Genomics 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood by Ficoll density gradient centrifugation (p-05824, GE Healthcare, Illinois, US). The lymphocytes were simulated and transformed by treating cyclosporin A (No.12088 ...
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bioRxiv - Neuroscience 2020Quote: ... Lipofectamine 2000 and siRNAs were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Genetics 2019Quote: ... 293T cell line was cultured in DMEM high-glucose medium with 5% HyClone® fetal bovine serum (GE Healthcare, Piscataway, NJ, USA) and were maintained at 37°C with 5% CO2.
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bioRxiv - Cell Biology 2019Quote: ... hTert-RPE1 eGFP-HURP and hTert-RPE1 Centrin1-GFP/GFP-CENPA cells were recorded under same conditions using a Olympus DeltaVision wide-field microscope (GE Healthcare, Switzerland) equipped with a eGFP/RFP filter set (Chroma) ...
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bioRxiv - Cell Biology 2019Quote: Size exclusion chromatography experiments with HeLa cell extracts were performed with a pre-packed Superose 6 10/300 GL column (GE Healthcare ©). The column was equilibrated with buffer A (20 mM Hepes ...
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bioRxiv - Cancer Biology 2019Quote: ... we first isolated the peripheral blood mononuclear cells (PBMCs) from healthy donors by Ficoll-Paque PLUS density gradient centrifugation (GE Healthcare, USA), CD8+ T cells were sorted from PBMC by CD8 MicroBeads (human ...
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bioRxiv - Cell Biology 2020Quote: ... Live cell imaging was performed at 25°C (pre-calibrated chamber) on a Delta Vision Microscope (Applied Precision, GE Healthcare, Issaquah, WA) equipped with Olympus 60×/1.40 Plan-Apo objective lens ...
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bioRxiv - Cell Biology 2020Quote: ... Live cell imaging was performed at 25°C (pre-calibrated chamber) on a Delta Vision Microscope (Applied Precision, GE Healthcare, Issaquah, WA) equipped with Olympus 60×/1.40 Plan-Apo objective lens ...
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bioRxiv - Immunology 2021Quote: ... iPSC-moDC and MDM were washed with T cell expansion medium (RPMI supplemented with 10% HyClone FBS (GE Healthcare, Chicago, IL, USA), 1000 U/mL Penicillin/streptomycin ...
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bioRxiv - Microbiology 2021Quote: Whole cell protein lysate from 1 × 106 trypanosome cells was separated on 12.5% sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were blocked by incubation with 5% milk powder in PBS for 1 h at room temperature (RT ...
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bioRxiv - Biochemistry 2020Quote: ... The protein purification from precipitated cell-culture media was done on HisTrap HP 5 ml and HiLoad 16/600 Superdex 75 (GE Healthcare, USA) columns by the method described previously (Zahradník et al. ...
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bioRxiv - Biophysics 2021Quote: ... Bacterial cells were disrupted by sonication and the soluble fraction was loaded on a 5 mL HiTrap Chelating HP column (GE Healthcare - USA) previously equilibrated with same buffer ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from brain tissues or culture cells were extracted using the illustra RNAspin Mini RNA Isolation Kit (GE Healthcare Life Sciences) for reverse transcription with the High-Capacity cDNA Reverse Transcription Kits (ABI Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... and immediately processed for isolation of peripheral blood mononuclear cells (PBMCs) by density gradient centrifugation using Ficoll-Paque Premium 1.077 g/mL (GE Healthcare, IL; 17544202) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: For ratio imaging to assess differences in ROS content between esi-CTRL and esi-PDHB treatments in Hyper 7 expressing cells a Delta Vision Core wide-field deconvolution fluorescence microscope equipped with a CoolSNAP HQ2/HQ2-ICX285 camera (Applied Precision Inc.) and a UPlanSApo 40X/NA 0.95 lens (Olympus ...
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bioRxiv - Microbiology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were prepared from healthy individuals by centrifugation using Ficoll-Paque™ PLUS (GE Healthcare, Chicago, IL, USA). CD14+ cells were isolated by positive selection using anti-CD14 conjugated microbeads (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2021Quote: ... These cells were then visualised using FITC (495/519 nm) and DAPI (358/451 nm) channel using IN Cell Analyzer 6000 (GE Life Sciences) at 40x magnification.
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bioRxiv - Biochemistry 2020Quote: ... MALX-E-NirF was purified by loading the cleared cell lysate onto a 5ml MBPtrap HP column (GE Healthcare, Chicago, United States) which was eluted with a gradient to 20 mM maltose ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated either using RBC lysis or using density centrifugation with Ficoll-Paque PLUS (GE Healthcare, IL). RBC lysis was performed with a 45-minute incubation with Dextran T500 (Pharmacosmos ...
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bioRxiv - Cancer Biology 2021Quote: Multiplexed immunofluorescence iterative staining of the CRC TMAs was performed as previously described (Gerdes et al., 2013) using the Cell DIVE™ technology (Cytiva, Issaquah, WA; formerly GE Healthcare). This involves iterative staining and imaging of the same tissue section with 60+ antibodies and is achieved by mild dye oxidation between successive staining and imaging rounds ...
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bioRxiv - Microbiology 2020Quote: Cells were visualized on an Applied Precision DV Elite optical sectioning microscope with a Photometrics CoolSNAP-HQ2 camera (Applied Precision/GE Healthcare) was used to visualize the cells ...
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bioRxiv - Microbiology 2020Quote: Cells were visualized on an Applied Precision DV Elite optical sectioning microscope with a Photometrics CoolSNAP-HQ2 camera (Applied Precision/GE Healthcare) was used to visualize the cells ...
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bioRxiv - Immunology 2020Quote: ... and soluble HA trimers were purified from transfected cell supernatants by standard Ni-NTA chromatography using a prepacked HisTrap™ HP column (GE Healthcare) and SEC using a HiLoad® 16/600 Superdex® 200 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... Plates were then scanned (16 randomly selected fields/well at 20X objective) using the IN Cell Analyzer 2200 automated microscope (GE Healthcare, Singapore). T-cell migratory phenotypes and cell shapes were automatically quantified into calculated cell area ...
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bioRxiv - Immunology 2020Quote: ... and purified from clarified transfected cell supernatants four days post-transfection using HisTrap FF and HisTrap HP columns (GE Healthcare Life Sciences). After concentration ...
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bioRxiv - Immunology 2020Quote: Peripheral Blood Mononuclear Cells (PBMCs) were isolated from whole heparinised blood by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare, USA). An IFN-γ ELISpot assay was used to estimate the frequency and IFN-γ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human/simian IFN-γ kit (MabTech ...
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bioRxiv - Cancer Biology 2021Quote: ... His tagged bridging proteins were purified from the 293T transfected cell supernatant using a Ni Sepharose HisTrap excel column following the manufacturer’s protocol (GE Healthcare, Marlborough, MA). The relevant fractions were pooled and buffer exchanged to PBS.
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bioRxiv - Cell Biology 2022Quote: Super-resolution imaging of fixed cell samples with 3D-SIM was performed on a DeltaVision OMX V3 system (Applied Precision Imaging/GE Healthcare) equipped with a 100x/1.40 NA PlanApo oil immersion objective (Olympus) ...
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bioRxiv - Systems Biology 2022Quote: ... cells were fixed and immunostained for DENV E using the procedure as previously described.5 Images were acquired using the IN CELL Analyzer 3000 (INCA3000, GE Healthcare, USA) using a 40X magnifying objective allowing for nine images per well ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-231 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (cat# SH30243, Hyclone, GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated by density gradient centrifugation using the Ficoll® Paque Plus solution (17-1440-02, GE Healthcare).
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bioRxiv - Genetics 2023Quote: ... GST-Clr4 and MBP-Clr6 were expressed in Escherichia coli BL21 (DE3) cells and purified on Ni+ SepharoseTM 6 Fast Flow (for 6His fusions; GE Healthcare, 17531806), Glutathione SepharoseTM 4B (for GST fusions ...
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bioRxiv - Immunology 2023Quote: ... Vero cells for the neutralization activity test were maintained in the minimal essential medium (MEM) (GE Healthcare UK Ltd., Buckinghamshire, the UK) with 10% FBS ...
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bioRxiv - Genetics 2023Quote: ... The cultured cells were imaged live two days after transfection on an InCell Analyzer 6000 automated confocal fluorescence microscope (GE Healthcare Lifesciences).
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bioRxiv - Molecular Biology 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from the blood of healthy donors by gradient density centrifugation on Ficoll-Paque (GE Healthcare, USA) according to a standard protocol approved by the local ethics committee of the Dmitry Rogachev National Medical Research Center of Pediatric Hematology ...
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bioRxiv - Cell Biology 2024Quote: ... For western blots protein samples from 1 x 106 trypanosome cells were separated on 12.5 % sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). For protein dot blots ...
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bioRxiv - Plant Biology 2020Quote: ... 1 mM EDTA) affinity chromatography followed by size-exclusion chromatography on a HiLoad 16/600 Superdex 200pg column (GE Healthcare), equilibrated in 20 mM sodium citrate pH 5.0 ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Biophysics 2021Quote: ... Cell lysates were centrifuged at 75,000 x g for 25 min and supernatants were loaded on a GSTrap FF 1 mL column (GE Healthcare) using an AKTA Start purification system (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was then supplemented with 5 mM imidazole prior to loading onto a 1-ml HisTrap column (GE Healthcare) attached to an AKTA system at 1 ml/min ...